CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum
Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of gen...
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| Veröffentlicht in: | Scientific reports 2018-01, Vol.8 (1), p.1426-11, Article 1426 |
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| description | Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle,
Tribolium castaneum
and the
Tribolium
cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in
T. castaneum
larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both
T. castaneum
and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect,
T.castaneum
. |
| doi_str_mv | 10.1038/s41598-018-19667-6 |
| format | Article |
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Tribolium castaneum
and the
Tribolium
cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in
T. castaneum
larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both
T. castaneum
and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect,
T.castaneum
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Tribolium castaneum
and the
Tribolium
cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in
T. castaneum
larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both
T. castaneum
and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect,
T.castaneum
.</description><subject>38</subject><subject>38/1</subject><subject>38/89</subject><subject>38/90</subject><subject>631/601/1466</subject><subject>631/601/1737</subject><subject>82</subject><subject>82/29</subject><subject>Acetylation</subject><subject>Chromatin</subject><subject>CREB-binding protein</subject><subject>Cyclic AMP response element-binding protein</subject><subject>Ecdysteroids</subject><subject>Histone acetyltransferase</subject><subject>Histone deacetylase</subject><subject>Hormones</subject><subject>Humanities and Social Sciences</subject><subject>Immunoprecipitation</subject><subject>Insects</subject><subject>Juvenile hormones</subject><subject>Larvae</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Transcription activation</subject><subject>Transcription factors</subject><subject>Tribolium</subject><subject>Tribolium castaneum</subject><subject>Trichostatin A</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kV9rFDEUxYMottR-AR8k4IsPTs3_nbwIdqm2UBBK-xwykzu7WTPJmswU9tubdmtZheYlIed3T-7NQeg9JWeU8PZLEVTqtiG0bahWatGoV-iYESEbxhl7fXA-QqelbEhdkmlB9Vt0xDRXTFB1jMLy5uK86Xx0Pq7wNqcJfMTbYHcF_4IdzilAwfVqM99D9AHwOuUxRcC2n3yKD9K0BpzB4SGkOeMOYArwGd9m36Xg5xEvbZlshHl8h94MNhQ4fdpP0N33i9vlZXP988fV8tt100tKp4Z1kmuQSrHWMtoNFGirBq2Jk1J3esE5GYhw0g6a9YNgXCriBqKY0y1z4PgJ-rr33c7dCK6HOGUbzDb70eadSdabf5Xo12aV7o1ctEQwUg0-PRnk9HuGMpnRlx5CqGOkuRham2mlooJX9ON_6Kb-QqzjPVJiIRRnlWJ7qs-plAzDczOUmIc8zT5PU_M0j3kaVYs-HI7xXPI3vQrwPVCqFFeQD95-2fYPVKmrsg</recordid><startdate>20180123</startdate><enddate>20180123</enddate><creator>Xu, Jingjing</creator><creator>Roy, Amit</creator><creator>Palli, Subba Reddy</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3237-3525</orcidid><orcidid>https://orcid.org/0000-0002-0873-3247</orcidid></search><sort><creationdate>20180123</creationdate><title>CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum</title><author>Xu, Jingjing ; 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CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle,
Tribolium castaneum
and the
Tribolium
cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in
T. castaneum
larvae, adults and TcA cells whereas, Trichostatin A [TSA, a histone deacetylase (HDAC) inhibitor] induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both
T. castaneum
and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the importance of CBP-mediated acetylation of H3K27 for JH induction of Kr-h1, 4EBP, and G13402 in TcA cells. These data suggest that CBP plays an important role in JH action in the model insect,
T.castaneum
.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29362416</pmid><doi>10.1038/s41598-018-19667-6</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3237-3525</orcidid><orcidid>https://orcid.org/0000-0002-0873-3247</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 38 38/1 38/89 38/90 631/601/1466 631/601/1737 82 82/29 Acetylation Chromatin CREB-binding protein Cyclic AMP response element-binding protein Ecdysteroids Histone acetyltransferase Histone deacetylase Hormones Humanities and Social Sciences Immunoprecipitation Insects Juvenile hormones Larvae multidisciplinary Science Science (multidisciplinary) Transcription activation Transcription factors Tribolium Tribolium castaneum Trichostatin A |
| title | CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum |
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