One-step generation of conditional and reversible gene knockouts

The combination of knocking one allele out with CRISPR-mediated NHEJ and targeting the other with a conditionally inactivating cassette allows rapid generation of conditional alleles. Loss-of-function studies are key for investigating gene function, and CRISPR technology has made genome editing wide...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature methods 2017-03, Vol.14 (3), p.287-289
Hauptverfasser:	Andersson-Rolf, Amanda, Mustata, Roxana C, Merenda, Alessandra, Kim, Jihoon, Perera, Sajith, Grego, Tiago, Andrews, Katie, Tremble, Katie, Silva, José C R, Fink, Juergen, Skarnes, William C, Koo, Bon-Kyoung
Format:	Artikel
Sprache:	eng
Schlagworte:	13/100 42/41 45/44 45/70 631/1647/1511 631/1647/1513/1967 96/106 96/31 Animals beta Catenin - genetics Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology brief-communication Cell Line Cell regulation Cellular biology Clustered Regularly Interspaced Short Palindromic Repeats - genetics CRISPR-Cas Systems - genetics Embryonic Stem Cells - cytology Gene Editing - methods Gene Knockout Techniques - methods Gene mutation Genes Genetic aspects Genetic engineering Genome - genetics Genomes HEK293 Cells Humans Identification and classification Inactivation Life Sciences Methods Mice Proteomics Stem cells Technology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!