Fecal microbiota transplantation reverses antibiotic and chemotherapy-induced gut dysbiosis in mice
Fecal microbiota transplantation (FMT) is now widely used to treat recurrent Clostridium difficile infection, but has been less studied as a means to restore microbiome diversity and composition following antibiotic or chemotherapy treatments. The purpose of our study was to assess the efficacy of F...
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| Veröffentlicht in: | Scientific reports 2018-04, Vol.8 (1), p.6219-11, Article 6219 |
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| creator | Le Bastard, Quentin Ward, Tonya Sidiropoulos, Dimitri Hillmann, Benjamin M. Chun, Chan Lan Sadowsky, Michael J. Knights, Dan Montassier, Emmanuel |
| description | Fecal microbiota transplantation (FMT) is now widely used to treat recurrent
Clostridium difficile
infection, but has been less studied as a means to restore microbiome diversity and composition following antibiotic or chemotherapy treatments. The purpose of our study was to assess the efficacy of FMT to reverse antibiotic- and chemotherapy-induced gut dysbiosis in a mouse model. C57BL/6J mice were treated with ampicillin for 1 week and/or received a single intraperitoneal injection of 5-Fluorouracil. Fresh stool was collected and analyzed using shotgun metagenomics and the Illumina sequencing platform. Ampicillin caused a significant and immediate decrease in bacterial species richness and diversity that persisted for one week. In mice that received FMT, disruption of the intestinal microbiota was reversed immediately. Antibiotic and chemotherapy administration caused significant alteration in species distribution, including a decrease in the relative proportions of
Clostridium scindens
and
Faecalibacterium prausnitzii
, and an increase in known pathogenic species. In mice receiving FMT, we observed a significant increase in species known to exhibit anti-inflammatory properties. Moreover, chemotherapy led to a critical decrease in key ‘health-promoting’ species and to an altered functional profile, especially when chemotherapy was administered in tandem with antibiotics, and that FMT can ameliorate these effects. |
| doi_str_mv | 10.1038/s41598-018-24342-x |
| format | Article |
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Clostridium difficile
infection, but has been less studied as a means to restore microbiome diversity and composition following antibiotic or chemotherapy treatments. The purpose of our study was to assess the efficacy of FMT to reverse antibiotic- and chemotherapy-induced gut dysbiosis in a mouse model. C57BL/6J mice were treated with ampicillin for 1 week and/or received a single intraperitoneal injection of 5-Fluorouracil. Fresh stool was collected and analyzed using shotgun metagenomics and the Illumina sequencing platform. Ampicillin caused a significant and immediate decrease in bacterial species richness and diversity that persisted for one week. In mice that received FMT, disruption of the intestinal microbiota was reversed immediately. Antibiotic and chemotherapy administration caused significant alteration in species distribution, including a decrease in the relative proportions of
Clostridium scindens
and
Faecalibacterium prausnitzii
, and an increase in known pathogenic species. In mice receiving FMT, we observed a significant increase in species known to exhibit anti-inflammatory properties. Moreover, chemotherapy led to a critical decrease in key ‘health-promoting’ species and to an altered functional profile, especially when chemotherapy was administered in tandem with antibiotics, and that FMT can ameliorate these effects.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-24342-x</identifier><identifier>PMID: 29670191</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>45/23 ; 5-Fluorouracil ; 631/326/22/1290 ; 631/326/2565/2142 ; 631/67/70 ; 64/60 ; Ampicillin ; Anti-inflammatory agents ; Antibiotics ; Chemotherapy ; Digestive system ; Dysbacteriosis ; Fecal microflora ; Gastrointestinal tract ; Health promotion ; Humanities and Social Sciences ; Inflammation ; Intestinal microflora ; Intestine ; Microbiomes ; Microbiota ; multidisciplinary ; Recurrent infection ; Science ; Science (multidisciplinary) ; Species diversity ; Species richness ; Transplantation</subject><ispartof>Scientific reports, 2018-04, Vol.8 (1), p.6219-11, Article 6219</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-d099adf097cdc6f44be5557bbf148dadcb5a5b50fd2cc44602ada26b181089013</citedby><cites>FETCH-LOGICAL-c474t-d099adf097cdc6f44be5557bbf148dadcb5a5b50fd2cc44602ada26b181089013</cites><orcidid>0000-0002-9765-8663 ; 0000-0002-2313-1172 ; 0000-0001-8779-2781</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906603/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906603/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29670191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Le Bastard, Quentin</creatorcontrib><creatorcontrib>Ward, Tonya</creatorcontrib><creatorcontrib>Sidiropoulos, Dimitri</creatorcontrib><creatorcontrib>Hillmann, Benjamin M.</creatorcontrib><creatorcontrib>Chun, Chan Lan</creatorcontrib><creatorcontrib>Sadowsky, Michael J.</creatorcontrib><creatorcontrib>Knights, Dan</creatorcontrib><creatorcontrib>Montassier, Emmanuel</creatorcontrib><title>Fecal microbiota transplantation reverses antibiotic and chemotherapy-induced gut dysbiosis in mice</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Fecal microbiota transplantation (FMT) is now widely used to treat recurrent
Clostridium difficile
infection, but has been less studied as a means to restore microbiome diversity and composition following antibiotic or chemotherapy treatments. The purpose of our study was to assess the efficacy of FMT to reverse antibiotic- and chemotherapy-induced gut dysbiosis in a mouse model. C57BL/6J mice were treated with ampicillin for 1 week and/or received a single intraperitoneal injection of 5-Fluorouracil. Fresh stool was collected and analyzed using shotgun metagenomics and the Illumina sequencing platform. Ampicillin caused a significant and immediate decrease in bacterial species richness and diversity that persisted for one week. In mice that received FMT, disruption of the intestinal microbiota was reversed immediately. Antibiotic and chemotherapy administration caused significant alteration in species distribution, including a decrease in the relative proportions of
Clostridium scindens
and
Faecalibacterium prausnitzii
, and an increase in known pathogenic species. In mice receiving FMT, we observed a significant increase in species known to exhibit anti-inflammatory properties. 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reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-04-18</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>6219</spage><epage>11</epage><pages>6219-11</pages><artnum>6219</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Fecal microbiota transplantation (FMT) is now widely used to treat recurrent
Clostridium difficile
infection, but has been less studied as a means to restore microbiome diversity and composition following antibiotic or chemotherapy treatments. The purpose of our study was to assess the efficacy of FMT to reverse antibiotic- and chemotherapy-induced gut dysbiosis in a mouse model. C57BL/6J mice were treated with ampicillin for 1 week and/or received a single intraperitoneal injection of 5-Fluorouracil. Fresh stool was collected and analyzed using shotgun metagenomics and the Illumina sequencing platform. Ampicillin caused a significant and immediate decrease in bacterial species richness and diversity that persisted for one week. In mice that received FMT, disruption of the intestinal microbiota was reversed immediately. Antibiotic and chemotherapy administration caused significant alteration in species distribution, including a decrease in the relative proportions of
Clostridium scindens
and
Faecalibacterium prausnitzii
, and an increase in known pathogenic species. In mice receiving FMT, we observed a significant increase in species known to exhibit anti-inflammatory properties. Moreover, chemotherapy led to a critical decrease in key ‘health-promoting’ species and to an altered functional profile, especially when chemotherapy was administered in tandem with antibiotics, and that FMT can ameliorate these effects.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29670191</pmid><doi>10.1038/s41598-018-24342-x</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9765-8663</orcidid><orcidid>https://orcid.org/0000-0002-2313-1172</orcidid><orcidid>https://orcid.org/0000-0001-8779-2781</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 45/23 5-Fluorouracil 631/326/22/1290 631/326/2565/2142 631/67/70 64/60 Ampicillin Anti-inflammatory agents Antibiotics Chemotherapy Digestive system Dysbacteriosis Fecal microflora Gastrointestinal tract Health promotion Humanities and Social Sciences Inflammation Intestinal microflora Intestine Microbiomes Microbiota multidisciplinary Recurrent infection Science Science (multidisciplinary) Species diversity Species richness Transplantation |
| title | Fecal microbiota transplantation reverses antibiotic and chemotherapy-induced gut dysbiosis in mice |
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