Oncogene Amplification in Squamous Cell Carcinoma of the Oral Cavity
We have determined the prevalence of amplification of c‐myc, N‐myc, L‐myc, H‐ras, Ki‐ras, and N‐ras oncogenes in 23 cases of squamous cell carcinoma of the oral cavity, using Southern hybridization analysis of DNA extracted from the primary tumor tissues. Nick‐translated oncogene probes and oncogene...
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| Veröffentlicht in: | Cancer science 1989-05, Vol.80 (5), p.430-437 |
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| creator | Saranath, Dhananjaya Panchal, Rekha G. Nair, Rama Mehta, Ashok R. Sanghavi, Vikram Sumegi, Janos Klein, George Deo, Madhav G. |
| description | We have determined the prevalence of amplification of c‐myc, N‐myc, L‐myc, H‐ras, Ki‐ras, and N‐ras oncogenes in 23 cases of squamous cell carcinoma of the oral cavity, using Southern hybridization analysis of DNA extracted from the primary tumor tissues. Nick‐translated oncogene probes and oncogene inserts labeled to high specific activities were used. We observed a 5‐ to 10‐fold amplification of one or more of c‐myc, N‐myc, Ki‐ras and N‐ras oncogenes in 56% of the tumor tissue samples, with these oncogenes not being amplified in the peripheral blood cells of the same patients, L‐myc and H‐ras were not amplified in any of our samples. The oncogene amplifications seemed to be associated with advanced stages of squamous cell carcinomas, with the ras and myc family oncogenes being amplified in stages 3 and 4. Hybridization with N‐myc detected an additional 2.3 kb EcoRI fragment, along with the normal 2.1 kb fragment. Our data also demonstrated amplification of multiple oncogenes in the same tumor tissue sample. About 60% of the samples with amplified oncogenes showed simultaneous amplification of 2 or more oncogenes. The results showing different oncogene amplifications in similar tumors, as well as multiple oncogene amplifications in the same tumor, suggest that these oncogenes may be alternatively or simultaneously activated in oral carcinogenesis. |
| doi_str_mv | 10.1111/j.1349-7006.1989.tb02332.x |
| format | Article |
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Nick‐translated oncogene probes and oncogene inserts labeled to high specific activities were used. We observed a 5‐ to 10‐fold amplification of one or more of c‐myc, N‐myc, Ki‐ras and N‐ras oncogenes in 56% of the tumor tissue samples, with these oncogenes not being amplified in the peripheral blood cells of the same patients, L‐myc and H‐ras were not amplified in any of our samples. The oncogene amplifications seemed to be associated with advanced stages of squamous cell carcinomas, with the ras and myc family oncogenes being amplified in stages 3 and 4. Hybridization with N‐myc detected an additional 2.3 kb EcoRI fragment, along with the normal 2.1 kb fragment. Our data also demonstrated amplification of multiple oncogenes in the same tumor tissue sample. About 60% of the samples with amplified oncogenes showed simultaneous amplification of 2 or more oncogenes. The results showing different oncogene amplifications in similar tumors, as well as multiple oncogene amplifications in the same tumor, suggest that these oncogenes may be alternatively or simultaneously activated in oral carcinogenesis.</description><identifier>ISSN: 0910-5050</identifier><identifier>ISSN: 1347-9032</identifier><identifier>EISSN: 1349-7006</identifier><identifier>EISSN: 1876-4673</identifier><identifier>DOI: 10.1111/j.1349-7006.1989.tb02332.x</identifier><identifier>PMID: 2502519</identifier><identifier>CODEN: GANNA2</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Amplification ; Biological and medical sciences ; Blood cells ; Carcinogenesis ; Carcinoma, Squamous Cell - genetics ; DNA probes ; Female ; Gene Amplification ; Humans ; Hybridization analysis ; Male ; Medical sciences ; Middle Aged ; Mouth Neoplasms - genetics ; Myc protein ; Oncogene ; Oncogenes ; Oral cancer ; Oral cavity ; Otorhinolaryngology. Stomatology ; Peripheral blood ; Proto-Oncogenes ; Squamous cell carcinoma ; Tumors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>Cancer science, 1989-05, Vol.80 (5), p.430-437</ispartof><rights>1989 INIST-CNRS</rights><rights>Copyright John Wiley & Sons, Inc. May 1989</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6300-c25eb6d570a284d917a2106bcd9a2dbffaa3ca1250317e982968b85bec4a07b3</citedby><cites>FETCH-LOGICAL-c6300-c25eb6d570a284d917a2106bcd9a2dbffaa3ca1250317e982968b85bec4a07b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917753/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5917753/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,27901,27902,45550,45551,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7284949$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2502519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saranath, Dhananjaya</creatorcontrib><creatorcontrib>Panchal, Rekha G.</creatorcontrib><creatorcontrib>Nair, Rama</creatorcontrib><creatorcontrib>Mehta, Ashok R.</creatorcontrib><creatorcontrib>Sanghavi, Vikram</creatorcontrib><creatorcontrib>Sumegi, Janos</creatorcontrib><creatorcontrib>Klein, George</creatorcontrib><creatorcontrib>Deo, Madhav G.</creatorcontrib><title>Oncogene Amplification in Squamous Cell Carcinoma of the Oral Cavity</title><title>Cancer science</title><addtitle>Jpn J Cancer Res</addtitle><description>We have determined the prevalence of amplification of c‐myc, N‐myc, L‐myc, H‐ras, Ki‐ras, and N‐ras oncogenes in 23 cases of squamous cell carcinoma of the oral cavity, using Southern hybridization analysis of DNA extracted from the primary tumor tissues. Nick‐translated oncogene probes and oncogene inserts labeled to high specific activities were used. We observed a 5‐ to 10‐fold amplification of one or more of c‐myc, N‐myc, Ki‐ras and N‐ras oncogenes in 56% of the tumor tissue samples, with these oncogenes not being amplified in the peripheral blood cells of the same patients, L‐myc and H‐ras were not amplified in any of our samples. The oncogene amplifications seemed to be associated with advanced stages of squamous cell carcinomas, with the ras and myc family oncogenes being amplified in stages 3 and 4. Hybridization with N‐myc detected an additional 2.3 kb EcoRI fragment, along with the normal 2.1 kb fragment. Our data also demonstrated amplification of multiple oncogenes in the same tumor tissue sample. About 60% of the samples with amplified oncogenes showed simultaneous amplification of 2 or more oncogenes. The results showing different oncogene amplifications in similar tumors, as well as multiple oncogene amplifications in the same tumor, suggest that these oncogenes may be alternatively or simultaneously activated in oral carcinogenesis.</description><subject>Adult</subject><subject>Amplification</subject><subject>Biological and medical sciences</subject><subject>Blood cells</subject><subject>Carcinogenesis</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>DNA probes</subject><subject>Female</subject><subject>Gene Amplification</subject><subject>Humans</subject><subject>Hybridization analysis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mouth Neoplasms - genetics</subject><subject>Myc protein</subject><subject>Oncogene</subject><subject>Oncogenes</subject><subject>Oral cancer</subject><subject>Oral cavity</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Peripheral blood</subject><subject>Proto-Oncogenes</subject><subject>Squamous cell carcinoma</subject><subject>Tumors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>0910-5050</issn><issn>1347-9032</issn><issn>1349-7006</issn><issn>1876-4673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVUU1v1DAUtBCoLIWfgBQB6i3h2Y6TmAPSKi0fUqU90Lv14jitV4m9jZPS_fc4arR83PDFkmfeeOYNIe8oZDSej_uM8lymJUCRUVnJbGqAcc6yx2dkc4Kekw1ICqkAAS_JqxD2ALSEgp2RMyaACSo35HLntL81ziTb4dDbzmqcrHeJdcmP-xkHP4ekNn2f1Dhq6_yAie-S6c4kuxGX1wc7HV-TFx32wbxZ73Ny8-Xqpv6WXu--fq-316kuOECqmTBN0YoSkFV5K2mJjELR6FYia5uuQ-QaafTGaWlkxWRRNZVojM4Ryoafk89Psoe5GUyrjZuiB3UY7YDjUXm06m_E2Tt16x-UiF-VgkeBi1Vg9PezCZMabNAxHToTgyoqcso4LMT3_xD3fh5dzKYYl7LgkkEVWZ-eWHr0IYymO1mhoJai1F4tbailDbUUpdai1GMcfvtnmNPo2kzEP6w4Bo19N6LTNpxoZdygzOXvnfy0vTn-hwFVb6_ymPUXlHSvhw</recordid><startdate>198905</startdate><enddate>198905</enddate><creator>Saranath, Dhananjaya</creator><creator>Panchal, Rekha G.</creator><creator>Nair, Rama</creator><creator>Mehta, Ashok R.</creator><creator>Sanghavi, Vikram</creator><creator>Sumegi, Janos</creator><creator>Klein, George</creator><creator>Deo, Madhav G.</creator><general>Blackwell Publishing Ltd</general><general>Japanese Cancer Association</general><general>John Wiley & Sons, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TO</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>198905</creationdate><title>Oncogene Amplification in Squamous Cell Carcinoma of the Oral Cavity</title><author>Saranath, Dhananjaya ; Panchal, Rekha G. ; Nair, Rama ; Mehta, Ashok R. ; Sanghavi, Vikram ; Sumegi, Janos ; Klein, George ; Deo, Madhav G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6300-c25eb6d570a284d917a2106bcd9a2dbffaa3ca1250317e982968b85bec4a07b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adult</topic><topic>Amplification</topic><topic>Biological and medical sciences</topic><topic>Blood cells</topic><topic>Carcinogenesis</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>DNA probes</topic><topic>Female</topic><topic>Gene Amplification</topic><topic>Humans</topic><topic>Hybridization analysis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mouth Neoplasms - genetics</topic><topic>Myc protein</topic><topic>Oncogene</topic><topic>Oncogenes</topic><topic>Oral cancer</topic><topic>Oral cavity</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Peripheral blood</topic><topic>Proto-Oncogenes</topic><topic>Squamous cell carcinoma</topic><topic>Tumors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saranath, Dhananjaya</creatorcontrib><creatorcontrib>Panchal, Rekha G.</creatorcontrib><creatorcontrib>Nair, Rama</creatorcontrib><creatorcontrib>Mehta, Ashok R.</creatorcontrib><creatorcontrib>Sanghavi, Vikram</creatorcontrib><creatorcontrib>Sumegi, Janos</creatorcontrib><creatorcontrib>Klein, George</creatorcontrib><creatorcontrib>Deo, Madhav G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saranath, Dhananjaya</au><au>Panchal, Rekha G.</au><au>Nair, Rama</au><au>Mehta, Ashok R.</au><au>Sanghavi, Vikram</au><au>Sumegi, Janos</au><au>Klein, George</au><au>Deo, Madhav G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oncogene Amplification in Squamous Cell Carcinoma of the Oral Cavity</atitle><jtitle>Cancer science</jtitle><addtitle>Jpn J Cancer Res</addtitle><date>1989-05</date><risdate>1989</risdate><volume>80</volume><issue>5</issue><spage>430</spage><epage>437</epage><pages>430-437</pages><issn>0910-5050</issn><issn>1347-9032</issn><eissn>1349-7006</eissn><eissn>1876-4673</eissn><coden>GANNA2</coden><abstract>We have determined the prevalence of amplification of c‐myc, N‐myc, L‐myc, H‐ras, Ki‐ras, and N‐ras oncogenes in 23 cases of squamous cell carcinoma of the oral cavity, using Southern hybridization analysis of DNA extracted from the primary tumor tissues. Nick‐translated oncogene probes and oncogene inserts labeled to high specific activities were used. We observed a 5‐ to 10‐fold amplification of one or more of c‐myc, N‐myc, Ki‐ras and N‐ras oncogenes in 56% of the tumor tissue samples, with these oncogenes not being amplified in the peripheral blood cells of the same patients, L‐myc and H‐ras were not amplified in any of our samples. The oncogene amplifications seemed to be associated with advanced stages of squamous cell carcinomas, with the ras and myc family oncogenes being amplified in stages 3 and 4. Hybridization with N‐myc detected an additional 2.3 kb EcoRI fragment, along with the normal 2.1 kb fragment. Our data also demonstrated amplification of multiple oncogenes in the same tumor tissue sample. About 60% of the samples with amplified oncogenes showed simultaneous amplification of 2 or more oncogenes. The results showing different oncogene amplifications in similar tumors, as well as multiple oncogene amplifications in the same tumor, suggest that these oncogenes may be alternatively or simultaneously activated in oral carcinogenesis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2502519</pmid><doi>10.1111/j.1349-7006.1989.tb02332.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Amplification Biological and medical sciences Blood cells Carcinogenesis Carcinoma, Squamous Cell - genetics DNA probes Female Gene Amplification Humans Hybridization analysis Male Medical sciences Middle Aged Mouth Neoplasms - genetics Myc protein Oncogene Oncogenes Oral cancer Oral cavity Otorhinolaryngology. Stomatology Peripheral blood Proto-Oncogenes Squamous cell carcinoma Tumors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Oncogene Amplification in Squamous Cell Carcinoma of the Oral Cavity |
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