Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion
Objectives This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Admin...
Gespeichert in:
| Veröffentlicht in: | Clinical oral investigations 2018-04, Vol.22 (3), p.1243-1252 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1252 |
|---|---|
| container_issue | 3 |
| container_start_page | 1243 |
| container_title | Clinical oral investigations |
| container_volume | 22 |
| creator | Palasuk, Jadesada Windsor, L. Jack Platt, Jeffrey A. Lvov, Yuri Geraldeli, Saulo Bottino, Marco C. |
| description | Objectives
This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration.
Materials and methods
Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated.
Results
Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity.
Conclusions
The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity.
Clinical significance
Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability. |
| doi_str_mv | 10.1007/s00784-017-2215-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5867196</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2017580214</sourcerecordid><originalsourceid>FETCH-LOGICAL-c562t-d30828977295e206f47e9a2d24c4136b826a8f0374a1bf93f53146ab1b7c9503</originalsourceid><addsrcrecordid>eNp1UctOwzAQtBCIlsIHcEGROBv8SOzkgoTKU2oFh4qr5cRO46q1S5xU5O9xSSlw4GKvvLOz4xkAzjG6wgjxax-ONIYIc0gITmB3AIY4pgxSzvHhV00gy1I8ACfeLxDCMeP0GAxImrGExHwI3u7cR1d0xdJYDZdOKq0iK61r2lzDlVOmNOFFqkp7s9E-MrYyuWmi6fQ11JGMlPMaKr3WVmnbRKX0lXH2FByVcun12e4egdnD_Wz8BCcvj8_j2wksEkYaqChKgxbOSZZoglgZc51JokhcxJiyPCVMpiWiPJY4LzNaJjR8QeY450WWIDoCNz3tus1XWhVBQS2XYl2blaw74aQRfzvWVGLuNiJJGccZCwSXO4LavbfaN2Lh2toGyYIEW5MUkWDiCOAeVdTO-1qX-w0YiW0Sok9ChBGxTUJ0Yebit7T9xLf1AUB6gA8tO9f1z-r_WT8BLueU5w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2017580214</pqid></control><display><type>article</type><title>Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Palasuk, Jadesada ; Windsor, L. Jack ; Platt, Jeffrey A. ; Lvov, Yuri ; Geraldeli, Saulo ; Bottino, Marco C.</creator><creatorcontrib>Palasuk, Jadesada ; Windsor, L. Jack ; Platt, Jeffrey A. ; Lvov, Yuri ; Geraldeli, Saulo ; Bottino, Marco C.</creatorcontrib><description>Objectives
This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration.
Materials and methods
Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated.
Results
Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity.
Conclusions
The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity.
Clinical significance
Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-017-2215-y</identifier><identifier>PMID: 28965247</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adhesives ; Anti-Bacterial Agents - chemistry ; Antibiotics ; Cell Culture Techniques ; Chromatography, High Pressure Liquid ; Cytotoxicity ; Dental caries ; Dentin ; Dentistry ; Doxycycline ; Doxycycline - chemistry ; Hardness ; Interstitial collagenase ; Materials Testing ; Matrix metalloproteinase ; Matrix Metalloproteinase 1 - drug effects ; Matrix Metalloproteinase Inhibitors - chemistry ; Medicine ; Metalloproteinase ; Nanotubes ; Nanotubes - chemistry ; Original Article ; Physicochemical properties ; Resin Cements - chemistry</subject><ispartof>Clinical oral investigations, 2018-04, Vol.22 (3), p.1243-1252</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>Clinical Oral Investigations is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-d30828977295e206f47e9a2d24c4136b826a8f0374a1bf93f53146ab1b7c9503</citedby><cites>FETCH-LOGICAL-c562t-d30828977295e206f47e9a2d24c4136b826a8f0374a1bf93f53146ab1b7c9503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-017-2215-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-017-2215-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28965247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palasuk, Jadesada</creatorcontrib><creatorcontrib>Windsor, L. Jack</creatorcontrib><creatorcontrib>Platt, Jeffrey A.</creatorcontrib><creatorcontrib>Lvov, Yuri</creatorcontrib><creatorcontrib>Geraldeli, Saulo</creatorcontrib><creatorcontrib>Bottino, Marco C.</creatorcontrib><title>Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration.
Materials and methods
Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated.
Results
Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity.
Conclusions
The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity.
Clinical significance
Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.</description><subject>Adhesives</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Antibiotics</subject><subject>Cell Culture Techniques</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cytotoxicity</subject><subject>Dental caries</subject><subject>Dentin</subject><subject>Dentistry</subject><subject>Doxycycline</subject><subject>Doxycycline - chemistry</subject><subject>Hardness</subject><subject>Interstitial collagenase</subject><subject>Materials Testing</subject><subject>Matrix metalloproteinase</subject><subject>Matrix Metalloproteinase 1 - drug effects</subject><subject>Matrix Metalloproteinase Inhibitors - chemistry</subject><subject>Medicine</subject><subject>Metalloproteinase</subject><subject>Nanotubes</subject><subject>Nanotubes - chemistry</subject><subject>Original Article</subject><subject>Physicochemical properties</subject><subject>Resin Cements - chemistry</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UctOwzAQtBCIlsIHcEGROBv8SOzkgoTKU2oFh4qr5cRO46q1S5xU5O9xSSlw4GKvvLOz4xkAzjG6wgjxax-ONIYIc0gITmB3AIY4pgxSzvHhV00gy1I8ACfeLxDCMeP0GAxImrGExHwI3u7cR1d0xdJYDZdOKq0iK61r2lzDlVOmNOFFqkp7s9E-MrYyuWmi6fQ11JGMlPMaKr3WVmnbRKX0lXH2FByVcun12e4egdnD_Wz8BCcvj8_j2wksEkYaqChKgxbOSZZoglgZc51JokhcxJiyPCVMpiWiPJY4LzNaJjR8QeY450WWIDoCNz3tus1XWhVBQS2XYl2blaw74aQRfzvWVGLuNiJJGccZCwSXO4LavbfaN2Lh2toGyYIEW5MUkWDiCOAeVdTO-1qX-w0YiW0Sok9ChBGxTUJ0Yebit7T9xLf1AUB6gA8tO9f1z-r_WT8BLueU5w</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Palasuk, Jadesada</creator><creator>Windsor, L. Jack</creator><creator>Platt, Jeffrey A.</creator><creator>Lvov, Yuri</creator><creator>Geraldeli, Saulo</creator><creator>Bottino, Marco C.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20180401</creationdate><title>Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion</title><author>Palasuk, Jadesada ; Windsor, L. Jack ; Platt, Jeffrey A. ; Lvov, Yuri ; Geraldeli, Saulo ; Bottino, Marco C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-d30828977295e206f47e9a2d24c4136b826a8f0374a1bf93f53146ab1b7c9503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adhesives</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Antibiotics</topic><topic>Cell Culture Techniques</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cytotoxicity</topic><topic>Dental caries</topic><topic>Dentin</topic><topic>Dentistry</topic><topic>Doxycycline</topic><topic>Doxycycline - chemistry</topic><topic>Hardness</topic><topic>Interstitial collagenase</topic><topic>Materials Testing</topic><topic>Matrix metalloproteinase</topic><topic>Matrix Metalloproteinase 1 - drug effects</topic><topic>Matrix Metalloproteinase Inhibitors - chemistry</topic><topic>Medicine</topic><topic>Metalloproteinase</topic><topic>Nanotubes</topic><topic>Nanotubes - chemistry</topic><topic>Original Article</topic><topic>Physicochemical properties</topic><topic>Resin Cements - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palasuk, Jadesada</creatorcontrib><creatorcontrib>Windsor, L. Jack</creatorcontrib><creatorcontrib>Platt, Jeffrey A.</creatorcontrib><creatorcontrib>Lvov, Yuri</creatorcontrib><creatorcontrib>Geraldeli, Saulo</creatorcontrib><creatorcontrib>Bottino, Marco C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palasuk, Jadesada</au><au>Windsor, L. Jack</au><au>Platt, Jeffrey A.</au><au>Lvov, Yuri</au><au>Geraldeli, Saulo</au><au>Bottino, Marco C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>22</volume><issue>3</issue><spage>1243</spage><epage>1252</epage><pages>1243-1252</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration.
Materials and methods
Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated.
Results
Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly, it did inhibit MMP-1 activity.
Conclusions
The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity.
Clinical significance
Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28965247</pmid><doi>10.1007/s00784-017-2215-y</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1432-6981 |
| ispartof | Clinical oral investigations, 2018-04, Vol.22 (3), p.1243-1252 |
| issn | 1432-6981 1436-3771 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5867196 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Adhesives Anti-Bacterial Agents - chemistry Antibiotics Cell Culture Techniques Chromatography, High Pressure Liquid Cytotoxicity Dental caries Dentin Dentistry Doxycycline Doxycycline - chemistry Hardness Interstitial collagenase Materials Testing Matrix metalloproteinase Matrix Metalloproteinase 1 - drug effects Matrix Metalloproteinase Inhibitors - chemistry Medicine Metalloproteinase Nanotubes Nanotubes - chemistry Original Article Physicochemical properties Resin Cements - chemistry |
| title | Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T18%3A20%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Doxycycline-loaded%20nanotube-modified%20adhesives%20inhibit%20MMP%20in%20a%20dose-dependent%20fashion&rft.jtitle=Clinical%20oral%20investigations&rft.au=Palasuk,%20Jadesada&rft.date=2018-04-01&rft.volume=22&rft.issue=3&rft.spage=1243&rft.epage=1252&rft.pages=1243-1252&rft.issn=1432-6981&rft.eissn=1436-3771&rft_id=info:doi/10.1007/s00784-017-2215-y&rft_dat=%3Cproquest_pubme%3E2017580214%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2017580214&rft_id=info:pmid/28965247&rfr_iscdi=true |