Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes
In this study, we have characterized a novel set of extracellular enzymes produced by Penicillium chrysogenum strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have...
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| Veröffentlicht in: | 3 Biotech 2018-02, Vol.8 (2), p.106-5, Article 106 |
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| creator | Gujar, Vaibhav V. Fuke, Priya Khardenavis, Anshuman A. Purohit, Hemant J. |
| description | In this study, we have characterized a novel set of extracellular enzymes produced by
Penicillium chrysogenum
strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with
Penicillium rubens, Penicillium chrysogenum
followed by
Penicillium expansum
and
Penicillium roqueforti
. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes. |
| doi_str_mv | 10.1007/s13205-018-1132-3 |
| format | Article |
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Penicillium chrysogenum
strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with
Penicillium rubens, Penicillium chrysogenum
followed by
Penicillium expansum
and
Penicillium roqueforti
. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.</description><identifier>ISSN: 2190-572X</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-018-1132-3</identifier><identifier>PMID: 29430367</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Agriculture ; Annotations ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer Research ; Chemistry ; Chemistry and Materials Science ; Enzymes ; Extracellular enzymes ; Fungi ; Genes ; Genomes ; Homology ; Inulinase ; Invertase ; Nucleotide sequence ; Oligosaccharides ; Penicillin ; Penicillium chrysogenum ; Prebiotics ; Short Reports ; Stem Cells ; Synthesis</subject><ispartof>3 Biotech, 2018-02, Vol.8 (2), p.106-5, Article 106</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>3 Biotech is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-d4d5a18f688598cb10c78178ac5f0442a401941d36c17d6651af5231e54270f33</citedby><cites>FETCH-LOGICAL-c437t-d4d5a18f688598cb10c78178ac5f0442a401941d36c17d6651af5231e54270f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794679/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5794679/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29430367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gujar, Vaibhav V.</creatorcontrib><creatorcontrib>Fuke, Priya</creatorcontrib><creatorcontrib>Khardenavis, Anshuman A.</creatorcontrib><creatorcontrib>Purohit, Hemant J.</creatorcontrib><title>Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>In this study, we have characterized a novel set of extracellular enzymes produced by
Penicillium chrysogenum
strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with
Penicillium rubens, Penicillium chrysogenum
followed by
Penicillium expansum
and
Penicillium roqueforti
. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.</description><subject>Agriculture</subject><subject>Annotations</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzymes</subject><subject>Extracellular enzymes</subject><subject>Fungi</subject><subject>Genes</subject><subject>Genomes</subject><subject>Homology</subject><subject>Inulinase</subject><subject>Invertase</subject><subject>Nucleotide sequence</subject><subject>Oligosaccharides</subject><subject>Penicillin</subject><subject>Penicillium chrysogenum</subject><subject>Prebiotics</subject><subject>Short Reports</subject><subject>Stem Cells</subject><subject>Synthesis</subject><issn>2190-572X</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UcFu1DAQtRCIVqUfwAVZ4krAY8dxfEFCLaWISnAAiZvldeysq8QOtgPafgDfjVdbVuXAXOZJ8-bN0zyEngN5DYSINxkYJbwh0DdQYcMeoVMKkjRcsP7xEdPvJ-g851tSiwOXQJ6iEypbRlgnTtHvy6RdwaMNcbY42x-rDcbi6PAXG7zx0-TXGZtt2uVYSRXnkrQP-PrTFX2FNXZrGNeMf_myxUssNhSvJ-xiwkuKw2qKj2EvtyS78bF4g_MulK3N_s6HEdtwt5ttfoaeOD1le37fz9C3q_dfL66bm88fPl68u2lMy0RphnbgGnrX9T2XvdkAMaIH0WvDHWlbqlsCsoWBdQbE0HUctOOUgeUtFcQxdobeHnSXdTPbwVS7SU9qSX7Waaei9urfSfBbNcafigvZdkJWgZf3AinWX-WibuOaQvWsQErW1a_SrrLgwDIp5pysO14AovbpqUN6qqan9umpvbUXD60dN_5mVQn0QMh1FEabHpz-r-of3jWneg</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Gujar, Vaibhav V.</creator><creator>Fuke, Priya</creator><creator>Khardenavis, Anshuman A.</creator><creator>Purohit, Hemant J.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>5PM</scope></search><sort><creationdate>20180201</creationdate><title>Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes</title><author>Gujar, Vaibhav V. ; Fuke, Priya ; Khardenavis, Anshuman A. ; Purohit, Hemant J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-d4d5a18f688598cb10c78178ac5f0442a401941d36c17d6651af5231e54270f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Agriculture</topic><topic>Annotations</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Enzymes</topic><topic>Extracellular enzymes</topic><topic>Fungi</topic><topic>Genes</topic><topic>Genomes</topic><topic>Homology</topic><topic>Inulinase</topic><topic>Invertase</topic><topic>Nucleotide sequence</topic><topic>Oligosaccharides</topic><topic>Penicillin</topic><topic>Penicillium chrysogenum</topic><topic>Prebiotics</topic><topic>Short Reports</topic><topic>Stem Cells</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gujar, Vaibhav V.</creatorcontrib><creatorcontrib>Fuke, Priya</creatorcontrib><creatorcontrib>Khardenavis, Anshuman A.</creatorcontrib><creatorcontrib>Purohit, Hemant J.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gujar, Vaibhav V.</au><au>Fuke, Priya</au><au>Khardenavis, Anshuman A.</au><au>Purohit, Hemant J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>8</volume><issue>2</issue><spage>106</spage><epage>5</epage><pages>106-5</pages><artnum>106</artnum><issn>2190-572X</issn><eissn>2190-5738</eissn><abstract>In this study, we have characterized a novel set of extracellular enzymes produced by
Penicillium chrysogenum
strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with
Penicillium rubens, Penicillium chrysogenum
followed by
Penicillium expansum
and
Penicillium roqueforti
. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29430367</pmid><doi>10.1007/s13205-018-1132-3</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Annotations Bioinformatics Biomaterials Biotechnology Cancer Research Chemistry Chemistry and Materials Science Enzymes Extracellular enzymes Fungi Genes Genomes Homology Inulinase Invertase Nucleotide sequence Oligosaccharides Penicillin Penicillium chrysogenum Prebiotics Short Reports Stem Cells Synthesis |
| title | Draft genome sequence of Penicillium chrysogenum strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes |
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