UNBS5162 inhibits the proliferation of human A549 non‐small‐cell lung cancer cells by promoting apoptosis
Background Lung cancer is one of the most frequently diagnosed malignancies in the world, thus developing novel anticancer reagents for lung cancer treatment is critical. Methods We performed cell counting kit‐8 and cell colony formation assays to investigate the role of UNBS5162 in the proliferatio...
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| Veröffentlicht in: | Thoracic cancer 2018-01, Vol.9 (1), p.105-111 |
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| creator | Liu, Cuicui Xing, Jiaqiang Gao, Yujun |
| description | Background
Lung cancer is one of the most frequently diagnosed malignancies in the world, thus developing novel anticancer reagents for lung cancer treatment is critical.
Methods
We performed cell counting kit‐8 and cell colony formation assays to investigate the role of UNBS5162 in the proliferation of A549 cells. Invasion and migration assays were applied to study the inhibitory effect of UNBS5162 on non‐small cell lung cancer cells. To detect the effect of UNBS5162 on A549 cell apoptosis, Annexin‐V fluorescein isothiocyanate and propidium iodide staining methods were used. Protein expression was analyzed using Western blot assay.
Results
UNBS5162 not only inhibited proliferation but also decreased invasion and migration in A549 cells. Most cells were intact (96.93%) under control conditions, but the number of intact cells decreased (84.8%) after 24 hours of treatment with UNBS5162, and the number of early and late apoptotic cells significantly increased (P |
| doi_str_mv | 10.1111/1759-7714.12546 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5754305</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A737043489</galeid><sourcerecordid>A737043489</sourcerecordid><originalsourceid>FETCH-LOGICAL-g4206-62b4a2d8db4d4029b3092a14fea493f3cc00baaff85d71e8c25a482f6dc072403</originalsourceid><addsrcrecordid>eNptks2KFDEQx4Mo7rLu2ZsEvHiZMd_pXIRx8AsWPbh7Dul0MhNJJ22ne2VuPoLP6JOY3lkHF6wcqqj61Z8UVQA8x2iNq73GkquVlJitMeFMPALnp8zjU4zEGbgs5RuqRhuFCH8KzojCFAmGz0F_8_ntV44FgSHtQxumAqe9g8OYY_BuNFPICWYP93NvEtxwpmDK6ffPX6U3MVZvXYwwzmkHrUnWjXBJFNgeFo0-T6FWzJCHKZdQnoEn3sTiLu_9Bbh5_-56-3F19eXDp-3marVjBImVIC0zpGu6lnUMEdVSpIjBzDvDFPXUWoRaY7xveCexayzhhjXEi84iSRiiF-DNUXeY29511qVpNFEPY-jNeNDZBP2wksJe7_Kt5pIzingVeHUvMObvsyuT7kNZJjPJ5blorARlQiAqK_ryiO5MdDokn6uiXXC9kVQiRlmjKrX-D1Vf5_pgc3I-1PyDhhf_jnD6-9_VVYAfgR-183CqY6SX49DL-vVyCvruOPT1dnMX0D_aDax8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1963466037</pqid></control><display><type>article</type><title>UNBS5162 inhibits the proliferation of human A549 non‐small‐cell lung cancer cells by promoting apoptosis</title><source>Wiley Online Library - AutoHoldings Journals</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Liu, Cuicui ; Xing, Jiaqiang ; Gao, Yujun</creator><creatorcontrib>Liu, Cuicui ; Xing, Jiaqiang ; Gao, Yujun</creatorcontrib><description>Background
Lung cancer is one of the most frequently diagnosed malignancies in the world, thus developing novel anticancer reagents for lung cancer treatment is critical.
Methods
We performed cell counting kit‐8 and cell colony formation assays to investigate the role of UNBS5162 in the proliferation of A549 cells. Invasion and migration assays were applied to study the inhibitory effect of UNBS5162 on non‐small cell lung cancer cells. To detect the effect of UNBS5162 on A549 cell apoptosis, Annexin‐V fluorescein isothiocyanate and propidium iodide staining methods were used. Protein expression was analyzed using Western blot assay.
Results
UNBS5162 not only inhibited proliferation but also decreased invasion and migration in A549 cells. Most cells were intact (96.93%) under control conditions, but the number of intact cells decreased (84.8%) after 24 hours of treatment with UNBS5162, and the number of early and late apoptotic cells significantly increased (P < 0.05). Anti‐apoptotic protein Bcl‐2 expression in the UNBS5162 group was significantly decreased (P < 0.05), and expression of proapoptotic proteins Bim, Bax, and active caspase‐3 were significantly increased (P < 0.05) compared to the control. In the PI3K signaling pathway, phospo‐AKT and phospo‐mTOR levels were significantly decreased (P < 0.05), while S6K and Cyclin D1 protein levels were significantly decreased in UNBS5162 treated A549 cells (P < 0.05).
Conclusion
These findings suggest that UNBS5162 could inhibit A549 cell proliferation and metastasis by inhibiting PI3K pathway mediated apoptosis.</description><identifier>ISSN: 1759-7706</identifier><identifier>EISSN: 1759-7714</identifier><identifier>DOI: 10.1111/1759-7714.12546</identifier><identifier>PMID: 29130641</identifier><language>eng</language><publisher>Melbourne: John Wiley & Sons Australia, Ltd</publisher><subject>Analysis ; Apoptosis ; Cancer ; Cancer cells ; Care and treatment ; Chemical tests and reagents ; Fluorescein ; Lung cancer, Non-small cell ; Lung cancer, Small cell ; non‐small‐cell lung cancer ; Original ; PI3K pathway ; proliferation ; Proteins ; Respiratory agents ; UNBS5162</subject><ispartof>Thoracic cancer, 2018-01, Vol.9 (1), p.105-111</ispartof><rights>2017 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd</rights><rights>2017 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.</rights><rights>COPYRIGHT 2018 John Wiley & Sons, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5754305/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5754305/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11542,27903,27904,45553,45554,46031,46455,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29130641$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Cuicui</creatorcontrib><creatorcontrib>Xing, Jiaqiang</creatorcontrib><creatorcontrib>Gao, Yujun</creatorcontrib><title>UNBS5162 inhibits the proliferation of human A549 non‐small‐cell lung cancer cells by promoting apoptosis</title><title>Thoracic cancer</title><addtitle>Thorac Cancer</addtitle><description>Background
Lung cancer is one of the most frequently diagnosed malignancies in the world, thus developing novel anticancer reagents for lung cancer treatment is critical.
Methods
We performed cell counting kit‐8 and cell colony formation assays to investigate the role of UNBS5162 in the proliferation of A549 cells. Invasion and migration assays were applied to study the inhibitory effect of UNBS5162 on non‐small cell lung cancer cells. To detect the effect of UNBS5162 on A549 cell apoptosis, Annexin‐V fluorescein isothiocyanate and propidium iodide staining methods were used. Protein expression was analyzed using Western blot assay.
Results
UNBS5162 not only inhibited proliferation but also decreased invasion and migration in A549 cells. Most cells were intact (96.93%) under control conditions, but the number of intact cells decreased (84.8%) after 24 hours of treatment with UNBS5162, and the number of early and late apoptotic cells significantly increased (P < 0.05). Anti‐apoptotic protein Bcl‐2 expression in the UNBS5162 group was significantly decreased (P < 0.05), and expression of proapoptotic proteins Bim, Bax, and active caspase‐3 were significantly increased (P < 0.05) compared to the control. In the PI3K signaling pathway, phospo‐AKT and phospo‐mTOR levels were significantly decreased (P < 0.05), while S6K and Cyclin D1 protein levels were significantly decreased in UNBS5162 treated A549 cells (P < 0.05).
Conclusion
These findings suggest that UNBS5162 could inhibit A549 cell proliferation and metastasis by inhibiting PI3K pathway mediated apoptosis.</description><subject>Analysis</subject><subject>Apoptosis</subject><subject>Cancer</subject><subject>Cancer cells</subject><subject>Care and treatment</subject><subject>Chemical tests and reagents</subject><subject>Fluorescein</subject><subject>Lung cancer, Non-small cell</subject><subject>Lung cancer, Small cell</subject><subject>non‐small‐cell lung cancer</subject><subject>Original</subject><subject>PI3K pathway</subject><subject>proliferation</subject><subject>Proteins</subject><subject>Respiratory agents</subject><subject>UNBS5162</subject><issn>1759-7706</issn><issn>1759-7714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNptks2KFDEQx4Mo7rLu2ZsEvHiZMd_pXIRx8AsWPbh7Dul0MhNJJ22ne2VuPoLP6JOY3lkHF6wcqqj61Z8UVQA8x2iNq73GkquVlJitMeFMPALnp8zjU4zEGbgs5RuqRhuFCH8KzojCFAmGz0F_8_ntV44FgSHtQxumAqe9g8OYY_BuNFPICWYP93NvEtxwpmDK6ffPX6U3MVZvXYwwzmkHrUnWjXBJFNgeFo0-T6FWzJCHKZdQnoEn3sTiLu_9Bbh5_-56-3F19eXDp-3marVjBImVIC0zpGu6lnUMEdVSpIjBzDvDFPXUWoRaY7xveCexayzhhjXEi84iSRiiF-DNUXeY29511qVpNFEPY-jNeNDZBP2wksJe7_Kt5pIzingVeHUvMObvsyuT7kNZJjPJ5blorARlQiAqK_ryiO5MdDokn6uiXXC9kVQiRlmjKrX-D1Vf5_pgc3I-1PyDhhf_jnD6-9_VVYAfgR-183CqY6SX49DL-vVyCvruOPT1dnMX0D_aDax8</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Liu, Cuicui</creator><creator>Xing, Jiaqiang</creator><creator>Gao, Yujun</creator><general>John Wiley & Sons Australia, Ltd</general><general>John Wiley & Sons, Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201801</creationdate><title>UNBS5162 inhibits the proliferation of human A549 non‐small‐cell lung cancer cells by promoting apoptosis</title><author>Liu, Cuicui ; Xing, Jiaqiang ; Gao, Yujun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g4206-62b4a2d8db4d4029b3092a14fea493f3cc00baaff85d71e8c25a482f6dc072403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Analysis</topic><topic>Apoptosis</topic><topic>Cancer</topic><topic>Cancer cells</topic><topic>Care and treatment</topic><topic>Chemical tests and reagents</topic><topic>Fluorescein</topic><topic>Lung cancer, Non-small cell</topic><topic>Lung cancer, Small cell</topic><topic>non‐small‐cell lung cancer</topic><topic>Original</topic><topic>PI3K pathway</topic><topic>proliferation</topic><topic>Proteins</topic><topic>Respiratory agents</topic><topic>UNBS5162</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Cuicui</creatorcontrib><creatorcontrib>Xing, Jiaqiang</creatorcontrib><creatorcontrib>Gao, Yujun</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Thoracic cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Cuicui</au><au>Xing, Jiaqiang</au><au>Gao, Yujun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UNBS5162 inhibits the proliferation of human A549 non‐small‐cell lung cancer cells by promoting apoptosis</atitle><jtitle>Thoracic cancer</jtitle><addtitle>Thorac Cancer</addtitle><date>2018-01</date><risdate>2018</risdate><volume>9</volume><issue>1</issue><spage>105</spage><epage>111</epage><pages>105-111</pages><issn>1759-7706</issn><eissn>1759-7714</eissn><abstract>Background
Lung cancer is one of the most frequently diagnosed malignancies in the world, thus developing novel anticancer reagents for lung cancer treatment is critical.
Methods
We performed cell counting kit‐8 and cell colony formation assays to investigate the role of UNBS5162 in the proliferation of A549 cells. Invasion and migration assays were applied to study the inhibitory effect of UNBS5162 on non‐small cell lung cancer cells. To detect the effect of UNBS5162 on A549 cell apoptosis, Annexin‐V fluorescein isothiocyanate and propidium iodide staining methods were used. Protein expression was analyzed using Western blot assay.
Results
UNBS5162 not only inhibited proliferation but also decreased invasion and migration in A549 cells. Most cells were intact (96.93%) under control conditions, but the number of intact cells decreased (84.8%) after 24 hours of treatment with UNBS5162, and the number of early and late apoptotic cells significantly increased (P < 0.05). Anti‐apoptotic protein Bcl‐2 expression in the UNBS5162 group was significantly decreased (P < 0.05), and expression of proapoptotic proteins Bim, Bax, and active caspase‐3 were significantly increased (P < 0.05) compared to the control. In the PI3K signaling pathway, phospo‐AKT and phospo‐mTOR levels were significantly decreased (P < 0.05), while S6K and Cyclin D1 protein levels were significantly decreased in UNBS5162 treated A549 cells (P < 0.05).
Conclusion
These findings suggest that UNBS5162 could inhibit A549 cell proliferation and metastasis by inhibiting PI3K pathway mediated apoptosis.</abstract><cop>Melbourne</cop><pub>John Wiley & Sons Australia, Ltd</pub><pmid>29130641</pmid><doi>10.1111/1759-7714.12546</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; DOAJ Directory of Open Access Journals; Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Analysis Apoptosis Cancer Cancer cells Care and treatment Chemical tests and reagents Fluorescein Lung cancer, Non-small cell Lung cancer, Small cell non‐small‐cell lung cancer Original PI3K pathway proliferation Proteins Respiratory agents UNBS5162 |
| title | UNBS5162 inhibits the proliferation of human A549 non‐small‐cell lung cancer cells by promoting apoptosis |
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