Counter-ion effect on antistaphylococcal activity and cytotoxicity of selected antimicrobial peptides
In view of an appreciable increase in resistance of Staphylococcus aureus to the conventional antibiotics, it is desired to develop new effective drugs. Antimicrobial peptides (AMPs) seem to be attractive candidates. In general, AMPs samples used for in vitro studies consist of a peptide, counter-io...
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| Veröffentlicht in: | Amino acids 2018-05, Vol.50 (5), p.609-619 |
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| description | In view of an appreciable increase in resistance of
Staphylococcus aureus
to the conventional antibiotics, it is desired to develop new effective drugs. Antimicrobial peptides (AMPs) seem to be attractive candidates. In general, AMPs samples used for in vitro studies consist of a peptide, counter-ion, and water. The presence of the counter-ion could be significant as it affects peptide secondary structure and biological activity. The purpose of this study was to estimate the impact of counter-ion on antistaphylococcal activity of selected AMPs (CAMEL, citropin 1.1, LL-37, pexiganan, temporin A). To do this, three kinds of salts were prepared, namely, acetates, hydrochlorides, and trifluoroacetates. In addition, the hemolytic activity against human red blood cells (hRBCs) and cytotoxicity (HaCaT) were determined. The results indicate that there is a substantial difference between different salts, but the pattern is not consistent for the peptides. In general, the antistaphylococcal activity decreased in the order: CAMEL > temporin A > pexiganan > citropin 1.1 ≫ LL-37. The highest selectivity indexes were determined for CAMEL hydrochloride, pexiganan acetate, and temporin A trifluoroacetate. This study shows how important is to take into account the kind of counter-ions when designing novel peptide-based antimicrobials. |
| doi_str_mv | 10.1007/s00726-017-2536-9 |
| format | Article |
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Staphylococcus aureus
to the conventional antibiotics, it is desired to develop new effective drugs. Antimicrobial peptides (AMPs) seem to be attractive candidates. In general, AMPs samples used for in vitro studies consist of a peptide, counter-ion, and water. The presence of the counter-ion could be significant as it affects peptide secondary structure and biological activity. The purpose of this study was to estimate the impact of counter-ion on antistaphylococcal activity of selected AMPs (CAMEL, citropin 1.1, LL-37, pexiganan, temporin A). To do this, three kinds of salts were prepared, namely, acetates, hydrochlorides, and trifluoroacetates. In addition, the hemolytic activity against human red blood cells (hRBCs) and cytotoxicity (HaCaT) were determined. The results indicate that there is a substantial difference between different salts, but the pattern is not consistent for the peptides. In general, the antistaphylococcal activity decreased in the order: CAMEL > temporin A > pexiganan > citropin 1.1 ≫ LL-37. The highest selectivity indexes were determined for CAMEL hydrochloride, pexiganan acetate, and temporin A trifluoroacetate. This study shows how important is to take into account the kind of counter-ions when designing novel peptide-based antimicrobials.</description><identifier>ISSN: 0939-4451</identifier><identifier>EISSN: 1438-2199</identifier><identifier>DOI: 10.1007/s00726-017-2536-9</identifier><identifier>PMID: 29307075</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Acetates ; Acetic acid ; Analytical Chemistry ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Antiinfectives and antibacterials ; Antimicrobial agents ; Antimicrobial Cationic Peptides - chemistry ; Antimicrobial Cationic Peptides - pharmacology ; Antimicrobial peptides ; Biochemical Engineering ; Biochemistry ; Biological activity ; Biomedical and Life Sciences ; Cations - chemistry ; Cations - pharmacology ; Cytotoxicity ; Drug development ; Erythrocytes ; Hydrochlorides ; Life Sciences ; Neurobiology ; Original ; Original Article ; Pathogens ; Peptides ; Protein structure ; Proteomics ; Salts ; Secondary structure ; Staphylococcus aureus - growth & development ; Toxicity ; Trifluoroacetates</subject><ispartof>Amino acids, 2018-05, Vol.50 (5), p.609-619</ispartof><rights>The Author(s) 2018</rights><rights>Amino Acids is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-716a859f9e31990c83460023248aed77a9f0d8ac106e87797ee8563174311d693</citedby><cites>FETCH-LOGICAL-c470t-716a859f9e31990c83460023248aed77a9f0d8ac106e87797ee8563174311d693</cites><orcidid>0000-0003-0275-3017</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00726-017-2536-9$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00726-017-2536-9$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29307075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sikora, Karol</creatorcontrib><creatorcontrib>Jaśkiewicz, Maciej</creatorcontrib><creatorcontrib>Neubauer, Damian</creatorcontrib><creatorcontrib>Bauer, Marta</creatorcontrib><creatorcontrib>Bartoszewska, Sylwia</creatorcontrib><creatorcontrib>Barańska-Rybak, Wioletta</creatorcontrib><creatorcontrib>Kamysz, Wojciech</creatorcontrib><title>Counter-ion effect on antistaphylococcal activity and cytotoxicity of selected antimicrobial peptides</title><title>Amino acids</title><addtitle>Amino Acids</addtitle><addtitle>Amino Acids</addtitle><description>In view of an appreciable increase in resistance of
Staphylococcus aureus
to the conventional antibiotics, it is desired to develop new effective drugs. Antimicrobial peptides (AMPs) seem to be attractive candidates. In general, AMPs samples used for in vitro studies consist of a peptide, counter-ion, and water. The presence of the counter-ion could be significant as it affects peptide secondary structure and biological activity. The purpose of this study was to estimate the impact of counter-ion on antistaphylococcal activity of selected AMPs (CAMEL, citropin 1.1, LL-37, pexiganan, temporin A). To do this, three kinds of salts were prepared, namely, acetates, hydrochlorides, and trifluoroacetates. In addition, the hemolytic activity against human red blood cells (hRBCs) and cytotoxicity (HaCaT) were determined. The results indicate that there is a substantial difference between different salts, but the pattern is not consistent for the peptides. In general, the antistaphylococcal activity decreased in the order: CAMEL > temporin A > pexiganan > citropin 1.1 ≫ LL-37. The highest selectivity indexes were determined for CAMEL hydrochloride, pexiganan acetate, and temporin A trifluoroacetate. This study shows how important is to take into account the kind of counter-ions when designing novel peptide-based antimicrobials.</description><subject>Acetates</subject><subject>Acetic acid</subject><subject>Analytical Chemistry</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial Cationic Peptides - chemistry</subject><subject>Antimicrobial Cationic Peptides - pharmacology</subject><subject>Antimicrobial peptides</subject><subject>Biochemical Engineering</subject><subject>Biochemistry</subject><subject>Biological activity</subject><subject>Biomedical and Life Sciences</subject><subject>Cations - chemistry</subject><subject>Cations - pharmacology</subject><subject>Cytotoxicity</subject><subject>Drug development</subject><subject>Erythrocytes</subject><subject>Hydrochlorides</subject><subject>Life Sciences</subject><subject>Neurobiology</subject><subject>Original</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Peptides</subject><subject>Protein structure</subject><subject>Proteomics</subject><subject>Salts</subject><subject>Secondary structure</subject><subject>Staphylococcus aureus - 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chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial Cationic Peptides - chemistry</topic><topic>Antimicrobial Cationic Peptides - pharmacology</topic><topic>Antimicrobial peptides</topic><topic>Biochemical Engineering</topic><topic>Biochemistry</topic><topic>Biological activity</topic><topic>Biomedical and Life Sciences</topic><topic>Cations - chemistry</topic><topic>Cations - pharmacology</topic><topic>Cytotoxicity</topic><topic>Drug development</topic><topic>Erythrocytes</topic><topic>Hydrochlorides</topic><topic>Life Sciences</topic><topic>Neurobiology</topic><topic>Original</topic><topic>Original Article</topic><topic>Pathogens</topic><topic>Peptides</topic><topic>Protein structure</topic><topic>Proteomics</topic><topic>Salts</topic><topic>Secondary structure</topic><topic>Staphylococcus aureus - growth & development</topic><topic>Toxicity</topic><topic>Trifluoroacetates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sikora, Karol</creatorcontrib><creatorcontrib>Jaśkiewicz, Maciej</creatorcontrib><creatorcontrib>Neubauer, Damian</creatorcontrib><creatorcontrib>Bauer, Marta</creatorcontrib><creatorcontrib>Bartoszewska, Sylwia</creatorcontrib><creatorcontrib>Barańska-Rybak, Wioletta</creatorcontrib><creatorcontrib>Kamysz, Wojciech</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Amino acids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sikora, Karol</au><au>Jaśkiewicz, Maciej</au><au>Neubauer, Damian</au><au>Bauer, Marta</au><au>Bartoszewska, Sylwia</au><au>Barańska-Rybak, Wioletta</au><au>Kamysz, Wojciech</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Counter-ion effect on antistaphylococcal activity and cytotoxicity of selected antimicrobial peptides</atitle><jtitle>Amino acids</jtitle><stitle>Amino Acids</stitle><addtitle>Amino Acids</addtitle><date>2018-05-01</date><risdate>2018</risdate><volume>50</volume><issue>5</issue><spage>609</spage><epage>619</epage><pages>609-619</pages><issn>0939-4451</issn><eissn>1438-2199</eissn><abstract>In view of an appreciable increase in resistance of
Staphylococcus aureus
to the conventional antibiotics, it is desired to develop new effective drugs. Antimicrobial peptides (AMPs) seem to be attractive candidates. In general, AMPs samples used for in vitro studies consist of a peptide, counter-ion, and water. The presence of the counter-ion could be significant as it affects peptide secondary structure and biological activity. The purpose of this study was to estimate the impact of counter-ion on antistaphylococcal activity of selected AMPs (CAMEL, citropin 1.1, LL-37, pexiganan, temporin A). To do this, three kinds of salts were prepared, namely, acetates, hydrochlorides, and trifluoroacetates. In addition, the hemolytic activity against human red blood cells (hRBCs) and cytotoxicity (HaCaT) were determined. The results indicate that there is a substantial difference between different salts, but the pattern is not consistent for the peptides. In general, the antistaphylococcal activity decreased in the order: CAMEL > temporin A > pexiganan > citropin 1.1 ≫ LL-37. The highest selectivity indexes were determined for CAMEL hydrochloride, pexiganan acetate, and temporin A trifluoroacetate. This study shows how important is to take into account the kind of counter-ions when designing novel peptide-based antimicrobials.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>29307075</pmid><doi>10.1007/s00726-017-2536-9</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-0275-3017</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Amino acids, 2018-05, Vol.50 (5), p.609-619 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Acetates Acetic acid Analytical Chemistry Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibiotics Antiinfectives and antibacterials Antimicrobial agents Antimicrobial Cationic Peptides - chemistry Antimicrobial Cationic Peptides - pharmacology Antimicrobial peptides Biochemical Engineering Biochemistry Biological activity Biomedical and Life Sciences Cations - chemistry Cations - pharmacology Cytotoxicity Drug development Erythrocytes Hydrochlorides Life Sciences Neurobiology Original Original Article Pathogens Peptides Protein structure Proteomics Salts Secondary structure Staphylococcus aureus - growth & development Toxicity Trifluoroacetates |
| title | Counter-ion effect on antistaphylococcal activity and cytotoxicity of selected antimicrobial peptides |
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