Extracted metabolite from Streptomyces Levis ABRIINW111 altered the gene expression in colon cancer

Extracted metabolite from Streptomyces Levis ABRIINW111 altered the gene expression in colon cancer

In this study we attempt to indicate anti-carcinogenic influence of ether extracted metabolites of sp. on gene expression in colon cancer. Colon cancer is one of the most prevalent cancers worldwide. In recent decades, researchers have been seeking the treatment for cancer. Natural products are valu...

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Veröffentlicht in:Gastroenterology and hepatology from bed to bench 2018-01, Vol.11 (1), p.34-41
Hauptverfasser: Fatourachi, Parisa, Faramarziyan Azimi Maragheh, Behnaz, Mohammadi, Seyede Momeneh, Valipour, Behnaz, Dehnad, Alireza, Nozad Charoudeh, Hojjatollah
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container_end_page 41
container_issue 1
container_start_page 34
container_title Gastroenterology and hepatology from bed to bench
container_volume 11
creator Fatourachi, Parisa
Faramarziyan Azimi Maragheh, Behnaz
Mohammadi, Seyede Momeneh
Valipour, Behnaz
Dehnad, Alireza
Nozad Charoudeh, Hojjatollah
description In this study we attempt to indicate anti-carcinogenic influence of ether extracted metabolites of sp. on gene expression in colon cancer. Colon cancer is one of the most prevalent cancers worldwide. In recent decades, researchers have been seeking the treatment for cancer. Natural products are valuable compounds with fewer side effects in comparison to chemotherapy drugs. Secondary metabolites were extracted with the inoculation of bacterial sample in Mueller Hinton Broth. MTT assay was done to evaluate the cytotoxicity effect of metabolites on SW480 cells. qRT-PCR was performed to observe effects of metabolites on Bcl-2, P53, SOX2, KLF4, β-Catenin, SMAD4, K-ras, BRAF genes expression in colon cancer. The metabolites exhibited cytotoxic effects on colon cancer in a dose/time dependent manner (P < 0.001). After 48 h treatment, fold expression of Bcl-2, SOX2, β-catenin, K-ras, BRAF genes fold of expression were decreased, whereas P53, KLF4, SMAD4 genes were increased in treated cells (P < 0.001). These findings indicate that ether extracted metabolites of ABRIINW111 have anti-carcinogenic effects on colon cancer.
doi_str_mv 10.22037/ghfbb.v0i0.1091
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title Extracted metabolite from Streptomyces Levis ABRIINW111 altered the gene expression in colon cancer
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