Combined Therapy with Extracorporeal Shock Wave and Adipose-Derived Mesenchymal Stem Cells Remarkably Improved Acute Ischemia-Reperfusion Injury of Quadriceps Muscle

Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery ti...

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Veröffentlicht in:Oxidative medicine and cellular longevity 2018-01, Vol.2018 (2018), p.1-14
Hauptverfasser: Lin, Pao-Yuan, Chen, Chien-Chang, Chen, Chen-Yu, Shao, Pei-Lin, Yip, Hon-Kan, Chung, Wen-Jung, Hsueh, Shu-Kai, Chiang, John Y., Chen, Kuan-Hung, Sung, Pei-Hsun, Sheu, Jiunn-Jye, Wu, Re-Wen, Yin, Tsung-Cheng, Hsu, Shan-Ling
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Sprache:eng
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Zusammenfassung:Extracorporeal shock wave (ECSW) and adipose-derived mesenchymal stem cells (ADMSCs) have been recognized to have capacities of anti-inflammation and angiogenesis. We tested the hypothesis that ECSW and ADMSC therapy could attenuate ischemia-reperfusion- (IR-) induced thigh injury (femoral artery tightened for 6 h then the tightness was relieved) in rats. Adult male SD rats (n=30) were divided into group 1 (sham-control), group 2 (IR), group 3 (IR + ECSW/120 impulses at 0.12 mJ/mm2 given at 3 h/24 h/72 h after IR), group 4 (allogenic ADMSC/1.2 × 106 cell intramuscular and 1.2 × 106 cell intravenous injections 3 h after IR procedure), and group 5 (ECSW + ADMSC). At day 7 after the IR procedure, the left quadriceps muscle was harvested for studies. At 18 h after the IR procedure, serum myoglobin/creatine phosphokinase (CPK) levels were highest in group 2, lowest in group 1, and with intermediate values significantly progressively reduced in groups 3 to 5 (all p
ISSN:1942-0900
1942-0994
DOI:10.1155/2018/6012636