Mimicking Cartilage Tissue Zonal Organization by Engineering Tissue-Scale Gradient Hydrogels as 3D Cell Niche
Zonal organization plays an important role in cartilage structure and function, whereas most tissue-engineering strategies developed to date have only allowed the regeneration of cartilage with homogeneous biochemical and mechanical cues. To better restore tissue structure and function, there is a s...
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| Veröffentlicht in: | Tissue engineering. Part A 2018-01, Vol.24 (1-2), p.1-10 |
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| container_title | Tissue engineering. Part A |
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| creator | Zhu, Danqing Tong, Xinming Trinh, Pavin Yang, Fan |
| description | Zonal organization plays an important role in cartilage structure and function, whereas most tissue-engineering strategies developed to date have only allowed the regeneration of cartilage with homogeneous biochemical and mechanical cues. To better restore tissue structure and function, there is a strong need to engineer materials with biomimetic gradient niche cues that recapitulate native tissue organization. To address this critical unmet need, in this study, we report a method for rapid formation of tissue-scale gradient hydrogels as a three-dimensional (3D) cell niche with tunable biochemical and physical properties. When encapsulated in stiffness gradient hydrogels, both chondrocytes and mesenchymal stem cells demonstrated zone-specific response and extracellular deposition that mimics zonal organization of articular cartilage. Blocking cell mechanosensing using blebbistatin abolished the zonal response of chondrocytes in 3D hydrogels with a stiffness gradient. Such tissue-scale gradient hydrogels can provide a 3D artificial cell niche to enable tissue engineering of various tissue types with zonal organizations or tissue interfaces. |
| doi_str_mv | 10.1089/ten.tea.2016.0453 |
| format | Article |
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To better restore tissue structure and function, there is a strong need to engineer materials with biomimetic gradient niche cues that recapitulate native tissue organization. To address this critical unmet need, in this study, we report a method for rapid formation of tissue-scale gradient hydrogels as a three-dimensional (3D) cell niche with tunable biochemical and physical properties. When encapsulated in stiffness gradient hydrogels, both chondrocytes and mesenchymal stem cells demonstrated zone-specific response and extracellular deposition that mimics zonal organization of articular cartilage. Blocking cell mechanosensing using blebbistatin abolished the zonal response of chondrocytes in 3D hydrogels with a stiffness gradient. Such tissue-scale gradient hydrogels can provide a 3D artificial cell niche to enable tissue engineering of various tissue types with zonal organizations or tissue interfaces.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2016.0453</identifier><identifier>PMID: 28385124</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>3-D graphics ; Animals ; Arthritis ; Articular ; Bioengineering ; Biomedical materials ; Biomimetic materials ; Biomimetics ; Cartilage ; Cartilage (articular) ; Cattle ; Cells, Cultured ; Chondrocytes ; Chondrocytes - cytology ; Cues ; Extracellular matrix ; Hydrogels ; Hydrogels - chemistry ; Interfaces ; Kinases ; Mesenchyme ; Mimicry ; Original ; Original Articles ; Physical properties ; Polyethylene glycol ; Recovery of function ; Signal transduction ; Stem cells ; Structure-function relationships ; Surgery ; Tissue engineering ; Tissue Engineering - methods ; Tissues</subject><ispartof>Tissue engineering. 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Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Zonal organization plays an important role in cartilage structure and function, whereas most tissue-engineering strategies developed to date have only allowed the regeneration of cartilage with homogeneous biochemical and mechanical cues. To better restore tissue structure and function, there is a strong need to engineer materials with biomimetic gradient niche cues that recapitulate native tissue organization. To address this critical unmet need, in this study, we report a method for rapid formation of tissue-scale gradient hydrogels as a three-dimensional (3D) cell niche with tunable biochemical and physical properties. When encapsulated in stiffness gradient hydrogels, both chondrocytes and mesenchymal stem cells demonstrated zone-specific response and extracellular deposition that mimics zonal organization of articular cartilage. Blocking cell mechanosensing using blebbistatin abolished the zonal response of chondrocytes in 3D hydrogels with a stiffness gradient. Such tissue-scale gradient hydrogels can provide a 3D artificial cell niche to enable tissue engineering of various tissue types with zonal organizations or tissue interfaces.</description><subject>3-D graphics</subject><subject>Animals</subject><subject>Arthritis</subject><subject>Articular</subject><subject>Bioengineering</subject><subject>Biomedical materials</subject><subject>Biomimetic materials</subject><subject>Biomimetics</subject><subject>Cartilage</subject><subject>Cartilage (articular)</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Chondrocytes</subject><subject>Chondrocytes - cytology</subject><subject>Cues</subject><subject>Extracellular matrix</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Interfaces</subject><subject>Kinases</subject><subject>Mesenchyme</subject><subject>Mimicry</subject><subject>Original</subject><subject>Original Articles</subject><subject>Physical properties</subject><subject>Polyethylene glycol</subject><subject>Recovery of function</subject><subject>Signal transduction</subject><subject>Stem cells</subject><subject>Structure-function relationships</subject><subject>Surgery</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissues</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU9v1DAQxSMEoqXwAbggS1y4ZLHj-N8FCS2lRSr0QJEQF2viTFKXxGntBGn59DjasgJOHCxb4997mplXFM8Z3TCqzesZw2ZG2FSUyQ2tBX9QHDPDVcm5-Prw8K7ZUfEkpRtKJZVKPS6OKs21YFV9XIwf_ejddx96soU4-wF6JFc-pQXJtynAQC5jD8H_hNlPgTQ7chp6HxDjKtmD5WcHA5KzCK3HMJPzXRunHodEIBH-jmxxGMgn767xafGogyHhs_v7pPjy_vRqe15eXJ592L69KJ3QZi6VEFyjaDtsdcVb1klQkrVdo6tGSdGgoLzSXaNE3bayotIZp0EbWmnRIXT8pHiz971dmhFbl7uKMNjb6EeIOzuBt3__BH9t--mHFUpRakw2eHVvEKe7BdNsR59cngMCTkuyTGthasn1ir78B72Zlpg3lymjZS2NElWm2J5ycUopYndohlG7hmlzmPmAXcO0a5hZ8-LPKQ6K3-llQO2BtQwhDB4bjPN_WP8CQ6awlw</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Zhu, Danqing</creator><creator>Tong, Xinming</creator><creator>Trinh, Pavin</creator><creator>Yang, Fan</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180101</creationdate><title>Mimicking Cartilage Tissue Zonal Organization by Engineering Tissue-Scale Gradient Hydrogels as 3D Cell Niche</title><author>Zhu, Danqing ; Tong, Xinming ; Trinh, Pavin ; Yang, Fan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c589t-75538e5dfed823d1f6a761dfb82b765be50328fb754dd6206c9c8a890285feaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>3-D graphics</topic><topic>Animals</topic><topic>Arthritis</topic><topic>Articular</topic><topic>Bioengineering</topic><topic>Biomedical materials</topic><topic>Biomimetic materials</topic><topic>Biomimetics</topic><topic>Cartilage</topic><topic>Cartilage (articular)</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Chondrocytes</topic><topic>Chondrocytes - cytology</topic><topic>Cues</topic><topic>Extracellular matrix</topic><topic>Hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>Interfaces</topic><topic>Kinases</topic><topic>Mesenchyme</topic><topic>Mimicry</topic><topic>Original</topic><topic>Original Articles</topic><topic>Physical properties</topic><topic>Polyethylene glycol</topic><topic>Recovery of function</topic><topic>Signal transduction</topic><topic>Stem cells</topic><topic>Structure-function relationships</topic><topic>Surgery</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Danqing</creatorcontrib><creatorcontrib>Tong, Xinming</creatorcontrib><creatorcontrib>Trinh, Pavin</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Danqing</au><au>Tong, Xinming</au><au>Trinh, Pavin</au><au>Yang, Fan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mimicking Cartilage Tissue Zonal Organization by Engineering Tissue-Scale Gradient Hydrogels as 3D Cell Niche</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>24</volume><issue>1-2</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>Zonal organization plays an important role in cartilage structure and function, whereas most tissue-engineering strategies developed to date have only allowed the regeneration of cartilage with homogeneous biochemical and mechanical cues. To better restore tissue structure and function, there is a strong need to engineer materials with biomimetic gradient niche cues that recapitulate native tissue organization. To address this critical unmet need, in this study, we report a method for rapid formation of tissue-scale gradient hydrogels as a three-dimensional (3D) cell niche with tunable biochemical and physical properties. When encapsulated in stiffness gradient hydrogels, both chondrocytes and mesenchymal stem cells demonstrated zone-specific response and extracellular deposition that mimics zonal organization of articular cartilage. Blocking cell mechanosensing using blebbistatin abolished the zonal response of chondrocytes in 3D hydrogels with a stiffness gradient. 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| ispartof | Tissue engineering. Part A, 2018-01, Vol.24 (1-2), p.1-10 |
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| subjects | 3-D graphics Animals Arthritis Articular Bioengineering Biomedical materials Biomimetic materials Biomimetics Cartilage Cartilage (articular) Cattle Cells, Cultured Chondrocytes Chondrocytes - cytology Cues Extracellular matrix Hydrogels Hydrogels - chemistry Interfaces Kinases Mesenchyme Mimicry Original Original Articles Physical properties Polyethylene glycol Recovery of function Signal transduction Stem cells Structure-function relationships Surgery Tissue engineering Tissue Engineering - methods Tissues |
| title | Mimicking Cartilage Tissue Zonal Organization by Engineering Tissue-Scale Gradient Hydrogels as 3D Cell Niche |
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