Epigallocatechin gallate (EGCG) reduces the intensity of pancreatic amyloid fibrils in human islet amyloid polypeptide (hIAPP) transgenic mice

The formation of amyloid fibrils by human islet amyloid polypeptide protein (hIAPP) has been implicated in pancreas dysfunction and diabetes. However, efficient treatment options to reduce amyloid fibrils in vivo are still lacking. Therefore, we tested the effect of epigallocatechin gallate (EGCG) o...

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Veröffentlicht in:Scientific reports 2018-01, Vol.8 (1), p.1116-12, Article 1116
Hauptverfasser: Franko, Andras, Rodriguez Camargo, Diana C., Böddrich, Annett, Garg, Divita, Rodriguez Camargo, Andres, Rathkolb, Birgit, Janik, Dirk, Aichler, Michaela, Feuchtinger, Annette, Neff, Frauke, Fuchs, Helmut, Wanker, Erich E., Reif, Bernd, Häring, Hans-Ulrich, Peter, Andreas, Hrabě de Angelis, Martin
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container_issue 1
container_start_page 1116
container_title Scientific reports
container_volume 8
creator Franko, Andras
Rodriguez Camargo, Diana C.
Böddrich, Annett
Garg, Divita
Rodriguez Camargo, Andres
Rathkolb, Birgit
Janik, Dirk
Aichler, Michaela
Feuchtinger, Annette
Neff, Frauke
Fuchs, Helmut
Wanker, Erich E.
Reif, Bernd
Häring, Hans-Ulrich
Peter, Andreas
Hrabě de Angelis, Martin
description The formation of amyloid fibrils by human islet amyloid polypeptide protein (hIAPP) has been implicated in pancreas dysfunction and diabetes. However, efficient treatment options to reduce amyloid fibrils in vivo are still lacking. Therefore, we tested the effect of epigallocatechin gallate (EGCG) on fibril formation in vitro and in vivo . To determine the binding of hIAPP and EGCG, in vitro interaction studies were performed. To inhibit amyloid plaque formation in vivo , homozygous (tg/tg), hemizygous (wt/tg), and control mice (wt/wt) were treated with EGCG. EGCG bound to hIAPP in vitro and induced formation of amorphous aggregates instead of amyloid fibrils. Amyloid fibrils were detected in the pancreatic islets of tg/tg mice, which was associated with disrupted islet structure and diabetes. Although pancreatic amyloid fibrils could be detected in wt/tg mice, these animals were non-diabetic. EGCG application decreased amyloid fibril intensity in wt/tg mice, however it was ineffective in tg/tg animals. Our data indicate that EGCG inhibits amyloid fibril formation in vitro and reduces fibril intensity in non-diabetic wt/tg mice. These results demonstrate a possible in vivo effectiveness of EGCG on amyloid formation and suggest an early therapeutical application.
doi_str_mv 10.1038/s41598-017-18807-8
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subjects 119/118
13
13/1
13/51
14/3
140/131
631/45/56
631/57/2269
64/60
692/163/2743/137/1417
692/4017
Amylin
Amyloid - chemistry
Amyloid - metabolism
Amyloidosis - metabolism
Amyloidosis - pathology
Animals
Biomarkers
Catechin - analogs & derivatives
Catechin - chemistry
Catechin - metabolism
Catechin - pharmacology
Data processing
Diabetes
Diabetes mellitus
Epigallocatechin gallate
Fibrillogenesis
Humanities and Social Sciences
Humans
Islet Amyloid Polypeptide - genetics
Islet Amyloid Polypeptide - metabolism
Mice
Mice, Transgenic
Models, Molecular
Molecular Conformation
Molecular structure
multidisciplinary
Neuroprotective Agents - chemistry
Neuroprotective Agents - metabolism
Neuroprotective Agents - pharmacology
Pancreas
Pancreas - metabolism
Pancreas - pathology
Pancreas - ultrastructure
Polypeptides
Protein Binding
Rodents
Science
Science (multidisciplinary)
Transgenic mice
title Epigallocatechin gallate (EGCG) reduces the intensity of pancreatic amyloid fibrils in human islet amyloid polypeptide (hIAPP) transgenic mice
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