Production of human vitronectin in Nicotiana benthamiana using the INPACT hyperexpression platform

Summary Human vitronectin (hVN) is a glycoprotein that functions as a cell adhesion molecule and a regulator of coagulation in blood plasma and the extracellular matrix. In vitro, hVN is added to serum‐free media in order to promote the adhesion of animal cells to tissue culture surfaces and the pro...

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Veröffentlicht in:	Plant biotechnology journal 2018-02, Vol.16 (2), p.394-403
Hauptverfasser:	Dugdale, Benjamin, Kato, Maiko, Deo, Pradeep, Plan, Manuel, Harrison, Mark, Lloyd, Robyn, Walsh, Terry, Harding, Robert, Dale, James
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesion biopharming Blood coagulation Blood plasma Bushy stunt Cell adhesion Cell adhesion & migration Cell adhesion molecules Cell culture Cell proliferation coagulation Culture media Dilution Ethanol Extracellular matrix fibroblasts filtration Gene expression Gene Expression Regulation, Plant genes genetic transformation Glycoproteins Heparin Humans inducible INPACT mice Nicotiana - genetics Nicotiana - metabolism Nicotiana benthamiana Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Polyhistidine Proteins recombinant proteins Serum-free medium Stem cell transplantation Stem cells Storage conditions Tissue culture Tobacco Tobacco yellow dwarf virus Tomato bushy stunt virus Tomatoes Transcription transcription (genetics) transgene expression Viruses Vitronectin Vitronectin - genetics Vitronectin - metabolism
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