Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
The dorsal root ganglia (DRG) consist of a multitude of sensory neuronal subtypes that function to relay sensory stimuli, including temperature, pressure, pain and position to the central nervous system. Our knowledge of DRG sensory neurons have been predominantly driven by animal studies and consid...
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| Veröffentlicht in: | Scientific reports 2018-01, Vol.8 (1), p.603-12, Article 603 |
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| creator | Alshawaf, Abdullah Jawad Viventi, Serena Qiu, Wanzhi D’Abaco, Giovanna Nayagam, Bryony Erlichster, Michael Chana, Gursharan Everall, Ian Ivanusic, Jason Skafidas, Efstratios Dottori, Mirella |
| description | The dorsal root ganglia (DRG) consist of a multitude of sensory neuronal subtypes that function to relay sensory stimuli, including temperature, pressure, pain and position to the central nervous system. Our knowledge of DRG sensory neurons have been predominantly driven by animal studies and considerably less is known about the human DRG. Human embryonic stem cells (hESC) are valuable resource to help close this gap. Our previous studies reported an efficient system for deriving neural crest and DRG sensory neurons from hESC. Here we show that this differentiation system gives rise to heterogeneous populations of sensory neuronal subtypes as demonstrated by phenotypic and functional analyses. Furthermore, using microelectrode arrays the maturation rate of the hESC-derived sensory neuronal cultures was monitored over 8 weeks in culture, showing their spontaneous firing activities starting at about 12 days post-differentiation and reaching maximum firing at about 6 weeks. These studies are highly valuable for developing an
in vitro
platform to study the diversity of sensory neuronal subtypes found within the human DRG. |
| doi_str_mv | 10.1038/s41598-017-19093-0 |
| format | Article |
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in vitro
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in vitro
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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alshawaf, Abdullah Jawad</au><au>Viventi, Serena</au><au>Qiu, Wanzhi</au><au>D’Abaco, Giovanna</au><au>Nayagam, Bryony</au><au>Erlichster, Michael</au><au>Chana, Gursharan</au><au>Everall, Ian</au><au>Ivanusic, Jason</au><au>Skafidas, Efstratios</au><au>Dottori, Mirella</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2018-01-12</date><risdate>2018</risdate><volume>8</volume><issue>1</issue><spage>603</spage><epage>12</epage><pages>603-12</pages><artnum>603</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The dorsal root ganglia (DRG) consist of a multitude of sensory neuronal subtypes that function to relay sensory stimuli, including temperature, pressure, pain and position to the central nervous system. Our knowledge of DRG sensory neurons have been predominantly driven by animal studies and considerably less is known about the human DRG. Human embryonic stem cells (hESC) are valuable resource to help close this gap. Our previous studies reported an efficient system for deriving neural crest and DRG sensory neurons from hESC. Here we show that this differentiation system gives rise to heterogeneous populations of sensory neuronal subtypes as demonstrated by phenotypic and functional analyses. Furthermore, using microelectrode arrays the maturation rate of the hESC-derived sensory neuronal cultures was monitored over 8 weeks in culture, showing their spontaneous firing activities starting at about 12 days post-differentiation and reaching maximum firing at about 6 weeks. These studies are highly valuable for developing an
in vitro
platform to study the diversity of sensory neuronal subtypes found within the human DRG.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29330377</pmid><doi>10.1038/s41598-017-19093-0</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-4263-9972</orcidid><orcidid>https://orcid.org/0000-0003-0598-4195</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/100 13/106 14/19 38 38/77 631/136/142 631/378/1959/2605 631/378/3917 631/532/1360 82/51 9/30 Biomarkers - metabolism Cell culture Cell Culture Techniques - methods Cell Differentiation Cell Line Central nervous system Dorsal root ganglia Embryo cells Gene expression Human Embryonic Stem Cells - cytology Human Embryonic Stem Cells - metabolism Humanities and Social Sciences Humans Microelectrodes multidisciplinary Neural crest Pain Peripheral Nervous System - cytology Phenotype Science Science (multidisciplinary) Sensory neurons Sensory Receptor Cells - cytology Sensory Receptor Cells - metabolism Sensory stimuli Stem cell transplantation Stem cells |
| title | Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells |
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