Identification of a New Glucosinolate-Rich Cell Type in Arabidopsis Flower Stalk
Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differentia...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2000-10, Vol.124 (2), p.599-608 |
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| creator | Koroleva, Olga A. Davies, Andrew Deeken, Rosalia Thorpe, Michael R. Tomos, A. Deri Hedrich, Rainer |
| description | Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differential pattern of distribution for all five elements. K concentration was found to be highest in the parenchymatous tissue around vascular bundles. Ca and Cl were present mainly in the central part of the flower stalk. P was largely located in the bundles and in the parenchyma surrounding them. S signal was extraordinary high in groups of cells (S-cells) situated between the phloem of every vascular bundle and the endodermis. Enzymatic hydrolysis by thioglucosidase of cell sap collected from S-cells using a glass microcapillary resulted in the release of glucose, indicating that these cells contain glucosinolates at high (> 100 mM) concentration, which is consistent with the concentration of S (> 200 mM) estimated by x-ray analysis of cell sap samples. Since their position outside of the phloem is ideally suited for protecting the long-distance transport system from feeding insects, the possible roles of these cells as components of a plant defense system are discussed. |
| doi_str_mv | 10.1104/pp.124.2.599 |
| format | Article |
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Enzymatic hydrolysis by thioglucosidase of cell sap collected from S-cells using a glass microcapillary resulted in the release of glucose, indicating that these cells contain glucosinolates at high (> 100 mM) concentration, which is consistent with the concentration of S (> 200 mM) estimated by x-ray analysis of cell sap samples. Since their position outside of the phloem is ideally suited for protecting the long-distance transport system from feeding insects, the possible roles of these cells as components of a plant defense system are discussed.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.124.2.599</identifier><identifier>PMID: 11027710</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Agronomy. Soil science and plant productions ; Arabidopsis - anatomy & histology ; Arabidopsis - cytology ; Arabidopsis - metabolism ; Biological and medical sciences ; Cell Biology and Signal Transduction ; Cells ; Economic plant physiology ; Electron Probe Microanalysis ; Epidermal cells ; Flowers ; Fundamental and applied biological sciences. Psychology ; Glucosinolates ; Glucosinolates - metabolism ; Metabolism ; Metabolism. Physicochemical requirements ; Nitrogen metabolism and other ones (excepting carbon metabolism) ; Nutrition. Photosynthesis. Respiration. 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Deri</creatorcontrib><creatorcontrib>Hedrich, Rainer</creatorcontrib><title>Identification of a New Glucosinolate-Rich Cell Type in Arabidopsis Flower Stalk</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differential pattern of distribution for all five elements. K concentration was found to be highest in the parenchymatous tissue around vascular bundles. Ca and Cl were present mainly in the central part of the flower stalk. P was largely located in the bundles and in the parenchyma surrounding them. S signal was extraordinary high in groups of cells (S-cells) situated between the phloem of every vascular bundle and the endodermis. Enzymatic hydrolysis by thioglucosidase of cell sap collected from S-cells using a glass microcapillary resulted in the release of glucose, indicating that these cells contain glucosinolates at high (> 100 mM) concentration, which is consistent with the concentration of S (> 200 mM) estimated by x-ray analysis of cell sap samples. Since their position outside of the phloem is ideally suited for protecting the long-distance transport system from feeding insects, the possible roles of these cells as components of a plant defense system are discussed.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Arabidopsis - anatomy & histology</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Biology and Signal Transduction</subject><subject>Cells</subject><subject>Economic plant physiology</subject><subject>Electron Probe Microanalysis</subject><subject>Epidermal cells</subject><subject>Flowers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucosinolates</subject><subject>Glucosinolates - metabolism</subject><subject>Metabolism</subject><subject>Metabolism. Physicochemical requirements</subject><subject>Nitrogen metabolism and other ones (excepting carbon metabolism)</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>Parenchyma</subject><subject>Phloem</subject><subject>Pith</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Tissue Distribution</subject><subject>Vascular bundles</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpVkFFLHDEUhYNY6tb2zcciofSxs-ZmkkkCfZGlWkFaae1zSDKZmjVOxmS24r9vyi5qn-6F-53DuQehIyBLAMJOpmkJlC3pkiu1hxbAW9pQzuQ-WhBSdyKlOkBvSlkTQqAF9hodVCEVAsgCXV30fpzDEJyZQxpxGrDB3_wDPo8bl0oYUzSzb34Ed4NXPkZ8_Th5HEZ8mo0NfZpKKPgspgef8c_ZxNu36NVgYvHvdvMQ_Tr7cr362lx-P79YnV42jnMxN2CZZa0UiijWU-UFVc5J4YSxrifeDcaD7QfZeUJUbzkB0TLJuSVqcN727SH6vPWdNvbO965-kU3UUw53Jj_qZIL-_zKGG_07_dFcQddV-YedPKf7jS-zXqdNHmtiTUF2AAxkhT5tIZdTKdkPT_ZA9L_y9TTpWr6m1VVV_PhlpGd413YFPu4AU5yJQzajC-WZ4yAAaMXeb7F1mVN-OjMqFOto-xfCuZbw</recordid><startdate>20001001</startdate><enddate>20001001</enddate><creator>Koroleva, Olga A.</creator><creator>Davies, Andrew</creator><creator>Deeken, Rosalia</creator><creator>Thorpe, Michael R.</creator><creator>Tomos, A. 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Physicochemical requirements</topic><topic>Nitrogen metabolism and other ones (excepting carbon metabolism)</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>Parenchyma</topic><topic>Phloem</topic><topic>Pith</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Tissue Distribution</topic><topic>Vascular bundles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koroleva, Olga A.</creatorcontrib><creatorcontrib>Davies, Andrew</creatorcontrib><creatorcontrib>Deeken, Rosalia</creatorcontrib><creatorcontrib>Thorpe, Michael R.</creatorcontrib><creatorcontrib>Tomos, A. 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Deri</au><au>Hedrich, Rainer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a New Glucosinolate-Rich Cell Type in Arabidopsis Flower Stalk</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2000-10-01</date><risdate>2000</risdate><volume>124</volume><issue>2</issue><spage>599</spage><epage>608</epage><pages>599-608</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differential pattern of distribution for all five elements. K concentration was found to be highest in the parenchymatous tissue around vascular bundles. Ca and Cl were present mainly in the central part of the flower stalk. P was largely located in the bundles and in the parenchyma surrounding them. S signal was extraordinary high in groups of cells (S-cells) situated between the phloem of every vascular bundle and the endodermis. Enzymatic hydrolysis by thioglucosidase of cell sap collected from S-cells using a glass microcapillary resulted in the release of glucose, indicating that these cells contain glucosinolates at high (> 100 mM) concentration, which is consistent with the concentration of S (> 200 mM) estimated by x-ray analysis of cell sap samples. Since their position outside of the phloem is ideally suited for protecting the long-distance transport system from feeding insects, the possible roles of these cells as components of a plant defense system are discussed.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>11027710</pmid><doi>10.1104/pp.124.2.599</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Agronomy. Soil science and plant productions Arabidopsis - anatomy & histology Arabidopsis - cytology Arabidopsis - metabolism Biological and medical sciences Cell Biology and Signal Transduction Cells Economic plant physiology Electron Probe Microanalysis Epidermal cells Flowers Fundamental and applied biological sciences. Psychology Glucosinolates Glucosinolates - metabolism Metabolism Metabolism. Physicochemical requirements Nitrogen metabolism and other ones (excepting carbon metabolism) Nutrition. Photosynthesis. Respiration. Metabolism Parenchyma Phloem Pith Plant cells Plant physiology and development Plants Tissue Distribution Vascular bundles |
| title | Identification of a New Glucosinolate-Rich Cell Type in Arabidopsis Flower Stalk |
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