Structural analysis of group II chitinase (ChtII) catalysis completes the puzzle of chitin hydrolysis in insects

Chitin is a linear homopolymer of N-acetyl-β-d-glucosamines and a major structural component of insect cuticles. Chitin hydrolysis involves glycoside hydrolase family 18 (GH18) chitinases. In insects, chitin hydrolysis is essential for periodic shedding of the old cuticle ecdysis and proceeds via a...

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Veröffentlicht in:	The Journal of biological chemistry 2018-02, Vol.293 (8), p.2652-2660
Hauptverfasser:	Chen, Wei, Qu, Mingbo, Zhou, Yong, Yang, Qing
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Animals Binding Sites Biocatalysis Catalytic Domain chitin Chitin - chemistry Chitin - metabolism chitin degradation chitinase Chitinases - chemistry Chitinases - genetics Chitinases - metabolism crystal structure Crystallography, X-Ray enzyme catalysis Enzyme Stability enzyme structure Enzymology Hydrolysis insect Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism Isoenzymes - chemistry Isoenzymes - genetics Isoenzymes - metabolism Lepidoptera Ligands Models, Molecular Mutation Oligosaccharides - chemistry Oligosaccharides - metabolism Ostrinia furnacalis Peptide Fragments - chemistry Peptide Fragments - genetics Peptide Fragments - metabolism Protein Conformation Protein Interaction Domains and Motifs Recombinant Proteins - chemistry Recombinant Proteins - metabolism Serratia marcescens Substrate Specificity
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