Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized d...

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Veröffentlicht in:Journal of Visualized Experiments 2018-01 (131)
Hauptverfasser: Casanellas, Ignasi, Lagunas, Anna, Tsintzou, Iro, Vida, Yolanda, Collado, Daniel, Pérez-Inestrosa, Ezequiel, Rodríguez-Pereira, Cristina, Magalhaes, Joana, Gorostiza, Pau, Andrades, José A., Becerra, José, Samitier, Josep
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container_issue 131
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container_title Journal of Visualized Experiments
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creator Casanellas, Ignasi
Lagunas, Anna
Tsintzou, Iro
Vida, Yolanda
Collado, Daniel
Pérez-Inestrosa, Ezequiel
Rodríguez-Pereira, Cristina
Magalhaes, Joana
Gorostiza, Pau
Andrades, José A.
Becerra, José
Samitier, Josep
description Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.
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subjects Adhesiveness
Animals
Bioengineering
Cell Adhesion
Chondrogenesis
Dendrimers - chemistry
Fibroblasts - cytology
Mesenchymal Stem Cells - cytology
Mice
Nanostructures - chemistry
NIH 3T3 Cells
Oligopeptides - chemistry
Surface Properties
title Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
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