ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependence
Alcohol dependence is a worldwide problem with a great social and economic burden in many countries. A number of studies have suggested that BDNF (mature BDNF) and its precursor (proBDNF) play important roles in the alcohol dependence. However, what roles of the mBDNF/proBDNF pathways play during th...
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| creator | Zhou, Li Xiong, Jing Ruan, Chun-Sheng Ruan, Ye Liu, Dennis Bao, Jian-Jun Zhou, Xin-Fu |
| description | Alcohol dependence is a worldwide problem with a great social and economic burden in many countries. A number of studies have suggested that BDNF (mature BDNF) and its precursor (proBDNF) play important roles in the alcohol dependence. However, what roles of the mBDNF/proBDNF pathways play during the pathological process of alcohol dependence are not clearly understood. In our clinical study, peripheral blood was sampled from 30 male patients with alcohol dependence and 50 healthy males (as control). The protein levels of proBDNF, p75NTR, sortilin, mBDNF, TrkB and mRNA levels of
BDNF
,
p75NTR
,
sortilin
, and
TrkB
were detected in the peripheral blood in our study. We found that the protein levels of proBDNF and p75NTR were increased, but not the sortilin protein level; while the TrkB protein level was decreased in the alcohol dependence patients compared with healthy controls. Moreover, the mRNA levels of
p75NTR
and
sortilin
from the lymphocytes were slightly increased; while
BDNF
and
TrkB
were significantly decreased. The ELISA results of mBDNF and TrkB were declined in the alcohol dependence group. The levels of mBDNF and TrkB were negatively correlated with the average amount of daily ethanol consumption, and the levels of proBDNF, p75NTR and sortilin were positively correlated with the average amount of ethanol consumption per day. The ratio of proBDNF to mBDNF was altered in alcohol dependence patients. The balance between the proBDNF/p75NTR and mBDNF/TrkB signalling pathways appeared dysregulated in alcohol dependence. Our results suggested that both pathways may participate in the complex processes of alcohol dependence. |
| doi_str_mv | 10.1038/s41398-017-0015-4 |
| format | Article |
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BDNF
,
p75NTR
,
sortilin
, and
TrkB
were detected in the peripheral blood in our study. We found that the protein levels of proBDNF and p75NTR were increased, but not the sortilin protein level; while the TrkB protein level was decreased in the alcohol dependence patients compared with healthy controls. Moreover, the mRNA levels of
p75NTR
and
sortilin
from the lymphocytes were slightly increased; while
BDNF
and
TrkB
were significantly decreased. The ELISA results of mBDNF and TrkB were declined in the alcohol dependence group. The levels of mBDNF and TrkB were negatively correlated with the average amount of daily ethanol consumption, and the levels of proBDNF, p75NTR and sortilin were positively correlated with the average amount of ethanol consumption per day. The ratio of proBDNF to mBDNF was altered in alcohol dependence patients. The balance between the proBDNF/p75NTR and mBDNF/TrkB signalling pathways appeared dysregulated in alcohol dependence. Our results suggested that both pathways may participate in the complex processes of alcohol dependence.</description><identifier>ISSN: 2158-3188</identifier><identifier>EISSN: 2158-3188</identifier><identifier>DOI: 10.1038/s41398-017-0015-4</identifier><identifier>PMID: 29520063</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/378/340 ; 692/699/476/5 ; Adaptor Proteins, Vesicular Transport - blood ; Adult ; Alcohol use ; Alcoholism ; Alcoholism - blood ; Behavioral Sciences ; Biological Psychology ; Brain-Derived Neurotrophic Factor - blood ; Humans ; Male ; Medicine ; Medicine & Public Health ; Membrane Glycoproteins - blood ; Middle Aged ; Nerve Tissue Proteins - blood ; Neurosciences ; Pharmacotherapy ; Protein Precursors - blood ; Proteins ; Psychiatry ; Receptor, trkB - blood ; Receptors, Nerve Growth Factor - blood ; RNA, Messenger - blood ; Signal Transduction</subject><ispartof>Translational psychiatry, 2018-03, Vol.7 (11), p.2-10, Article 2</ispartof><rights>The Author(s) 2017</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-1f01ae1efb7b665a2a3bab46f254f5e20f5118da902340445a7027eaae226d573</citedby><cites>FETCH-LOGICAL-c470t-1f01ae1efb7b665a2a3bab46f254f5e20f5118da902340445a7027eaae226d573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843592/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843592/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,41125,42194,51581,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29520063$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Li</creatorcontrib><creatorcontrib>Xiong, Jing</creatorcontrib><creatorcontrib>Ruan, Chun-Sheng</creatorcontrib><creatorcontrib>Ruan, Ye</creatorcontrib><creatorcontrib>Liu, Dennis</creatorcontrib><creatorcontrib>Bao, Jian-Jun</creatorcontrib><creatorcontrib>Zhou, Xin-Fu</creatorcontrib><title>ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependence</title><title>Translational psychiatry</title><addtitle>Transl Psychiatry</addtitle><addtitle>Transl Psychiatry</addtitle><description>Alcohol dependence is a worldwide problem with a great social and economic burden in many countries. A number of studies have suggested that BDNF (mature BDNF) and its precursor (proBDNF) play important roles in the alcohol dependence. However, what roles of the mBDNF/proBDNF pathways play during the pathological process of alcohol dependence are not clearly understood. In our clinical study, peripheral blood was sampled from 30 male patients with alcohol dependence and 50 healthy males (as control). The protein levels of proBDNF, p75NTR, sortilin, mBDNF, TrkB and mRNA levels of
BDNF
,
p75NTR
,
sortilin
, and
TrkB
were detected in the peripheral blood in our study. We found that the protein levels of proBDNF and p75NTR were increased, but not the sortilin protein level; while the TrkB protein level was decreased in the alcohol dependence patients compared with healthy controls. Moreover, the mRNA levels of
p75NTR
and
sortilin
from the lymphocytes were slightly increased; while
BDNF
and
TrkB
were significantly decreased. The ELISA results of mBDNF and TrkB were declined in the alcohol dependence group. The levels of mBDNF and TrkB were negatively correlated with the average amount of daily ethanol consumption, and the levels of proBDNF, p75NTR and sortilin were positively correlated with the average amount of ethanol consumption per day. The ratio of proBDNF to mBDNF was altered in alcohol dependence patients. The balance between the proBDNF/p75NTR and mBDNF/TrkB signalling pathways appeared dysregulated in alcohol dependence. Our results suggested that both pathways may participate in the complex processes of alcohol dependence.</description><subject>631/378/340</subject><subject>692/699/476/5</subject><subject>Adaptor Proteins, Vesicular Transport - blood</subject><subject>Adult</subject><subject>Alcohol use</subject><subject>Alcoholism</subject><subject>Alcoholism - blood</subject><subject>Behavioral Sciences</subject><subject>Biological Psychology</subject><subject>Brain-Derived Neurotrophic Factor - blood</subject><subject>Humans</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Membrane Glycoproteins - blood</subject><subject>Middle Aged</subject><subject>Nerve Tissue Proteins - blood</subject><subject>Neurosciences</subject><subject>Pharmacotherapy</subject><subject>Protein Precursors - blood</subject><subject>Proteins</subject><subject>Psychiatry</subject><subject>Receptor, trkB - blood</subject><subject>Receptors, Nerve Growth Factor - blood</subject><subject>RNA, Messenger - blood</subject><subject>Signal Transduction</subject><issn>2158-3188</issn><issn>2158-3188</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kc1O3TAQha2qVUGUB-imstRNN-n1OHacbCq1_LRICBCia2uSTIhRbpzauSDeHkeXUqiEN7Y835w5o8PYRxBfQeTlKirIqzITYDIhQGfqDduVoMssh7J8--y9w_ZjvBHpaFWCgfdsR1ZaClHku6y9CP7H4dnxajL67OpyFX2Y3eBGPuHc3-E9d5FjM7tbnKnlyz8FN_UUcOD14H3LfbewjsY58js39xyHxvd-4C1NNLY0NvSBvetwiLT_eO-x38dHVwe_stPznycH30-zRhkxZ9AJQALqalMXhUaJeY21KjqpVadJik4DlC1WQuZKKKXRCGkIkaQsWm3yPfZtqztt6jW1TfKUfNopuDWGe-vR2ZeV0fX22t9aXapcVzIJfHkUCP7PhuJs1y42NAw4kt9EKwXICowullmf_0Nv_CaMab2FqpQuKlMkCrZUE3yMgbonMyDsEqPdxmhTjHaJ0arU8-n5Fk8df0NLgNwCMZXGawr_Rr-u-gDk-KhT</recordid><startdate>20180309</startdate><enddate>20180309</enddate><creator>Zhou, Li</creator><creator>Xiong, Jing</creator><creator>Ruan, Chun-Sheng</creator><creator>Ruan, Ye</creator><creator>Liu, Dennis</creator><creator>Bao, Jian-Jun</creator><creator>Zhou, Xin-Fu</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180309</creationdate><title>ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependence</title><author>Zhou, Li ; Xiong, Jing ; Ruan, Chun-Sheng ; Ruan, Ye ; Liu, Dennis ; Bao, Jian-Jun ; Zhou, Xin-Fu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-1f01ae1efb7b665a2a3bab46f254f5e20f5118da902340445a7027eaae226d573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>631/378/340</topic><topic>692/699/476/5</topic><topic>Adaptor Proteins, Vesicular Transport - blood</topic><topic>Adult</topic><topic>Alcohol use</topic><topic>Alcoholism</topic><topic>Alcoholism - blood</topic><topic>Behavioral Sciences</topic><topic>Biological Psychology</topic><topic>Brain-Derived Neurotrophic Factor - blood</topic><topic>Humans</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Membrane Glycoproteins - blood</topic><topic>Middle Aged</topic><topic>Nerve Tissue Proteins - blood</topic><topic>Neurosciences</topic><topic>Pharmacotherapy</topic><topic>Protein Precursors - blood</topic><topic>Proteins</topic><topic>Psychiatry</topic><topic>Receptor, trkB - blood</topic><topic>Receptors, Nerve Growth Factor - blood</topic><topic>RNA, Messenger - blood</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Li</creatorcontrib><creatorcontrib>Xiong, Jing</creatorcontrib><creatorcontrib>Ruan, Chun-Sheng</creatorcontrib><creatorcontrib>Ruan, Ye</creatorcontrib><creatorcontrib>Liu, Dennis</creatorcontrib><creatorcontrib>Bao, Jian-Jun</creatorcontrib><creatorcontrib>Zhou, Xin-Fu</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Translational psychiatry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Li</au><au>Xiong, Jing</au><au>Ruan, Chun-Sheng</au><au>Ruan, Ye</au><au>Liu, Dennis</au><au>Bao, Jian-Jun</au><au>Zhou, Xin-Fu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependence</atitle><jtitle>Translational psychiatry</jtitle><stitle>Transl Psychiatry</stitle><addtitle>Transl Psychiatry</addtitle><date>2018-03-09</date><risdate>2018</risdate><volume>7</volume><issue>11</issue><spage>2</spage><epage>10</epage><pages>2-10</pages><artnum>2</artnum><issn>2158-3188</issn><eissn>2158-3188</eissn><abstract>Alcohol dependence is a worldwide problem with a great social and economic burden in many countries. A number of studies have suggested that BDNF (mature BDNF) and its precursor (proBDNF) play important roles in the alcohol dependence. However, what roles of the mBDNF/proBDNF pathways play during the pathological process of alcohol dependence are not clearly understood. In our clinical study, peripheral blood was sampled from 30 male patients with alcohol dependence and 50 healthy males (as control). The protein levels of proBDNF, p75NTR, sortilin, mBDNF, TrkB and mRNA levels of
BDNF
,
p75NTR
,
sortilin
, and
TrkB
were detected in the peripheral blood in our study. We found that the protein levels of proBDNF and p75NTR were increased, but not the sortilin protein level; while the TrkB protein level was decreased in the alcohol dependence patients compared with healthy controls. Moreover, the mRNA levels of
p75NTR
and
sortilin
from the lymphocytes were slightly increased; while
BDNF
and
TrkB
were significantly decreased. The ELISA results of mBDNF and TrkB were declined in the alcohol dependence group. The levels of mBDNF and TrkB were negatively correlated with the average amount of daily ethanol consumption, and the levels of proBDNF, p75NTR and sortilin were positively correlated with the average amount of ethanol consumption per day. The ratio of proBDNF to mBDNF was altered in alcohol dependence patients. The balance between the proBDNF/p75NTR and mBDNF/TrkB signalling pathways appeared dysregulated in alcohol dependence. Our results suggested that both pathways may participate in the complex processes of alcohol dependence.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29520063</pmid><doi>10.1038/s41398-017-0015-4</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Springer Nature OA/Free Journals |
| subjects | 631/378/340 692/699/476/5 Adaptor Proteins, Vesicular Transport - blood Adult Alcohol use Alcoholism Alcoholism - blood Behavioral Sciences Biological Psychology Brain-Derived Neurotrophic Factor - blood Humans Male Medicine Medicine & Public Health Membrane Glycoproteins - blood Middle Aged Nerve Tissue Proteins - blood Neurosciences Pharmacotherapy Protein Precursors - blood Proteins Psychiatry Receptor, trkB - blood Receptors, Nerve Growth Factor - blood RNA, Messenger - blood Signal Transduction |
| title | ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependence |
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