A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer
Prostate cancer is diagnosed in over 1 million men every year globally, yet current diagnostic modalities are inadequate for identification of significant cancer and more reliable early diagnostic biomarkers are necessary for improved clinical management of prostate cancer patients. MicroRNAs (miRNA...
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| Veröffentlicht in: | Scientific reports 2018-04, Vol.8 (1), p.6653-15, Article 6653 |
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| creator | Matin, Farhana Jeet, Varinder Moya, Leire Selth, Luke A. Chambers, Suzanne Clements, Judith A. Batra, Jyotsna |
| description | Prostate cancer is diagnosed in over 1 million men every year globally, yet current diagnostic modalities are inadequate for identification of significant cancer and more reliable early diagnostic biomarkers are necessary for improved clinical management of prostate cancer patients. MicroRNAs (miRNAs) modulate important cellular processes/pathways contributing to cancer and are stably present in body fluids. In this study we profiled 372 cancer-associated miRNAs in plasma collected before (~60% patients) and after/during commencement of treatment (~40% patients), from age-matched prostate cancer patients and healthy controls, and observed elevated levels of 4 miRNAs - miR-4289, miR-326, miR-152-3p and miR-98-5p, which were validated in an independent cohort. The miRNA panel was able to differentiate between prostate cancer patients and controls (AUC = 0.88). Analysis of published miRNA transcriptomic data from clinical samples demonstrated low expression of miR-152-3p in tumour compared to adjacent non-malignant tissues. Overexpression of miR-152-3p increased proliferation and migration of prostate cancer cells, suggesting a role for this miRNA in prostate cancer pathogenesis, a concept that was supported by pathway analysis of predicted miR-152-3p target genes. In summary, a four miRNA panel, including miR-152-3p which likely targets genes with key roles in prostate cancer pathogenesis, has the potential to improve early prostate cancer diagnosis. |
| doi_str_mv | 10.1038/s41598-018-24424-w |
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MicroRNAs (miRNAs) modulate important cellular processes/pathways contributing to cancer and are stably present in body fluids. In this study we profiled 372 cancer-associated miRNAs in plasma collected before (~60% patients) and after/during commencement of treatment (~40% patients), from age-matched prostate cancer patients and healthy controls, and observed elevated levels of 4 miRNAs - miR-4289, miR-326, miR-152-3p and miR-98-5p, which were validated in an independent cohort. The miRNA panel was able to differentiate between prostate cancer patients and controls (AUC = 0.88). Analysis of published miRNA transcriptomic data from clinical samples demonstrated low expression of miR-152-3p in tumour compared to adjacent non-malignant tissues. Overexpression of miR-152-3p increased proliferation and migration of prostate cancer cells, suggesting a role for this miRNA in prostate cancer pathogenesis, a concept that was supported by pathway analysis of predicted miR-152-3p target genes. In summary, a four miRNA panel, including miR-152-3p which likely targets genes with key roles in prostate cancer pathogenesis, has the potential to improve early prostate cancer diagnosis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-24424-w</identifier><identifier>PMID: 29703916</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/89 ; 38 ; 38/109 ; 38/39 ; 38/47 ; 38/77 ; 38/90 ; 45/41 ; 631/67/1059/602 ; 631/67/2322 ; 631/67/589/466 ; Biomarkers ; Body fluids ; Cell migration ; Cell proliferation ; Chromosome 3 ; Diagnosis ; Humanities and Social Sciences ; MicroRNAs ; miRNA ; multidisciplinary ; Pathogenesis ; Patients ; Prostate cancer ; Science ; Science (multidisciplinary) ; Tumors</subject><ispartof>Scientific reports, 2018-04, Vol.8 (1), p.6653-15, Article 6653</ispartof><rights>The Author(s) 2018</rights><rights>2018. 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MicroRNAs (miRNAs) modulate important cellular processes/pathways contributing to cancer and are stably present in body fluids. In this study we profiled 372 cancer-associated miRNAs in plasma collected before (~60% patients) and after/during commencement of treatment (~40% patients), from age-matched prostate cancer patients and healthy controls, and observed elevated levels of 4 miRNAs - miR-4289, miR-326, miR-152-3p and miR-98-5p, which were validated in an independent cohort. The miRNA panel was able to differentiate between prostate cancer patients and controls (AUC = 0.88). Analysis of published miRNA transcriptomic data from clinical samples demonstrated low expression of miR-152-3p in tumour compared to adjacent non-malignant tissues. Overexpression of miR-152-3p increased proliferation and migration of prostate cancer cells, suggesting a role for this miRNA in prostate cancer pathogenesis, a concept that was supported by pathway analysis of predicted miR-152-3p target genes. 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MicroRNAs (miRNAs) modulate important cellular processes/pathways contributing to cancer and are stably present in body fluids. In this study we profiled 372 cancer-associated miRNAs in plasma collected before (~60% patients) and after/during commencement of treatment (~40% patients), from age-matched prostate cancer patients and healthy controls, and observed elevated levels of 4 miRNAs - miR-4289, miR-326, miR-152-3p and miR-98-5p, which were validated in an independent cohort. The miRNA panel was able to differentiate between prostate cancer patients and controls (AUC = 0.88). Analysis of published miRNA transcriptomic data from clinical samples demonstrated low expression of miR-152-3p in tumour compared to adjacent non-malignant tissues. Overexpression of miR-152-3p increased proliferation and migration of prostate cancer cells, suggesting a role for this miRNA in prostate cancer pathogenesis, a concept that was supported by pathway analysis of predicted miR-152-3p target genes. In summary, a four miRNA panel, including miR-152-3p which likely targets genes with key roles in prostate cancer pathogenesis, has the potential to improve early prostate cancer diagnosis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29703916</pmid><doi>10.1038/s41598-018-24424-w</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-4646-6247</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/89 38 38/109 38/39 38/47 38/77 38/90 45/41 631/67/1059/602 631/67/2322 631/67/589/466 Biomarkers Body fluids Cell migration Cell proliferation Chromosome 3 Diagnosis Humanities and Social Sciences MicroRNAs miRNA multidisciplinary Pathogenesis Patients Prostate cancer Science Science (multidisciplinary) Tumors |
| title | A Plasma Biomarker Panel of Four MicroRNAs for the Diagnosis of Prostate Cancer |
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