Validation of a novel rat-holding device for studying heat- and mechanical-evoked trigeminal nocifensive behavioral responses
To test the reliability and validity of a novel rat-holding device designed to be used in conjunction with the plantar test apparatus for studying nocifensive behavioral responses in an established model of temporomandibular joint (TMJ) pathology. Thirty-five young adult male Sprague-Dawley rats wer...
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| Veröffentlicht in: | Journal of orofacial pain 2012-01, Vol.26 (4), p.337-344 |
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| container_title | Journal of orofacial pain |
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| creator | Garrett, Filip G Hawkins, Jordan L Overmyer, Allison E Hayden, Joshua B Durham, Paul L |
| description | To test the reliability and validity of a novel rat-holding device designed to be used in conjunction with the plantar test apparatus for studying nocifensive behavioral responses in an established model of temporomandibular joint (TMJ) pathology.
Thirty-five young adult male Sprague-Dawley rats were used. Withdrawal latencies in response to infrared 40 heat stimulation of the submandibular region in naïve animals (n = 4) and animals injected with saline or complete Freund's adjuvant (CFA) in the TMJ (n > 9) were measured over a 2-week time period. Nocifensive responses to mechanical stimulation of the cutaneous tissue directly over the TMJ with von Frey filaments were investigated in animals injected with CFA in the TMJ (n = 6). The effect on nocifensive responses to heat and mechanical stimulation of subcutaneous administration of buprenorphine (0.05 mg/kg) into the hindquarter was assessed in CFA and cotreated animals (n = 6). Statistical analysis was performed using a nonparametric Mann-Whitney U test.
Under basal conditions, withdrawal latencies to heat stimulation of the orofacial region remained consistently around 15 seconds over 14 days. Unilateral CFA injection in the TMJ significantly decreased heat-withdrawal latencies on days 1, 2, 7, and 14 in the ipsilateral side (P < .05), but not contralateral side, when compared with basal values. CFA also significantly decreased the nocifensive threshold to mechanical stimulation on days 1, 2, and 7 postinjection (P < .05). CFA-mediated changes in heat withdrawal and mechanical thresholds in the orofacial region were significantly suppressed by subcutaneous administration of buprenorphine into the hindquarter (P < .05).
Findings from this study provide evidence to validate the use of this holding device for studying nocifensive behaviors in the orofacial region of rats in response to heat or mechanical orofacial stimulation. |
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Thirty-five young adult male Sprague-Dawley rats were used. Withdrawal latencies in response to infrared 40 heat stimulation of the submandibular region in naïve animals (n = 4) and animals injected with saline or complete Freund's adjuvant (CFA) in the TMJ (n > 9) were measured over a 2-week time period. Nocifensive responses to mechanical stimulation of the cutaneous tissue directly over the TMJ with von Frey filaments were investigated in animals injected with CFA in the TMJ (n = 6). The effect on nocifensive responses to heat and mechanical stimulation of subcutaneous administration of buprenorphine (0.05 mg/kg) into the hindquarter was assessed in CFA and cotreated animals (n = 6). Statistical analysis was performed using a nonparametric Mann-Whitney U test.
Under basal conditions, withdrawal latencies to heat stimulation of the orofacial region remained consistently around 15 seconds over 14 days. Unilateral CFA injection in the TMJ significantly decreased heat-withdrawal latencies on days 1, 2, 7, and 14 in the ipsilateral side (P < .05), but not contralateral side, when compared with basal values. CFA also significantly decreased the nocifensive threshold to mechanical stimulation on days 1, 2, and 7 postinjection (P < .05). CFA-mediated changes in heat withdrawal and mechanical thresholds in the orofacial region were significantly suppressed by subcutaneous administration of buprenorphine into the hindquarter (P < .05).
Findings from this study provide evidence to validate the use of this holding device for studying nocifensive behaviors in the orofacial region of rats in response to heat or mechanical orofacial stimulation.</description><identifier>ISSN: 1064-6655</identifier><identifier>EISSN: 1945-3396</identifier><identifier>PMID: 23110274</identifier><language>eng</language><publisher>United States</publisher><subject>Analgesics, Opioid - pharmacology ; Animals ; Avoidance Learning ; Behavior, Animal ; Buprenorphine - pharmacology ; Facial Pain - physiopathology ; Facial Pain - psychology ; Freund's Adjuvant - pharmacology ; Head Movements ; Hot Temperature ; Inflammation - chemically induced ; Male ; Neck ; Physical Stimulation ; Rats ; Rats, Sprague-Dawley ; Reaction Time ; Restraint, Physical - instrumentation ; Temporomandibular Joint - drug effects ; Temporomandibular Joint - pathology ; Trigeminal Nerve - physiopathology</subject><ispartof>Journal of orofacial pain, 2012-01, Vol.26 (4), p.337-344</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23110274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garrett, Filip G</creatorcontrib><creatorcontrib>Hawkins, Jordan L</creatorcontrib><creatorcontrib>Overmyer, Allison E</creatorcontrib><creatorcontrib>Hayden, Joshua B</creatorcontrib><creatorcontrib>Durham, Paul L</creatorcontrib><title>Validation of a novel rat-holding device for studying heat- and mechanical-evoked trigeminal nocifensive behavioral responses</title><title>Journal of orofacial pain</title><addtitle>J Orofac Pain</addtitle><description>To test the reliability and validity of a novel rat-holding device designed to be used in conjunction with the plantar test apparatus for studying nocifensive behavioral responses in an established model of temporomandibular joint (TMJ) pathology.
Thirty-five young adult male Sprague-Dawley rats were used. Withdrawal latencies in response to infrared 40 heat stimulation of the submandibular region in naïve animals (n = 4) and animals injected with saline or complete Freund's adjuvant (CFA) in the TMJ (n > 9) were measured over a 2-week time period. Nocifensive responses to mechanical stimulation of the cutaneous tissue directly over the TMJ with von Frey filaments were investigated in animals injected with CFA in the TMJ (n = 6). The effect on nocifensive responses to heat and mechanical stimulation of subcutaneous administration of buprenorphine (0.05 mg/kg) into the hindquarter was assessed in CFA and cotreated animals (n = 6). Statistical analysis was performed using a nonparametric Mann-Whitney U test.
Under basal conditions, withdrawal latencies to heat stimulation of the orofacial region remained consistently around 15 seconds over 14 days. Unilateral CFA injection in the TMJ significantly decreased heat-withdrawal latencies on days 1, 2, 7, and 14 in the ipsilateral side (P < .05), but not contralateral side, when compared with basal values. CFA also significantly decreased the nocifensive threshold to mechanical stimulation on days 1, 2, and 7 postinjection (P < .05). CFA-mediated changes in heat withdrawal and mechanical thresholds in the orofacial region were significantly suppressed by subcutaneous administration of buprenorphine into the hindquarter (P < .05).
Findings from this study provide evidence to validate the use of this holding device for studying nocifensive behaviors in the orofacial region of rats in response to heat or mechanical orofacial stimulation.</description><subject>Analgesics, Opioid - pharmacology</subject><subject>Animals</subject><subject>Avoidance Learning</subject><subject>Behavior, Animal</subject><subject>Buprenorphine - pharmacology</subject><subject>Facial Pain - physiopathology</subject><subject>Facial Pain - psychology</subject><subject>Freund's Adjuvant - pharmacology</subject><subject>Head Movements</subject><subject>Hot Temperature</subject><subject>Inflammation - chemically induced</subject><subject>Male</subject><subject>Neck</subject><subject>Physical Stimulation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reaction Time</subject><subject>Restraint, Physical - instrumentation</subject><subject>Temporomandibular Joint - drug effects</subject><subject>Temporomandibular Joint - pathology</subject><subject>Trigeminal Nerve - physiopathology</subject><issn>1064-6655</issn><issn>1945-3396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkN1KxDAQhYso7rr6CpIXCCRpmiY3giz-wYI36m2ZNtM22ial6Rb2wne34g96NYdzznwMc5SsuZEZTVOjjhfNlKRKZdkqOYvxlTHBpVCnyUqknDORy3Xy_gKdszC54EmoCRAfZuzICBNtQ2edb4jF2VVI6jCSOO3t4dNrcSkQ8Jb0WLXgXQUdxTm8oSXT6BrsnYdugVWuRh_djKTEFmYXxsUeMQ7BR4znyUkNXcSL77lJnm9vnrb3dPd497C93tGBGzVRmee54SJTtUBUHNOSIdeqNCxFzUotZGlKrY2ySgAIwWSVM44IOtda1jbdJFdf3GFf9mgr9NNyRzGMrofxUARwxf_Eu7Zowlxk2kgt8gVw-Rfwu_nzyPQD7u5z_Q</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Garrett, Filip G</creator><creator>Hawkins, Jordan L</creator><creator>Overmyer, Allison E</creator><creator>Hayden, Joshua B</creator><creator>Durham, Paul L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>Validation of a novel rat-holding device for studying heat- and mechanical-evoked trigeminal nocifensive behavioral responses</title><author>Garrett, Filip G ; Hawkins, Jordan L ; Overmyer, Allison E ; Hayden, Joshua B ; Durham, Paul L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p196t-477791256f2ee61e3b0e186b903e80b824b9b8896d62aa2204c701eea87884fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analgesics, Opioid - pharmacology</topic><topic>Animals</topic><topic>Avoidance Learning</topic><topic>Behavior, Animal</topic><topic>Buprenorphine - pharmacology</topic><topic>Facial Pain - physiopathology</topic><topic>Facial Pain - psychology</topic><topic>Freund's Adjuvant - pharmacology</topic><topic>Head Movements</topic><topic>Hot Temperature</topic><topic>Inflammation - chemically induced</topic><topic>Male</topic><topic>Neck</topic><topic>Physical Stimulation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reaction Time</topic><topic>Restraint, Physical - instrumentation</topic><topic>Temporomandibular Joint - drug effects</topic><topic>Temporomandibular Joint - pathology</topic><topic>Trigeminal Nerve - physiopathology</topic><toplevel>online_resources</toplevel><creatorcontrib>Garrett, Filip G</creatorcontrib><creatorcontrib>Hawkins, Jordan L</creatorcontrib><creatorcontrib>Overmyer, Allison E</creatorcontrib><creatorcontrib>Hayden, Joshua B</creatorcontrib><creatorcontrib>Durham, Paul L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of orofacial pain</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garrett, Filip G</au><au>Hawkins, Jordan L</au><au>Overmyer, Allison E</au><au>Hayden, Joshua B</au><au>Durham, Paul L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation of a novel rat-holding device for studying heat- and mechanical-evoked trigeminal nocifensive behavioral responses</atitle><jtitle>Journal of orofacial pain</jtitle><addtitle>J Orofac Pain</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>26</volume><issue>4</issue><spage>337</spage><epage>344</epage><pages>337-344</pages><issn>1064-6655</issn><eissn>1945-3396</eissn><abstract>To test the reliability and validity of a novel rat-holding device designed to be used in conjunction with the plantar test apparatus for studying nocifensive behavioral responses in an established model of temporomandibular joint (TMJ) pathology.
Thirty-five young adult male Sprague-Dawley rats were used. Withdrawal latencies in response to infrared 40 heat stimulation of the submandibular region in naïve animals (n = 4) and animals injected with saline or complete Freund's adjuvant (CFA) in the TMJ (n > 9) were measured over a 2-week time period. Nocifensive responses to mechanical stimulation of the cutaneous tissue directly over the TMJ with von Frey filaments were investigated in animals injected with CFA in the TMJ (n = 6). The effect on nocifensive responses to heat and mechanical stimulation of subcutaneous administration of buprenorphine (0.05 mg/kg) into the hindquarter was assessed in CFA and cotreated animals (n = 6). Statistical analysis was performed using a nonparametric Mann-Whitney U test.
Under basal conditions, withdrawal latencies to heat stimulation of the orofacial region remained consistently around 15 seconds over 14 days. Unilateral CFA injection in the TMJ significantly decreased heat-withdrawal latencies on days 1, 2, 7, and 14 in the ipsilateral side (P < .05), but not contralateral side, when compared with basal values. CFA also significantly decreased the nocifensive threshold to mechanical stimulation on days 1, 2, and 7 postinjection (P < .05). CFA-mediated changes in heat withdrawal and mechanical thresholds in the orofacial region were significantly suppressed by subcutaneous administration of buprenorphine into the hindquarter (P < .05).
Findings from this study provide evidence to validate the use of this holding device for studying nocifensive behaviors in the orofacial region of rats in response to heat or mechanical orofacial stimulation.</abstract><cop>United States</cop><pmid>23110274</pmid><tpages>8</tpages></addata></record> |
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| subjects | Analgesics, Opioid - pharmacology Animals Avoidance Learning Behavior, Animal Buprenorphine - pharmacology Facial Pain - physiopathology Facial Pain - psychology Freund's Adjuvant - pharmacology Head Movements Hot Temperature Inflammation - chemically induced Male Neck Physical Stimulation Rats Rats, Sprague-Dawley Reaction Time Restraint, Physical - instrumentation Temporomandibular Joint - drug effects Temporomandibular Joint - pathology Trigeminal Nerve - physiopathology |
| title | Validation of a novel rat-holding device for studying heat- and mechanical-evoked trigeminal nocifensive behavioral responses |
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