Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA

The green alga Chlamydomonas reinhardtii is an invaluable reference organism to research fields including algal, plant, and ciliary biology. Accordingly, decades-long standing inefficiencies in targeted nuclear gene editing broadly hinder Chlamydomonas research. Here we report that single-step codel...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2017-12, Vol.114 (51), p.13567-13572
Hauptverfasser:	Ferenczi, Aron, Pyott, Douglas Euan, Xipnitou, Andromachi, Molnar, Attila
Format:	Artikel
Sprache:	eng
Schlagworte:	Algae Biological Sciences Chlamydomonas reinhardtii CRISPR Deoxyribonucleic acid DNA DNA repair Epitopes Genes Genetic modification Genetic transformation Mutation Reagents Ribonucleoproteins Single-stranded DNA Transgenes
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