GATA1 Is a Sensitive and Specific Nuclear Marker for Erythroid and Megakaryocytic Lineages
Abstract Objectives: GATA binding factor 1 (GATA1) is a transcription factor essential for erythromegakaryocytic differentiation. Given its function in lineage specification, we sought to evaluate the immunohistochemical profile of GATA1 in normal marrow and acute leukemia and assess the use of GATA...
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| Veröffentlicht in: | American journal of clinical pathology 2017-04, Vol.147 (4), p.420-426 |
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| creator | Lee, Winston Y. Weinberg, Olga K. Pinkus, Geraldine S. |
| description | Abstract
Objectives: GATA binding factor 1 (GATA1) is a transcription factor essential for erythromegakaryocytic differentiation. Given its function in lineage specification, we sought to evaluate the immunohistochemical profile of GATA1 in normal marrow and acute leukemia and assess the use of GATA1 as a specific erythromegakaryocytic immunohistochemical marker.
Methods: Immunohistochemical studies for GATA1 expression were performed on bone marrow biopsy specimens to define its role in the evaluation of acute leukemia and other hematologic disorders.
Results: In normal marrows, intense nuclear reactivity is seen in immature erythroid precursors and megakaryocytes. Weak to moderate nuclear positivity is seen in eosinophils and mast cells. In marrows involved by acute leukemia, blasts of pure erythroleukemia and acute megakaryoblastic leukemia exhibit intense nuclear GATA1 positivity, while blasts of acute myeloid leukemia of other categories are negative. GATA1 is also absent in the blasts of acute lymphoblastic leukemia/lymphoma and in the neoplastic cells of metastatic carcinoma and plasma cell neoplasms.
Conclusions:Intense GATA1 nuclear expression is a sensitive and specific marker for cells of erythroid and megakaryocytic lineages and is an excellent marker for neoplastic cells of pure erythroleukemia and acute megakaryoblastic leukemia. |
| doi_str_mv | 10.1093/ajcp/aqx018 |
| format | Article |
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Objectives: GATA binding factor 1 (GATA1) is a transcription factor essential for erythromegakaryocytic differentiation. Given its function in lineage specification, we sought to evaluate the immunohistochemical profile of GATA1 in normal marrow and acute leukemia and assess the use of GATA1 as a specific erythromegakaryocytic immunohistochemical marker.
Methods: Immunohistochemical studies for GATA1 expression were performed on bone marrow biopsy specimens to define its role in the evaluation of acute leukemia and other hematologic disorders.
Results: In normal marrows, intense nuclear reactivity is seen in immature erythroid precursors and megakaryocytes. Weak to moderate nuclear positivity is seen in eosinophils and mast cells. In marrows involved by acute leukemia, blasts of pure erythroleukemia and acute megakaryoblastic leukemia exhibit intense nuclear GATA1 positivity, while blasts of acute myeloid leukemia of other categories are negative. GATA1 is also absent in the blasts of acute lymphoblastic leukemia/lymphoma and in the neoplastic cells of metastatic carcinoma and plasma cell neoplasms.
Conclusions:Intense GATA1 nuclear expression is a sensitive and specific marker for cells of erythroid and megakaryocytic lineages and is an excellent marker for neoplastic cells of pure erythroleukemia and acute megakaryoblastic leukemia.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/ajcp/aqx018</identifier><identifier>PMID: 28340113</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Acute lymphoblastic leukemia ; Acute myeloid leukemia ; Biopsy ; Bone marrow ; Bone Marrow - metabolism ; Bone Marrow - pathology ; Cell Differentiation ; Cell Nucleus - metabolism ; Erythroid Cells - metabolism ; Erythroid Cells - pathology ; Erythroleukemia ; GATA-1 protein ; GATA1 Transcription Factor - genetics ; GATA1 Transcription Factor - metabolism ; Genetic Markers - genetics ; Hematological diseases ; Humans ; Leukemia ; Leukemia, Erythroblastic, Acute - diagnosis ; Leukemia, Erythroblastic, Acute - metabolism ; Leukemia, Erythroblastic, Acute - pathology ; Leukemia, Megakaryoblastic, Acute - diagnosis ; Leukemia, Megakaryoblastic, Acute - metabolism ; Leukemia, Megakaryoblastic, Acute - pathology ; Leukocytes (eosinophilic) ; Lymphatic leukemia ; Lymphoma ; Mast cells ; Megakaryocytes ; Megakaryocytes - metabolism ; Megakaryocytes - pathology ; Metastases ; Myeloid leukemia ; Neoplasia ; Original ; Sensitivity and Specificity</subject><ispartof>American journal of clinical pathology, 2017-04, Vol.147 (4), p.420-426</ispartof><rights>American Society for Clinical Pathology, 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2017</rights><rights>American Society for Clinical Pathology, 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><rights>Copyright Oxford University Press Apr 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-68caef9a6298c4f24c7a06e6d0b698ae2e36140d43c6b2d54a32c458b6c6fcdb3</citedby><cites>FETCH-LOGICAL-c440t-68caef9a6298c4f24c7a06e6d0b698ae2e36140d43c6b2d54a32c458b6c6fcdb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28340113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Winston Y.</creatorcontrib><creatorcontrib>Weinberg, Olga K.</creatorcontrib><creatorcontrib>Pinkus, Geraldine S.</creatorcontrib><title>GATA1 Is a Sensitive and Specific Nuclear Marker for Erythroid and Megakaryocytic Lineages</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>Abstract
Objectives: GATA binding factor 1 (GATA1) is a transcription factor essential for erythromegakaryocytic differentiation. Given its function in lineage specification, we sought to evaluate the immunohistochemical profile of GATA1 in normal marrow and acute leukemia and assess the use of GATA1 as a specific erythromegakaryocytic immunohistochemical marker.
Methods: Immunohistochemical studies for GATA1 expression were performed on bone marrow biopsy specimens to define its role in the evaluation of acute leukemia and other hematologic disorders.
Results: In normal marrows, intense nuclear reactivity is seen in immature erythroid precursors and megakaryocytes. Weak to moderate nuclear positivity is seen in eosinophils and mast cells. In marrows involved by acute leukemia, blasts of pure erythroleukemia and acute megakaryoblastic leukemia exhibit intense nuclear GATA1 positivity, while blasts of acute myeloid leukemia of other categories are negative. GATA1 is also absent in the blasts of acute lymphoblastic leukemia/lymphoma and in the neoplastic cells of metastatic carcinoma and plasma cell neoplasms.
Conclusions:Intense GATA1 nuclear expression is a sensitive and specific marker for cells of erythroid and megakaryocytic lineages and is an excellent marker for neoplastic cells of pure erythroleukemia and acute megakaryoblastic leukemia.</description><subject>Acute lymphoblastic leukemia</subject><subject>Acute myeloid leukemia</subject><subject>Biopsy</subject><subject>Bone marrow</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow - pathology</subject><subject>Cell Differentiation</subject><subject>Cell Nucleus - metabolism</subject><subject>Erythroid Cells - metabolism</subject><subject>Erythroid Cells - pathology</subject><subject>Erythroleukemia</subject><subject>GATA-1 protein</subject><subject>GATA1 Transcription Factor - genetics</subject><subject>GATA1 Transcription Factor - metabolism</subject><subject>Genetic Markers - genetics</subject><subject>Hematological diseases</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Leukemia, Erythroblastic, Acute - diagnosis</subject><subject>Leukemia, Erythroblastic, Acute - metabolism</subject><subject>Leukemia, Erythroblastic, Acute - pathology</subject><subject>Leukemia, Megakaryoblastic, Acute - diagnosis</subject><subject>Leukemia, Megakaryoblastic, Acute - metabolism</subject><subject>Leukemia, Megakaryoblastic, Acute - pathology</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lymphatic leukemia</subject><subject>Lymphoma</subject><subject>Mast cells</subject><subject>Megakaryocytes</subject><subject>Megakaryocytes - metabolism</subject><subject>Megakaryocytes - pathology</subject><subject>Metastases</subject><subject>Myeloid leukemia</subject><subject>Neoplasia</subject><subject>Original</subject><subject>Sensitivity and Specificity</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp90c1LHDEYBvAgFt1qT95LoCAFmZqvzWQuwiLWCmt7UC-9hHcy76xZZydjMiPuf9_RtVJ78JTD--PhCQ8hB5x946yQx7B03THcPzJutsiEF0pmeS7ENpkwxkRW8Fzuko8pLRnjwjC1Q3aFkYpxLifk9_nsesbpRaJAr7BNvvcPSKGt6FWHztfe0Z-DaxAivYR4h5HWIdKzuO5vY_DVs7zEBdxBXAe37kc_9y3CAtM--VBDk_DTy7tHbr6fXZ_-yOa_zi9OZ_PMKcX6TBsHWBegRWGcqoVyOTCNumKlLgygQKm5YpWSTpeimiqQwqmpKbXTtatKuUdONrndUK6wctj2ERrbRb8aS9kA3r69tP7WLsKDnRplZG7GgK8vATHcD5h6u_LJYdNAi2FIlhvDhc6NliP98h9dhiG24_es4HqqpWFCjepoo1wMKUWsX8twZp82s0-b2c1mo_78b_9X-3ekERxuQBi6d5P-ADUhoKg</recordid><startdate>20170401</startdate><enddate>20170401</enddate><creator>Lee, Winston Y.</creator><creator>Weinberg, Olga K.</creator><creator>Pinkus, Geraldine S.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170401</creationdate><title>GATA1 Is a Sensitive and Specific Nuclear Marker for Erythroid and Megakaryocytic Lineages</title><author>Lee, Winston Y. ; Weinberg, Olga K. ; Pinkus, Geraldine S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-68caef9a6298c4f24c7a06e6d0b698ae2e36140d43c6b2d54a32c458b6c6fcdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Acute lymphoblastic leukemia</topic><topic>Acute myeloid leukemia</topic><topic>Biopsy</topic><topic>Bone marrow</topic><topic>Bone Marrow - metabolism</topic><topic>Bone Marrow - pathology</topic><topic>Cell Differentiation</topic><topic>Cell Nucleus - metabolism</topic><topic>Erythroid Cells - metabolism</topic><topic>Erythroid Cells - pathology</topic><topic>Erythroleukemia</topic><topic>GATA-1 protein</topic><topic>GATA1 Transcription Factor - genetics</topic><topic>GATA1 Transcription Factor - metabolism</topic><topic>Genetic Markers - genetics</topic><topic>Hematological diseases</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Leukemia, Erythroblastic, Acute - diagnosis</topic><topic>Leukemia, Erythroblastic, Acute - metabolism</topic><topic>Leukemia, Erythroblastic, Acute - pathology</topic><topic>Leukemia, Megakaryoblastic, Acute - diagnosis</topic><topic>Leukemia, Megakaryoblastic, Acute - metabolism</topic><topic>Leukemia, Megakaryoblastic, Acute - pathology</topic><topic>Leukocytes (eosinophilic)</topic><topic>Lymphatic leukemia</topic><topic>Lymphoma</topic><topic>Mast cells</topic><topic>Megakaryocytes</topic><topic>Megakaryocytes - metabolism</topic><topic>Megakaryocytes - pathology</topic><topic>Metastases</topic><topic>Myeloid leukemia</topic><topic>Neoplasia</topic><topic>Original</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Winston Y.</creatorcontrib><creatorcontrib>Weinberg, Olga K.</creatorcontrib><creatorcontrib>Pinkus, Geraldine S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Winston Y.</au><au>Weinberg, Olga K.</au><au>Pinkus, Geraldine S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GATA1 Is a Sensitive and Specific Nuclear Marker for Erythroid and Megakaryocytic Lineages</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>2017-04-01</date><risdate>2017</risdate><volume>147</volume><issue>4</issue><spage>420</spage><epage>426</epage><pages>420-426</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><abstract>Abstract
Objectives: GATA binding factor 1 (GATA1) is a transcription factor essential for erythromegakaryocytic differentiation. Given its function in lineage specification, we sought to evaluate the immunohistochemical profile of GATA1 in normal marrow and acute leukemia and assess the use of GATA1 as a specific erythromegakaryocytic immunohistochemical marker.
Methods: Immunohistochemical studies for GATA1 expression were performed on bone marrow biopsy specimens to define its role in the evaluation of acute leukemia and other hematologic disorders.
Results: In normal marrows, intense nuclear reactivity is seen in immature erythroid precursors and megakaryocytes. Weak to moderate nuclear positivity is seen in eosinophils and mast cells. In marrows involved by acute leukemia, blasts of pure erythroleukemia and acute megakaryoblastic leukemia exhibit intense nuclear GATA1 positivity, while blasts of acute myeloid leukemia of other categories are negative. GATA1 is also absent in the blasts of acute lymphoblastic leukemia/lymphoma and in the neoplastic cells of metastatic carcinoma and plasma cell neoplasms.
Conclusions:Intense GATA1 nuclear expression is a sensitive and specific marker for cells of erythroid and megakaryocytic lineages and is an excellent marker for neoplastic cells of pure erythroleukemia and acute megakaryoblastic leukemia.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>28340113</pmid><doi>10.1093/ajcp/aqx018</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acute lymphoblastic leukemia Acute myeloid leukemia Biopsy Bone marrow Bone Marrow - metabolism Bone Marrow - pathology Cell Differentiation Cell Nucleus - metabolism Erythroid Cells - metabolism Erythroid Cells - pathology Erythroleukemia GATA-1 protein GATA1 Transcription Factor - genetics GATA1 Transcription Factor - metabolism Genetic Markers - genetics Hematological diseases Humans Leukemia Leukemia, Erythroblastic, Acute - diagnosis Leukemia, Erythroblastic, Acute - metabolism Leukemia, Erythroblastic, Acute - pathology Leukemia, Megakaryoblastic, Acute - diagnosis Leukemia, Megakaryoblastic, Acute - metabolism Leukemia, Megakaryoblastic, Acute - pathology Leukocytes (eosinophilic) Lymphatic leukemia Lymphoma Mast cells Megakaryocytes Megakaryocytes - metabolism Megakaryocytes - pathology Metastases Myeloid leukemia Neoplasia Original Sensitivity and Specificity |
| title | GATA1 Is a Sensitive and Specific Nuclear Marker for Erythroid and Megakaryocytic Lineages |
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