Genome-wide identification and expression analysis of GRAS family transcription factors in tea plant (Camellia sinensis)

GRAS proteins are important transcription factors that play multifarious roles in regulating the growth and development as well as stress responses of plants. Tea plant is an economically important leaf -type beverage crop. Information concerning GRAS family transcription factors in tea plant is ins...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Scientific reports 2018-03, Vol.8 (1), p.3949-19, Article 3949
Hauptverfasser:	Wang, Yong-Xin, Liu, Zhi-Wei, Wu, Zhi-Jun, Li, Hui, Wang, Wen-Li, Cui, Xin, Zhuang, Jing
Format:	Artikel
Sprache:	eng
Schlagworte:	38 38/22 38/39 38/47 38/77 631/449/711 631/61/338/318 Amino Acid Sequence Camellia sinensis - genetics Camellia sinensis - metabolism Camellia sinensis - physiology Cold Temperature Conserved sequence Cultivars Droughts Economic importance Gene expression Gene Expression Profiling Gene Expression Regulation, Plant Genome, Plant Genomes Hot Temperature Humanities and Social Sciences multidisciplinary Phylogeny Proteins Science Science (multidisciplinary) Sequence Homology, Amino Acid Stress, Physiological Tea Transcription factors Transcription Factors - chemistry Transcription Factors - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	19
container_issue	1
container_start_page	3949
container_title	Scientific reports
container_volume	8
creator	Wang, Yong-Xin Liu, Zhi-Wei Wu, Zhi-Jun Li, Hui Wang, Wen-Li Cui, Xin Zhuang, Jing
description	GRAS proteins are important transcription factors that play multifarious roles in regulating the growth and development as well as stress responses of plants. Tea plant is an economically important leaf -type beverage crop. Information concerning GRAS family transcription factors in tea plant is insufficient. In this study, 52 CsGRAS genes encoding GRAS proteins were identified from tea plant genome database. Phylogenetic analysis of the identified GRAS proteins from tea plant, Arabidopsis, and rice divided these proteins into at least 13 subgroups. Conserved motif analysis revealed that the gene structure and motif compositions of the proteins were considerably conserved among the same subgroup. Functional divergence analysis indicated that the shifted evolutionary rate might act as a major evolutionary force driving subfamily-specific functional diversification. Transcriptome analysis showed that the transcriptional levels of CsGRAS genes under non-stress conditions varied among different tea plant cultivars. qRT-PCR analysis revealed tissue and development stage-specific expression patterns of CsGRAS genes in tea plant. The expression patterns of CsGRAS genes in response to abiotic stresses and gibberellin treatment suggested the possible multiple functions of these genes. This study provides insights into the potential functions of GRAS genes.
doi_str_mv	10.1038/s41598-018-22275-z
format	Article
fullrecord	<record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5834537</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2009878207</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-da933b1bf9c891a2c174b297f496b3a9aea04edc60ad0954cac04dcda345590e3</originalsourceid><addsrcrecordid>eNp9kU9rFTEUxYMottR-ARcScFMXo_nbmWyE8tCnUChYXYc7mUxNmUnG3Hm1r5_evE5bq4sGQhLuOb_k5hDymrP3nMnmAyquTVMx3lRCiFpXN8_IvmBKV0IK8fzRfo8cIl6yMrQwipuXZE8YzZhSzT65XvuYRl_9Dp2nZcY59MHBHFKkEDvqr6fsEZcjDFsMSFNP199OzmkPYxi2dM4Q0eUw3Zp6cHPKSEOkswc6DRBnerSC0Q9DAIoh-lgg716RFz0M6A_v1gPy4_On76sv1enZ-uvq5LRymvO56sBI2fK2N64xHITjtWqFqXtljlsJBjww5Tt3zKBjRisHjqnOdSCV1oZ5eUA-Ltxp045FWDrMMNgphxHy1iYI9t9KDD_tRbqyuikIWRfA0R0gp18bj7MdA7rSDUSfNmgFK3nUUihRpG__k16mTS7ftlMx09SNYDugWFQuJ8Ts-4fHcGZ32dolW1uytbfZ2ptievO4jQfLfZJFIBcBllK88Pnv3U9g_wD2eLJ6</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2009878207</pqid></control><display><type>article</type><title>Genome-wide identification and expression analysis of GRAS family transcription factors in tea plant (Camellia sinensis)</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Wang, Yong-Xin ; Liu, Zhi-Wei ; Wu, Zhi-Jun ; Li, Hui ; Wang, Wen-Li ; Cui, Xin ; Zhuang, Jing</creator><creatorcontrib>Wang, Yong-Xin ; Liu, Zhi-Wei ; Wu, Zhi-Jun ; Li, Hui ; Wang, Wen-Li ; Cui, Xin ; Zhuang, Jing</creatorcontrib><description>GRAS proteins are important transcription factors that play multifarious roles in regulating the growth and development as well as stress responses of plants. Tea plant is an economically important leaf -type beverage crop. Information concerning GRAS family transcription factors in tea plant is insufficient. In this study, 52 CsGRAS genes encoding GRAS proteins were identified from tea plant genome database. Phylogenetic analysis of the identified GRAS proteins from tea plant, Arabidopsis, and rice divided these proteins into at least 13 subgroups. Conserved motif analysis revealed that the gene structure and motif compositions of the proteins were considerably conserved among the same subgroup. Functional divergence analysis indicated that the shifted evolutionary rate might act as a major evolutionary force driving subfamily-specific functional diversification. Transcriptome analysis showed that the transcriptional levels of CsGRAS genes under non-stress conditions varied among different tea plant cultivars. qRT-PCR analysis revealed tissue and development stage-specific expression patterns of CsGRAS genes in tea plant. The expression patterns of CsGRAS genes in response to abiotic stresses and gibberellin treatment suggested the possible multiple functions of these genes. This study provides insights into the potential functions of GRAS genes.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-018-22275-z</identifier><identifier>PMID: 29500448</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38 ; 38/22 ; 38/39 ; 38/47 ; 38/77 ; 631/449/711 ; 631/61/338/318 ; Amino Acid Sequence ; Camellia sinensis - genetics ; Camellia sinensis - metabolism ; Camellia sinensis - physiology ; Cold Temperature ; Conserved sequence ; Cultivars ; Droughts ; Economic importance ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genome, Plant ; Genomes ; Hot Temperature ; Humanities and Social Sciences ; multidisciplinary ; Phylogeny ; Proteins ; Science ; Science (multidisciplinary) ; Sequence Homology, Amino Acid ; Stress, Physiological ; Tea ; Transcription factors ; Transcription Factors - chemistry ; Transcription Factors - metabolism</subject><ispartof>Scientific reports, 2018-03, Vol.8 (1), p.3949-19, Article 3949</ispartof><rights>The Author(s) 2018</rights><rights>2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-da933b1bf9c891a2c174b297f496b3a9aea04edc60ad0954cac04dcda345590e3</citedby><cites>FETCH-LOGICAL-c511t-da933b1bf9c891a2c174b297f496b3a9aea04edc60ad0954cac04dcda345590e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834537/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834537/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29500448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Yong-Xin</creatorcontrib><creatorcontrib>Liu, Zhi-Wei</creatorcontrib><creatorcontrib>Wu, Zhi-Jun</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Wang, Wen-Li</creatorcontrib><creatorcontrib>Cui, Xin</creatorcontrib><creatorcontrib>Zhuang, Jing</creatorcontrib><title>Genome-wide identification and expression analysis of GRAS family transcription factors in tea plant (Camellia sinensis)</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>GRAS proteins are important transcription factors that play multifarious roles in regulating the growth and development as well as stress responses of plants. Tea plant is an economically important leaf -type beverage crop. Information concerning GRAS family transcription factors in tea plant is insufficient. In this study, 52 CsGRAS genes encoding GRAS proteins were identified from tea plant genome database. Phylogenetic analysis of the identified GRAS proteins from tea plant, Arabidopsis, and rice divided these proteins into at least 13 subgroups. Conserved motif analysis revealed that the gene structure and motif compositions of the proteins were considerably conserved among the same subgroup. Functional divergence analysis indicated that the shifted evolutionary rate might act as a major evolutionary force driving subfamily-specific functional diversification. Transcriptome analysis showed that the transcriptional levels of CsGRAS genes under non-stress conditions varied among different tea plant cultivars. qRT-PCR analysis revealed tissue and development stage-specific expression patterns of CsGRAS genes in tea plant. The expression patterns of CsGRAS genes in response to abiotic stresses and gibberellin treatment suggested the possible multiple functions of these genes. This study provides insights into the potential functions of GRAS genes.</description><subject>38</subject><subject>38/22</subject><subject>38/39</subject><subject>38/47</subject><subject>38/77</subject><subject>631/449/711</subject><subject>631/61/338/318</subject><subject>Amino Acid Sequence</subject><subject>Camellia sinensis - genetics</subject><subject>Camellia sinensis - metabolism</subject><subject>Camellia sinensis - physiology</subject><subject>Cold Temperature</subject><subject>Conserved sequence</subject><subject>Cultivars</subject><subject>Droughts</subject><subject>Economic importance</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genome, Plant</subject><subject>Genomes</subject><subject>Hot Temperature</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sequence Homology, Amino Acid</subject><subject>Stress, Physiological</subject><subject>Tea</subject><subject>Transcription factors</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - metabolism</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU9rFTEUxYMottR-ARcScFMXo_nbmWyE8tCnUChYXYc7mUxNmUnG3Hm1r5_evE5bq4sGQhLuOb_k5hDymrP3nMnmAyquTVMx3lRCiFpXN8_IvmBKV0IK8fzRfo8cIl6yMrQwipuXZE8YzZhSzT65XvuYRl_9Dp2nZcY59MHBHFKkEDvqr6fsEZcjDFsMSFNP199OzmkPYxi2dM4Q0eUw3Zp6cHPKSEOkswc6DRBnerSC0Q9DAIoh-lgg716RFz0M6A_v1gPy4_On76sv1enZ-uvq5LRymvO56sBI2fK2N64xHITjtWqFqXtljlsJBjww5Tt3zKBjRisHjqnOdSCV1oZ5eUA-Ltxp045FWDrMMNgphxHy1iYI9t9KDD_tRbqyuikIWRfA0R0gp18bj7MdA7rSDUSfNmgFK3nUUihRpG__k16mTS7ftlMx09SNYDugWFQuJ8Ts-4fHcGZ32dolW1uytbfZ2ptievO4jQfLfZJFIBcBllK88Pnv3U9g_wD2eLJ6</recordid><startdate>20180302</startdate><enddate>20180302</enddate><creator>Wang, Yong-Xin</creator><creator>Liu, Zhi-Wei</creator><creator>Wu, Zhi-Jun</creator><creator>Li, Hui</creator><creator>Wang, Wen-Li</creator><creator>Cui, Xin</creator><creator>Zhuang, Jing</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180302</creationdate><title>Genome-wide identification and expression analysis of GRAS family transcription factors in tea plant (Camellia sinensis)</title><author>Wang, Yong-Xin ; Liu, Zhi-Wei ; Wu, Zhi-Jun ; Li, Hui ; Wang, Wen-Li ; Cui, Xin ; Zhuang, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-da933b1bf9c891a2c174b297f496b3a9aea04edc60ad0954cac04dcda345590e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>38</topic><topic>38/22</topic><topic>38/39</topic><topic>38/47</topic><topic>38/77</topic><topic>631/449/711</topic><topic>631/61/338/318</topic><topic>Amino Acid Sequence</topic><topic>Camellia sinensis - genetics</topic><topic>Camellia sinensis - metabolism</topic><topic>Camellia sinensis - physiology</topic><topic>Cold Temperature</topic><topic>Conserved sequence</topic><topic>Cultivars</topic><topic>Droughts</topic><topic>Economic importance</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genome, Plant</topic><topic>Genomes</topic><topic>Hot Temperature</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Phylogeny</topic><topic>Proteins</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sequence Homology, Amino Acid</topic><topic>Stress, Physiological</topic><topic>Tea</topic><topic>Transcription factors</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Yong-Xin</creatorcontrib><creatorcontrib>Liu, Zhi-Wei</creatorcontrib><creatorcontrib>Wu, Zhi-Jun</creatorcontrib><creatorcontrib>Li, Hui</creatorcontrib><creatorcontrib>Wang, Wen-Li</creatorcontrib><creatorcontrib>Cui, Xin</creatorcontrib><creatorcontrib>Zhuang, Jing</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Yong-Xin</au><au>Liu, Zhi-Wei</au><au>Wu, Zhi-Jun</au><au>Li, Hui</au><au>Wang, Wen-Li</au><au>Cui, Xin</au><au>Zhuang, Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide identification and expression analysis of GRAS family transcription factors in tea plant (Camellia sinensis)</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-03-02</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>3949</spage><epage>19</epage><pages>3949-19</pages><artnum>3949</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>GRAS proteins are important transcription factors that play multifarious roles in regulating the growth and development as well as stress responses of plants. Tea plant is an economically important leaf -type beverage crop. Information concerning GRAS family transcription factors in tea plant is insufficient. In this study, 52 CsGRAS genes encoding GRAS proteins were identified from tea plant genome database. Phylogenetic analysis of the identified GRAS proteins from tea plant, Arabidopsis, and rice divided these proteins into at least 13 subgroups. Conserved motif analysis revealed that the gene structure and motif compositions of the proteins were considerably conserved among the same subgroup. Functional divergence analysis indicated that the shifted evolutionary rate might act as a major evolutionary force driving subfamily-specific functional diversification. Transcriptome analysis showed that the transcriptional levels of CsGRAS genes under non-stress conditions varied among different tea plant cultivars. qRT-PCR analysis revealed tissue and development stage-specific expression patterns of CsGRAS genes in tea plant. The expression patterns of CsGRAS genes in response to abiotic stresses and gibberellin treatment suggested the possible multiple functions of these genes. This study provides insights into the potential functions of GRAS genes.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29500448</pmid><doi>10.1038/s41598-018-22275-z</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record>
fulltext	fulltext
identifier	ISSN: 2045-2322
ispartof	Scientific reports, 2018-03, Vol.8 (1), p.3949-19, Article 3949
issn	2045-2322 2045-2322
language	eng
recordid	cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5834537
source	MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals
subjects	38 38/22 38/39 38/47 38/77 631/449/711 631/61/338/318 Amino Acid Sequence Camellia sinensis - genetics Camellia sinensis - metabolism Camellia sinensis - physiology Cold Temperature Conserved sequence Cultivars Droughts Economic importance Gene expression Gene Expression Profiling Gene Expression Regulation, Plant Genome, Plant Genomes Hot Temperature Humanities and Social Sciences multidisciplinary Phylogeny Proteins Science Science (multidisciplinary) Sequence Homology, Amino Acid Stress, Physiological Tea Transcription factors Transcription Factors - chemistry Transcription Factors - metabolism
title	Genome-wide identification and expression analysis of GRAS family transcription factors in tea plant (Camellia sinensis)
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T00%3A30%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome-wide%20identification%20and%20expression%20analysis%20of%20GRAS%20family%20transcription%20factors%20in%20tea%20plant%20(Camellia%20sinensis)&rft.jtitle=Scientific%20reports&rft.au=Wang,%20Yong-Xin&rft.date=2018-03-02&rft.volume=8&rft.issue=1&rft.spage=3949&rft.epage=19&rft.pages=3949-19&rft.artnum=3949&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-018-22275-z&rft_dat=%3Cproquest_pubme%3E2009878207%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2009878207&rft_id=info:pmid/29500448&rfr_iscdi=true