Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized d...
Gespeichert in:
| Veröffentlicht in: | Journal of Visualized Experiments 2018-01 (131) |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 131 |
| container_start_page | |
| container_title | Journal of Visualized Experiments |
| container_volume | |
| creator | Casanellas, Ignasi Lagunas, Anna Tsintzou, Iro Vida, Yolanda Collado, Daniel Pérez-Inestrosa, Ezequiel Rodríguez-Pereira, Cristina Magalhaes, Joana Gorostiza, Pau Andrades, José A. Becerra, José Samitier, Josep |
| description | Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells. |
| doi_str_mv | 10.3791/56347 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_223</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5908668</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>29443025</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-a3062b3bb959c6889e8e73db877c2f4f1aee1913794ad67c5bf612c2bcda148e3</originalsourceid><addsrcrecordid>eNpVUEtLAzEQDqLYWvsHPEguHleTfWY9CGXrC6oetOBtyWYn3S3bpCRpoeCPN7VV6mmGme8x8yE0pOQ6ynJ6k6RRnB2hPs1jEhCWfR4f9D10Zu2ckDQkCTtFvTCP44iESR99jUHVpl2ACSpuocZTBWtQ-JUrveTOgVEWO40nWvCu2-BCK2d0h99XRnIBeFQ3YFvPAGtv8Qi_gGt0vWWMWwPC4aLR3kDPQLXCz6QEA8q13LVanaMTyTsLw30doOnD_UfxFEzeHp-L0SQQEQtdwCN_eBVVVZ7kImUsBwZZVFcsy0QoY0k5AM2pjyHmdZqJpJIpDUVYiZrTmEE0QHc73eWqWkAt_AGGd-XS_83NptS8Lf9vVNuUM70uk5ywNGVe4GonIIy21oD841JSbuMvf-L3uMtDoz_Ub94ecLEDzPUayrleGeUf37O_Adadi_o</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation</title><source>Journal of Visualized Experiments : JoVE</source><creator>Casanellas, Ignasi ; Lagunas, Anna ; Tsintzou, Iro ; Vida, Yolanda ; Collado, Daniel ; Pérez-Inestrosa, Ezequiel ; Rodríguez-Pereira, Cristina ; Magalhaes, Joana ; Gorostiza, Pau ; Andrades, José A. ; Becerra, José ; Samitier, Josep</creator><creatorcontrib>Casanellas, Ignasi ; Lagunas, Anna ; Tsintzou, Iro ; Vida, Yolanda ; Collado, Daniel ; Pérez-Inestrosa, Ezequiel ; Rodríguez-Pereira, Cristina ; Magalhaes, Joana ; Gorostiza, Pau ; Andrades, José A. ; Becerra, José ; Samitier, Josep</creatorcontrib><description>Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.</description><identifier>ISSN: 1940-087X</identifier><identifier>EISSN: 1940-087X</identifier><identifier>DOI: 10.3791/56347</identifier><identifier>PMID: 29443025</identifier><language>eng</language><publisher>United States: MyJove Corporation</publisher><subject>Adhesiveness ; Animals ; Bioengineering ; Cell Adhesion ; Chondrogenesis ; Dendrimers - chemistry ; Fibroblasts - cytology ; Mesenchymal Stem Cells - cytology ; Mice ; Nanostructures - chemistry ; NIH 3T3 Cells ; Oligopeptides - chemistry ; Surface Properties</subject><ispartof>Journal of Visualized Experiments, 2018-01 (131)</ispartof><rights>Copyright © 2018, Journal of Visualized Experiments</rights><rights>Copyright © 2018, Journal of Visualized Experiments 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-a3062b3bb959c6889e8e73db877c2f4f1aee1913794ad67c5bf612c2bcda148e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttps://www.jove.com/files/email_thumbs/56347.png</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908668/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5908668/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,3845,27931,27932,53798,53800</link.rule.ids><linktorsrc>$$Uhttp://dx.doi.org/10.3791/56347$$EView_record_in_Journal_of_Visualized_Experiments$$FView_record_in_$$GJournal_of_Visualized_Experiments</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29443025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casanellas, Ignasi</creatorcontrib><creatorcontrib>Lagunas, Anna</creatorcontrib><creatorcontrib>Tsintzou, Iro</creatorcontrib><creatorcontrib>Vida, Yolanda</creatorcontrib><creatorcontrib>Collado, Daniel</creatorcontrib><creatorcontrib>Pérez-Inestrosa, Ezequiel</creatorcontrib><creatorcontrib>Rodríguez-Pereira, Cristina</creatorcontrib><creatorcontrib>Magalhaes, Joana</creatorcontrib><creatorcontrib>Gorostiza, Pau</creatorcontrib><creatorcontrib>Andrades, José A.</creatorcontrib><creatorcontrib>Becerra, José</creatorcontrib><creatorcontrib>Samitier, Josep</creatorcontrib><title>Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation</title><title>Journal of Visualized Experiments</title><addtitle>J Vis Exp</addtitle><description>Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.</description><subject>Adhesiveness</subject><subject>Animals</subject><subject>Bioengineering</subject><subject>Cell Adhesion</subject><subject>Chondrogenesis</subject><subject>Dendrimers - chemistry</subject><subject>Fibroblasts - cytology</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mice</subject><subject>Nanostructures - chemistry</subject><subject>NIH 3T3 Cells</subject><subject>Oligopeptides - chemistry</subject><subject>Surface Properties</subject><issn>1940-087X</issn><issn>1940-087X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUEtLAzEQDqLYWvsHPEguHleTfWY9CGXrC6oetOBtyWYn3S3bpCRpoeCPN7VV6mmGme8x8yE0pOQ6ynJ6k6RRnB2hPs1jEhCWfR4f9D10Zu2ckDQkCTtFvTCP44iESR99jUHVpl2ACSpuocZTBWtQ-JUrveTOgVEWO40nWvCu2-BCK2d0h99XRnIBeFQ3YFvPAGtv8Qi_gGt0vWWMWwPC4aLR3kDPQLXCz6QEA8q13LVanaMTyTsLw30doOnD_UfxFEzeHp-L0SQQEQtdwCN_eBVVVZ7kImUsBwZZVFcsy0QoY0k5AM2pjyHmdZqJpJIpDUVYiZrTmEE0QHc73eWqWkAt_AGGd-XS_83NptS8Lf9vVNuUM70uk5ywNGVe4GonIIy21oD841JSbuMvf-L3uMtDoz_Ub94ecLEDzPUayrleGeUf37O_Adadi_o</recordid><startdate>20180120</startdate><enddate>20180120</enddate><creator>Casanellas, Ignasi</creator><creator>Lagunas, Anna</creator><creator>Tsintzou, Iro</creator><creator>Vida, Yolanda</creator><creator>Collado, Daniel</creator><creator>Pérez-Inestrosa, Ezequiel</creator><creator>Rodríguez-Pereira, Cristina</creator><creator>Magalhaes, Joana</creator><creator>Gorostiza, Pau</creator><creator>Andrades, José A.</creator><creator>Becerra, José</creator><creator>Samitier, Josep</creator><general>MyJove Corporation</general><scope>ALKRA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20180120</creationdate><title>Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation</title><author>Casanellas, Ignasi ; Lagunas, Anna ; Tsintzou, Iro ; Vida, Yolanda ; Collado, Daniel ; Pérez-Inestrosa, Ezequiel ; Rodríguez-Pereira, Cristina ; Magalhaes, Joana ; Gorostiza, Pau ; Andrades, José A. ; Becerra, José ; Samitier, Josep</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-a3062b3bb959c6889e8e73db877c2f4f1aee1913794ad67c5bf612c2bcda148e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adhesiveness</topic><topic>Animals</topic><topic>Bioengineering</topic><topic>Cell Adhesion</topic><topic>Chondrogenesis</topic><topic>Dendrimers - chemistry</topic><topic>Fibroblasts - cytology</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mice</topic><topic>Nanostructures - chemistry</topic><topic>NIH 3T3 Cells</topic><topic>Oligopeptides - chemistry</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casanellas, Ignasi</creatorcontrib><creatorcontrib>Lagunas, Anna</creatorcontrib><creatorcontrib>Tsintzou, Iro</creatorcontrib><creatorcontrib>Vida, Yolanda</creatorcontrib><creatorcontrib>Collado, Daniel</creatorcontrib><creatorcontrib>Pérez-Inestrosa, Ezequiel</creatorcontrib><creatorcontrib>Rodríguez-Pereira, Cristina</creatorcontrib><creatorcontrib>Magalhaes, Joana</creatorcontrib><creatorcontrib>Gorostiza, Pau</creatorcontrib><creatorcontrib>Andrades, José A.</creatorcontrib><creatorcontrib>Becerra, José</creatorcontrib><creatorcontrib>Samitier, Josep</creatorcontrib><collection>JoVE Journal: Bioengineering</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Visualized Experiments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Casanellas, Ignasi</au><au>Lagunas, Anna</au><au>Tsintzou, Iro</au><au>Vida, Yolanda</au><au>Collado, Daniel</au><au>Pérez-Inestrosa, Ezequiel</au><au>Rodríguez-Pereira, Cristina</au><au>Magalhaes, Joana</au><au>Gorostiza, Pau</au><au>Andrades, José A.</au><au>Becerra, José</au><au>Samitier, Josep</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation</atitle><jtitle>Journal of Visualized Experiments</jtitle><addtitle>J Vis Exp</addtitle><date>2018-01-20</date><risdate>2018</risdate><issue>131</issue><issn>1940-087X</issn><eissn>1940-087X</eissn><abstract>Cellular adhesion and differentiation is conditioned by the nanoscale disposition of the extracellular matrix (ECM) components, with local concentrations having a major effect. Here we present a method to obtain large-scale uneven nanopatterns of arginine-glycine-aspartic acid (RGD)-functionalized dendrimers that permit the nanoscale control of local RGD surface density. Nanopatterns are formed by surface adsorption of dendrimers from solutions at different initial concentrations and are characterized by water contact angle (CA), X-ray photoelectron spectroscopy (XPS), and scanning probe microscopy techniques such as scanning tunneling microscopy (STM) and atomic force microscopy (AFM). The local surface density of RGD is measured using AFM images by means of probability contour maps of minimum interparticle distances and then correlated with cell adhesion response and differentiation. The nanopatterning method presented here is a simple procedure that can be scaled up in a straightforward manner to large surface areas. It is thus fully compatible with cell culture protocols and can be applied to other ligands that exert concentration-dependent effects on cells.</abstract><cop>United States</cop><pub>MyJove Corporation</pub><pmid>29443025</pmid><doi>10.3791/56347</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 1940-087X |
| ispartof | Journal of Visualized Experiments, 2018-01 (131) |
| issn | 1940-087X 1940-087X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5908668 |
| source | Journal of Visualized Experiments : JoVE |
| subjects | Adhesiveness Animals Bioengineering Cell Adhesion Chondrogenesis Dendrimers - chemistry Fibroblasts - cytology Mesenchymal Stem Cells - cytology Mice Nanostructures - chemistry NIH 3T3 Cells Oligopeptides - chemistry Surface Properties |
| title | Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-09T03%3A01%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_223&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dendrimer-based%20Uneven%20Nanopatterns%20to%20Locally%20Control%20Surface%20Adhesiveness:%20A%20Method%20to%20Direct%20Chondrogenic%20Differentiation&rft.jtitle=Journal%20of%20Visualized%20Experiments&rft.au=Casanellas,%20Ignasi&rft.date=2018-01-20&rft.issue=131&rft.issn=1940-087X&rft.eissn=1940-087X&rft_id=info:doi/10.3791/56347&rft_dat=%3Cpubmed_223%3E29443025%3C/pubmed_223%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/29443025&rfr_iscdi=true |