Haploid embryonic stem cells can be enriched and maintained by simple filtration

Mammalian haploid embryonic stem cells (haESCs) serve as a powerful tool for genetic analyses at both the cellular and organismal levels. However, spontaneous diploidization of haESCs limits their use in these analyses. Addition of small molecules to the culture medium to control the cell cycle can...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 2018-04, Vol.293 (14), p.5230-5235
Hauptverfasser:	Qu, Chao, Yan, Meng, Yang, Suming, Wang, Lingbo, Yin, Qi, Liu, Yuan, Chen, Yeguang, Li, Jinsong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blastocyst - cytology cell biology Cell Culture Techniques - methods Cell Line Cell Size cell sorting Cells, Cultured CRISPR-Cas Systems CRISPR/Cas diploidization Diploidy embryo embryonic stem cell Embryonic Stem Cells - cytology Embryonic Stem Cells - physiology FACS filtration Filtration - methods Gene Editing Genetic Testing haploid embryonic stem cells Haploidy Methods and Resources Mice Mouse Embryonic Stem Cells - cytology Mouse Embryonic Stem Cells - physiology Oocytes - cytology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!