Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells
Cancer prevention using natural micronutrition on epigenetic mechanisms primarily revolves around plant extracts. However, the role of macronutrition, including animal peptides, on epigenetic modification in cancer has been elusive. In traditional Chinese medicine, the soft-shelled turtle has a long...
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| Veröffentlicht in: | Oncology letters 2018-03, Vol.15 (3), p.3109-3120 |
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| creator | Wu, Yi-Chen Liu, Xiang Wang, Jiu-Li Chen, Xiang-Liu Lei, Lan Han, Jing Jiang, You-Shui Ling, Zhi-Qiang |
| description | Cancer prevention using natural micronutrition on epigenetic mechanisms primarily revolves around plant extracts. However, the role of macronutrition, including animal peptides, on epigenetic modification in cancer has been elusive. In traditional Chinese medicine, the soft-shelled turtle has a long-history of being a functional food that strengthens immunity through unknown mechanisms. The present study aimed to investigate the impact of soft-shelled turtle peptide on microRNA (miRNA) expression in gastric cancer (GC) cells and to analyze the potential anticancer mechanisms for GC. Affymetrix GeneChip miRNA 3.0 Array and quantitative polymerase chain reaction were used to detect the miRNA expression profile in human GC AGS cells treated with the soft-shelled turtle peptide. The results demonstrated that 101 miRNAs (49 upregulated miRNAs and 52 downregulated miRNAs) were significantly differentially expressed in the AGS cells following soft-shelled turtle peptide treatment. Several tumor suppressor miRNAs were upregulated markedly, including miRNA-375, let-7d, miRNA-429, miRNA-148a/148b and miRNA-34a. Pathway analysis indicated that soft-shelled turtle peptide may function with anticancer properties through the Hippo signaling pathway and the forkhead box O signaling pathway. Therefore, these results demonstrated that soft-shelled turtle peptide has the capacity to influence cancer-related pathways through the regulation of miRNA expression in GC cells. |
| doi_str_mv | 10.3892/ol.2017.7692 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5778892</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A533095398</galeid><sourcerecordid>A533095398</sourcerecordid><originalsourceid>FETCH-LOGICAL-c510t-b8287d444a59795e2903a2fab4234bf5ca23a3abc0228de7106afe734e8d42013</originalsourceid><addsrcrecordid>eNptks1rFTEUxQdRbKnduZYBQbpwnvl8STbCo2gVioJVcBcyM3fepGSSMckI_vdmaH32ickigfzuSc7JrarnGG2oVORNcBuCsNiIrSKPqlMsFGkwkuTxYS_YSXWe0i0qg2-xlNun1QlRjHLE2Gn1_SYMuUkjOAd9nZeYHdQzzNn2UE-hX5zJkOrJdjF8-bSr5xgGWxDr63GZjK_3JuVou7ozvoNY765u6q6IpWfVk8G4BOf361n17f27r5cfmuvPVx8vd9dNxzHKTSuJFD1jzHAlFAeiEDVkMC0jlLUD7wyhhpq2Q4TIHgRGWzOAoAxkz4p1ela9vdOdl3aCvgOfo3F6jnYy8ZcOxurjE29HvQ8_NRdClgiLwMW9QAw_FkhZTzatFoyHsCRNEMIKc8l5QV_-g96GJfpir1CYUUoFxX-pvXGgrR9CubdbRfWOU4oUp0oWavMfqsweStjBwxrzccGrBwUjGJfHFNySbfDpGHx9B5YvSynCcAgDI712jQ5ufa_Qa9cU_MXDAA_wnx6hvwEfnLqQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2014333731</pqid></control><display><type>article</type><title>Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells</title><source>Spandidos Publications Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Wu, Yi-Chen ; Liu, Xiang ; Wang, Jiu-Li ; Chen, Xiang-Liu ; Lei, Lan ; Han, Jing ; Jiang, You-Shui ; Ling, Zhi-Qiang</creator><creatorcontrib>Wu, Yi-Chen ; Liu, Xiang ; Wang, Jiu-Li ; Chen, Xiang-Liu ; Lei, Lan ; Han, Jing ; Jiang, You-Shui ; Ling, Zhi-Qiang</creatorcontrib><description>Cancer prevention using natural micronutrition on epigenetic mechanisms primarily revolves around plant extracts. However, the role of macronutrition, including animal peptides, on epigenetic modification in cancer has been elusive. In traditional Chinese medicine, the soft-shelled turtle has a long-history of being a functional food that strengthens immunity through unknown mechanisms. The present study aimed to investigate the impact of soft-shelled turtle peptide on microRNA (miRNA) expression in gastric cancer (GC) cells and to analyze the potential anticancer mechanisms for GC. Affymetrix GeneChip miRNA 3.0 Array and quantitative polymerase chain reaction were used to detect the miRNA expression profile in human GC AGS cells treated with the soft-shelled turtle peptide. The results demonstrated that 101 miRNAs (49 upregulated miRNAs and 52 downregulated miRNAs) were significantly differentially expressed in the AGS cells following soft-shelled turtle peptide treatment. Several tumor suppressor miRNAs were upregulated markedly, including miRNA-375, let-7d, miRNA-429, miRNA-148a/148b and miRNA-34a. Pathway analysis indicated that soft-shelled turtle peptide may function with anticancer properties through the Hippo signaling pathway and the forkhead box O signaling pathway. Therefore, these results demonstrated that soft-shelled turtle peptide has the capacity to influence cancer-related pathways through the regulation of miRNA expression in GC cells.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2017.7692</identifier><identifier>PMID: 29435044</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Angiotensin converting enzyme inhibitors ; Cancer genetics ; Cancer research ; Care and treatment ; Cell growth ; Chinese medicine ; Chinese softshell turtle ; Epigenetic inheritance ; Epigenetics ; Gastric cancer ; Health aspects ; Kinases ; MicroRNA ; Oncology ; Stomach cancer ; Traditional Chinese medicine</subject><ispartof>Oncology letters, 2018-03, Vol.15 (3), p.3109-3120</ispartof><rights>COPYRIGHT 2018 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright © 2018, Spandidos Publications 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-b8287d444a59795e2903a2fab4234bf5ca23a3abc0228de7106afe734e8d42013</citedby><cites>FETCH-LOGICAL-c510t-b8287d444a59795e2903a2fab4234bf5ca23a3abc0228de7106afe734e8d42013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778892/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778892/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29435044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Yi-Chen</creatorcontrib><creatorcontrib>Liu, Xiang</creatorcontrib><creatorcontrib>Wang, Jiu-Li</creatorcontrib><creatorcontrib>Chen, Xiang-Liu</creatorcontrib><creatorcontrib>Lei, Lan</creatorcontrib><creatorcontrib>Han, Jing</creatorcontrib><creatorcontrib>Jiang, You-Shui</creatorcontrib><creatorcontrib>Ling, Zhi-Qiang</creatorcontrib><title>Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>Cancer prevention using natural micronutrition on epigenetic mechanisms primarily revolves around plant extracts. However, the role of macronutrition, including animal peptides, on epigenetic modification in cancer has been elusive. In traditional Chinese medicine, the soft-shelled turtle has a long-history of being a functional food that strengthens immunity through unknown mechanisms. The present study aimed to investigate the impact of soft-shelled turtle peptide on microRNA (miRNA) expression in gastric cancer (GC) cells and to analyze the potential anticancer mechanisms for GC. Affymetrix GeneChip miRNA 3.0 Array and quantitative polymerase chain reaction were used to detect the miRNA expression profile in human GC AGS cells treated with the soft-shelled turtle peptide. The results demonstrated that 101 miRNAs (49 upregulated miRNAs and 52 downregulated miRNAs) were significantly differentially expressed in the AGS cells following soft-shelled turtle peptide treatment. Several tumor suppressor miRNAs were upregulated markedly, including miRNA-375, let-7d, miRNA-429, miRNA-148a/148b and miRNA-34a. Pathway analysis indicated that soft-shelled turtle peptide may function with anticancer properties through the Hippo signaling pathway and the forkhead box O signaling pathway. Therefore, these results demonstrated that soft-shelled turtle peptide has the capacity to influence cancer-related pathways through the regulation of miRNA expression in GC cells.</description><subject>Angiotensin converting enzyme inhibitors</subject><subject>Cancer genetics</subject><subject>Cancer research</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>Chinese medicine</subject><subject>Chinese softshell turtle</subject><subject>Epigenetic inheritance</subject><subject>Epigenetics</subject><subject>Gastric cancer</subject><subject>Health aspects</subject><subject>Kinases</subject><subject>MicroRNA</subject><subject>Oncology</subject><subject>Stomach cancer</subject><subject>Traditional Chinese medicine</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptks1rFTEUxQdRbKnduZYBQbpwnvl8STbCo2gVioJVcBcyM3fepGSSMckI_vdmaH32ickigfzuSc7JrarnGG2oVORNcBuCsNiIrSKPqlMsFGkwkuTxYS_YSXWe0i0qg2-xlNun1QlRjHLE2Gn1_SYMuUkjOAd9nZeYHdQzzNn2UE-hX5zJkOrJdjF8-bSr5xgGWxDr63GZjK_3JuVou7ozvoNY765u6q6IpWfVk8G4BOf361n17f27r5cfmuvPVx8vd9dNxzHKTSuJFD1jzHAlFAeiEDVkMC0jlLUD7wyhhpq2Q4TIHgRGWzOAoAxkz4p1ela9vdOdl3aCvgOfo3F6jnYy8ZcOxurjE29HvQ8_NRdClgiLwMW9QAw_FkhZTzatFoyHsCRNEMIKc8l5QV_-g96GJfpir1CYUUoFxX-pvXGgrR9CubdbRfWOU4oUp0oWavMfqsweStjBwxrzccGrBwUjGJfHFNySbfDpGHx9B5YvSynCcAgDI712jQ5ufa_Qa9cU_MXDAA_wnx6hvwEfnLqQ</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Wu, Yi-Chen</creator><creator>Liu, Xiang</creator><creator>Wang, Jiu-Li</creator><creator>Chen, Xiang-Liu</creator><creator>Lei, Lan</creator><creator>Han, Jing</creator><creator>Jiang, You-Shui</creator><creator>Ling, Zhi-Qiang</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. 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However, the role of macronutrition, including animal peptides, on epigenetic modification in cancer has been elusive. In traditional Chinese medicine, the soft-shelled turtle has a long-history of being a functional food that strengthens immunity through unknown mechanisms. The present study aimed to investigate the impact of soft-shelled turtle peptide on microRNA (miRNA) expression in gastric cancer (GC) cells and to analyze the potential anticancer mechanisms for GC. Affymetrix GeneChip miRNA 3.0 Array and quantitative polymerase chain reaction were used to detect the miRNA expression profile in human GC AGS cells treated with the soft-shelled turtle peptide. The results demonstrated that 101 miRNAs (49 upregulated miRNAs and 52 downregulated miRNAs) were significantly differentially expressed in the AGS cells following soft-shelled turtle peptide treatment. Several tumor suppressor miRNAs were upregulated markedly, including miRNA-375, let-7d, miRNA-429, miRNA-148a/148b and miRNA-34a. Pathway analysis indicated that soft-shelled turtle peptide may function with anticancer properties through the Hippo signaling pathway and the forkhead box O signaling pathway. Therefore, these results demonstrated that soft-shelled turtle peptide has the capacity to influence cancer-related pathways through the regulation of miRNA expression in GC cells.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>29435044</pmid><doi>10.3892/ol.2017.7692</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Angiotensin converting enzyme inhibitors Cancer genetics Cancer research Care and treatment Cell growth Chinese medicine Chinese softshell turtle Epigenetic inheritance Epigenetics Gastric cancer Health aspects Kinases MicroRNA Oncology Stomach cancer Traditional Chinese medicine |
| title | Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells |
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