Difference in virulence and composition of a cariogenic biofilm according to substratum direction
The aim of this study was to investigate the difference in composition and virulence of Streptococcus mutans biofilms according to substratum direction. S. mutans biofilms (46-h-old) were formed on three different saliva-coated hydroxyapatite (sHA) disc direction groups: downward (discs placed in th...
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| Veröffentlicht in: | Scientific reports 2018-04, Vol.8 (1), p.6244-8, Article 6244 |
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| description | The aim of this study was to investigate the difference in composition and virulence of
Streptococcus mutans
biofilms according to substratum direction.
S. mutans
biofilms (46-h-old) were formed on three different saliva-coated hydroxyapatite (sHA) disc direction groups: downward (discs placed in the direction of gravity), vertical (discs placed parallel to gravity direction), and upward (discs placed opposite to gravity). The 46-h-old biofilms on sHA discs in the upward direction showed the highest biofilm accumulation, colony forming unit (CFU) count, and extracellular polysaccharide (EPS) amount, followed by those in the vertical and downward directions. In the confocal laser scanning microscopy (CLSM) study, the biofilms in the upward direction also showed the highest bacterial count (live or dead cells) and EPS biovolume. Scanning electron microscopy (SEM) analysis confirmed the microbiological and biochemical results. In addition, biofilm density and acid production were higher in the upward direction than those in the other directions. Our findings suggest that substratum direction, which might be related to gravity, strongly influences the formation and virulence of cariogenic biofilms and subsequent initiation of dental caries. Collectively, the differences in the formation and virulence of cariogenic biofilms are related to the direction of tooth surface (occlusal surfaces of mandibular teeth > proximal surfaces > occlusal surfaces of maxillary teeth). |
| doi_str_mv | 10.1038/s41598-018-24626-2 |
| format | Article |
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Streptococcus mutans
biofilms according to substratum direction.
S. mutans
biofilms (46-h-old) were formed on three different saliva-coated hydroxyapatite (sHA) disc direction groups: downward (discs placed in the direction of gravity), vertical (discs placed parallel to gravity direction), and upward (discs placed opposite to gravity). The 46-h-old biofilms on sHA discs in the upward direction showed the highest biofilm accumulation, colony forming unit (CFU) count, and extracellular polysaccharide (EPS) amount, followed by those in the vertical and downward directions. In the confocal laser scanning microscopy (CLSM) study, the biofilms in the upward direction also showed the highest bacterial count (live or dead cells) and EPS biovolume. Scanning electron microscopy (SEM) analysis confirmed the microbiological and biochemical results. In addition, biofilm density and acid production were higher in the upward direction than those in the other directions. Our findings suggest that substratum direction, which might be related to gravity, strongly influences the formation and virulence of cariogenic biofilms and subsequent initiation of dental caries. Collectively, the differences in the formation and virulence of cariogenic biofilms are related to the direction of tooth surface (occlusal surfaces of mandibular teeth > proximal surfaces > occlusal surfaces of maxillary teeth).</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-24626-2</identifier><identifier>PMID: 29674703</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/19 ; 631/326/46 ; 692/699/3017/3018 ; 96/34 ; Acid production ; Biofilms ; Confocal microscopy ; Dental caries ; Humanities and Social Sciences ; Hydroxyapatite ; Mandible ; Microscopy ; multidisciplinary ; Saliva ; Scanning electron microscopy ; Science ; Science (multidisciplinary) ; Teeth ; Virulence</subject><ispartof>Scientific reports, 2018-04, Vol.8 (1), p.6244-8, Article 6244</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-5140c4076bd2e42905fe1ec8af69dcfebb1a5fee3ca56e8ae601bb670a7663b3</citedby><cites>FETCH-LOGICAL-c540t-5140c4076bd2e42905fe1ec8af69dcfebb1a5fee3ca56e8ae601bb670a7663b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908833/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908833/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29674703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dang, Minh-Huy</creatorcontrib><creatorcontrib>Jung, Ji-Eun</creatorcontrib><creatorcontrib>Choi, Hyeon-Mi</creatorcontrib><creatorcontrib>Jeon, Jae-Gyu</creatorcontrib><title>Difference in virulence and composition of a cariogenic biofilm according to substratum direction</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The aim of this study was to investigate the difference in composition and virulence of
Streptococcus mutans
biofilms according to substratum direction.
S. mutans
biofilms (46-h-old) were formed on three different saliva-coated hydroxyapatite (sHA) disc direction groups: downward (discs placed in the direction of gravity), vertical (discs placed parallel to gravity direction), and upward (discs placed opposite to gravity). The 46-h-old biofilms on sHA discs in the upward direction showed the highest biofilm accumulation, colony forming unit (CFU) count, and extracellular polysaccharide (EPS) amount, followed by those in the vertical and downward directions. In the confocal laser scanning microscopy (CLSM) study, the biofilms in the upward direction also showed the highest bacterial count (live or dead cells) and EPS biovolume. Scanning electron microscopy (SEM) analysis confirmed the microbiological and biochemical results. In addition, biofilm density and acid production were higher in the upward direction than those in the other directions. Our findings suggest that substratum direction, which might be related to gravity, strongly influences the formation and virulence of cariogenic biofilms and subsequent initiation of dental caries. Collectively, the differences in the formation and virulence of cariogenic biofilms are related to the direction of tooth surface (occlusal surfaces of mandibular teeth > proximal surfaces > occlusal surfaces of maxillary teeth).</description><subject>14/19</subject><subject>631/326/46</subject><subject>692/699/3017/3018</subject><subject>96/34</subject><subject>Acid production</subject><subject>Biofilms</subject><subject>Confocal microscopy</subject><subject>Dental caries</subject><subject>Humanities and Social Sciences</subject><subject>Hydroxyapatite</subject><subject>Mandible</subject><subject>Microscopy</subject><subject>multidisciplinary</subject><subject>Saliva</subject><subject>Scanning electron microscopy</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Teeth</subject><subject>Virulence</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUtv1TAQhS0EolXpH2CBLLFhExg_k2yQUHlKldh0b9nO-OIqsS92Uol_X9_eUgoLvPFjvnM8o0PISwZvGYjhXZVMjUMHbOi41Fx3_Ak55SBVxwXnTx-dT8h5rdfQluKjZONzcsJH3csexCmxH2MIWDB5pDHRm1i2-e5i00R9Xva5xjXmRHOglnpbYt5hip66mEOcF2q9z2WKaUfXTOvm6lrsui10igX9QfmCPAt2rnh-v5-Rq8-fri6-dpffv3y7-HDZeSVh7RST4CX02k0cJR9BBWToBxv0OPmAzjHbnlB4qzQOFjUw53QPttdaOHFG3h9t95tbcPKYWiOz2Ze42PLLZBvN35UUf5hdvjFqhGEQohm8uTco-eeGdTVLrB7n2SbMWzUc-DBqxaVq6Ot_0Ou8ldSmO1C96kEDNIofKV9yrQXDQzMMzCFDc8zQtAzNXYaGN9Grx2M8SH4n1gBxBGorpR2WP3__x_YW1nepwA</recordid><startdate>20180419</startdate><enddate>20180419</enddate><creator>Dang, Minh-Huy</creator><creator>Jung, Ji-Eun</creator><creator>Choi, Hyeon-Mi</creator><creator>Jeon, Jae-Gyu</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180419</creationdate><title>Difference in virulence and composition of a cariogenic biofilm according to substratum direction</title><author>Dang, Minh-Huy ; Jung, Ji-Eun ; Choi, Hyeon-Mi ; Jeon, Jae-Gyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-5140c4076bd2e42905fe1ec8af69dcfebb1a5fee3ca56e8ae601bb670a7663b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>14/19</topic><topic>631/326/46</topic><topic>692/699/3017/3018</topic><topic>96/34</topic><topic>Acid production</topic><topic>Biofilms</topic><topic>Confocal microscopy</topic><topic>Dental caries</topic><topic>Humanities and Social Sciences</topic><topic>Hydroxyapatite</topic><topic>Mandible</topic><topic>Microscopy</topic><topic>multidisciplinary</topic><topic>Saliva</topic><topic>Scanning electron microscopy</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Teeth</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dang, Minh-Huy</creatorcontrib><creatorcontrib>Jung, Ji-Eun</creatorcontrib><creatorcontrib>Choi, Hyeon-Mi</creatorcontrib><creatorcontrib>Jeon, Jae-Gyu</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dang, Minh-Huy</au><au>Jung, Ji-Eun</au><au>Choi, Hyeon-Mi</au><au>Jeon, Jae-Gyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Difference in virulence and composition of a cariogenic biofilm according to substratum direction</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-04-19</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>6244</spage><epage>8</epage><pages>6244-8</pages><artnum>6244</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The aim of this study was to investigate the difference in composition and virulence of
Streptococcus mutans
biofilms according to substratum direction.
S. mutans
biofilms (46-h-old) were formed on three different saliva-coated hydroxyapatite (sHA) disc direction groups: downward (discs placed in the direction of gravity), vertical (discs placed parallel to gravity direction), and upward (discs placed opposite to gravity). The 46-h-old biofilms on sHA discs in the upward direction showed the highest biofilm accumulation, colony forming unit (CFU) count, and extracellular polysaccharide (EPS) amount, followed by those in the vertical and downward directions. In the confocal laser scanning microscopy (CLSM) study, the biofilms in the upward direction also showed the highest bacterial count (live or dead cells) and EPS biovolume. Scanning electron microscopy (SEM) analysis confirmed the microbiological and biochemical results. In addition, biofilm density and acid production were higher in the upward direction than those in the other directions. Our findings suggest that substratum direction, which might be related to gravity, strongly influences the formation and virulence of cariogenic biofilms and subsequent initiation of dental caries. Collectively, the differences in the formation and virulence of cariogenic biofilms are related to the direction of tooth surface (occlusal surfaces of mandibular teeth > proximal surfaces > occlusal surfaces of maxillary teeth).</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29674703</pmid><doi>10.1038/s41598-018-24626-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 14/19 631/326/46 692/699/3017/3018 96/34 Acid production Biofilms Confocal microscopy Dental caries Humanities and Social Sciences Hydroxyapatite Mandible Microscopy multidisciplinary Saliva Scanning electron microscopy Science Science (multidisciplinary) Teeth Virulence |
| title | Difference in virulence and composition of a cariogenic biofilm according to substratum direction |
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