Purification of FANCD2 sub‐complexes
Summary Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal...
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| Veröffentlicht in: | British journal of haematology 2010-07, Vol.150 (1), p.88-92 |
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| container_title | British journal of haematology |
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| creator | Zhi, Gang Chen, Xiaoyong Newcomb, William Brown, Jay Semmes, Oliver J. Kupfer, Gary M. |
| description | Summary
Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes. |
| doi_str_mv | 10.1111/j.1365-2141.2010.08217.x |
| format | Article |
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Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.</description><identifier>ISSN: 0007-1048</identifier><identifier>EISSN: 1365-2141</identifier><identifier>DOI: 10.1111/j.1365-2141.2010.08217.x</identifier><identifier>PMID: 20456353</identifier><identifier>CODEN: BJHEAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Anemias. Hemoglobinopathies ; Biological and medical sciences ; chromatography purification ; Chromatography, Gel - methods ; Diseases of red blood cells ; FANCD2 ; Fanconi anaemia ; Fanconi Anemia - metabolism ; Fanconi Anemia Complementation Group D2 Protein - isolation & purification ; Fanconi Anemia Complementation Group D2 Protein - metabolism ; Fanconi Anemia Complementation Group D2 Protein - ultrastructure ; HeLa Cells ; Hematologic and hematopoietic diseases ; Humans ; Medical sciences ; Microscopy, Electron</subject><ispartof>British journal of haematology, 2010-07, Vol.150 (1), p.88-92</ispartof><rights>2010 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4337-1701b351f929f9935933ce2071635f68095689232a7e1ff72c1a414a7fa0a5a43</citedby><cites>FETCH-LOGICAL-c4337-1701b351f929f9935933ce2071635f68095689232a7e1ff72c1a414a7fa0a5a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2141.2010.08217.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2141.2010.08217.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22975020$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20456353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhi, Gang</creatorcontrib><creatorcontrib>Chen, Xiaoyong</creatorcontrib><creatorcontrib>Newcomb, William</creatorcontrib><creatorcontrib>Brown, Jay</creatorcontrib><creatorcontrib>Semmes, Oliver J.</creatorcontrib><creatorcontrib>Kupfer, Gary M.</creatorcontrib><title>Purification of FANCD2 sub‐complexes</title><title>British journal of haematology</title><addtitle>Br J Haematol</addtitle><description>Summary
Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.</description><subject>Anemias. Hemoglobinopathies</subject><subject>Biological and medical sciences</subject><subject>chromatography purification</subject><subject>Chromatography, Gel - methods</subject><subject>Diseases of red blood cells</subject><subject>FANCD2</subject><subject>Fanconi anaemia</subject><subject>Fanconi Anemia - metabolism</subject><subject>Fanconi Anemia Complementation Group D2 Protein - isolation & purification</subject><subject>Fanconi Anemia Complementation Group D2 Protein - metabolism</subject><subject>Fanconi Anemia Complementation Group D2 Protein - ultrastructure</subject><subject>HeLa Cells</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><issn>0007-1048</issn><issn>1365-2141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1OwzAQhS0EgvJzBdQNsGqYseM4XoBUyr8QsIC15RobXKVJiRtodxyBM3ISEloK7PDGlufNe08fIW2ECOuzP4iQJbxDMcaIQv0LKUURTZZIazFYJi0AEB2EOF0j6yEMAJABx1WyRiHmCeOsRXZvq9I7b_TYF3m7cO3T7nXvmLZD1f94ezfFcJTZiQ2bZMXpLNit-b1B7k9P7nrnnaubs4te96pjYsbqLAHYZxydpNJJybhkzFgKAus0l6QgeZJKyqgWFp0T1KCOMdbCadBcx2yDHM58R1V_aB-MzcelztSo9ENdTlWhvfo7yf2TeixeFJdIEyFqg725QVk8VzaM1dAHY7NM57aoghKMSclZArUynSlNWYRQWrdIQVANZTVQDUzVwFQNZfVFWU3q1e3fLReL31hrwc5coIPRmSt1bnz40VEpONCmw8FM9-ozO_13AXV0ed682CcQA5Zt</recordid><startdate>201007</startdate><enddate>201007</enddate><creator>Zhi, Gang</creator><creator>Chen, Xiaoyong</creator><creator>Newcomb, William</creator><creator>Brown, Jay</creator><creator>Semmes, Oliver J.</creator><creator>Kupfer, Gary M.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201007</creationdate><title>Purification of FANCD2 sub‐complexes</title><author>Zhi, Gang ; Chen, Xiaoyong ; Newcomb, William ; Brown, Jay ; Semmes, Oliver J. ; Kupfer, Gary M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4337-1701b351f929f9935933ce2071635f68095689232a7e1ff72c1a414a7fa0a5a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Anemias. Hemoglobinopathies</topic><topic>Biological and medical sciences</topic><topic>chromatography purification</topic><topic>Chromatography, Gel - methods</topic><topic>Diseases of red blood cells</topic><topic>FANCD2</topic><topic>Fanconi anaemia</topic><topic>Fanconi Anemia - metabolism</topic><topic>Fanconi Anemia Complementation Group D2 Protein - isolation & purification</topic><topic>Fanconi Anemia Complementation Group D2 Protein - metabolism</topic><topic>Fanconi Anemia Complementation Group D2 Protein - ultrastructure</topic><topic>HeLa Cells</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhi, Gang</creatorcontrib><creatorcontrib>Chen, Xiaoyong</creatorcontrib><creatorcontrib>Newcomb, William</creatorcontrib><creatorcontrib>Brown, Jay</creatorcontrib><creatorcontrib>Semmes, Oliver J.</creatorcontrib><creatorcontrib>Kupfer, Gary M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhi, Gang</au><au>Chen, Xiaoyong</au><au>Newcomb, William</au><au>Brown, Jay</au><au>Semmes, Oliver J.</au><au>Kupfer, Gary M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of FANCD2 sub‐complexes</atitle><jtitle>British journal of haematology</jtitle><addtitle>Br J Haematol</addtitle><date>2010-07</date><risdate>2010</risdate><volume>150</volume><issue>1</issue><spage>88</spage><epage>92</epage><pages>88-92</pages><issn>0007-1048</issn><eissn>1365-2141</eissn><coden>BJHEAL</coden><abstract>Summary
Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20456353</pmid><doi>10.1111/j.1365-2141.2010.08217.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anemias. Hemoglobinopathies Biological and medical sciences chromatography purification Chromatography, Gel - methods Diseases of red blood cells FANCD2 Fanconi anaemia Fanconi Anemia - metabolism Fanconi Anemia Complementation Group D2 Protein - isolation & purification Fanconi Anemia Complementation Group D2 Protein - metabolism Fanconi Anemia Complementation Group D2 Protein - ultrastructure HeLa Cells Hematologic and hematopoietic diseases Humans Medical sciences Microscopy, Electron |
| title | Purification of FANCD2 sub‐complexes |
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