Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells
Actin-related protein 2/3 complex subunit 4 (ARPC4) acts as an actin nucleator in actin cytoskeleton branching and contributes to cell migration. ARPC4 has previously been demonstrated to be abnormally expressed in various colorectal carcinoma cell lines, particularly SW620 cells. The present study...
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| Veröffentlicht in: | Oncology letters 2018-03, Vol.15 (3), p.2847-2854 |
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| creator | Su, Xiaojuan Wang, Siyang Huo, Yongxu Yang, Chunlei |
| description | Actin-related protein 2/3 complex subunit 4 (ARPC4) acts as an actin nucleator in actin cytoskeleton branching and contributes to cell migration. ARPC4 has previously been demonstrated to be abnormally expressed in various colorectal carcinoma cell lines, particularly SW620 cells. The present study explored the biological action and the possible mechanisms underlying the function of ARPC4 in the progression of carcinoma. The proliferation and migration of SW620 cells transfected with ARPC4-specific short interfering (si)RNAs were assessed using western blot, cell counting, flow cytometry and transwell assays. SW620 cells exhibited the highest ARPC4 expression of the cell lines investigated, and siRNA538 was the most effective of the siRNAs considered. The results of the present study demonstrated that ARPC4-silencing exhibited a significant effect on the capacity of cells for migration, but did not affect their proliferative ability. ARPC4-silencing inhibited human SW620 cell migration, but not proliferation,
, suggesting that ARPC4 may be a putative therapeutic target for colorectal carcinoma. |
| doi_str_mv | 10.3892/ol.2017.7642 |
| format | Article |
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, suggesting that ARPC4 may be a putative therapeutic target for colorectal carcinoma.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2017.7642</identifier><identifier>PMID: 29435011</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Actin ; Binding proteins ; Biology ; Care and treatment ; Cell adhesion & migration ; Cell culture ; Cell growth ; Colorectal cancer ; Development and progression ; Gene therapy ; Genetic aspects ; Health aspects ; Immunoglobulins ; Medical research ; Metastasis ; Mortality ; Protein expression ; Proteins ; Tumors</subject><ispartof>Oncology letters, 2018-03, Vol.15 (3), p.2847-2854</ispartof><rights>COPYRIGHT 2018 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2018</rights><rights>Copyright: © Su et al. 2018</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-b736a5fef68746c8493244dc5a97b292560fa7f808094ed228161561768f52dd3</citedby><cites>FETCH-LOGICAL-c510t-b736a5fef68746c8493244dc5a97b292560fa7f808094ed228161561768f52dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778834/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5778834/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29435011$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Xiaojuan</creatorcontrib><creatorcontrib>Wang, Siyang</creatorcontrib><creatorcontrib>Huo, Yongxu</creatorcontrib><creatorcontrib>Yang, Chunlei</creatorcontrib><title>Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>Actin-related protein 2/3 complex subunit 4 (ARPC4) acts as an actin nucleator in actin cytoskeleton branching and contributes to cell migration. ARPC4 has previously been demonstrated to be abnormally expressed in various colorectal carcinoma cell lines, particularly SW620 cells. The present study explored the biological action and the possible mechanisms underlying the function of ARPC4 in the progression of carcinoma. The proliferation and migration of SW620 cells transfected with ARPC4-specific short interfering (si)RNAs were assessed using western blot, cell counting, flow cytometry and transwell assays. SW620 cells exhibited the highest ARPC4 expression of the cell lines investigated, and siRNA538 was the most effective of the siRNAs considered. The results of the present study demonstrated that ARPC4-silencing exhibited a significant effect on the capacity of cells for migration, but did not affect their proliferative ability. ARPC4-silencing inhibited human SW620 cell migration, but not proliferation,
, suggesting that ARPC4 may be a putative therapeutic target for colorectal carcinoma.</description><subject>Actin</subject><subject>Binding proteins</subject><subject>Biology</subject><subject>Care and treatment</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Colorectal cancer</subject><subject>Development and progression</subject><subject>Gene therapy</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Immunoglobulins</subject><subject>Medical research</subject><subject>Metastasis</subject><subject>Mortality</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Tumors</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkltrFDEUxwdRbKl981kCgvjgbnOdZF6EpXiDomAVH0M2c2YnJZOsSUb0g_h9zdi6tmLykEPO7_yTc2maxwSvmeroWfRriolcy5bTe80xkR1dEazo_YMt-VFzmvMVrku0RKn2YXNEO84EJuS4-Xk5xlSQCwXSAMmFHfr4frOaoHemQI-y8xDsch0HZGxxYZXA_3btUyzgAqJnDNk47T18R3nezsEVxKvi6LauZFRGQJPbJVNcDIvK5ZeWYjTOkwk1zscEthiPrAkWErLgfX7UPBiMz3B6c540n1-_-nT-dnXx4c27883FygqCy2orWWvEAEOrJG-t4h2jnPdWmE5uaUdFiwcjB4UV7jj0lCrSkloE2apB0L5nJ83La939vK0pWwglGa_3yU0m_dDROH3XE9yod_GbFlIqxXgVeH4jkOLXGXLRk8tLCiZAnLOmGJOOiE6yij79B72Kcwo1vUoRzpjgHf5L7YwH7cIQ67t2EdUbwRjuBGsXrfV_qLp7mJyNAYbatrsBz24FjGB8GXP089KTfBd8cQ3aFHNOMByKQbBeRk5Hv_xX6mXkKv7kdgEP8J8BY78AcYXPbw</recordid><startdate>20180301</startdate><enddate>20180301</enddate><creator>Su, Xiaojuan</creator><creator>Wang, Siyang</creator><creator>Huo, Yongxu</creator><creator>Yang, Chunlei</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180301</creationdate><title>Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells</title><author>Su, Xiaojuan ; Wang, Siyang ; Huo, Yongxu ; Yang, Chunlei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-b736a5fef68746c8493244dc5a97b292560fa7f808094ed228161561768f52dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Actin</topic><topic>Binding proteins</topic><topic>Biology</topic><topic>Care and treatment</topic><topic>Cell adhesion & migration</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Colorectal cancer</topic><topic>Development and progression</topic><topic>Gene therapy</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Immunoglobulins</topic><topic>Medical research</topic><topic>Metastasis</topic><topic>Mortality</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>Su, Xiaojuan</creatorcontrib><creatorcontrib>Wang, Siyang</creatorcontrib><creatorcontrib>Huo, Yongxu</creatorcontrib><creatorcontrib>Yang, Chunlei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Xiaojuan</au><au>Wang, Siyang</au><au>Huo, Yongxu</au><au>Yang, Chunlei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>15</volume><issue>3</issue><spage>2847</spage><epage>2854</epage><pages>2847-2854</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>Actin-related protein 2/3 complex subunit 4 (ARPC4) acts as an actin nucleator in actin cytoskeleton branching and contributes to cell migration. ARPC4 has previously been demonstrated to be abnormally expressed in various colorectal carcinoma cell lines, particularly SW620 cells. The present study explored the biological action and the possible mechanisms underlying the function of ARPC4 in the progression of carcinoma. The proliferation and migration of SW620 cells transfected with ARPC4-specific short interfering (si)RNAs were assessed using western blot, cell counting, flow cytometry and transwell assays. SW620 cells exhibited the highest ARPC4 expression of the cell lines investigated, and siRNA538 was the most effective of the siRNAs considered. The results of the present study demonstrated that ARPC4-silencing exhibited a significant effect on the capacity of cells for migration, but did not affect their proliferative ability. ARPC4-silencing inhibited human SW620 cell migration, but not proliferation,
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| source | Spandidos Publications Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Actin Binding proteins Biology Care and treatment Cell adhesion & migration Cell culture Cell growth Colorectal cancer Development and progression Gene therapy Genetic aspects Health aspects Immunoglobulins Medical research Metastasis Mortality Protein expression Proteins Tumors |
| title | Short interfering RNA-mediated silencing of actin-related protein 2/3 complex subunit 4 inhibits the migration of SW620 human colorectal cancer cells |
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