The Abnormal Expression of MicroRNA-542-3p in Hepatocellular Carcinoma and Its Clinical Significance
Aim. To evaluate the expression of miRNA-542-3p in hepatocellular carcinoma, establish its function, and evaluate whether it could serve as a biomarker for diagnosis and prognosis of HCC patients. Methods. qRT-PCR analysis was performed to determine the expression level of miRNA-542-3p in normal liv...
Gespeichert in:
| Veröffentlicht in: | Disease markers 2018-01, Vol.2018 (2018), p.1-9 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9 |
|---|---|
| container_issue | 2018 |
| container_start_page | 1 |
| container_title | Disease markers |
| container_volume | 2018 |
| creator | Hong, Zhenfei Lan, Tian Ma, Weijie Zhang, Qi Chen, Xi Yuan, Yufeng |
| description | Aim. To evaluate the expression of miRNA-542-3p in hepatocellular carcinoma, establish its function, and evaluate whether it could serve as a biomarker for diagnosis and prognosis of HCC patients. Methods. qRT-PCR analysis was performed to determine the expression level of miRNA-542-3p in normal liver cells and HCC cell lines. Additionally, samples from TCGA consortium and from our patients were analyzed using biostatistical methods to ascertain whether miR-542-3p could be a good biomarker for HCC diagnosis and prognosis. The effects of miRNA-542-3p on HCC were investigated in HCCLM9 cells. Results. The expression of miRNA-542-3p in HCC cells was significantly downregulated compared with normal liver cells. A lower level of expression of miRNA-542-3p was found in HCC tissue samples than in adjacent normal liver tissue samples from TCGA cases and our patients. Further evaluation revealed that the downregulation was clearly related to aggressive clinicopathological characteristics and affected the prognosis, as low-expressing patients tended to have shorter overall survival. Moreover, cell assays revealed that miR-542-3p overexpression inhibited HCC cell growth and induced apoptosis. Conclusion. We demonstrated for the first time that miRNA-542-3p appears to function as a novel tumor suppressor in HCC and may serve as a promising prognostic biomarker in HCC patients. |
| doi_str_mv | 10.1155/2018/3973250 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5828045</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A587655825</galeid><sourcerecordid>A587655825</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-f8cce8c335a1e8920997fe0da82860d57df88532aae3832ce70188cc8cdbaecd3</originalsourceid><addsrcrecordid>eNqNkc-P1CAYhonRuOPqzbNp4sVE635AaeFiMpms7iarJrqeCUO_zrBpoULrj_9emhl31ZMnSHh4Xl4-Qp5SeE2pEGcMqDzjquFMwD2yorIRpaw53CcrYI0sgVVwQh6ldANAmarUQ3LCVA21kmJF2us9FuutD3EwfXH-Y4yYkgu-CF3x3tkYPn1Yl6JiJR8L54sLHM0ULPb93JtYbEy0zofBFMa3xeWUik3vvLNZ9dntvOvy1lt8TB50pk_45Lieki9vz683F-XVx3eXm_VVaSulprKT1qK0nAtDUSoGSjUdQmskkzW0omk7KQVnxiCXnFlscvV8R9p2a9C2_JS8OXjHeTtga9FP0fR6jG4w8acOxum_T7zb6134pkVOgEpkwYujIIavM6ZJDy4tbY3HMCfNgIFUtQKe0ef_oDdhjj7XyxQF2YDi9I7amR61813IuXaR6rWQTS1y8hL76kDl_04pYnf7ZAp6GfKilPo45Iw_-7PmLfx7qhl4eQD2zrfmu_tPHWYGO3NH0_zZivJfRw23fg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2010870931</pqid></control><display><type>article</type><title>The Abnormal Expression of MicroRNA-542-3p in Hepatocellular Carcinoma and Its Clinical Significance</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Hong, Zhenfei ; Lan, Tian ; Ma, Weijie ; Zhang, Qi ; Chen, Xi ; Yuan, Yufeng</creator><contributor>Peluso, Marco E. M. ; Marco E M Peluso</contributor><creatorcontrib>Hong, Zhenfei ; Lan, Tian ; Ma, Weijie ; Zhang, Qi ; Chen, Xi ; Yuan, Yufeng ; Peluso, Marco E. M. ; Marco E M Peluso</creatorcontrib><description>Aim. To evaluate the expression of miRNA-542-3p in hepatocellular carcinoma, establish its function, and evaluate whether it could serve as a biomarker for diagnosis and prognosis of HCC patients. Methods. qRT-PCR analysis was performed to determine the expression level of miRNA-542-3p in normal liver cells and HCC cell lines. Additionally, samples from TCGA consortium and from our patients were analyzed using biostatistical methods to ascertain whether miR-542-3p could be a good biomarker for HCC diagnosis and prognosis. The effects of miRNA-542-3p on HCC were investigated in HCCLM9 cells. Results. The expression of miRNA-542-3p in HCC cells was significantly downregulated compared with normal liver cells. A lower level of expression of miRNA-542-3p was found in HCC tissue samples than in adjacent normal liver tissue samples from TCGA cases and our patients. Further evaluation revealed that the downregulation was clearly related to aggressive clinicopathological characteristics and affected the prognosis, as low-expressing patients tended to have shorter overall survival. Moreover, cell assays revealed that miR-542-3p overexpression inhibited HCC cell growth and induced apoptosis. Conclusion. We demonstrated for the first time that miRNA-542-3p appears to function as a novel tumor suppressor in HCC and may serve as a promising prognostic biomarker in HCC patients.</description><identifier>ISSN: 0278-0240</identifier><identifier>EISSN: 1875-8630</identifier><identifier>DOI: 10.1155/2018/3973250</identifier><identifier>PMID: 29606985</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Apoptosis ; Biomarkers ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - pathology ; Cell Line, Tumor ; Cell Proliferation ; Cell survival ; Clinical significance ; Comparative analysis ; Consortia ; Development and progression ; Diagnosis ; Down-Regulation ; Ethylenediaminetetraacetic acid ; Female ; Gene Expression Regulation, Neoplastic ; Hep G2 Cells ; Hepatocellular carcinoma ; Hepatocytes ; Hepatoma ; Humans ; Liver ; Liver cancer ; Liver Neoplasms - genetics ; Liver Neoplasms - pathology ; Male ; Medical prognosis ; MicroRNA ; MicroRNAs - genetics ; miRNA ; Neoplasm Staging ; Patients ; Prognosis ; Ribonucleic acid ; RNA ; Survival Analysis ; Tumor suppressor genes</subject><ispartof>Disease markers, 2018-01, Vol.2018 (2018), p.1-9</ispartof><rights>Copyright © 2018 Xi Chen et al.</rights><rights>COPYRIGHT 2018 John Wiley & Sons, Inc.</rights><rights>Copyright © 2018 Xi Chen et al.; This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2018 Xi Chen et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-f8cce8c335a1e8920997fe0da82860d57df88532aae3832ce70188cc8cdbaecd3</citedby><cites>FETCH-LOGICAL-c499t-f8cce8c335a1e8920997fe0da82860d57df88532aae3832ce70188cc8cdbaecd3</cites><orcidid>0000-0003-3924-3803</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828045/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828045/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29606985$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Peluso, Marco E. M.</contributor><contributor>Marco E M Peluso</contributor><creatorcontrib>Hong, Zhenfei</creatorcontrib><creatorcontrib>Lan, Tian</creatorcontrib><creatorcontrib>Ma, Weijie</creatorcontrib><creatorcontrib>Zhang, Qi</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Yuan, Yufeng</creatorcontrib><title>The Abnormal Expression of MicroRNA-542-3p in Hepatocellular Carcinoma and Its Clinical Significance</title><title>Disease markers</title><addtitle>Dis Markers</addtitle><description>Aim. To evaluate the expression of miRNA-542-3p in hepatocellular carcinoma, establish its function, and evaluate whether it could serve as a biomarker for diagnosis and prognosis of HCC patients. Methods. qRT-PCR analysis was performed to determine the expression level of miRNA-542-3p in normal liver cells and HCC cell lines. Additionally, samples from TCGA consortium and from our patients were analyzed using biostatistical methods to ascertain whether miR-542-3p could be a good biomarker for HCC diagnosis and prognosis. The effects of miRNA-542-3p on HCC were investigated in HCCLM9 cells. Results. The expression of miRNA-542-3p in HCC cells was significantly downregulated compared with normal liver cells. A lower level of expression of miRNA-542-3p was found in HCC tissue samples than in adjacent normal liver tissue samples from TCGA cases and our patients. Further evaluation revealed that the downregulation was clearly related to aggressive clinicopathological characteristics and affected the prognosis, as low-expressing patients tended to have shorter overall survival. Moreover, cell assays revealed that miR-542-3p overexpression inhibited HCC cell growth and induced apoptosis. Conclusion. We demonstrated for the first time that miRNA-542-3p appears to function as a novel tumor suppressor in HCC and may serve as a promising prognostic biomarker in HCC patients.</description><subject>Apoptosis</subject><subject>Biomarkers</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Cell survival</subject><subject>Clinical significance</subject><subject>Comparative analysis</subject><subject>Consortia</subject><subject>Development and progression</subject><subject>Diagnosis</subject><subject>Down-Regulation</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hep G2 Cells</subject><subject>Hepatocellular carcinoma</subject><subject>Hepatocytes</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Liver</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - pathology</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>MicroRNA</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Neoplasm Staging</subject><subject>Patients</subject><subject>Prognosis</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Survival Analysis</subject><subject>Tumor suppressor genes</subject><issn>0278-0240</issn><issn>1875-8630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc-P1CAYhonRuOPqzbNp4sVE635AaeFiMpms7iarJrqeCUO_zrBpoULrj_9emhl31ZMnSHh4Xl4-Qp5SeE2pEGcMqDzjquFMwD2yorIRpaw53CcrYI0sgVVwQh6ldANAmarUQ3LCVA21kmJF2us9FuutD3EwfXH-Y4yYkgu-CF3x3tkYPn1Yl6JiJR8L54sLHM0ULPb93JtYbEy0zofBFMa3xeWUik3vvLNZ9dntvOvy1lt8TB50pk_45Lieki9vz683F-XVx3eXm_VVaSulprKT1qK0nAtDUSoGSjUdQmskkzW0omk7KQVnxiCXnFlscvV8R9p2a9C2_JS8OXjHeTtga9FP0fR6jG4w8acOxum_T7zb6134pkVOgEpkwYujIIavM6ZJDy4tbY3HMCfNgIFUtQKe0ef_oDdhjj7XyxQF2YDi9I7amR61813IuXaR6rWQTS1y8hL76kDl_04pYnf7ZAp6GfKilPo45Iw_-7PmLfx7qhl4eQD2zrfmu_tPHWYGO3NH0_zZivJfRw23fg</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Hong, Zhenfei</creator><creator>Lan, Tian</creator><creator>Ma, Weijie</creator><creator>Zhang, Qi</creator><creator>Chen, Xi</creator><creator>Yuan, Yufeng</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3924-3803</orcidid></search><sort><creationdate>20180101</creationdate><title>The Abnormal Expression of MicroRNA-542-3p in Hepatocellular Carcinoma and Its Clinical Significance</title><author>Hong, Zhenfei ; Lan, Tian ; Ma, Weijie ; Zhang, Qi ; Chen, Xi ; Yuan, Yufeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-f8cce8c335a1e8920997fe0da82860d57df88532aae3832ce70188cc8cdbaecd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Biomarkers</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Cell survival</topic><topic>Clinical significance</topic><topic>Comparative analysis</topic><topic>Consortia</topic><topic>Development and progression</topic><topic>Diagnosis</topic><topic>Down-Regulation</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hep G2 Cells</topic><topic>Hepatocellular carcinoma</topic><topic>Hepatocytes</topic><topic>Hepatoma</topic><topic>Humans</topic><topic>Liver</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - pathology</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>MicroRNA</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Neoplasm Staging</topic><topic>Patients</topic><topic>Prognosis</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Survival Analysis</topic><topic>Tumor suppressor genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hong, Zhenfei</creatorcontrib><creatorcontrib>Lan, Tian</creatorcontrib><creatorcontrib>Ma, Weijie</creatorcontrib><creatorcontrib>Zhang, Qi</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Yuan, Yufeng</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Disease markers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hong, Zhenfei</au><au>Lan, Tian</au><au>Ma, Weijie</au><au>Zhang, Qi</au><au>Chen, Xi</au><au>Yuan, Yufeng</au><au>Peluso, Marco E. M.</au><au>Marco E M Peluso</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Abnormal Expression of MicroRNA-542-3p in Hepatocellular Carcinoma and Its Clinical Significance</atitle><jtitle>Disease markers</jtitle><addtitle>Dis Markers</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>2018</volume><issue>2018</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0278-0240</issn><eissn>1875-8630</eissn><abstract>Aim. To evaluate the expression of miRNA-542-3p in hepatocellular carcinoma, establish its function, and evaluate whether it could serve as a biomarker for diagnosis and prognosis of HCC patients. Methods. qRT-PCR analysis was performed to determine the expression level of miRNA-542-3p in normal liver cells and HCC cell lines. Additionally, samples from TCGA consortium and from our patients were analyzed using biostatistical methods to ascertain whether miR-542-3p could be a good biomarker for HCC diagnosis and prognosis. The effects of miRNA-542-3p on HCC were investigated in HCCLM9 cells. Results. The expression of miRNA-542-3p in HCC cells was significantly downregulated compared with normal liver cells. A lower level of expression of miRNA-542-3p was found in HCC tissue samples than in adjacent normal liver tissue samples from TCGA cases and our patients. Further evaluation revealed that the downregulation was clearly related to aggressive clinicopathological characteristics and affected the prognosis, as low-expressing patients tended to have shorter overall survival. Moreover, cell assays revealed that miR-542-3p overexpression inhibited HCC cell growth and induced apoptosis. Conclusion. We demonstrated for the first time that miRNA-542-3p appears to function as a novel tumor suppressor in HCC and may serve as a promising prognostic biomarker in HCC patients.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>29606985</pmid><doi>10.1155/2018/3973250</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3924-3803</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0278-0240 |
| ispartof | Disease markers, 2018-01, Vol.2018 (2018), p.1-9 |
| issn | 0278-0240 1875-8630 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5828045 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Apoptosis Biomarkers Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - pathology Cell Line, Tumor Cell Proliferation Cell survival Clinical significance Comparative analysis Consortia Development and progression Diagnosis Down-Regulation Ethylenediaminetetraacetic acid Female Gene Expression Regulation, Neoplastic Hep G2 Cells Hepatocellular carcinoma Hepatocytes Hepatoma Humans Liver Liver cancer Liver Neoplasms - genetics Liver Neoplasms - pathology Male Medical prognosis MicroRNA MicroRNAs - genetics miRNA Neoplasm Staging Patients Prognosis Ribonucleic acid RNA Survival Analysis Tumor suppressor genes |
| title | The Abnormal Expression of MicroRNA-542-3p in Hepatocellular Carcinoma and Its Clinical Significance |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T17%3A58%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Abnormal%20Expression%20of%20MicroRNA-542-3p%20in%20Hepatocellular%20Carcinoma%20and%20Its%20Clinical%20Significance&rft.jtitle=Disease%20markers&rft.au=Hong,%20Zhenfei&rft.date=2018-01-01&rft.volume=2018&rft.issue=2018&rft.spage=1&rft.epage=9&rft.pages=1-9&rft.issn=0278-0240&rft.eissn=1875-8630&rft_id=info:doi/10.1155/2018/3973250&rft_dat=%3Cgale_pubme%3EA587655825%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2010870931&rft_id=info:pmid/29606985&rft_galeid=A587655825&rfr_iscdi=true |