Identification and Optimization of 4‐Anilinoquinolines as Inhibitors of Cyclin G Associated Kinase
4‐Anilinoquinolines were identified as potent and narrow‐spectrum inhibitors of the cyclin G associated kinase (GAK), an important regulator of viral and bacterial entry into host cells. Optimization of the 4‐anilino group and the 6,7‐quinoline substituents produced GAK inhibitors with nanomolar act...
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| Veröffentlicht in: | ChemMedChem 2018-01, Vol.13 (1), p.48-66 |
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| creator | Asquith, Christopher R. M. Laitinen, Tuomo Bennett, James M. Godoi, Paulo H. East, Michael P. Tizzard, Graham J. Graves, Lee M. Johnson, Gary L. Dornsife, Ronna E. Wells, Carrow I. Elkins, Jonathan M. Willson, Timothy M. Zuercher, William J. |
| description | 4‐Anilinoquinolines were identified as potent and narrow‐spectrum inhibitors of the cyclin G associated kinase (GAK), an important regulator of viral and bacterial entry into host cells. Optimization of the 4‐anilino group and the 6,7‐quinoline substituents produced GAK inhibitors with nanomolar activity, over 50 000‐fold selectivity relative to other members of the numb‐associated kinase (NAK) subfamily, and a compound (6,7‐dimethoxy‐N‐(3,4,5‐trimethoxyphenyl)quinolin‐4‐amine; 49) with a narrow‐spectrum kinome profile. These compounds may be useful tools to explore the therapeutic potential of GAK in prevention of a broad range of infectious and systemic diseases.
Targeting GAK: The availability of chemical probes with improved selectivity for cyclin G associated kinase (GAK) or a different spectrum of off‐targets would be useful in target validation studies. Herein we report the synthesis and characterization of 4‐anilinoquinolines and 4‐anilinoquinazolines as potent narrow‐spectrum GAK inhibitors. Several of these compounds have the potential for development into high‐quality chemical probes for the study of GAK biology. |
| doi_str_mv | 10.1002/cmdc.201700663 |
| format | Article |
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Targeting GAK: The availability of chemical probes with improved selectivity for cyclin G associated kinase (GAK) or a different spectrum of off‐targets would be useful in target validation studies. Herein we report the synthesis and characterization of 4‐anilinoquinolines and 4‐anilinoquinazolines as potent narrow‐spectrum GAK inhibitors. Several of these compounds have the potential for development into high‐quality chemical probes for the study of GAK biology.</description><identifier>ISSN: 1860-7179</identifier><identifier>EISSN: 1860-7187</identifier><identifier>DOI: 10.1002/cmdc.201700663</identifier><identifier>PMID: 29072804</identifier><language>eng</language><publisher>Germany</publisher><subject>Aniline Compounds - chemistry ; Aniline Compounds - metabolism ; Aniline Compounds - pharmacology ; anilinoquinolines ; antibacterial agents ; Binding Sites ; Catalytic Domain ; chemical probes ; cyclin G associated kinase (GAK) ; Drug Design ; Endocytosis - drug effects ; Humans ; Intracellular Signaling Peptides and Proteins - antagonists & inhibitors ; Intracellular Signaling Peptides and Proteins - metabolism ; Kinetics ; Molecular Docking Simulation ; Protein Binding ; Protein Kinase Inhibitors - chemistry ; Protein Kinase Inhibitors - metabolism ; Protein Kinase Inhibitors - pharmacology ; Protein Serine-Threonine Kinases - antagonists & inhibitors ; Protein Serine-Threonine Kinases - metabolism ; Quinazolines - chemistry ; Quinazolines - metabolism ; Quinazolines - pharmacology ; Structure-Activity Relationship ; Viruses - pathogenicity</subject><ispartof>ChemMedChem, 2018-01, Vol.13 (1), p.48-66</ispartof><rights>2018 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4213-37cb14f5febf03161ca2276ca8b2dd803f9b80930c69cc3d15eff989fcd893533</citedby><cites>FETCH-LOGICAL-c4213-37cb14f5febf03161ca2276ca8b2dd803f9b80930c69cc3d15eff989fcd893533</cites><orcidid>0000-0003-2858-8929 ; 0000-0001-5871-3458 ; 0000-0003-4181-8223 ; 0000-0002-9836-0068 ; 0000-0003-4799-6792</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcmdc.201700663$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcmdc.201700663$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29072804$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asquith, Christopher R. M.</creatorcontrib><creatorcontrib>Laitinen, Tuomo</creatorcontrib><creatorcontrib>Bennett, James M.</creatorcontrib><creatorcontrib>Godoi, Paulo H.</creatorcontrib><creatorcontrib>East, Michael P.</creatorcontrib><creatorcontrib>Tizzard, Graham J.</creatorcontrib><creatorcontrib>Graves, Lee M.</creatorcontrib><creatorcontrib>Johnson, Gary L.</creatorcontrib><creatorcontrib>Dornsife, Ronna E.</creatorcontrib><creatorcontrib>Wells, Carrow I.</creatorcontrib><creatorcontrib>Elkins, Jonathan M.</creatorcontrib><creatorcontrib>Willson, Timothy M.</creatorcontrib><creatorcontrib>Zuercher, William J.</creatorcontrib><title>Identification and Optimization of 4‐Anilinoquinolines as Inhibitors of Cyclin G Associated Kinase</title><title>ChemMedChem</title><addtitle>ChemMedChem</addtitle><description>4‐Anilinoquinolines were identified as potent and narrow‐spectrum inhibitors of the cyclin G associated kinase (GAK), an important regulator of viral and bacterial entry into host cells. Optimization of the 4‐anilino group and the 6,7‐quinoline substituents produced GAK inhibitors with nanomolar activity, over 50 000‐fold selectivity relative to other members of the numb‐associated kinase (NAK) subfamily, and a compound (6,7‐dimethoxy‐N‐(3,4,5‐trimethoxyphenyl)quinolin‐4‐amine; 49) with a narrow‐spectrum kinome profile. These compounds may be useful tools to explore the therapeutic potential of GAK in prevention of a broad range of infectious and systemic diseases.
Targeting GAK: The availability of chemical probes with improved selectivity for cyclin G associated kinase (GAK) or a different spectrum of off‐targets would be useful in target validation studies. Herein we report the synthesis and characterization of 4‐anilinoquinolines and 4‐anilinoquinazolines as potent narrow‐spectrum GAK inhibitors. 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M. ; Laitinen, Tuomo ; Bennett, James M. ; Godoi, Paulo H. ; East, Michael P. ; Tizzard, Graham J. ; Graves, Lee M. ; Johnson, Gary L. ; Dornsife, Ronna E. ; Wells, Carrow I. ; Elkins, Jonathan M. ; Willson, Timothy M. ; Zuercher, William J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4213-37cb14f5febf03161ca2276ca8b2dd803f9b80930c69cc3d15eff989fcd893533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aniline Compounds - chemistry</topic><topic>Aniline Compounds - metabolism</topic><topic>Aniline Compounds - pharmacology</topic><topic>anilinoquinolines</topic><topic>antibacterial agents</topic><topic>Binding Sites</topic><topic>Catalytic Domain</topic><topic>chemical probes</topic><topic>cyclin G associated kinase (GAK)</topic><topic>Drug Design</topic><topic>Endocytosis - drug effects</topic><topic>Humans</topic><topic>Intracellular Signaling Peptides and Proteins - antagonists & inhibitors</topic><topic>Intracellular Signaling Peptides and Proteins - metabolism</topic><topic>Kinetics</topic><topic>Molecular Docking Simulation</topic><topic>Protein Binding</topic><topic>Protein Kinase Inhibitors - chemistry</topic><topic>Protein Kinase Inhibitors - metabolism</topic><topic>Protein Kinase Inhibitors - pharmacology</topic><topic>Protein Serine-Threonine Kinases - antagonists & inhibitors</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>Quinazolines - chemistry</topic><topic>Quinazolines - metabolism</topic><topic>Quinazolines - pharmacology</topic><topic>Structure-Activity Relationship</topic><topic>Viruses - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Asquith, Christopher R. M.</creatorcontrib><creatorcontrib>Laitinen, Tuomo</creatorcontrib><creatorcontrib>Bennett, James M.</creatorcontrib><creatorcontrib>Godoi, Paulo H.</creatorcontrib><creatorcontrib>East, Michael P.</creatorcontrib><creatorcontrib>Tizzard, Graham J.</creatorcontrib><creatorcontrib>Graves, Lee M.</creatorcontrib><creatorcontrib>Johnson, Gary L.</creatorcontrib><creatorcontrib>Dornsife, Ronna E.</creatorcontrib><creatorcontrib>Wells, Carrow I.</creatorcontrib><creatorcontrib>Elkins, Jonathan M.</creatorcontrib><creatorcontrib>Willson, Timothy M.</creatorcontrib><creatorcontrib>Zuercher, William J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>ChemMedChem</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asquith, Christopher R. M.</au><au>Laitinen, Tuomo</au><au>Bennett, James M.</au><au>Godoi, Paulo H.</au><au>East, Michael P.</au><au>Tizzard, Graham J.</au><au>Graves, Lee M.</au><au>Johnson, Gary L.</au><au>Dornsife, Ronna E.</au><au>Wells, Carrow I.</au><au>Elkins, Jonathan M.</au><au>Willson, Timothy M.</au><au>Zuercher, William J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and Optimization of 4‐Anilinoquinolines as Inhibitors of Cyclin G Associated Kinase</atitle><jtitle>ChemMedChem</jtitle><addtitle>ChemMedChem</addtitle><date>2018-01-08</date><risdate>2018</risdate><volume>13</volume><issue>1</issue><spage>48</spage><epage>66</epage><pages>48-66</pages><issn>1860-7179</issn><eissn>1860-7187</eissn><abstract>4‐Anilinoquinolines were identified as potent and narrow‐spectrum inhibitors of the cyclin G associated kinase (GAK), an important regulator of viral and bacterial entry into host cells. Optimization of the 4‐anilino group and the 6,7‐quinoline substituents produced GAK inhibitors with nanomolar activity, over 50 000‐fold selectivity relative to other members of the numb‐associated kinase (NAK) subfamily, and a compound (6,7‐dimethoxy‐N‐(3,4,5‐trimethoxyphenyl)quinolin‐4‐amine; 49) with a narrow‐spectrum kinome profile. These compounds may be useful tools to explore the therapeutic potential of GAK in prevention of a broad range of infectious and systemic diseases.
Targeting GAK: The availability of chemical probes with improved selectivity for cyclin G associated kinase (GAK) or a different spectrum of off‐targets would be useful in target validation studies. Herein we report the synthesis and characterization of 4‐anilinoquinolines and 4‐anilinoquinazolines as potent narrow‐spectrum GAK inhibitors. Several of these compounds have the potential for development into high‐quality chemical probes for the study of GAK biology.</abstract><cop>Germany</cop><pmid>29072804</pmid><doi>10.1002/cmdc.201700663</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0003-2858-8929</orcidid><orcidid>https://orcid.org/0000-0001-5871-3458</orcidid><orcidid>https://orcid.org/0000-0003-4181-8223</orcidid><orcidid>https://orcid.org/0000-0002-9836-0068</orcidid><orcidid>https://orcid.org/0000-0003-4799-6792</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aniline Compounds - chemistry Aniline Compounds - metabolism Aniline Compounds - pharmacology anilinoquinolines antibacterial agents Binding Sites Catalytic Domain chemical probes cyclin G associated kinase (GAK) Drug Design Endocytosis - drug effects Humans Intracellular Signaling Peptides and Proteins - antagonists & inhibitors Intracellular Signaling Peptides and Proteins - metabolism Kinetics Molecular Docking Simulation Protein Binding Protein Kinase Inhibitors - chemistry Protein Kinase Inhibitors - metabolism Protein Kinase Inhibitors - pharmacology Protein Serine-Threonine Kinases - antagonists & inhibitors Protein Serine-Threonine Kinases - metabolism Quinazolines - chemistry Quinazolines - metabolism Quinazolines - pharmacology Structure-Activity Relationship Viruses - pathogenicity |
| title | Identification and Optimization of 4‐Anilinoquinolines as Inhibitors of Cyclin G Associated Kinase |
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