Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized d...
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creator | Casanellas, Ignasi Lagunas, Anna Tsintzou, Iro Vida, Yolanda Collado, Daniel Pérez-Inestrosa, Ezequiel Rodríguez-Pereira, Cristina Magalhaes, Joana Gorostiza, Pau Andrades, José A. Becerra, José Samitier, Josep |
description | Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells. |
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Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. 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Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.</description><subject>Adhesiveness</subject><subject>Animals</subject><subject>Bioengineering</subject><subject>Cell Adhesion</subject><subject>Chondrogenesis</subject><subject>Dendrimers - chemistry</subject><subject>Fibroblasts - cytology</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mice</subject><subject>Nanostructures - chemistry</subject><subject>NIH 3T3 Cells</subject><subject>Oligopeptides - chemistry</subject><subject>Surface Properties</subject><issn>1940-087X</issn><issn>1940-087X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUEtLAzEQDqLYWvsHPEguHleTfWY9CGXrC6oetOBtyWYn3S3bpCRpoeCPN7VV6mmGme8x8yE0pOQ6ynJ6k6RRnB2hPs1jEhCWfR4f9D10Zu2ckDQkCTtFvTCP44iESR99jUHVpl2ACSpuocZTBWtQ-JUrveTOgVEWO40nWvCu2-BCK2d0h99XRnIBeFQ3YFvPAGtv8Qi_gGt0vWWMWwPC4aLR3kDPQLXCz6QEA8q13LVanaMTyTsLw30doOnD_UfxFEzeHp-L0SQQEQtdwCN_eBVVVZ7kImUsBwZZVFcsy0QoY0k5AM2pjyHmdZqJpJIpDUVYiZrTmEE0QHc73eWqWkAt_AGGd-XS_83NptS8Lf9vVNuUM70uk5ywNGVe4GonIIy21oD841JSbuMvf-L3uMtDoz_Ub94ecLEDzPUayrleGeUf37O_Adadi_o</recordid><startdate>20180120</startdate><enddate>20180120</enddate><creator>Casanellas, Ignasi</creator><creator>Lagunas, Anna</creator><creator>Tsintzou, Iro</creator><creator>Vida, Yolanda</creator><creator>Collado, Daniel</creator><creator>Pérez-Inestrosa, Ezequiel</creator><creator>Rodríguez-Pereira, Cristina</creator><creator>Magalhaes, Joana</creator><creator>Gorostiza, Pau</creator><creator>Andrades, José A.</creator><creator>Becerra, José</creator><creator>Samitier, Josep</creator><general>MyJove Corporation</general><scope>ALKRA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20180120</creationdate><title>Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation</title><author>Casanellas, Ignasi ; Lagunas, Anna ; Tsintzou, Iro ; Vida, Yolanda ; Collado, Daniel ; Pérez-Inestrosa, Ezequiel ; Rodríguez-Pereira, Cristina ; Magalhaes, Joana ; Gorostiza, Pau ; Andrades, José A. ; Becerra, José ; Samitier, Josep</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-a3062b3bb959c6889e8e73db877c2f4f1aee1913794ad67c5bf612c2bcda148e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adhesiveness</topic><topic>Animals</topic><topic>Bioengineering</topic><topic>Cell Adhesion</topic><topic>Chondrogenesis</topic><topic>Dendrimers - chemistry</topic><topic>Fibroblasts - cytology</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mice</topic><topic>Nanostructures - chemistry</topic><topic>NIH 3T3 Cells</topic><topic>Oligopeptides - chemistry</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casanellas, Ignasi</creatorcontrib><creatorcontrib>Lagunas, Anna</creatorcontrib><creatorcontrib>Tsintzou, Iro</creatorcontrib><creatorcontrib>Vida, Yolanda</creatorcontrib><creatorcontrib>Collado, Daniel</creatorcontrib><creatorcontrib>Pérez-Inestrosa, Ezequiel</creatorcontrib><creatorcontrib>Rodríguez-Pereira, Cristina</creatorcontrib><creatorcontrib>Magalhaes, Joana</creatorcontrib><creatorcontrib>Gorostiza, Pau</creatorcontrib><creatorcontrib>Andrades, José A.</creatorcontrib><creatorcontrib>Becerra, José</creatorcontrib><creatorcontrib>Samitier, Josep</creatorcontrib><collection>JoVE Journal: Bioengineering</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Visualized Experiments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Casanellas, Ignasi</au><au>Lagunas, Anna</au><au>Tsintzou, Iro</au><au>Vida, Yolanda</au><au>Collado, Daniel</au><au>Pérez-Inestrosa, Ezequiel</au><au>Rodríguez-Pereira, Cristina</au><au>Magalhaes, Joana</au><au>Gorostiza, Pau</au><au>Andrades, José A.</au><au>Becerra, José</au><au>Samitier, Josep</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation</atitle><jtitle>Journal of Visualized Experiments</jtitle><addtitle>J Vis Exp</addtitle><date>2018-01-20</date><risdate>2018</risdate><issue>131</issue><issn>1940-087X</issn><eissn>1940-087X</eissn><abstract>Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. 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subjects | Adhesiveness Animals Bioengineering Cell Adhesion Chondrogenesis Dendrimers - chemistry Fibroblasts - cytology Mesenchymal Stem Cells - cytology Mice Nanostructures - chemistry NIH 3T3 Cells Oligopeptides - chemistry Surface Properties |
title | Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation |
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