Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study
Introduction Non-medical use of prescription opioids such as the mu opioid receptor (MOP-r) agonist oxycodone is a growing problem in the USA and elsewhere. There is limited information about oxycodone’s impact on diverse gene systems in the brain. Objectives The current study was designed to examin...
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| Veröffentlicht in: | Psychopharmacology 2017-08, Vol.234 (15), p.2259-2275 |
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| creator | Zhang, Yong Liang, Yupu Levran, Orna Randesi, Matthew Yuferov, Vadim Zhao, Connie Kreek, Mary Jeanne |
| description | Introduction
Non-medical use of prescription opioids such as the mu opioid receptor (MOP-r) agonist oxycodone is a growing problem in the USA and elsewhere. There is limited information about oxycodone’s impact on diverse gene systems in the brain.
Objectives
The current study was designed to examine how chronic oxycodone self-administration (SA) affects gene expression in the terminal areas of the nigrostriatal and mesolimbic dopaminergic pathways in mice.
Method
Adult male C57BL/6J mice underwent a 14-day oxycodone self-administration procedure (4 h/day, 0.25 mg/kg/infusion, FR1) and were euthanized 1 h after the last session. The dorsal and ventral striata were dissected, and total RNAs were extracted. Gene expressions were examined using RNA sequencing.
Result
We found that oxycodone self-administration exposure led to alterations of expression in numerous genes related to inflammation/immune functions in the dorsal striatum (54 upregulated genes and 1 downregulated gene) and ventral striatum (126 upregulated genes and 15 downregulated genes), with 38 upregulated genes identified in both brain regions.
Conclusion
This study reveals novel neurobiological mechanisms underlying some of the effects of a commonly abused prescription opioid. We propose that inflammation/immune gene systems may undergo a major change during chronic self-administration of oxycodone. |
| doi_str_mv | 10.1007/s00213-017-4657-y |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5826641</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A498972373</galeid><sourcerecordid>A498972373</sourcerecordid><originalsourceid>FETCH-LOGICAL-c537t-abef8678bceffcf205d397f4aac36ebb9d2238fcc060de9fdd41b857399446963</originalsourceid><addsrcrecordid>eNp1UstuEzEUHSEQDYUPYIMssWEzjV_zYoEUIp6KQEKwtjz2deLKYwd7pjR_x6fhaUppEdgLP-45xz72KYqnBJ8RjJtlwpgSVmLSlLyumvJwr1gQzmhJcUPvFwuMGSsZqdqT4lFK5zg33vKHxQlt64rhFi-Knys3QpSjDT6hYBBc7iOklJfzynrj5DBclZd2GCYPZQQnR9BoCx5SRqBxB0iHmKRD0mt0AX6MeZ7GaOU4DbOO1JMb0bpqXm-W9Uc0WAXIBOfCD-u3SO1i8FahcHlQQQcPKIEzpdSD9TbLXB3_Ekn05dMql75P4NXMS-OkD4-LB0a6BE-ux9Pi29s3X9fvy83ndx_Wq02pKtaMpezBtHXT9gqMUYbiSrOuMVxKxWro-05TylqjFK6xhs5ozUnfVg3rOs7rrmanxauj7n7qB9Dq6FLsox1kPIggrbhb8XYntuFCVC2ta06ywItrgRiyhTSKwSYFzkkPYUqCdITTtms6nKHP_4Kehyn6bC-jaL4h46z9g9pKByJ_VcjnqllUrHiXlShrWEad_QOVu4b8Dfmxjc37dwjkSFAxpBTB3HgkWMyxE8fYiRw7McdOHDLn2e3HuWH8zlkG0CMg5ZLfQrzl6L-qvwAVeueV</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1920603438</pqid></control><display><type>article</type><title>Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study</title><source>SpringerLink Journals - AutoHoldings</source><creator>Zhang, Yong ; Liang, Yupu ; Levran, Orna ; Randesi, Matthew ; Yuferov, Vadim ; Zhao, Connie ; Kreek, Mary Jeanne</creator><creatorcontrib>Zhang, Yong ; Liang, Yupu ; Levran, Orna ; Randesi, Matthew ; Yuferov, Vadim ; Zhao, Connie ; Kreek, Mary Jeanne</creatorcontrib><description>Introduction
Non-medical use of prescription opioids such as the mu opioid receptor (MOP-r) agonist oxycodone is a growing problem in the USA and elsewhere. There is limited information about oxycodone’s impact on diverse gene systems in the brain.
Objectives
The current study was designed to examine how chronic oxycodone self-administration (SA) affects gene expression in the terminal areas of the nigrostriatal and mesolimbic dopaminergic pathways in mice.
Method
Adult male C57BL/6J mice underwent a 14-day oxycodone self-administration procedure (4 h/day, 0.25 mg/kg/infusion, FR1) and were euthanized 1 h after the last session. The dorsal and ventral striata were dissected, and total RNAs were extracted. Gene expressions were examined using RNA sequencing.
Result
We found that oxycodone self-administration exposure led to alterations of expression in numerous genes related to inflammation/immune functions in the dorsal striatum (54 upregulated genes and 1 downregulated gene) and ventral striatum (126 upregulated genes and 15 downregulated genes), with 38 upregulated genes identified in both brain regions.
Conclusion
This study reveals novel neurobiological mechanisms underlying some of the effects of a commonly abused prescription opioid. We propose that inflammation/immune gene systems may undergo a major change during chronic self-administration of oxycodone.</description><identifier>ISSN: 0033-3158</identifier><identifier>EISSN: 1432-2072</identifier><identifier>DOI: 10.1007/s00213-017-4657-y</identifier><identifier>PMID: 28653080</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Brain ; Caudate-putamen ; Complications and side effects ; Dopamine ; Dopamine receptors ; Drug abuse ; Drug interactions ; Gene expression ; Genes ; Genetic aspects ; Health aspects ; Immune response ; Inflammation ; Mesolimbic system ; Methods ; Mice ; Narcotics ; Neostriatum ; Neurosciences ; Opioid receptors (type mu) ; Original Investigation ; Oxycodone ; Pharmacology/Toxicology ; Psychiatry ; Revisions ; Ribonucleic acid ; RNA ; RNA sequencing ; Rodents ; Self-administration ; Striatum</subject><ispartof>Psychopharmacology, 2017-08, Vol.234 (15), p.2259-2275</ispartof><rights>Springer-Verlag Berlin Heidelberg 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Psychopharmacology is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-abef8678bceffcf205d397f4aac36ebb9d2238fcc060de9fdd41b857399446963</citedby><cites>FETCH-LOGICAL-c537t-abef8678bceffcf205d397f4aac36ebb9d2238fcc060de9fdd41b857399446963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00213-017-4657-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00213-017-4657-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28653080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Liang, Yupu</creatorcontrib><creatorcontrib>Levran, Orna</creatorcontrib><creatorcontrib>Randesi, Matthew</creatorcontrib><creatorcontrib>Yuferov, Vadim</creatorcontrib><creatorcontrib>Zhao, Connie</creatorcontrib><creatorcontrib>Kreek, Mary Jeanne</creatorcontrib><title>Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study</title><title>Psychopharmacology</title><addtitle>Psychopharmacology</addtitle><addtitle>Psychopharmacology (Berl)</addtitle><description>Introduction
Non-medical use of prescription opioids such as the mu opioid receptor (MOP-r) agonist oxycodone is a growing problem in the USA and elsewhere. There is limited information about oxycodone’s impact on diverse gene systems in the brain.
Objectives
The current study was designed to examine how chronic oxycodone self-administration (SA) affects gene expression in the terminal areas of the nigrostriatal and mesolimbic dopaminergic pathways in mice.
Method
Adult male C57BL/6J mice underwent a 14-day oxycodone self-administration procedure (4 h/day, 0.25 mg/kg/infusion, FR1) and were euthanized 1 h after the last session. The dorsal and ventral striata were dissected, and total RNAs were extracted. Gene expressions were examined using RNA sequencing.
Result
We found that oxycodone self-administration exposure led to alterations of expression in numerous genes related to inflammation/immune functions in the dorsal striatum (54 upregulated genes and 1 downregulated gene) and ventral striatum (126 upregulated genes and 15 downregulated genes), with 38 upregulated genes identified in both brain regions.
Conclusion
This study reveals novel neurobiological mechanisms underlying some of the effects of a commonly abused prescription opioid. We propose that inflammation/immune gene systems may undergo a major change during chronic self-administration of oxycodone.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain</subject><subject>Caudate-putamen</subject><subject>Complications and side effects</subject><subject>Dopamine</subject><subject>Dopamine receptors</subject><subject>Drug abuse</subject><subject>Drug interactions</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Immune response</subject><subject>Inflammation</subject><subject>Mesolimbic system</subject><subject>Methods</subject><subject>Mice</subject><subject>Narcotics</subject><subject>Neostriatum</subject><subject>Neurosciences</subject><subject>Opioid receptors (type mu)</subject><subject>Original Investigation</subject><subject>Oxycodone</subject><subject>Pharmacology/Toxicology</subject><subject>Psychiatry</subject><subject>Revisions</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA sequencing</subject><subject>Rodents</subject><subject>Self-administration</subject><subject>Striatum</subject><issn>0033-3158</issn><issn>1432-2072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1UstuEzEUHSEQDYUPYIMssWEzjV_zYoEUIp6KQEKwtjz2deLKYwd7pjR_x6fhaUppEdgLP-45xz72KYqnBJ8RjJtlwpgSVmLSlLyumvJwr1gQzmhJcUPvFwuMGSsZqdqT4lFK5zg33vKHxQlt64rhFi-Knys3QpSjDT6hYBBc7iOklJfzynrj5DBclZd2GCYPZQQnR9BoCx5SRqBxB0iHmKRD0mt0AX6MeZ7GaOU4DbOO1JMb0bpqXm-W9Uc0WAXIBOfCD-u3SO1i8FahcHlQQQcPKIEzpdSD9TbLXB3_Ekn05dMql75P4NXMS-OkD4-LB0a6BE-ux9Pi29s3X9fvy83ndx_Wq02pKtaMpezBtHXT9gqMUYbiSrOuMVxKxWro-05TylqjFK6xhs5ozUnfVg3rOs7rrmanxauj7n7qB9Dq6FLsox1kPIggrbhb8XYntuFCVC2ta06ywItrgRiyhTSKwSYFzkkPYUqCdITTtms6nKHP_4Kehyn6bC-jaL4h46z9g9pKByJ_VcjnqllUrHiXlShrWEad_QOVu4b8Dfmxjc37dwjkSFAxpBTB3HgkWMyxE8fYiRw7McdOHDLn2e3HuWH8zlkG0CMg5ZLfQrzl6L-qvwAVeueV</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Zhang, Yong</creator><creator>Liang, Yupu</creator><creator>Levran, Orna</creator><creator>Randesi, Matthew</creator><creator>Yuferov, Vadim</creator><creator>Zhao, Connie</creator><creator>Kreek, Mary Jeanne</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170801</creationdate><title>Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study</title><author>Zhang, Yong ; Liang, Yupu ; Levran, Orna ; Randesi, Matthew ; Yuferov, Vadim ; Zhao, Connie ; Kreek, Mary Jeanne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-abef8678bceffcf205d397f4aac36ebb9d2238fcc060de9fdd41b857399446963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brain</topic><topic>Caudate-putamen</topic><topic>Complications and side effects</topic><topic>Dopamine</topic><topic>Dopamine receptors</topic><topic>Drug abuse</topic><topic>Drug interactions</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Immune response</topic><topic>Inflammation</topic><topic>Mesolimbic system</topic><topic>Methods</topic><topic>Mice</topic><topic>Narcotics</topic><topic>Neostriatum</topic><topic>Neurosciences</topic><topic>Opioid receptors (type mu)</topic><topic>Original Investigation</topic><topic>Oxycodone</topic><topic>Pharmacology/Toxicology</topic><topic>Psychiatry</topic><topic>Revisions</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA sequencing</topic><topic>Rodents</topic><topic>Self-administration</topic><topic>Striatum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yong</creatorcontrib><creatorcontrib>Liang, Yupu</creatorcontrib><creatorcontrib>Levran, Orna</creatorcontrib><creatorcontrib>Randesi, Matthew</creatorcontrib><creatorcontrib>Yuferov, Vadim</creatorcontrib><creatorcontrib>Zhao, Connie</creatorcontrib><creatorcontrib>Kreek, Mary Jeanne</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Psychopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yong</au><au>Liang, Yupu</au><au>Levran, Orna</au><au>Randesi, Matthew</au><au>Yuferov, Vadim</au><au>Zhao, Connie</au><au>Kreek, Mary Jeanne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study</atitle><jtitle>Psychopharmacology</jtitle><stitle>Psychopharmacology</stitle><addtitle>Psychopharmacology (Berl)</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>234</volume><issue>15</issue><spage>2259</spage><epage>2275</epage><pages>2259-2275</pages><issn>0033-3158</issn><eissn>1432-2072</eissn><abstract>Introduction
Non-medical use of prescription opioids such as the mu opioid receptor (MOP-r) agonist oxycodone is a growing problem in the USA and elsewhere. There is limited information about oxycodone’s impact on diverse gene systems in the brain.
Objectives
The current study was designed to examine how chronic oxycodone self-administration (SA) affects gene expression in the terminal areas of the nigrostriatal and mesolimbic dopaminergic pathways in mice.
Method
Adult male C57BL/6J mice underwent a 14-day oxycodone self-administration procedure (4 h/day, 0.25 mg/kg/infusion, FR1) and were euthanized 1 h after the last session. The dorsal and ventral striata were dissected, and total RNAs were extracted. Gene expressions were examined using RNA sequencing.
Result
We found that oxycodone self-administration exposure led to alterations of expression in numerous genes related to inflammation/immune functions in the dorsal striatum (54 upregulated genes and 1 downregulated gene) and ventral striatum (126 upregulated genes and 15 downregulated genes), with 38 upregulated genes identified in both brain regions.
Conclusion
This study reveals novel neurobiological mechanisms underlying some of the effects of a commonly abused prescription opioid. We propose that inflammation/immune gene systems may undergo a major change during chronic self-administration of oxycodone.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28653080</pmid><doi>10.1007/s00213-017-4657-y</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomedical and Life Sciences Biomedicine Brain Caudate-putamen Complications and side effects Dopamine Dopamine receptors Drug abuse Drug interactions Gene expression Genes Genetic aspects Health aspects Immune response Inflammation Mesolimbic system Methods Mice Narcotics Neostriatum Neurosciences Opioid receptors (type mu) Original Investigation Oxycodone Pharmacology/Toxicology Psychiatry Revisions Ribonucleic acid RNA RNA sequencing Rodents Self-administration Striatum |
| title | Alterations of expression of inflammation/immune-related genes in the dorsal and ventral striatum of adult C57BL/6J mice following chronic oxycodone self-administration: a RNA sequencing study |
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