The Babesia divergens Asia Lineage Is Maintained through Enzootic Cycles between Ixodes persulcatus and Sika Deer in Hokkaido, Japan
Parasites of the Asia lineage, which are closely related to in Europe and sp. strain MO1 in the United States, were recently reported in sika deer ( ) in eastern Japan. To identify the tick vector(s) for this parasite, we conducted a field survey in Hokkaido, Japan, where the infection rate in sika...
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| creator | Zamoto-Niikura, Aya Tsuji, Masayoshi Qiang, Wei Morikawa, Shigeru Hanaki, Ken-Ichi Holman, Patricia J Ishihara, Chiaki |
| description | Parasites of the
Asia lineage, which are closely related to
in Europe and
sp. strain MO1 in the United States, were recently reported in sika deer (
) in eastern Japan. To identify the tick vector(s) for this parasite, we conducted a field survey in Hokkaido, Japan, where the infection rate in sika deer is the highest in the country. A specific PCR system which detects and discriminates between lineages within
and between those lineages and
showed that
(11/822), but not sympatric
(0/595) or
sp. (0/163) ticks, carried
Asia lineage. Genomic DNA was archived from salivary glands of partially engorged
females and three isolates of
Asia lineage were newly described. The 18S rRNA gene sequence of the isolates formed the Asia lineage cluster with those previously described in sika deer isolates. One salivary gland also contained parasites of
U.S. lineage, which were subsequently isolated in a hamster
(strain Etb5) was also detected in one
tick. The 18S rRNA sequence of Etb5 was 99.7% identical to that of
(AY046575) and was phylogenetically positioned in a taxon composed of
isolates from Europe, China, and Russia. The geographical distribution of
is consistent with that of
in sika deer in Japan. These results suggest that
is a principal vector for
in Japan and Eurasia, where
is predominantly distributed.
The
Asia lineage of parasites closely related to
in Europe and
sp. MO1 in the United States was recently reported in
in eastern Japan. In this study, specific PCR for the Asia lineage identified 11 positives in 822 host-seeking
ticks, a principal vector for many tick-borne disease agents. Gene sequences of three isolates obtained from DNA in salivary glands of female ticks were identical to each other and to those in
We also demonstrate the coinfection of
Asia lineage with
U.S. lineage in a tick salivary gland and, furthermore, isolated the latter in a hamster. These results suggest that
is the principal vector for
as well as for
, and both parasites may be occasionally cotransmitted by
This report will be important for public health, since infection may occur through transfusion. |
| doi_str_mv | 10.1128/AEM.02491-17 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5861815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2073514825</sourcerecordid><originalsourceid>FETCH-LOGICAL-c478t-d8bcd057928aea213a73f115d01098c03dab7c8a1757e2432614c6936e2932da3</originalsourceid><addsrcrecordid>eNpdkc1vEzEQxS0EoqFw44wsceHQLf7YXXsvSCEEGpSKA-VsTexJ4mZjB3u3tD3zh7NLSwUcRqOnefppnh4hLzk75Vzot9P5-SkTZcMLrh6RCWeNLiop68dkwljTFEKU7Ig8y_mSMVayWj8lR6KRqmQln5CfF1uk72GF2QN1_grTBkOm01EufUDYIF1keg4-dMOgo902xX6zpfNwG2PnLZ3d2BYzXWH3AzHQxXV0gzxgyn1roeszheDoV78D-gExUR_oWdztwLt4Qj_DAcJz8mQNbcYX9_uYfPs4v5idFcsvnxaz6bKwpdJd4fTKOlapRmhAEFyCkmvOK8fG0JZJBytlNXBVKRSlFDUvbd3IGoe8woE8Ju_uuId-tUdnMXQJWnNIfg_pxkTw5t9L8FuziVem0jXXvBoAb-4BKX7vMXdm77PFtoWAsc-GN41gXNeVGqyv_7Nexj6FIZ4RTMmKl1qMwJM7l00x54Trh2c4M2O9ZqjX_K7X8BH66u8AD-Y_fcpfLpKgYA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2073514825</pqid></control><display><type>article</type><title>The Babesia divergens Asia Lineage Is Maintained through Enzootic Cycles between Ixodes persulcatus and Sika Deer in Hokkaido, Japan</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Zamoto-Niikura, Aya ; Tsuji, Masayoshi ; Qiang, Wei ; Morikawa, Shigeru ; Hanaki, Ken-Ichi ; Holman, Patricia J ; Ishihara, Chiaki</creator><creatorcontrib>Zamoto-Niikura, Aya ; Tsuji, Masayoshi ; Qiang, Wei ; Morikawa, Shigeru ; Hanaki, Ken-Ichi ; Holman, Patricia J ; Ishihara, Chiaki</creatorcontrib><description>Parasites of the
Asia lineage, which are closely related to
in Europe and
sp. strain MO1 in the United States, were recently reported in sika deer (
) in eastern Japan. To identify the tick vector(s) for this parasite, we conducted a field survey in Hokkaido, Japan, where the infection rate in sika deer is the highest in the country. A specific PCR system which detects and discriminates between lineages within
and between those lineages and
showed that
(11/822), but not sympatric
(0/595) or
sp. (0/163) ticks, carried
Asia lineage. Genomic DNA was archived from salivary glands of partially engorged
females and three isolates of
Asia lineage were newly described. The 18S rRNA gene sequence of the isolates formed the Asia lineage cluster with those previously described in sika deer isolates. One salivary gland also contained parasites of
U.S. lineage, which were subsequently isolated in a hamster
(strain Etb5) was also detected in one
tick. The 18S rRNA sequence of Etb5 was 99.7% identical to that of
(AY046575) and was phylogenetically positioned in a taxon composed of
isolates from Europe, China, and Russia. The geographical distribution of
is consistent with that of
in sika deer in Japan. These results suggest that
is a principal vector for
in Japan and Eurasia, where
is predominantly distributed.
The
Asia lineage of parasites closely related to
in Europe and
sp. MO1 in the United States was recently reported in
in eastern Japan. In this study, specific PCR for the Asia lineage identified 11 positives in 822 host-seeking
ticks, a principal vector for many tick-borne disease agents. Gene sequences of three isolates obtained from DNA in salivary glands of female ticks were identical to each other and to those in
We also demonstrate the coinfection of
Asia lineage with
U.S. lineage in a tick salivary gland and, furthermore, isolated the latter in a hamster. These results suggest that
is the principal vector for
as well as for
, and both parasites may be occasionally cotransmitted by
This report will be important for public health, since infection may occur through transfusion.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.02491-17</identifier><identifier>PMID: 29374041</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Animals ; Babesia - genetics ; Babesia - physiology ; Babesia divergens ; Babesiosis - parasitology ; Babesiosis - transmission ; Base Sequence ; Body fluids ; Cervus nippon ; Deer ; Deoxyribonucleic acid ; DNA ; DNA, Protozoan - analysis ; Ectoparasites ; Field study ; Genomics ; Geographical distribution ; Haemaphysalis ; Host-Parasite Interactions ; Invertebrate Microbiology ; Ixodes - parasitology ; Ixodes persulcatus ; Japan ; Parasites ; Phylogeny ; RNA, Ribosomal, 18S - analysis ; rRNA 18S ; Salivary gland ; Salivary glands ; Sympatric populations ; Ticks</subject><ispartof>Applied and environmental microbiology, 2018-04, Vol.84 (7), p.e02491-17</ispartof><rights>Copyright © 2018 American Society for Microbiology.</rights><rights>Copyright American Society for Microbiology Apr 1, 2018</rights><rights>Copyright © 2018 American Society for Microbiology. 2018 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-d8bcd057928aea213a73f115d01098c03dab7c8a1757e2432614c6936e2932da3</citedby><cites>FETCH-LOGICAL-c478t-d8bcd057928aea213a73f115d01098c03dab7c8a1757e2432614c6936e2932da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861815/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5861815/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29374041$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zamoto-Niikura, Aya</creatorcontrib><creatorcontrib>Tsuji, Masayoshi</creatorcontrib><creatorcontrib>Qiang, Wei</creatorcontrib><creatorcontrib>Morikawa, Shigeru</creatorcontrib><creatorcontrib>Hanaki, Ken-Ichi</creatorcontrib><creatorcontrib>Holman, Patricia J</creatorcontrib><creatorcontrib>Ishihara, Chiaki</creatorcontrib><title>The Babesia divergens Asia Lineage Is Maintained through Enzootic Cycles between Ixodes persulcatus and Sika Deer in Hokkaido, Japan</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Parasites of the
Asia lineage, which are closely related to
in Europe and
sp. strain MO1 in the United States, were recently reported in sika deer (
) in eastern Japan. To identify the tick vector(s) for this parasite, we conducted a field survey in Hokkaido, Japan, where the infection rate in sika deer is the highest in the country. A specific PCR system which detects and discriminates between lineages within
and between those lineages and
showed that
(11/822), but not sympatric
(0/595) or
sp. (0/163) ticks, carried
Asia lineage. Genomic DNA was archived from salivary glands of partially engorged
females and three isolates of
Asia lineage were newly described. The 18S rRNA gene sequence of the isolates formed the Asia lineage cluster with those previously described in sika deer isolates. One salivary gland also contained parasites of
U.S. lineage, which were subsequently isolated in a hamster
(strain Etb5) was also detected in one
tick. The 18S rRNA sequence of Etb5 was 99.7% identical to that of
(AY046575) and was phylogenetically positioned in a taxon composed of
isolates from Europe, China, and Russia. The geographical distribution of
is consistent with that of
in sika deer in Japan. These results suggest that
is a principal vector for
in Japan and Eurasia, where
is predominantly distributed.
The
Asia lineage of parasites closely related to
in Europe and
sp. MO1 in the United States was recently reported in
in eastern Japan. In this study, specific PCR for the Asia lineage identified 11 positives in 822 host-seeking
ticks, a principal vector for many tick-borne disease agents. Gene sequences of three isolates obtained from DNA in salivary glands of female ticks were identical to each other and to those in
We also demonstrate the coinfection of
Asia lineage with
U.S. lineage in a tick salivary gland and, furthermore, isolated the latter in a hamster. These results suggest that
is the principal vector for
as well as for
, and both parasites may be occasionally cotransmitted by
This report will be important for public health, since infection may occur through transfusion.</description><subject>Animals</subject><subject>Babesia - genetics</subject><subject>Babesia - physiology</subject><subject>Babesia divergens</subject><subject>Babesiosis - parasitology</subject><subject>Babesiosis - transmission</subject><subject>Base Sequence</subject><subject>Body fluids</subject><subject>Cervus nippon</subject><subject>Deer</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Protozoan - analysis</subject><subject>Ectoparasites</subject><subject>Field study</subject><subject>Genomics</subject><subject>Geographical distribution</subject><subject>Haemaphysalis</subject><subject>Host-Parasite Interactions</subject><subject>Invertebrate Microbiology</subject><subject>Ixodes - parasitology</subject><subject>Ixodes persulcatus</subject><subject>Japan</subject><subject>Parasites</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 18S - analysis</subject><subject>rRNA 18S</subject><subject>Salivary gland</subject><subject>Salivary glands</subject><subject>Sympatric populations</subject><subject>Ticks</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1vEzEQxS0EoqFw44wsceHQLf7YXXsvSCEEGpSKA-VsTexJ4mZjB3u3tD3zh7NLSwUcRqOnefppnh4hLzk75Vzot9P5-SkTZcMLrh6RCWeNLiop68dkwljTFEKU7Ig8y_mSMVayWj8lR6KRqmQln5CfF1uk72GF2QN1_grTBkOm01EufUDYIF1keg4-dMOgo902xX6zpfNwG2PnLZ3d2BYzXWH3AzHQxXV0gzxgyn1roeszheDoV78D-gExUR_oWdztwLt4Qj_DAcJz8mQNbcYX9_uYfPs4v5idFcsvnxaz6bKwpdJd4fTKOlapRmhAEFyCkmvOK8fG0JZJBytlNXBVKRSlFDUvbd3IGoe8woE8Ju_uuId-tUdnMXQJWnNIfg_pxkTw5t9L8FuziVem0jXXvBoAb-4BKX7vMXdm77PFtoWAsc-GN41gXNeVGqyv_7Nexj6FIZ4RTMmKl1qMwJM7l00x54Trh2c4M2O9ZqjX_K7X8BH66u8AD-Y_fcpfLpKgYA</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Zamoto-Niikura, Aya</creator><creator>Tsuji, Masayoshi</creator><creator>Qiang, Wei</creator><creator>Morikawa, Shigeru</creator><creator>Hanaki, Ken-Ichi</creator><creator>Holman, Patricia J</creator><creator>Ishihara, Chiaki</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180401</creationdate><title>The Babesia divergens Asia Lineage Is Maintained through Enzootic Cycles between Ixodes persulcatus and Sika Deer in Hokkaido, Japan</title><author>Zamoto-Niikura, Aya ; Tsuji, Masayoshi ; Qiang, Wei ; Morikawa, Shigeru ; Hanaki, Ken-Ichi ; Holman, Patricia J ; Ishihara, Chiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-d8bcd057928aea213a73f115d01098c03dab7c8a1757e2432614c6936e2932da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Babesia - genetics</topic><topic>Babesia - physiology</topic><topic>Babesia divergens</topic><topic>Babesiosis - parasitology</topic><topic>Babesiosis - transmission</topic><topic>Base Sequence</topic><topic>Body fluids</topic><topic>Cervus nippon</topic><topic>Deer</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Protozoan - analysis</topic><topic>Ectoparasites</topic><topic>Field study</topic><topic>Genomics</topic><topic>Geographical distribution</topic><topic>Haemaphysalis</topic><topic>Host-Parasite Interactions</topic><topic>Invertebrate Microbiology</topic><topic>Ixodes - parasitology</topic><topic>Ixodes persulcatus</topic><topic>Japan</topic><topic>Parasites</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 18S - analysis</topic><topic>rRNA 18S</topic><topic>Salivary gland</topic><topic>Salivary glands</topic><topic>Sympatric populations</topic><topic>Ticks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zamoto-Niikura, Aya</creatorcontrib><creatorcontrib>Tsuji, Masayoshi</creatorcontrib><creatorcontrib>Qiang, Wei</creatorcontrib><creatorcontrib>Morikawa, Shigeru</creatorcontrib><creatorcontrib>Hanaki, Ken-Ichi</creatorcontrib><creatorcontrib>Holman, Patricia J</creatorcontrib><creatorcontrib>Ishihara, Chiaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zamoto-Niikura, Aya</au><au>Tsuji, Masayoshi</au><au>Qiang, Wei</au><au>Morikawa, Shigeru</au><au>Hanaki, Ken-Ichi</au><au>Holman, Patricia J</au><au>Ishihara, Chiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Babesia divergens Asia Lineage Is Maintained through Enzootic Cycles between Ixodes persulcatus and Sika Deer in Hokkaido, Japan</atitle><jtitle>Applied and environmental microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>84</volume><issue>7</issue><spage>e02491-17</spage><pages>e02491-17-</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><abstract>Parasites of the
Asia lineage, which are closely related to
in Europe and
sp. strain MO1 in the United States, were recently reported in sika deer (
) in eastern Japan. To identify the tick vector(s) for this parasite, we conducted a field survey in Hokkaido, Japan, where the infection rate in sika deer is the highest in the country. A specific PCR system which detects and discriminates between lineages within
and between those lineages and
showed that
(11/822), but not sympatric
(0/595) or
sp. (0/163) ticks, carried
Asia lineage. Genomic DNA was archived from salivary glands of partially engorged
females and three isolates of
Asia lineage were newly described. The 18S rRNA gene sequence of the isolates formed the Asia lineage cluster with those previously described in sika deer isolates. One salivary gland also contained parasites of
U.S. lineage, which were subsequently isolated in a hamster
(strain Etb5) was also detected in one
tick. The 18S rRNA sequence of Etb5 was 99.7% identical to that of
(AY046575) and was phylogenetically positioned in a taxon composed of
isolates from Europe, China, and Russia. The geographical distribution of
is consistent with that of
in sika deer in Japan. These results suggest that
is a principal vector for
in Japan and Eurasia, where
is predominantly distributed.
The
Asia lineage of parasites closely related to
in Europe and
sp. MO1 in the United States was recently reported in
in eastern Japan. In this study, specific PCR for the Asia lineage identified 11 positives in 822 host-seeking
ticks, a principal vector for many tick-borne disease agents. Gene sequences of three isolates obtained from DNA in salivary glands of female ticks were identical to each other and to those in
We also demonstrate the coinfection of
Asia lineage with
U.S. lineage in a tick salivary gland and, furthermore, isolated the latter in a hamster. These results suggest that
is the principal vector for
as well as for
, and both parasites may be occasionally cotransmitted by
This report will be important for public health, since infection may occur through transfusion.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>29374041</pmid><doi>10.1128/AEM.02491-17</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and environmental microbiology, 2018-04, Vol.84 (7), p.e02491-17 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5861815 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Babesia - genetics Babesia - physiology Babesia divergens Babesiosis - parasitology Babesiosis - transmission Base Sequence Body fluids Cervus nippon Deer Deoxyribonucleic acid DNA DNA, Protozoan - analysis Ectoparasites Field study Genomics Geographical distribution Haemaphysalis Host-Parasite Interactions Invertebrate Microbiology Ixodes - parasitology Ixodes persulcatus Japan Parasites Phylogeny RNA, Ribosomal, 18S - analysis rRNA 18S Salivary gland Salivary glands Sympatric populations Ticks |
| title | The Babesia divergens Asia Lineage Is Maintained through Enzootic Cycles between Ixodes persulcatus and Sika Deer in Hokkaido, Japan |
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