Transcript levels of the Aspergillus fumigatus Cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment
Abstract Aspergillus fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. During the first 8 hours of development A. fumigatus conidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner....
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Veröffentlicht in: | Medical mycology (Oxford) 2017-06, Vol.55 (4), p.445-452 |
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creator | Oda, Ken Bignell, Elaine Kang, S. Earl Momany, Michelle |
description | Abstract
Aspergillus fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. During the first 8 hours of development A. fumigatus conidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner. The transition from isotropic to polar growth is critical for tissue invasion and pathogenesis. In the current work, we used two-color microarrays to examine the A. fumigatus transcriptome during early development, focusing on the isotropic to polar switch. The most highly regulated transcripts in the isotropic to polar switch did not include known polarity genes. Transcripts encoding the Cdc42 module, polarisome components, and septins, known to be critical players in polarity, showed relatively steady levels during the isotropic to polar switch. Indeed, these transcripts were present in dormant conidia, and their levels changed little from dormancy through germ tube emergence. Not only did the isotropic to polar switch show little change in the expression of key polarity genes of the Cdc42 module, polarisome, and septins, it also showed the lowest overall levels of both up- and downregulation in early development. |
doi_str_mv | 10.1093/mmy/myw085 |
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Aspergillus fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. During the first 8 hours of development A. fumigatus conidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner. The transition from isotropic to polar growth is critical for tissue invasion and pathogenesis. In the current work, we used two-color microarrays to examine the A. fumigatus transcriptome during early development, focusing on the isotropic to polar switch. The most highly regulated transcripts in the isotropic to polar switch did not include known polarity genes. Transcripts encoding the Cdc42 module, polarisome components, and septins, known to be critical players in polarity, showed relatively steady levels during the isotropic to polar switch. Indeed, these transcripts were present in dormant conidia, and their levels changed little from dormancy through germ tube emergence. Not only did the isotropic to polar switch show little change in the expression of key polarity genes of the Cdc42 module, polarisome, and septins, it also showed the lowest overall levels of both up- and downregulation in early development.</description><identifier>ISSN: 1369-3786</identifier><identifier>EISSN: 1460-2709</identifier><identifier>DOI: 10.1093/mmy/myw085</identifier><identifier>PMID: 27664992</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Aspergillus fumigatus - genetics ; Aspergillus fumigatus - growth & development ; cdc42 GTP-Binding Protein - biosynthesis ; Gene Expression Profiling ; Microarray Analysis ; Multienzyme Complexes - analysis ; Multienzyme Complexes - genetics ; Original ; Septins - biosynthesis ; Septins - genetics</subject><ispartof>Medical mycology (Oxford), 2017-06, Vol.55 (4), p.445-452</ispartof><rights>The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2016</rights><rights>The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-44521b64c0c82de19ab96ca36fb34ed8f151c779c5bccc52ec4fe51488e218df3</citedby><cites>FETCH-LOGICAL-c372t-44521b64c0c82de19ab96ca36fb34ed8f151c779c5bccc52ec4fe51488e218df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27664992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oda, Ken</creatorcontrib><creatorcontrib>Bignell, Elaine</creatorcontrib><creatorcontrib>Kang, S. Earl</creatorcontrib><creatorcontrib>Momany, Michelle</creatorcontrib><title>Transcript levels of the Aspergillus fumigatus Cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment</title><title>Medical mycology (Oxford)</title><addtitle>Med Mycol</addtitle><description>Abstract
Aspergillus fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. During the first 8 hours of development A. fumigatus conidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner. The transition from isotropic to polar growth is critical for tissue invasion and pathogenesis. In the current work, we used two-color microarrays to examine the A. fumigatus transcriptome during early development, focusing on the isotropic to polar switch. The most highly regulated transcripts in the isotropic to polar switch did not include known polarity genes. Transcripts encoding the Cdc42 module, polarisome components, and septins, known to be critical players in polarity, showed relatively steady levels during the isotropic to polar switch. Indeed, these transcripts were present in dormant conidia, and their levels changed little from dormancy through germ tube emergence. Not only did the isotropic to polar switch show little change in the expression of key polarity genes of the Cdc42 module, polarisome, and septins, it also showed the lowest overall levels of both up- and downregulation in early development.</description><subject>Aspergillus fumigatus - genetics</subject><subject>Aspergillus fumigatus - growth & development</subject><subject>cdc42 GTP-Binding Protein - biosynthesis</subject><subject>Gene Expression Profiling</subject><subject>Microarray Analysis</subject><subject>Multienzyme Complexes - analysis</subject><subject>Multienzyme Complexes - genetics</subject><subject>Original</subject><subject>Septins - biosynthesis</subject><subject>Septins - genetics</subject><issn>1369-3786</issn><issn>1460-2709</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2K1jAUhosozji68QIkG0HEOkmaNslmYPjwDwbcjOuQpqdtJGlqks7Q-_CCjXyfg25cnRfOw3MS3qp6SfB7gmVz6f1-6fd7LNpH1TlhHa4px_JxyU0n64aL7qx6ltJ3jAmXtHlanVHedUxKel79vI16SSbaNSMHd-ASCiPKM6DrtEKcrHNbQuPm7aRzSYfBMIp8GDYH79AanI42BV-yXgaUYM12QRMskFCawz1yNmcHyMx6mQCNMXg0hOj1YnaUw0mQdwQp697ZNHtY8vPqyahdgheneVF9-_jh9vC5vvn66cvh-qY2Dae5ZqylpO-YwUbQAYjUveyMbrqxbxgMYiQtMZxL0_bGmJaCYSO0hAkBlIhhbC6qq6N33XoPgymno3ZqjdbruKugrfp3s9hZTeFOtUJ2grdF8OYkiOHHVv6gvE0GnNMLhC0pIlrJGcG8KejbI2piSCnC-HCGYPW7RlVqVMcaC_zq74c9oH96K8DrIxC29X-iXxYNrB0</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Oda, Ken</creator><creator>Bignell, Elaine</creator><creator>Kang, S. Earl</creator><creator>Momany, Michelle</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170601</creationdate><title>Transcript levels of the Aspergillus fumigatus Cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment</title><author>Oda, Ken ; Bignell, Elaine ; Kang, S. Earl ; Momany, Michelle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-44521b64c0c82de19ab96ca36fb34ed8f151c779c5bccc52ec4fe51488e218df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aspergillus fumigatus - genetics</topic><topic>Aspergillus fumigatus - growth & development</topic><topic>cdc42 GTP-Binding Protein - biosynthesis</topic><topic>Gene Expression Profiling</topic><topic>Microarray Analysis</topic><topic>Multienzyme Complexes - analysis</topic><topic>Multienzyme Complexes - genetics</topic><topic>Original</topic><topic>Septins - biosynthesis</topic><topic>Septins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oda, Ken</creatorcontrib><creatorcontrib>Bignell, Elaine</creatorcontrib><creatorcontrib>Kang, S. Earl</creatorcontrib><creatorcontrib>Momany, Michelle</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medical mycology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oda, Ken</au><au>Bignell, Elaine</au><au>Kang, S. Earl</au><au>Momany, Michelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcript levels of the Aspergillus fumigatus Cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment</atitle><jtitle>Medical mycology (Oxford)</jtitle><addtitle>Med Mycol</addtitle><date>2017-06-01</date><risdate>2017</risdate><volume>55</volume><issue>4</issue><spage>445</spage><epage>452</epage><pages>445-452</pages><issn>1369-3786</issn><eissn>1460-2709</eissn><abstract>Abstract
Aspergillus fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. During the first 8 hours of development A. fumigatus conidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner. The transition from isotropic to polar growth is critical for tissue invasion and pathogenesis. In the current work, we used two-color microarrays to examine the A. fumigatus transcriptome during early development, focusing on the isotropic to polar switch. The most highly regulated transcripts in the isotropic to polar switch did not include known polarity genes. Transcripts encoding the Cdc42 module, polarisome components, and septins, known to be critical players in polarity, showed relatively steady levels during the isotropic to polar switch. Indeed, these transcripts were present in dormant conidia, and their levels changed little from dormancy through germ tube emergence. Not only did the isotropic to polar switch show little change in the expression of key polarity genes of the Cdc42 module, polarisome, and septins, it also showed the lowest overall levels of both up- and downregulation in early development.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27664992</pmid><doi>10.1093/mmy/myw085</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
subjects | Aspergillus fumigatus - genetics Aspergillus fumigatus - growth & development cdc42 GTP-Binding Protein - biosynthesis Gene Expression Profiling Microarray Analysis Multienzyme Complexes - analysis Multienzyme Complexes - genetics Original Septins - biosynthesis Septins - genetics |
title | Transcript levels of the Aspergillus fumigatus Cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment |
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