Immunohistochemical evaluation of inducible nitric oxide synthase in the epithelial lining of odontogenic cysts: A qualitative and quantitative analysis
The three common odontogenic cysts include radicular cysts (RCs), dentigerous cysts (DCs), and odontogenic keratocysts (OKCs). Among these 3 cysts, OKC is recently been classified as benign keratocystic odontogenic tumor attributing to its aggressive behavior, recurrence rate, and malignant potentia...
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| Veröffentlicht in: | Journal of oral and maxillofacial pathology : JOMFP 2017-09, Vol.21 (3), p.375-381 |
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| creator | Akshatha, B K Karuppiah, Karpagaselvi Manjunath, G S Kumarswamy, Jayalakshmi Papaiah, Lokesh Rao, Jyothi |
| description | The three common odontogenic cysts include radicular cysts (RCs), dentigerous cysts (DCs), and odontogenic keratocysts (OKCs). Among these 3 cysts, OKC is recently been classified as benign keratocystic odontogenic tumor attributing to its aggressive behavior, recurrence rate, and malignant potential. The present study involved qualitative and quantitative analysis of inducible nitric oxide synthase (iNOS) expression in epithelial lining of RCs, DCs, and OKCs, compare iNOS expression in epithelial linings of all the 3 cysts and determined overexpression of iNOS in OKCs which might contribute to its aggressive behavior and malignant potential.
The present study is to investigate the role of iNOS in the pathogenesis of OKCs, DCs, and RCs by evaluating the iNOS expression in the epithelial lining of these cysts.
Analysis of iNOS expression in epithelial lining cells of 20 RCs, 20 DCs, and 20 OKCs using immunohistochemistry done.
The percentage of positive cells and intensity of stain was assessed and compared among all the 3 cysts using contingency coefficient. Kappa statistics for the two observers were computed for finding interobserver agreement.
The percentage of iNOS-positive cells was found to be remarkably high in OKCs (12/20) -57.1% as compared to RCs (6/20) - 28.6% and DCs (3/20) - 14.3%. The interobserver agreement for iNOS-positive percentage cells was arrived with kappa values with OKCs → Statistically significant (
> 0.000), RCs → statistically significant (
> 0.001) with no significant values for DCs. No statistical difference exists among 3 study samples in regard to the intensity of staining with iNOS.
Increased iNOS expression in OKCs may contribute to bone resorption and accumulation of wild-type p53, hence, making OKCs more aggressive. |
| doi_str_mv | 10.4103/jomfp.JOMFP_96_17 |
| format | Article |
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The present study is to investigate the role of iNOS in the pathogenesis of OKCs, DCs, and RCs by evaluating the iNOS expression in the epithelial lining of these cysts.
Analysis of iNOS expression in epithelial lining cells of 20 RCs, 20 DCs, and 20 OKCs using immunohistochemistry done.
The percentage of positive cells and intensity of stain was assessed and compared among all the 3 cysts using contingency coefficient. Kappa statistics for the two observers were computed for finding interobserver agreement.
The percentage of iNOS-positive cells was found to be remarkably high in OKCs (12/20) -57.1% as compared to RCs (6/20) - 28.6% and DCs (3/20) - 14.3%. The interobserver agreement for iNOS-positive percentage cells was arrived with kappa values with OKCs → Statistically significant (
> 0.000), RCs → statistically significant (
> 0.001) with no significant values for DCs. No statistical difference exists among 3 study samples in regard to the intensity of staining with iNOS.
Increased iNOS expression in OKCs may contribute to bone resorption and accumulation of wild-type p53, hence, making OKCs more aggressive.</description><identifier>ISSN: 0973-029X</identifier><identifier>EISSN: 1998-393X</identifier><identifier>DOI: 10.4103/jomfp.JOMFP_96_17</identifier><identifier>PMID: 29391711</identifier><language>eng</language><publisher>India: Medknow Publications and Media Pvt. Ltd</publisher><subject>Analysis ; Angiogenesis ; Bone resorption ; Cold ; Cysts ; Cytokines ; DNA damage ; Enzymes ; Immunohistochemistry ; Mutation ; Nitric oxide ; Nitric-oxide synthase ; Odontogenic tumors ; Original ; p53 Protein ; Pathology ; Proteins ; Qualitative research ; Quantitative analysis ; Research centers ; Rodents ; Statistical analysis ; Statistics ; Studies ; Tumors</subject><ispartof>Journal of oral and maxillofacial pathology : JOMFP, 2017-09, Vol.21 (3), p.375-381</ispartof><rights>COPYRIGHT 2017 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt. Ltd. Sep/Dec 2017</rights><rights>Copyright: © 2017 Journal of Oral and Maxillofacial Pathology 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4097-9116bb09a9deecc33e581a7f8c90eae2d88983d6d8a6ce238ec70a16c138e3253</citedby><cites>FETCH-LOGICAL-c4097-9116bb09a9deecc33e581a7f8c90eae2d88983d6d8a6ce238ec70a16c138e3253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763859/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5763859/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29391711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akshatha, B K</creatorcontrib><creatorcontrib>Karuppiah, Karpagaselvi</creatorcontrib><creatorcontrib>Manjunath, G S</creatorcontrib><creatorcontrib>Kumarswamy, Jayalakshmi</creatorcontrib><creatorcontrib>Papaiah, Lokesh</creatorcontrib><creatorcontrib>Rao, Jyothi</creatorcontrib><title>Immunohistochemical evaluation of inducible nitric oxide synthase in the epithelial lining of odontogenic cysts: A qualitative and quantitative analysis</title><title>Journal of oral and maxillofacial pathology : JOMFP</title><addtitle>J Oral Maxillofac Pathol</addtitle><description>The three common odontogenic cysts include radicular cysts (RCs), dentigerous cysts (DCs), and odontogenic keratocysts (OKCs). Among these 3 cysts, OKC is recently been classified as benign keratocystic odontogenic tumor attributing to its aggressive behavior, recurrence rate, and malignant potential. The present study involved qualitative and quantitative analysis of inducible nitric oxide synthase (iNOS) expression in epithelial lining of RCs, DCs, and OKCs, compare iNOS expression in epithelial linings of all the 3 cysts and determined overexpression of iNOS in OKCs which might contribute to its aggressive behavior and malignant potential.
The present study is to investigate the role of iNOS in the pathogenesis of OKCs, DCs, and RCs by evaluating the iNOS expression in the epithelial lining of these cysts.
Analysis of iNOS expression in epithelial lining cells of 20 RCs, 20 DCs, and 20 OKCs using immunohistochemistry done.
The percentage of positive cells and intensity of stain was assessed and compared among all the 3 cysts using contingency coefficient. Kappa statistics for the two observers were computed for finding interobserver agreement.
The percentage of iNOS-positive cells was found to be remarkably high in OKCs (12/20) -57.1% as compared to RCs (6/20) - 28.6% and DCs (3/20) - 14.3%. The interobserver agreement for iNOS-positive percentage cells was arrived with kappa values with OKCs → Statistically significant (
> 0.000), RCs → statistically significant (
> 0.001) with no significant values for DCs. No statistical difference exists among 3 study samples in regard to the intensity of staining with iNOS.
Increased iNOS expression in OKCs may contribute to bone resorption and accumulation of wild-type p53, hence, making OKCs more aggressive.</description><subject>Analysis</subject><subject>Angiogenesis</subject><subject>Bone resorption</subject><subject>Cold</subject><subject>Cysts</subject><subject>Cytokines</subject><subject>DNA damage</subject><subject>Enzymes</subject><subject>Immunohistochemistry</subject><subject>Mutation</subject><subject>Nitric oxide</subject><subject>Nitric-oxide synthase</subject><subject>Odontogenic tumors</subject><subject>Original</subject><subject>p53 Protein</subject><subject>Pathology</subject><subject>Proteins</subject><subject>Qualitative research</subject><subject>Quantitative analysis</subject><subject>Research centers</subject><subject>Rodents</subject><subject>Statistical analysis</subject><subject>Statistics</subject><subject>Studies</subject><subject>Tumors</subject><issn>0973-029X</issn><issn>1998-393X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptUstuFDEQHCEQWQIfwAVZ4pLLLH7Mw-aAtIoIBAWFA0i5WV67Z8crj70Ze1bsn_C5eEjIAyEf2t1dVVa7qyheE7ysCGbvtmHodssvl1_PvknRSNI-KRZECF4ywa6eFgssWlZiKq6OihcxbjGueVXT58URFUyQlpBF8et8GCYfehtT0D0MViuHYK_cpJINHoUOWW8mbdcOkLdptBqFn9YAigefehUh91HqAcHO5uBs5jvrrd_M3GCCT2EDPtP0Iab4Hq3Q9aScTVl_D0h5M-c-3ReUO0QbXxbPOuUivLqNx8WPs4_fTz-XF5efzk9XF6Wu8nilIKRZr7FQwgBozRjUnKi241pgUEAN54Iz0xiuGg2UcdAtVqTRJF8Zrdlx8eFGdzetBzAafBqVk7vRDmo8yKCsfNzxtpebsJd12zBeiyxwciswhusJYpKDjRqcUx7CFGVeCBOCNjXN0Lf_QLdhGvPAM6oVFSOcsnvURjmQ1nchv6tnUbmqieBVS3mbUcv_oPIx8xKDh87m-iMCuSHoMcQ4Qnc3I8FytpP8Yyf5wE6Z8-bh59wx_vqH_QbN4cy7</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Akshatha, B K</creator><creator>Karuppiah, Karpagaselvi</creator><creator>Manjunath, G S</creator><creator>Kumarswamy, Jayalakshmi</creator><creator>Papaiah, Lokesh</creator><creator>Rao, Jyothi</creator><general>Medknow Publications and Media Pvt. 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Among these 3 cysts, OKC is recently been classified as benign keratocystic odontogenic tumor attributing to its aggressive behavior, recurrence rate, and malignant potential. The present study involved qualitative and quantitative analysis of inducible nitric oxide synthase (iNOS) expression in epithelial lining of RCs, DCs, and OKCs, compare iNOS expression in epithelial linings of all the 3 cysts and determined overexpression of iNOS in OKCs which might contribute to its aggressive behavior and malignant potential.
The present study is to investigate the role of iNOS in the pathogenesis of OKCs, DCs, and RCs by evaluating the iNOS expression in the epithelial lining of these cysts.
Analysis of iNOS expression in epithelial lining cells of 20 RCs, 20 DCs, and 20 OKCs using immunohistochemistry done.
The percentage of positive cells and intensity of stain was assessed and compared among all the 3 cysts using contingency coefficient. Kappa statistics for the two observers were computed for finding interobserver agreement.
The percentage of iNOS-positive cells was found to be remarkably high in OKCs (12/20) -57.1% as compared to RCs (6/20) - 28.6% and DCs (3/20) - 14.3%. The interobserver agreement for iNOS-positive percentage cells was arrived with kappa values with OKCs → Statistically significant (
> 0.000), RCs → statistically significant (
> 0.001) with no significant values for DCs. No statistical difference exists among 3 study samples in regard to the intensity of staining with iNOS.
Increased iNOS expression in OKCs may contribute to bone resorption and accumulation of wild-type p53, hence, making OKCs more aggressive.</abstract><cop>India</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>29391711</pmid><doi>10.4103/jomfp.JOMFP_96_17</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of oral and maxillofacial pathology : JOMFP, 2017-09, Vol.21 (3), p.375-381 |
| issn | 0973-029X 1998-393X |
| language | eng |
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| source | Medknow Open Access Medical Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Analysis Angiogenesis Bone resorption Cold Cysts Cytokines DNA damage Enzymes Immunohistochemistry Mutation Nitric oxide Nitric-oxide synthase Odontogenic tumors Original p53 Protein Pathology Proteins Qualitative research Quantitative analysis Research centers Rodents Statistical analysis Statistics Studies Tumors |
| title | Immunohistochemical evaluation of inducible nitric oxide synthase in the epithelial lining of odontogenic cysts: A qualitative and quantitative analysis |
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