Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, β-catenin and ZEB2 in laryngeal squamous cell carcinoma
Epithelial-mesenchymal transition (EMT) allows neoplastic cells to gain the invasive phenotype and become migratory, which is required for cancer progression and metastasis. In the present study, the expression of EMT-associated biomarkers and their association with clinicopathological parameters in...
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| Veröffentlicht in: | Oncology letters 2018-03, Vol.15 (3), p.3472-3481 |
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| creator | Zhu, Guang-Jie Song, Pan-Pan Zhou, Han Shen, Xiao-Hui Wang, Jun-Guo Ma, Xiao-Feng Gu, Ya-Jun Liu, Ding-Ding Feng, An-Ning Qian, Xiao-Yun Gao, Xia |
| description | Epithelial-mesenchymal transition (EMT) allows neoplastic cells to gain the invasive phenotype and become migratory, which is required for cancer progression and metastasis. In the present study, the expression of EMT-associated biomarkers and their association with clinicopathological parameters in laryngeal squamous cell carcinoma (LSCC) was investigated. E-cadherin, N-cadherin, β-catenin and zinc finger E-box binding homeobox 2 (ZEB2) protein expression was evaluated with immunohistochemistry in a cohort of 76 patients with operable LSCC. The association between these transition markers, clinicopathological parameters and their prognostic impact in LSCC was analyzed. Immunohistochemical analysis revealed that EMT-associated proteins were differentially expressed between LSCC and adjacent non-neoplastic laryngeal tissue. Negative E-cadherin expression and positive N-cadherin, β-catenin and ZEB2 expression were associated with a later tumor (T) stage, decreasing tumor differentiation and a reduced overall survival (OS) time (OS: E-cadherin, P=0.016; N-cadherin, P=0.003; β-catenin, P=0.002; ZEB2, P=0.0003). E-cadherin/β-catenin co-expression was significantly associated with the majority of clinicopathological parameters assessed, including lymph node metastases, T stage and tumor cell differentiation (P=0.004, P=0.005, and P |
| doi_str_mv | 10.3892/ol.2018.7751 |
| format | Article |
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In the present study, the expression of EMT-associated biomarkers and their association with clinicopathological parameters in laryngeal squamous cell carcinoma (LSCC) was investigated. E-cadherin, N-cadherin, β-catenin and zinc finger E-box binding homeobox 2 (ZEB2) protein expression was evaluated with immunohistochemistry in a cohort of 76 patients with operable LSCC. The association between these transition markers, clinicopathological parameters and their prognostic impact in LSCC was analyzed. Immunohistochemical analysis revealed that EMT-associated proteins were differentially expressed between LSCC and adjacent non-neoplastic laryngeal tissue. Negative E-cadherin expression and positive N-cadherin, β-catenin and ZEB2 expression were associated with a later tumor (T) stage, decreasing tumor differentiation and a reduced overall survival (OS) time (OS: E-cadherin, P=0.016; N-cadherin, P=0.003; β-catenin, P=0.002; ZEB2, P=0.0003). E-cadherin/β-catenin co-expression was significantly associated with the majority of clinicopathological parameters assessed, including lymph node metastases, T stage and tumor cell differentiation (P=0.004, P=0.005, and P<0.001, respectively). Multivariate analysis indicated that T stage and the positive expression of β-catenin and ZEB2 were independent risk factors for OS in LSCC (P=0.014, P=0.025 and P=0.003, respectively). It was concluded that EMT mediates tumor progression, and reduces OS time in patients with LSCC. E-cadherin/β-catenin co-expression may be associated with clinicopathological parameters. T stage, and the positive co-expression of β-catenin and ZEB2 may be independent predictors of prognosis in LSCC.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2018.7751</identifier><identifier>PMID: 29467869</identifier><language>eng</language><publisher>Greece: Spandidos Publications UK Ltd</publisher><subject>Biomarkers ; Cancer ; Cell adhesion & migration ; Laryngeal cancer ; Localization ; Lymphatic system ; Medical prognosis ; Metastasis ; Oncology ; Squamous cell carcinoma ; Statistical analysis</subject><ispartof>Oncology letters, 2018-03, Vol.15 (3), p.3472-3481</ispartof><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright: © Zhu et al. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-852760789b807b2c7a54436ad7ad1a135a604381e6f39adf2cf77101235727f93</citedby><cites>FETCH-LOGICAL-c412t-852760789b807b2c7a54436ad7ad1a135a604381e6f39adf2cf77101235727f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796309/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796309/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29467869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Guang-Jie</creatorcontrib><creatorcontrib>Song, Pan-Pan</creatorcontrib><creatorcontrib>Zhou, Han</creatorcontrib><creatorcontrib>Shen, Xiao-Hui</creatorcontrib><creatorcontrib>Wang, Jun-Guo</creatorcontrib><creatorcontrib>Ma, Xiao-Feng</creatorcontrib><creatorcontrib>Gu, Ya-Jun</creatorcontrib><creatorcontrib>Liu, Ding-Ding</creatorcontrib><creatorcontrib>Feng, An-Ning</creatorcontrib><creatorcontrib>Qian, Xiao-Yun</creatorcontrib><creatorcontrib>Gao, Xia</creatorcontrib><title>Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, β-catenin and ZEB2 in laryngeal squamous cell carcinoma</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>Epithelial-mesenchymal transition (EMT) allows neoplastic cells to gain the invasive phenotype and become migratory, which is required for cancer progression and metastasis. In the present study, the expression of EMT-associated biomarkers and their association with clinicopathological parameters in laryngeal squamous cell carcinoma (LSCC) was investigated. E-cadherin, N-cadherin, β-catenin and zinc finger E-box binding homeobox 2 (ZEB2) protein expression was evaluated with immunohistochemistry in a cohort of 76 patients with operable LSCC. The association between these transition markers, clinicopathological parameters and their prognostic impact in LSCC was analyzed. Immunohistochemical analysis revealed that EMT-associated proteins were differentially expressed between LSCC and adjacent non-neoplastic laryngeal tissue. Negative E-cadherin expression and positive N-cadherin, β-catenin and ZEB2 expression were associated with a later tumor (T) stage, decreasing tumor differentiation and a reduced overall survival (OS) time (OS: E-cadherin, P=0.016; N-cadherin, P=0.003; β-catenin, P=0.002; ZEB2, P=0.0003). E-cadherin/β-catenin co-expression was significantly associated with the majority of clinicopathological parameters assessed, including lymph node metastases, T stage and tumor cell differentiation (P=0.004, P=0.005, and P<0.001, respectively). Multivariate analysis indicated that T stage and the positive expression of β-catenin and ZEB2 were independent risk factors for OS in LSCC (P=0.014, P=0.025 and P=0.003, respectively). It was concluded that EMT mediates tumor progression, and reduces OS time in patients with LSCC. E-cadherin/β-catenin co-expression may be associated with clinicopathological parameters. T stage, and the positive co-expression of β-catenin and ZEB2 may be independent predictors of prognosis in LSCC.</description><subject>Biomarkers</subject><subject>Cancer</subject><subject>Cell adhesion & migration</subject><subject>Laryngeal cancer</subject><subject>Localization</subject><subject>Lymphatic system</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Oncology</subject><subject>Squamous cell carcinoma</subject><subject>Statistical analysis</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkc1q3DAUhUVoSUKaXddBkE0X8VQ_tiRvAm2Y_kBoILSbbsQdWc4okaWJZBdmm0fKg_SZKpN0SKuNzkUfh3t0EHpLyYKrlr2PfsEIVQspG7qHDqlsWUWJYq92WtYH6DjnW1JOI6hSYh8dsLYWUon2ED1cR29x7LHduHFtvQNfDTbbYNbbATweE4TsRhcDHiDd2ZTxsjLQrW1y4Qx_e6F_P5ZhtMEFDKHDP5cfGS7aQ9qGG1u88v0EQ5wyNtZ7bCAZF-IAb9DrHny2x8_3Efrxafn94kt1efX568WHy8rUlI2VapgURKp2pYhcMSOhqWsuoJPQUaC8AUFqrqgVPW-h65nppaSEMt5IJvuWH6HzJ9_NtBpsZ2wo4bzeJFeSbXUEp_99CW6tb-Iv3chWcDIbvHs2SPF-snnUg8tzFgi2xNKMlN9mRFFR0NP_0Ns4pVDiFYrWtGwkZaHOniiTYs7J9rtlKNFzvzr6mVd67rfgJy8D7OC_bfI_F_ShpA</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Zhu, Guang-Jie</creator><creator>Song, Pan-Pan</creator><creator>Zhou, Han</creator><creator>Shen, Xiao-Hui</creator><creator>Wang, Jun-Guo</creator><creator>Ma, Xiao-Feng</creator><creator>Gu, Ya-Jun</creator><creator>Liu, Ding-Ding</creator><creator>Feng, An-Ning</creator><creator>Qian, Xiao-Yun</creator><creator>Gao, Xia</creator><general>Spandidos Publications UK Ltd</general><general>D.A. 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In the present study, the expression of EMT-associated biomarkers and their association with clinicopathological parameters in laryngeal squamous cell carcinoma (LSCC) was investigated. E-cadherin, N-cadherin, β-catenin and zinc finger E-box binding homeobox 2 (ZEB2) protein expression was evaluated with immunohistochemistry in a cohort of 76 patients with operable LSCC. The association between these transition markers, clinicopathological parameters and their prognostic impact in LSCC was analyzed. Immunohistochemical analysis revealed that EMT-associated proteins were differentially expressed between LSCC and adjacent non-neoplastic laryngeal tissue. Negative E-cadherin expression and positive N-cadherin, β-catenin and ZEB2 expression were associated with a later tumor (T) stage, decreasing tumor differentiation and a reduced overall survival (OS) time (OS: E-cadherin, P=0.016; N-cadherin, P=0.003; β-catenin, P=0.002; ZEB2, P=0.0003). E-cadherin/β-catenin co-expression was significantly associated with the majority of clinicopathological parameters assessed, including lymph node metastases, T stage and tumor cell differentiation (P=0.004, P=0.005, and P<0.001, respectively). Multivariate analysis indicated that T stage and the positive expression of β-catenin and ZEB2 were independent risk factors for OS in LSCC (P=0.014, P=0.025 and P=0.003, respectively). It was concluded that EMT mediates tumor progression, and reduces OS time in patients with LSCC. E-cadherin/β-catenin co-expression may be associated with clinicopathological parameters. T stage, and the positive co-expression of β-catenin and ZEB2 may be independent predictors of prognosis in LSCC.</abstract><cop>Greece</cop><pub>Spandidos Publications UK Ltd</pub><pmid>29467869</pmid><doi>10.3892/ol.2018.7751</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Biomarkers Cancer Cell adhesion & migration Laryngeal cancer Localization Lymphatic system Medical prognosis Metastasis Oncology Squamous cell carcinoma Statistical analysis |
| title | Role of epithelial-mesenchymal transition markers E-cadherin, N-cadherin, β-catenin and ZEB2 in laryngeal squamous cell carcinoma |
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