In Planta Processing of the SpCas9-gRNA Complex

In CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9)-mediated genome editing in plants, Streptococcus pyogenes Cas9 (SpCas9) protein and the required guide RNA (gRNA) are, in most cases, expressed from a stably integrated transgene. Generally, SpCas9...

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Veröffentlicht in:	Plant and cell physiology 2017-11, Vol.58 (11), p.1857-1867
Hauptverfasser:	Mikami, Masafumi, Toki, Seiichi, Endo, Masaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis - genetics Bacterial Proteins - genetics Bacterial Proteins - metabolism CRISPR-Associated Protein 9 CRISPR-Cas Systems DNA Polymerase II - genetics Endonucleases - genetics Endonucleases - metabolism Mutagenesis, Site-Directed Mutation Oryza - genetics Plants, Genetically Modified Promoter Regions, Genetic Rapid Papers RNA, Catalytic - genetics RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism
Online-Zugang:	Volltext
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