Evaluation of fluorescence in situ hybridization techniques to study long non-coding RNA expression in cultured cells

Abstract Deciphering the functions of long non-coding RNAs (lncRNAs) is facilitated by visualization of their subcellular localization using in situ hybridization (ISH) techniques. We evaluated four different ISH methods for detection of MALAT1 and CYTOR in cultured cells: a multiple probe detection...

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Veröffentlicht in:	Nucleic acids research 2018-01, Vol.46 (1), p.e4-e4
Hauptverfasser:	Soares, Ricardo J, Maglieri, Giulia, Gutschner, Tony, Diederichs, Sven, Lund, Anders H, Nielsen, Boye S, Holmstrøm, Kim
Format:	Artikel
Sprache:	eng
Schlagworte:	A549 Cells Cell Line Cell Line, Tumor Cell Nucleus - genetics DNA Probes - genetics Gene Amplification Gene Expression Regulation HeLa Cells Humans In Situ Hybridization, Fluorescence - methods MCF-7 Cells Methods Online Reproducibility of Results RNA, Long Noncoding - genetics
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