Accurate Rapid Lifetime Determination on Time-Gated FLIM Microscopy with Optical Sectioning

Time-gated fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to assess the biochemistry of cells and tissues. When applied to living thick samples, it is hampered by the lack of optical sectioning and the need of acquiring many images for an accurate measurement of fluorescence...

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Veröffentlicht in:	Journal of healthcare engineering 2018-01, Vol.2018 (2018), p.1-7
Hauptverfasser:	Silva, Susana F., Morgado, António Miguel, Domingues, José Paulo
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Evaluation Fluorescence microscopy HeLa Cells Humans Image Processing, Computer-Assisted - methods Lasers Microscopy, Fluorescence - methods
Online-Zugang:	Volltext
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