Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse
Members of the genus Neisseria have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer re...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2016-09, Vol.66 (9), p.3585-3593 |
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| creator | Weyand, Nathan J Ma, Mancheong Phifer-Rixey, Megan Taku, Nyiawung A Rendón, María A Hockenberry, Alyson M Kim, Won J Agellon, Al B Biais, Nicolas Suzuki, Taichi A Goodyer-Sait, Lily Harrison, Odile B Bratcher, Holly B Nachman, Michael W Maiden, Martin C J So, Magdalene |
| description | Members of the genus Neisseria have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus Neisseria from the oral cavity of the wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named Neisseria musculi sp. nov. (type strain AP2031T=DSM 101846T=CCUG 68283T=LMG 29261T), was studied further. Strain AP2031T/N. musculi grew well in vitro. It was naturally competent, taking up DNA in a DNA uptake sequence and pilT-dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of N. musculi sp. nov. make it an ideal candidate for use in developing a mouse model for studying Neisseria-host interactions. |
| doi_str_mv | 10.1099/ijsem.0.001237 |
| format | Article |
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By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus Neisseria from the oral cavity of the wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named Neisseria musculi sp. nov. (type strain AP2031T=DSM 101846T=CCUG 68283T=LMG 29261T), was studied further. Strain AP2031T/N. musculi grew well in vitro. It was naturally competent, taking up DNA in a DNA uptake sequence and pilT-dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of N. musculi sp. nov. make it an ideal candidate for use in developing a mouse model for studying Neisseria-host interactions.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.001237</identifier><identifier>PMID: 27298306</identifier><language>eng</language><publisher>England: Microbiology Society</publisher><subject>Animals ; Bacterial Typing Techniques ; Base Composition ; DNA, Bacterial - genetics ; Genes, Bacterial ; Mice - microbiology ; Mouth - microbiology ; Multilocus Sequence Typing ; Neisseria - classification ; Neisseria - genetics ; Neisseria - isolation & purification ; North America ; Nucleic Acid Hybridization ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA</subject><ispartof>International journal of systematic and evolutionary microbiology, 2016-09, Vol.66 (9), p.3585-3593</ispartof><rights>2016 IUMS 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-a3b304e2dcca075cfb538ee767027ffeb704b35ad18a0022ac234d9861bd31e93</citedby><cites>FETCH-LOGICAL-c390t-a3b304e2dcca075cfb538ee767027ffeb704b35ad18a0022ac234d9861bd31e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,3733,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27298306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weyand, Nathan J</creatorcontrib><creatorcontrib>Ma, Mancheong</creatorcontrib><creatorcontrib>Phifer-Rixey, Megan</creatorcontrib><creatorcontrib>Taku, Nyiawung A</creatorcontrib><creatorcontrib>Rendón, María A</creatorcontrib><creatorcontrib>Hockenberry, Alyson M</creatorcontrib><creatorcontrib>Kim, Won J</creatorcontrib><creatorcontrib>Agellon, Al B</creatorcontrib><creatorcontrib>Biais, Nicolas</creatorcontrib><creatorcontrib>Suzuki, Taichi A</creatorcontrib><creatorcontrib>Goodyer-Sait, Lily</creatorcontrib><creatorcontrib>Harrison, Odile B</creatorcontrib><creatorcontrib>Bratcher, Holly B</creatorcontrib><creatorcontrib>Nachman, Michael W</creatorcontrib><creatorcontrib>Maiden, Martin C J</creatorcontrib><creatorcontrib>So, Magdalene</creatorcontrib><title>Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Members of the genus Neisseria have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus Neisseria from the oral cavity of the wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named Neisseria musculi sp. nov. (type strain AP2031T=DSM 101846T=CCUG 68283T=LMG 29261T), was studied further. Strain AP2031T/N. musculi grew well in vitro. It was naturally competent, taking up DNA in a DNA uptake sequence and pilT-dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of N. musculi sp. nov. make it an ideal candidate for use in developing a mouse model for studying Neisseria-host interactions.</description><subject>Animals</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>DNA, Bacterial - genetics</subject><subject>Genes, Bacterial</subject><subject>Mice - microbiology</subject><subject>Mouth - microbiology</subject><subject>Multilocus Sequence Typing</subject><subject>Neisseria - classification</subject><subject>Neisseria - genetics</subject><subject>Neisseria - isolation & purification</subject><subject>North America</subject><subject>Nucleic Acid Hybridization</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUUtPwzAMjhCIjcGVI8qRAytO0ucFCU08JiG4gDhGaeqyTG0zknYIfj0dHRNcbMv-_PnxEXLKIGCQZZdm6bEOIABgXCR7ZMzCOJ5GIML9XczjETnyftljwhjgkIx4wrNUQDwmr3NvK9Ua21DVFFQvlFO6RWe-hqQt6SMa7_uMonXndVcZ6lcBbew6uKClszVtF0g_TFXQhe080npjj8lBqSqPJ1s_IS-3N8-z--nD0918dv0w1SKDdqpELiBEXmitIIl0mUciRUziBHhSlpgnEOYiUgVLFQDnSnMRFlkas7wQDDMxIVcD76rLayw0Nq1TlVw5Uyv3Ka0y8n-lMQv5ZtcySlOIkrAnON8SOPveoW9lbbzGqlIN9odIlvJ-GeCR6KHBANXOeu-w3I1hIDdqyB81JMhBjb7h7O9yO_jv-8U3GMWIVw</recordid><startdate>201609</startdate><enddate>201609</enddate><creator>Weyand, Nathan J</creator><creator>Ma, Mancheong</creator><creator>Phifer-Rixey, Megan</creator><creator>Taku, Nyiawung A</creator><creator>Rendón, María A</creator><creator>Hockenberry, Alyson M</creator><creator>Kim, Won J</creator><creator>Agellon, Al B</creator><creator>Biais, Nicolas</creator><creator>Suzuki, Taichi A</creator><creator>Goodyer-Sait, Lily</creator><creator>Harrison, Odile B</creator><creator>Bratcher, Holly B</creator><creator>Nachman, Michael W</creator><creator>Maiden, Martin C J</creator><creator>So, Magdalene</creator><general>Microbiology Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201609</creationdate><title>Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse</title><author>Weyand, Nathan J ; Ma, Mancheong ; Phifer-Rixey, Megan ; Taku, Nyiawung A ; Rendón, María A ; Hockenberry, Alyson M ; Kim, Won J ; Agellon, Al B ; Biais, Nicolas ; Suzuki, Taichi A ; Goodyer-Sait, Lily ; Harrison, Odile B ; Bratcher, Holly B ; Nachman, Michael W ; Maiden, Martin C J ; So, Magdalene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-a3b304e2dcca075cfb538ee767027ffeb704b35ad18a0022ac234d9861bd31e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>DNA, Bacterial - genetics</topic><topic>Genes, Bacterial</topic><topic>Mice - microbiology</topic><topic>Mouth - microbiology</topic><topic>Multilocus Sequence Typing</topic><topic>Neisseria - classification</topic><topic>Neisseria - genetics</topic><topic>Neisseria - isolation & purification</topic><topic>North America</topic><topic>Nucleic Acid Hybridization</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weyand, Nathan J</creatorcontrib><creatorcontrib>Ma, Mancheong</creatorcontrib><creatorcontrib>Phifer-Rixey, Megan</creatorcontrib><creatorcontrib>Taku, Nyiawung A</creatorcontrib><creatorcontrib>Rendón, María A</creatorcontrib><creatorcontrib>Hockenberry, Alyson M</creatorcontrib><creatorcontrib>Kim, Won J</creatorcontrib><creatorcontrib>Agellon, Al B</creatorcontrib><creatorcontrib>Biais, Nicolas</creatorcontrib><creatorcontrib>Suzuki, Taichi A</creatorcontrib><creatorcontrib>Goodyer-Sait, Lily</creatorcontrib><creatorcontrib>Harrison, Odile B</creatorcontrib><creatorcontrib>Bratcher, Holly B</creatorcontrib><creatorcontrib>Nachman, Michael W</creatorcontrib><creatorcontrib>Maiden, Martin C J</creatorcontrib><creatorcontrib>So, Magdalene</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weyand, Nathan J</au><au>Ma, Mancheong</au><au>Phifer-Rixey, Megan</au><au>Taku, Nyiawung A</au><au>Rendón, María A</au><au>Hockenberry, Alyson M</au><au>Kim, Won J</au><au>Agellon, Al B</au><au>Biais, Nicolas</au><au>Suzuki, Taichi A</au><au>Goodyer-Sait, Lily</au><au>Harrison, Odile B</au><au>Bratcher, Holly B</au><au>Nachman, Michael W</au><au>Maiden, Martin C J</au><au>So, Magdalene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2016-09</date><risdate>2016</risdate><volume>66</volume><issue>9</issue><spage>3585</spage><epage>3593</epage><pages>3585-3593</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Members of the genus Neisseria have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus Neisseria from the oral cavity of the wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named Neisseria musculi sp. nov. (type strain AP2031T=DSM 101846T=CCUG 68283T=LMG 29261T), was studied further. Strain AP2031T/N. musculi grew well in vitro. It was naturally competent, taking up DNA in a DNA uptake sequence and pilT-dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of N. musculi sp. nov. make it an ideal candidate for use in developing a mouse model for studying Neisseria-host interactions.</abstract><cop>England</cop><pub>Microbiology Society</pub><pmid>27298306</pmid><doi>10.1099/ijsem.0.001237</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | International journal of systematic and evolutionary microbiology, 2016-09, Vol.66 (9), p.3585-3593 |
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| language | eng |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Bacterial Typing Techniques Base Composition DNA, Bacterial - genetics Genes, Bacterial Mice - microbiology Mouth - microbiology Multilocus Sequence Typing Neisseria - classification Neisseria - genetics Neisseria - isolation & purification North America Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA |
| title | Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse |
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