The spread of KPC-producing Enterobacteriaceae in Spain: WGS analysis of the emerging high-risk clones of Klebsiella pneumoniae ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3
We analysed the microbiological traits and population structure of KPC-producing Enterobacteriaceae isolates collected in Spain between 2012 and 2014. We also performed a comparative WGS analysis of the three major KPC-producing Klebsiella pneumoniae clones detected. Carbapenemase and ESBL genes wer...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2016-12, Vol.71 (12), p.3392-3399 |
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| creator | Oteo, Jesús Pérez-Vázquez, María Bautista, Verónica Ortega, Adriana Zamarrón, Pilar Saez, David Fernández-Romero, Sara Lara, Noelia Ramiro, Raquel Aracil, Belén Campos, José |
| description | We analysed the microbiological traits and population structure of KPC-producing Enterobacteriaceae isolates collected in Spain between 2012 and 2014. We also performed a comparative WGS analysis of the three major KPC-producing Klebsiella pneumoniae clones detected.
Carbapenemase and ESBL genes were sequenced. The Institut Pasteur MLST scheme was used. WGS data were used to construct phylogenetic trees, to identify the determinants of resistance and to de novo assemble the genome of one representative isolate of each of the three major K. pneumoniae clones.
Of the 2443 carbapenemase-producing Enterobacteriaceae isolates identified during the study period, 111 (4.5%) produced KPC. Of these, 81 (73.0%) were K. pneumoniae and 13 (11.7%) were Enterobacter cloacae. Three major epidemic clones of K. pneumoniae were identified: ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3. ST11/KPC-2 differed from ST101/KPC-2 and ST512/KPC-3 by 27 819 and 6924 SNPs, respectively. ST101/KPC-2 differed from ST512/KPC-3 by 28 345 SNPs. Nine acquired resistance genes were found in ST11/KPC-2, 11 in ST512/KPC-3 and 13 in ST101/KPC-2. ST101/KPC-2 had the highest number of virulence genes (20). An 11 bp deletion at the end of the mgrB sequence was the cause of colistin resistance in ST512/KPC-3.
KPC-producing Enterobacteriaceae are increasing in Spain. Most KPC-producing K. pneumoniae isolates belonged to only five clones: ST11 and ST512 caused interregional spread, ST101 caused regional spread and ST1961 and ST678 produced independent hospital outbreaks. ST101/KPC-2 had the highest number of resistance and virulence genes. ST101/KPC-2 and ST512/KPC-3 were recently implicated in the spread of KPC in Italy. |
| doi_str_mv | 10.1093/jac/dkw321 |
| format | Article |
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Carbapenemase and ESBL genes were sequenced. The Institut Pasteur MLST scheme was used. WGS data were used to construct phylogenetic trees, to identify the determinants of resistance and to de novo assemble the genome of one representative isolate of each of the three major K. pneumoniae clones.
Of the 2443 carbapenemase-producing Enterobacteriaceae isolates identified during the study period, 111 (4.5%) produced KPC. Of these, 81 (73.0%) were K. pneumoniae and 13 (11.7%) were Enterobacter cloacae. Three major epidemic clones of K. pneumoniae were identified: ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3. ST11/KPC-2 differed from ST101/KPC-2 and ST512/KPC-3 by 27 819 and 6924 SNPs, respectively. ST101/KPC-2 differed from ST512/KPC-3 by 28 345 SNPs. Nine acquired resistance genes were found in ST11/KPC-2, 11 in ST512/KPC-3 and 13 in ST101/KPC-2. ST101/KPC-2 had the highest number of virulence genes (20). An 11 bp deletion at the end of the mgrB sequence was the cause of colistin resistance in ST512/KPC-3.
KPC-producing Enterobacteriaceae are increasing in Spain. Most KPC-producing K. pneumoniae isolates belonged to only five clones: ST11 and ST512 caused interregional spread, ST101 caused regional spread and ST1961 and ST678 produced independent hospital outbreaks. ST101/KPC-2 had the highest number of resistance and virulence genes. ST101/KPC-2 and ST512/KPC-3 were recently implicated in the spread of KPC in Italy.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkw321</identifier><identifier>PMID: 27530752</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>beta-Lactamases - genetics ; Cross Infection - epidemiology ; Cross Infection - microbiology ; Editor's Choice ; Enterobacteriaceae - classification ; Enterobacteriaceae - enzymology ; Enterobacteriaceae - genetics ; Enterobacteriaceae - isolation & purification ; Enterobacteriaceae Infections - epidemiology ; Enterobacteriaceae Infections - microbiology ; Genome, Bacterial ; Genotype ; Humans ; Molecular Epidemiology ; Multilocus Sequence Typing ; Original Research ; Prevalence ; Sequence Analysis, DNA ; Spain - epidemiology</subject><ispartof>Journal of antimicrobial chemotherapy, 2016-12, Vol.71 (12), p.3392-3399</ispartof><rights>The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.</rights><rights>The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-67517e91a3b627a0a235ac727072c53cec258172a8380fe7b814355854c9fa9c3</citedby><cites>FETCH-LOGICAL-c342t-67517e91a3b627a0a235ac727072c53cec258172a8380fe7b814355854c9fa9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27530752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oteo, Jesús</creatorcontrib><creatorcontrib>Pérez-Vázquez, María</creatorcontrib><creatorcontrib>Bautista, Verónica</creatorcontrib><creatorcontrib>Ortega, Adriana</creatorcontrib><creatorcontrib>Zamarrón, Pilar</creatorcontrib><creatorcontrib>Saez, David</creatorcontrib><creatorcontrib>Fernández-Romero, Sara</creatorcontrib><creatorcontrib>Lara, Noelia</creatorcontrib><creatorcontrib>Ramiro, Raquel</creatorcontrib><creatorcontrib>Aracil, Belén</creatorcontrib><creatorcontrib>Campos, José</creatorcontrib><creatorcontrib>Spanish Antibiotic Resistance Surveillance Program Collaborating Group</creatorcontrib><title>The spread of KPC-producing Enterobacteriaceae in Spain: WGS analysis of the emerging high-risk clones of Klebsiella pneumoniae ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>We analysed the microbiological traits and population structure of KPC-producing Enterobacteriaceae isolates collected in Spain between 2012 and 2014. We also performed a comparative WGS analysis of the three major KPC-producing Klebsiella pneumoniae clones detected.
Carbapenemase and ESBL genes were sequenced. The Institut Pasteur MLST scheme was used. WGS data were used to construct phylogenetic trees, to identify the determinants of resistance and to de novo assemble the genome of one representative isolate of each of the three major K. pneumoniae clones.
Of the 2443 carbapenemase-producing Enterobacteriaceae isolates identified during the study period, 111 (4.5%) produced KPC. Of these, 81 (73.0%) were K. pneumoniae and 13 (11.7%) were Enterobacter cloacae. Three major epidemic clones of K. pneumoniae were identified: ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3. ST11/KPC-2 differed from ST101/KPC-2 and ST512/KPC-3 by 27 819 and 6924 SNPs, respectively. ST101/KPC-2 differed from ST512/KPC-3 by 28 345 SNPs. Nine acquired resistance genes were found in ST11/KPC-2, 11 in ST512/KPC-3 and 13 in ST101/KPC-2. ST101/KPC-2 had the highest number of virulence genes (20). An 11 bp deletion at the end of the mgrB sequence was the cause of colistin resistance in ST512/KPC-3.
KPC-producing Enterobacteriaceae are increasing in Spain. Most KPC-producing K. pneumoniae isolates belonged to only five clones: ST11 and ST512 caused interregional spread, ST101 caused regional spread and ST1961 and ST678 produced independent hospital outbreaks. ST101/KPC-2 had the highest number of resistance and virulence genes. ST101/KPC-2 and ST512/KPC-3 were recently implicated in the spread of KPC in Italy.</description><subject>beta-Lactamases - genetics</subject><subject>Cross Infection - epidemiology</subject><subject>Cross Infection - microbiology</subject><subject>Editor's Choice</subject><subject>Enterobacteriaceae - classification</subject><subject>Enterobacteriaceae - enzymology</subject><subject>Enterobacteriaceae - genetics</subject><subject>Enterobacteriaceae - isolation & purification</subject><subject>Enterobacteriaceae Infections - epidemiology</subject><subject>Enterobacteriaceae Infections - microbiology</subject><subject>Genome, Bacterial</subject><subject>Genotype</subject><subject>Humans</subject><subject>Molecular Epidemiology</subject><subject>Multilocus Sequence Typing</subject><subject>Original Research</subject><subject>Prevalence</subject><subject>Sequence Analysis, DNA</subject><subject>Spain - epidemiology</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkd1u1DAQhS0EosvCDQ-AfIkQYf0TxwkXSGhV2qqVQNpFXFqOM9l1m9ipnYD6RLwmTndblavxyGe-M_ZB6C0lnyip-Opam1Vz84cz-gwtaF6QjJGKPkcLwonIZC74CXoV4zUhpBBF-RKdMCk4kYIt0N_tHnAcAugG-xZf_lhnQ_DNZKzb4VM3QvC1NqlYbUADtg5vBm3dZ_zrbIO1091dtHEeHRMIegi7eXJvd_ss2HiDTecd3AsuO6ijha7TeHAw9d7ZBNxsKV3NtuzjfCbHJqGb1AvK7nv-Gr1odRfhzbEu0c9vp9v1eXb1_exi_fUqMzxnY1ZIQSVUVPO6YFITzbjQRjJJJDOCGzBMlFQyXfKStCDrkuZciFLkpmp1ZfgSfTlwh6nuoTHgxqA7NQTb63CnvLbq_xtn92rnfytRVul3ZQK8PwKCv50gjqq30cyvduCnqGiZU1kxmXyX6MNBaoKPMUD7aEOJmpNVKVl1SDaJ3z1d7FH6ECX_Byzvnbw</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Oteo, Jesús</creator><creator>Pérez-Vázquez, María</creator><creator>Bautista, Verónica</creator><creator>Ortega, Adriana</creator><creator>Zamarrón, Pilar</creator><creator>Saez, David</creator><creator>Fernández-Romero, Sara</creator><creator>Lara, Noelia</creator><creator>Ramiro, Raquel</creator><creator>Aracil, Belén</creator><creator>Campos, José</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161201</creationdate><title>The spread of KPC-producing Enterobacteriaceae in Spain: WGS analysis of the emerging high-risk clones of Klebsiella pneumoniae ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3</title><author>Oteo, Jesús ; Pérez-Vázquez, María ; Bautista, Verónica ; Ortega, Adriana ; Zamarrón, Pilar ; Saez, David ; Fernández-Romero, Sara ; Lara, Noelia ; Ramiro, Raquel ; Aracil, Belén ; Campos, José</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-67517e91a3b627a0a235ac727072c53cec258172a8380fe7b814355854c9fa9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>beta-Lactamases - genetics</topic><topic>Cross Infection - epidemiology</topic><topic>Cross Infection - microbiology</topic><topic>Editor's Choice</topic><topic>Enterobacteriaceae - classification</topic><topic>Enterobacteriaceae - enzymology</topic><topic>Enterobacteriaceae - genetics</topic><topic>Enterobacteriaceae - isolation & purification</topic><topic>Enterobacteriaceae Infections - epidemiology</topic><topic>Enterobacteriaceae Infections - microbiology</topic><topic>Genome, Bacterial</topic><topic>Genotype</topic><topic>Humans</topic><topic>Molecular Epidemiology</topic><topic>Multilocus Sequence Typing</topic><topic>Original Research</topic><topic>Prevalence</topic><topic>Sequence Analysis, DNA</topic><topic>Spain - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oteo, Jesús</creatorcontrib><creatorcontrib>Pérez-Vázquez, María</creatorcontrib><creatorcontrib>Bautista, Verónica</creatorcontrib><creatorcontrib>Ortega, Adriana</creatorcontrib><creatorcontrib>Zamarrón, Pilar</creatorcontrib><creatorcontrib>Saez, David</creatorcontrib><creatorcontrib>Fernández-Romero, Sara</creatorcontrib><creatorcontrib>Lara, Noelia</creatorcontrib><creatorcontrib>Ramiro, Raquel</creatorcontrib><creatorcontrib>Aracil, Belén</creatorcontrib><creatorcontrib>Campos, José</creatorcontrib><creatorcontrib>Spanish Antibiotic Resistance Surveillance Program Collaborating Group</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oteo, Jesús</au><au>Pérez-Vázquez, María</au><au>Bautista, Verónica</au><au>Ortega, Adriana</au><au>Zamarrón, Pilar</au><au>Saez, David</au><au>Fernández-Romero, Sara</au><au>Lara, Noelia</au><au>Ramiro, Raquel</au><au>Aracil, Belén</au><au>Campos, José</au><aucorp>Spanish Antibiotic Resistance Surveillance Program Collaborating Group</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The spread of KPC-producing Enterobacteriaceae in Spain: WGS analysis of the emerging high-risk clones of Klebsiella pneumoniae ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>71</volume><issue>12</issue><spage>3392</spage><epage>3399</epage><pages>3392-3399</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><abstract>We analysed the microbiological traits and population structure of KPC-producing Enterobacteriaceae isolates collected in Spain between 2012 and 2014. We also performed a comparative WGS analysis of the three major KPC-producing Klebsiella pneumoniae clones detected.
Carbapenemase and ESBL genes were sequenced. The Institut Pasteur MLST scheme was used. WGS data were used to construct phylogenetic trees, to identify the determinants of resistance and to de novo assemble the genome of one representative isolate of each of the three major K. pneumoniae clones.
Of the 2443 carbapenemase-producing Enterobacteriaceae isolates identified during the study period, 111 (4.5%) produced KPC. Of these, 81 (73.0%) were K. pneumoniae and 13 (11.7%) were Enterobacter cloacae. Three major epidemic clones of K. pneumoniae were identified: ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3. ST11/KPC-2 differed from ST101/KPC-2 and ST512/KPC-3 by 27 819 and 6924 SNPs, respectively. ST101/KPC-2 differed from ST512/KPC-3 by 28 345 SNPs. Nine acquired resistance genes were found in ST11/KPC-2, 11 in ST512/KPC-3 and 13 in ST101/KPC-2. ST101/KPC-2 had the highest number of virulence genes (20). An 11 bp deletion at the end of the mgrB sequence was the cause of colistin resistance in ST512/KPC-3.
KPC-producing Enterobacteriaceae are increasing in Spain. Most KPC-producing K. pneumoniae isolates belonged to only five clones: ST11 and ST512 caused interregional spread, ST101 caused regional spread and ST1961 and ST678 produced independent hospital outbreaks. ST101/KPC-2 had the highest number of resistance and virulence genes. ST101/KPC-2 and ST512/KPC-3 were recently implicated in the spread of KPC in Italy.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>27530752</pmid><doi>10.1093/jac/dkw321</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | beta-Lactamases - genetics Cross Infection - epidemiology Cross Infection - microbiology Editor's Choice Enterobacteriaceae - classification Enterobacteriaceae - enzymology Enterobacteriaceae - genetics Enterobacteriaceae - isolation & purification Enterobacteriaceae Infections - epidemiology Enterobacteriaceae Infections - microbiology Genome, Bacterial Genotype Humans Molecular Epidemiology Multilocus Sequence Typing Original Research Prevalence Sequence Analysis, DNA Spain - epidemiology |
| title | The spread of KPC-producing Enterobacteriaceae in Spain: WGS analysis of the emerging high-risk clones of Klebsiella pneumoniae ST11/KPC-2, ST101/KPC-2 and ST512/KPC-3 |
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