Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181
Purpose FCGRT encodes the alpha-chain component of the neonatal Fc receptor (FcRn). FcRn is critical for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues. Few regulators of FcRn expression have been identified. We investigated the epigenetic regulation of FcRn...
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| Veröffentlicht in: | Pharmaceutical research 2018-01, Vol.35 (1), p.15-9, Article 15 |
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| creator | Ferguson, Daniel C. Blanco, Javier G. |
| description | Purpose
FCGRT
encodes the alpha-chain component of the neonatal Fc receptor (FcRn). FcRn is critical for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues. Few regulators of FcRn expression have been identified. We investigated the epigenetic regulation of FcRn by two microRNAs (
hsa-miR-3181
and
hsa-miR-3136-3p
) acting on
FCGRT
.
Methods
The binding of candidate microRNAs to the 3′-untranslated region of
FCGRT
was evaluated using luciferase reporter constructs in CHO cells. The effect of microRNAs on
FCGRT
mRNA and FcRn protein expression was evaluated using specific microRNA mimics and inhibitor transfections in A549, HEK293 and HepG2 cells.
Results
Hsa-miR-3181
mimic reduced luciferase reporter activity by 70.1% (10 nM,
P
|
| doi_str_mv | 10.1007/s11095-017-2294-0 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5757240</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A724211639</galeid><sourcerecordid>A724211639</sourcerecordid><originalsourceid>FETCH-LOGICAL-c537t-f30a060c372b80e45210c5c88a6f547c7fd7aaa75f55cfd0ff0644c4283496383</originalsourceid><addsrcrecordid>eNp1klGLEzEUhYMobl39Ab5IwBdfst5MksnkRSjFdoV1hVLBt5CmSZtlJqmTGWH_vRm6ll1R8hBIvntu7slB6C2FKwogP2ZKQQkCVJKqUpzAMzSjQjKigP94jmYgK04ayekFepXzHQA0VPGX6KJSDCop1Axt1m4_tmYIKeLk8XBw-HrsTMRLS25dimYwLV47645D6rFpjwdDFgcTIl656PBysVpv8PYefw22T-vbOWG0oa_RC2_a7N487Jfo-_LzZnFNbr6tvizmN8QKJgfiGRiowTJZbRtwXFQUrLBNY2ovuLTS76QxRgovhPU78B5qzi2vGsZVzRp2iT6ddI_jtnM76-LQm1Yf-9CZ_l4nE_TTmxgOep9-aSFFsQaKwIcHgT79HF0edBeydW1roktj1lQ1qjxVclHQ93-hd2nsYxlvongtlSqunqm9aZ0O0afS106iel46VpTWbKKu_kGVtXNdsCk6H8r5kwJ6Kigm59w7f56Rgp6ioE9R0CUKeoqCnmZ799icc8Wfvy9AdQJyuYp71z-a6L-qvwFRd7p-</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1984679929</pqid></control><display><type>article</type><title>Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181</title><source>MEDLINE</source><source>SpringerLink</source><creator>Ferguson, Daniel C. ; Blanco, Javier G.</creator><creatorcontrib>Ferguson, Daniel C. ; Blanco, Javier G.</creatorcontrib><description>Purpose
FCGRT
encodes the alpha-chain component of the neonatal Fc receptor (FcRn). FcRn is critical for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues. Few regulators of FcRn expression have been identified. We investigated the epigenetic regulation of FcRn by two microRNAs (
hsa-miR-3181
and
hsa-miR-3136-3p
) acting on
FCGRT
.
Methods
The binding of candidate microRNAs to the 3′-untranslated region of
FCGRT
was evaluated using luciferase reporter constructs in CHO cells. The effect of microRNAs on
FCGRT
mRNA and FcRn protein expression was evaluated using specific microRNA mimics and inhibitor transfections in A549, HEK293 and HepG2 cells.
Results
Hsa-miR-3181
mimic reduced luciferase reporter activity by 70.1% (10 nM,
P
< 0.0001). In A549, HEK293 and HepG2 cells,
hsa-miR-3181
decreased
FCGRT
mRNA expression (48.6%, 51.3% and 43.5% respectively, 25 nM,
P
< 0.05). The
hsa-miR-3181
mimic decreased the expression of FcRn protein by 40% after 48 h (25 nM,
P
< 0.001). The mature form of
hsa-miR-3181
was detected in samples of human liver.
Conclusions
These data suggest that
hsa-miR-3181
is an epigenetic regulator of
FCGRT
expression. The identification of this regulator of
FCGRT
may provide insights into a potential determinant of interindividual variability in FcRn expression.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1007/s11095-017-2294-0</identifier><identifier>PMID: 29302759</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>3' Untranslated Regions ; A549 Cells ; Albumin ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; CHO Cells ; Cricetinae ; Cricetulus ; Epigenesis, Genetic ; Epigenetic inheritance ; Epigenetics ; Fc receptors ; Gene Expression ; HEK293 Cells ; Hep G2 Cells ; Histocompatibility Antigens Class I - biosynthesis ; Histocompatibility Antigens Class I - genetics ; Humans ; Immunoglobulin G ; Infants (Newborn) ; Liver ; Liver - metabolism ; Luciferases ; Medical Law ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Monoclonal antibodies ; Neonates ; Newborn babies ; Pharmacology/Toxicology ; Pharmacy ; Receptors, Fc - biosynthesis ; Receptors, Fc - genetics ; Research Paper ; RNA, Messenger - genetics ; Tissues ; Transfection - methods</subject><ispartof>Pharmaceutical research, 2018-01, Vol.35 (1), p.15-9, Article 15</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Pharmaceutical Research is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-f30a060c372b80e45210c5c88a6f547c7fd7aaa75f55cfd0ff0644c4283496383</citedby><cites>FETCH-LOGICAL-c537t-f30a060c372b80e45210c5c88a6f547c7fd7aaa75f55cfd0ff0644c4283496383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11095-017-2294-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11095-017-2294-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29302759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferguson, Daniel C.</creatorcontrib><creatorcontrib>Blanco, Javier G.</creatorcontrib><title>Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><addtitle>Pharm Res</addtitle><description>Purpose
FCGRT
encodes the alpha-chain component of the neonatal Fc receptor (FcRn). FcRn is critical for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues. Few regulators of FcRn expression have been identified. We investigated the epigenetic regulation of FcRn by two microRNAs (
hsa-miR-3181
and
hsa-miR-3136-3p
) acting on
FCGRT
.
Methods
The binding of candidate microRNAs to the 3′-untranslated region of
FCGRT
was evaluated using luciferase reporter constructs in CHO cells. The effect of microRNAs on
FCGRT
mRNA and FcRn protein expression was evaluated using specific microRNA mimics and inhibitor transfections in A549, HEK293 and HepG2 cells.
Results
Hsa-miR-3181
mimic reduced luciferase reporter activity by 70.1% (10 nM,
P
< 0.0001). In A549, HEK293 and HepG2 cells,
hsa-miR-3181
decreased
FCGRT
mRNA expression (48.6%, 51.3% and 43.5% respectively, 25 nM,
P
< 0.05). The
hsa-miR-3181
mimic decreased the expression of FcRn protein by 40% after 48 h (25 nM,
P
< 0.001). The mature form of
hsa-miR-3181
was detected in samples of human liver.
Conclusions
These data suggest that
hsa-miR-3181
is an epigenetic regulator of
FCGRT
expression. The identification of this regulator of
FCGRT
may provide insights into a potential determinant of interindividual variability in FcRn expression.</description><subject>3' Untranslated Regions</subject><subject>A549 Cells</subject><subject>Albumin</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Epigenesis, Genetic</subject><subject>Epigenetic inheritance</subject><subject>Epigenetics</subject><subject>Fc receptors</subject><subject>Gene Expression</subject><subject>HEK293 Cells</subject><subject>Hep G2 Cells</subject><subject>Histocompatibility Antigens Class I - biosynthesis</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>Humans</subject><subject>Immunoglobulin G</subject><subject>Infants (Newborn)</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Luciferases</subject><subject>Medical Law</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Monoclonal antibodies</subject><subject>Neonates</subject><subject>Newborn babies</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Receptors, Fc - biosynthesis</subject><subject>Receptors, Fc - genetics</subject><subject>Research Paper</subject><subject>RNA, Messenger - genetics</subject><subject>Tissues</subject><subject>Transfection - methods</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1klGLEzEUhYMobl39Ab5IwBdfst5MksnkRSjFdoV1hVLBt5CmSZtlJqmTGWH_vRm6ll1R8hBIvntu7slB6C2FKwogP2ZKQQkCVJKqUpzAMzSjQjKigP94jmYgK04ayekFepXzHQA0VPGX6KJSDCop1Axt1m4_tmYIKeLk8XBw-HrsTMRLS25dimYwLV47645D6rFpjwdDFgcTIl656PBysVpv8PYefw22T-vbOWG0oa_RC2_a7N487Jfo-_LzZnFNbr6tvizmN8QKJgfiGRiowTJZbRtwXFQUrLBNY2ovuLTS76QxRgovhPU78B5qzi2vGsZVzRp2iT6ddI_jtnM76-LQm1Yf-9CZ_l4nE_TTmxgOep9-aSFFsQaKwIcHgT79HF0edBeydW1roktj1lQ1qjxVclHQ93-hd2nsYxlvongtlSqunqm9aZ0O0afS106iel46VpTWbKKu_kGVtXNdsCk6H8r5kwJ6Kigm59w7f56Rgp6ioE9R0CUKeoqCnmZ799icc8Wfvy9AdQJyuYp71z-a6L-qvwFRd7p-</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Ferguson, Daniel C.</creator><creator>Blanco, Javier G.</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20180101</creationdate><title>Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181</title><author>Ferguson, Daniel C. ; Blanco, Javier G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-f30a060c372b80e45210c5c88a6f547c7fd7aaa75f55cfd0ff0644c4283496383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>3' Untranslated Regions</topic><topic>A549 Cells</topic><topic>Albumin</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Epigenesis, Genetic</topic><topic>Epigenetic inheritance</topic><topic>Epigenetics</topic><topic>Fc receptors</topic><topic>Gene Expression</topic><topic>HEK293 Cells</topic><topic>Hep G2 Cells</topic><topic>Histocompatibility Antigens Class I - biosynthesis</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>Humans</topic><topic>Immunoglobulin G</topic><topic>Infants (Newborn)</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Luciferases</topic><topic>Medical Law</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Monoclonal antibodies</topic><topic>Neonates</topic><topic>Newborn babies</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Receptors, Fc - biosynthesis</topic><topic>Receptors, Fc - genetics</topic><topic>Research Paper</topic><topic>RNA, Messenger - genetics</topic><topic>Tissues</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferguson, Daniel C.</creatorcontrib><creatorcontrib>Blanco, Javier G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferguson, Daniel C.</au><au>Blanco, Javier G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181</atitle><jtitle>Pharmaceutical research</jtitle><stitle>Pharm Res</stitle><addtitle>Pharm Res</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>35</volume><issue>1</issue><spage>15</spage><epage>9</epage><pages>15-9</pages><artnum>15</artnum><issn>0724-8741</issn><eissn>1573-904X</eissn><abstract>Purpose
FCGRT
encodes the alpha-chain component of the neonatal Fc receptor (FcRn). FcRn is critical for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues. Few regulators of FcRn expression have been identified. We investigated the epigenetic regulation of FcRn by two microRNAs (
hsa-miR-3181
and
hsa-miR-3136-3p
) acting on
FCGRT
.
Methods
The binding of candidate microRNAs to the 3′-untranslated region of
FCGRT
was evaluated using luciferase reporter constructs in CHO cells. The effect of microRNAs on
FCGRT
mRNA and FcRn protein expression was evaluated using specific microRNA mimics and inhibitor transfections in A549, HEK293 and HepG2 cells.
Results
Hsa-miR-3181
mimic reduced luciferase reporter activity by 70.1% (10 nM,
P
< 0.0001). In A549, HEK293 and HepG2 cells,
hsa-miR-3181
decreased
FCGRT
mRNA expression (48.6%, 51.3% and 43.5% respectively, 25 nM,
P
< 0.05). The
hsa-miR-3181
mimic decreased the expression of FcRn protein by 40% after 48 h (25 nM,
P
< 0.001). The mature form of
hsa-miR-3181
was detected in samples of human liver.
Conclusions
These data suggest that
hsa-miR-3181
is an epigenetic regulator of
FCGRT
expression. The identification of this regulator of
FCGRT
may provide insights into a potential determinant of interindividual variability in FcRn expression.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>29302759</pmid><doi>10.1007/s11095-017-2294-0</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0724-8741 |
| ispartof | Pharmaceutical research, 2018-01, Vol.35 (1), p.15-9, Article 15 |
| issn | 0724-8741 1573-904X |
| language | eng |
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| source | MEDLINE; SpringerLink |
| subjects | 3' Untranslated Regions A549 Cells Albumin Animals Biochemistry Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine CHO Cells Cricetinae Cricetulus Epigenesis, Genetic Epigenetic inheritance Epigenetics Fc receptors Gene Expression HEK293 Cells Hep G2 Cells Histocompatibility Antigens Class I - biosynthesis Histocompatibility Antigens Class I - genetics Humans Immunoglobulin G Infants (Newborn) Liver Liver - metabolism Luciferases Medical Law MicroRNA MicroRNAs MicroRNAs - genetics miRNA Monoclonal antibodies Neonates Newborn babies Pharmacology/Toxicology Pharmacy Receptors, Fc - biosynthesis Receptors, Fc - genetics Research Paper RNA, Messenger - genetics Tissues Transfection - methods |
| title | Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181 |
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