Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia
Reseda pentagyna is the only endemic species among the seven species of the genera Reseda found in Saudi Arabia. Probably no information is available on regeneration by conventional method of regeneration through seeds or cuttings. Therefore, alternative method of tissue culture was attempted to reg...
Gespeichert in:
| Veröffentlicht in: | Saudi journal of biological sciences 2018-01, Vol.25 (1), p.111-116 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 116 |
|---|---|
| container_issue | 1 |
| container_start_page | 111 |
| container_title | Saudi journal of biological sciences |
| container_volume | 25 |
| creator | Al-Qurainy, Fahad Nadeem, Mohammad Khan, Salim Alansi, Saleh Tarroum, Mohamed Al-Ameri, Abdulhafed A. Gaafar, Abdel-Rhman Z. Alshameri, Aref |
| description | Reseda pentagyna is the only endemic species among the seven species of the genera Reseda found in Saudi Arabia. Probably no information is available on regeneration by conventional method of regeneration through seeds or cuttings. Therefore, alternative method of tissue culture was attempted to regenerate and multiply the plant. High shoot regeneration (14.44shoots/explant) was obtained after four weeks, when shoot cuttings cultured on MS containing BA at 1.0µM. Other cytokinins e.g., Kn, 2iP and TDZ found to be less effective in bud induction and shoot multiplication. Individual shoots were rooted on MS medium supplemented with various auxins at 0.5–5.0µM concentrations. The IBA (1.5µM) supplemented MS media induced maximum (83.3%) rooting. The plantlets were acclimatized and hardened under greenhouse conditions in plastic pots containing soil and farm yard manure with 95.0% success. The protocol developed would help to multiply the plant as well as conserve them in natural habitat. This can also be utilized to obtain active constituents for pharmaceutics and genetic manipulations. |
| doi_str_mv | 10.1016/j.sjbs.2017.07.003 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5775100</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1319562X17301821</els_id><sourcerecordid>1993015096</sourcerecordid><originalsourceid>FETCH-LOGICAL-c477t-aa8a7040bb1d34475f30dd648a34e1c817037ae7178dcc61b0fe0153a704a123</originalsourceid><addsrcrecordid>eNp9Ud2K1DAUDqK44-gLCEouvbBj0rRNCyIsiz8LC8LMXngXTpPTmrGT1KQzMk_i65o646A3QiAh5_s553yEPOdsxRmv3mxXcdvGVc64XLF0mHhAFnnORSY5qx6SBRe8ycoq_3JFnsS4ZayqRc0fk6u8EbIRVbUgP9cwWkPHAdxEA_boMMBkvXtNDzBY8_tNfUfnymQ1tW7CPtjpSNsjvd1s1nQH4RuGSMEZqr2LGA4X1hojGqAjugn6o4MEougM7pLSybMP_od1fdKlG9gbS68DtBaekkcdDBGfne8luf_w_v7mU3b3-ePtzfVdpgsppwygBskK1rbciKKQZSeYMVVRgyiQ65pLJiSg5LI2Wle8ZR0yXoqZBDwXS_LuJDvu2x0anfoMMKgx2DTVUXmw6t-Ks19V7w-qlLLkaeNL8uosEPz3PcZJ7WzUOKTZ0O-j4k0jkiFrqgTNT1AdfIwBu4sNZ2oOVG3VHKiaA1UsHSYS6eXfDV4ofxJMgBcnAKZ_7OCCkDUr83nCt-d62uLBYlBRW3QajQ2oJ2W8_Z__L7TDv1M</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1993015096</pqid></control><display><type>article</type><title>Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia</title><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Al-Qurainy, Fahad ; Nadeem, Mohammad ; Khan, Salim ; Alansi, Saleh ; Tarroum, Mohamed ; Al-Ameri, Abdulhafed A. ; Gaafar, Abdel-Rhman Z. ; Alshameri, Aref</creator><creatorcontrib>Al-Qurainy, Fahad ; Nadeem, Mohammad ; Khan, Salim ; Alansi, Saleh ; Tarroum, Mohamed ; Al-Ameri, Abdulhafed A. ; Gaafar, Abdel-Rhman Z. ; Alshameri, Aref</creatorcontrib><description>Reseda pentagyna is the only endemic species among the seven species of the genera Reseda found in Saudi Arabia. Probably no information is available on regeneration by conventional method of regeneration through seeds or cuttings. Therefore, alternative method of tissue culture was attempted to regenerate and multiply the plant. High shoot regeneration (14.44shoots/explant) was obtained after four weeks, when shoot cuttings cultured on MS containing BA at 1.0µM. Other cytokinins e.g., Kn, 2iP and TDZ found to be less effective in bud induction and shoot multiplication. Individual shoots were rooted on MS medium supplemented with various auxins at 0.5–5.0µM concentrations. The IBA (1.5µM) supplemented MS media induced maximum (83.3%) rooting. The plantlets were acclimatized and hardened under greenhouse conditions in plastic pots containing soil and farm yard manure with 95.0% success. The protocol developed would help to multiply the plant as well as conserve them in natural habitat. This can also be utilized to obtain active constituents for pharmaceutics and genetic manipulations.</description><identifier>ISSN: 1319-562X</identifier><identifier>EISSN: 2213-7106</identifier><identifier>DOI: 10.1016/j.sjbs.2017.07.003</identifier><identifier>PMID: 29379366</identifier><language>eng</language><publisher>Riyadh, Saudi Arabia: Elsevier B.V</publisher><subject>Conservation ; Endemic ; Micropropagation ; Reseda pentagyna ; Tissue culture</subject><ispartof>Saudi journal of biological sciences, 2018-01, Vol.25 (1), p.111-116</ispartof><rights>2017 King Saud University</rights><rights>2017 King Saud University 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-aa8a7040bb1d34475f30dd648a34e1c817037ae7178dcc61b0fe0153a704a123</citedby><cites>FETCH-LOGICAL-c477t-aa8a7040bb1d34475f30dd648a34e1c817037ae7178dcc61b0fe0153a704a123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5775100/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1319562X17301821$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3537,27901,27902,53766,53768,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29379366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Al-Qurainy, Fahad</creatorcontrib><creatorcontrib>Nadeem, Mohammad</creatorcontrib><creatorcontrib>Khan, Salim</creatorcontrib><creatorcontrib>Alansi, Saleh</creatorcontrib><creatorcontrib>Tarroum, Mohamed</creatorcontrib><creatorcontrib>Al-Ameri, Abdulhafed A.</creatorcontrib><creatorcontrib>Gaafar, Abdel-Rhman Z.</creatorcontrib><creatorcontrib>Alshameri, Aref</creatorcontrib><title>Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia</title><title>Saudi journal of biological sciences</title><addtitle>Saudi J Biol Sci</addtitle><description>Reseda pentagyna is the only endemic species among the seven species of the genera Reseda found in Saudi Arabia. Probably no information is available on regeneration by conventional method of regeneration through seeds or cuttings. Therefore, alternative method of tissue culture was attempted to regenerate and multiply the plant. High shoot regeneration (14.44shoots/explant) was obtained after four weeks, when shoot cuttings cultured on MS containing BA at 1.0µM. Other cytokinins e.g., Kn, 2iP and TDZ found to be less effective in bud induction and shoot multiplication. Individual shoots were rooted on MS medium supplemented with various auxins at 0.5–5.0µM concentrations. The IBA (1.5µM) supplemented MS media induced maximum (83.3%) rooting. The plantlets were acclimatized and hardened under greenhouse conditions in plastic pots containing soil and farm yard manure with 95.0% success. The protocol developed would help to multiply the plant as well as conserve them in natural habitat. This can also be utilized to obtain active constituents for pharmaceutics and genetic manipulations.</description><subject>Conservation</subject><subject>Endemic</subject><subject>Micropropagation</subject><subject>Reseda pentagyna</subject><subject>Tissue culture</subject><issn>1319-562X</issn><issn>2213-7106</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9Ud2K1DAUDqK44-gLCEouvbBj0rRNCyIsiz8LC8LMXngXTpPTmrGT1KQzMk_i65o646A3QiAh5_s553yEPOdsxRmv3mxXcdvGVc64XLF0mHhAFnnORSY5qx6SBRe8ycoq_3JFnsS4ZayqRc0fk6u8EbIRVbUgP9cwWkPHAdxEA_boMMBkvXtNDzBY8_tNfUfnymQ1tW7CPtjpSNsjvd1s1nQH4RuGSMEZqr2LGA4X1hojGqAjugn6o4MEougM7pLSybMP_od1fdKlG9gbS68DtBaekkcdDBGfne8luf_w_v7mU3b3-ePtzfVdpgsppwygBskK1rbciKKQZSeYMVVRgyiQ65pLJiSg5LI2Wle8ZR0yXoqZBDwXS_LuJDvu2x0anfoMMKgx2DTVUXmw6t-Ks19V7w-qlLLkaeNL8uosEPz3PcZJ7WzUOKTZ0O-j4k0jkiFrqgTNT1AdfIwBu4sNZ2oOVG3VHKiaA1UsHSYS6eXfDV4ofxJMgBcnAKZ_7OCCkDUr83nCt-d62uLBYlBRW3QajQ2oJ2W8_Z__L7TDv1M</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Al-Qurainy, Fahad</creator><creator>Nadeem, Mohammad</creator><creator>Khan, Salim</creator><creator>Alansi, Saleh</creator><creator>Tarroum, Mohamed</creator><creator>Al-Ameri, Abdulhafed A.</creator><creator>Gaafar, Abdel-Rhman Z.</creator><creator>Alshameri, Aref</creator><general>Elsevier B.V</general><general>Saudi Biological Society</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>ADJCN</scope><scope>AHFXO</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180101</creationdate><title>Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia</title><author>Al-Qurainy, Fahad ; Nadeem, Mohammad ; Khan, Salim ; Alansi, Saleh ; Tarroum, Mohamed ; Al-Ameri, Abdulhafed A. ; Gaafar, Abdel-Rhman Z. ; Alshameri, Aref</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-aa8a7040bb1d34475f30dd648a34e1c817037ae7178dcc61b0fe0153a704a123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Conservation</topic><topic>Endemic</topic><topic>Micropropagation</topic><topic>Reseda pentagyna</topic><topic>Tissue culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al-Qurainy, Fahad</creatorcontrib><creatorcontrib>Nadeem, Mohammad</creatorcontrib><creatorcontrib>Khan, Salim</creatorcontrib><creatorcontrib>Alansi, Saleh</creatorcontrib><creatorcontrib>Tarroum, Mohamed</creatorcontrib><creatorcontrib>Al-Ameri, Abdulhafed A.</creatorcontrib><creatorcontrib>Gaafar, Abdel-Rhman Z.</creatorcontrib><creatorcontrib>Alshameri, Aref</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Saudi journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Qurainy, Fahad</au><au>Nadeem, Mohammad</au><au>Khan, Salim</au><au>Alansi, Saleh</au><au>Tarroum, Mohamed</au><au>Al-Ameri, Abdulhafed A.</au><au>Gaafar, Abdel-Rhman Z.</au><au>Alshameri, Aref</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia</atitle><jtitle>Saudi journal of biological sciences</jtitle><addtitle>Saudi J Biol Sci</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>25</volume><issue>1</issue><spage>111</spage><epage>116</epage><pages>111-116</pages><issn>1319-562X</issn><eissn>2213-7106</eissn><abstract>Reseda pentagyna is the only endemic species among the seven species of the genera Reseda found in Saudi Arabia. Probably no information is available on regeneration by conventional method of regeneration through seeds or cuttings. Therefore, alternative method of tissue culture was attempted to regenerate and multiply the plant. High shoot regeneration (14.44shoots/explant) was obtained after four weeks, when shoot cuttings cultured on MS containing BA at 1.0µM. Other cytokinins e.g., Kn, 2iP and TDZ found to be less effective in bud induction and shoot multiplication. Individual shoots were rooted on MS medium supplemented with various auxins at 0.5–5.0µM concentrations. The IBA (1.5µM) supplemented MS media induced maximum (83.3%) rooting. The plantlets were acclimatized and hardened under greenhouse conditions in plastic pots containing soil and farm yard manure with 95.0% success. The protocol developed would help to multiply the plant as well as conserve them in natural habitat. This can also be utilized to obtain active constituents for pharmaceutics and genetic manipulations.</abstract><cop>Riyadh, Saudi Arabia</cop><pub>Elsevier B.V</pub><pmid>29379366</pmid><doi>10.1016/j.sjbs.2017.07.003</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1319-562X |
| ispartof | Saudi journal of biological sciences, 2018-01, Vol.25 (1), p.111-116 |
| issn | 1319-562X 2213-7106 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5775100 |
| source | Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Conservation Endemic Micropropagation Reseda pentagyna Tissue culture |
| title | Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T23%3A04%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20plant%20regeneration,%20validation%20of%20genetic%20integrity%20by%20ISSR%20markers%20and%20conservation%20of%20Reseda%20pentagyna%20an%20endemic%20plant%20growing%20in%20Saudi%20Arabia&rft.jtitle=Saudi%20journal%20of%20biological%20sciences&rft.au=Al-Qurainy,%20Fahad&rft.date=2018-01-01&rft.volume=25&rft.issue=1&rft.spage=111&rft.epage=116&rft.pages=111-116&rft.issn=1319-562X&rft.eissn=2213-7106&rft_id=info:doi/10.1016/j.sjbs.2017.07.003&rft_dat=%3Cproquest_pubme%3E1993015096%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1993015096&rft_id=info:pmid/29379366&rft_els_id=S1319562X17301821&rfr_iscdi=true |