Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis
There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whe...
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| Veröffentlicht in: | The Journal of cell biology 2018-01, Vol.217 (1), p.179-193 |
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| creator | Strauss, Bernhard Harrison, Andrew Coelho, Paula Almeida Yata, Keiko Zernicka-Goetz, Magdalena Pines, Jonathon |
| description | There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whether it is required for cell division is contentious. Here, we used a novel imaging approach to analyze Cyclin B1-null embryos from fertilization onward. We show that Cyclin B1
embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis. |
| doi_str_mv | 10.1083/jcb.201612147 |
| format | Article |
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embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis.</description><identifier>ISSN: 0021-9525</identifier><identifier>EISSN: 1540-8140</identifier><identifier>DOI: 10.1083/jcb.201612147</identifier><identifier>PMID: 29074707</identifier><language>eng</language><publisher>United States: Rockefeller University Press</publisher><subject>Active Transport, Cell Nucleus - physiology ; Animals ; CDC2 Protein Kinase - genetics ; cdc25 Phosphatases - metabolism ; Cell cycle ; Cell Cycle Proteins - metabolism ; Cell division ; Cyclin B1 ; Cyclin B1 - genetics ; Cyclins ; DNA-Binding Proteins - metabolism ; Embryonic Development - genetics ; Embryos ; Fertilization ; G2 phase ; Implantation ; Machinery and equipment ; Mice ; Mice, Knockout ; Mitosis ; Mitosis - genetics ; Mutation ; Nuclear Proteins - metabolism ; Nuclear transport ; Nuclei (cytology) ; Phosphorylation ; Protein-Tyrosine Kinases - metabolism ; Proteins ; Redundancy ; Regulators ; Transcription Factors - metabolism ; Transplants & implants</subject><ispartof>The Journal of cell biology, 2018-01, Vol.217 (1), p.179-193</ispartof><rights>2018 Strauss et al.</rights><rights>Copyright Rockefeller University Press Jan 2018</rights><rights>2018 Strauss et al. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-94cb87071cf4e72923c3acffc0155e69fd5319b221f63cc60737d5e5a8391c703</citedby><cites>FETCH-LOGICAL-c481t-94cb87071cf4e72923c3acffc0155e69fd5319b221f63cc60737d5e5a8391c703</cites><orcidid>0000-0002-5227-6004 ; 0000-0001-7648-4898 ; 0000-0003-0614-7575 ; 0000-0002-3778-8415</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29074707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Strauss, Bernhard</creatorcontrib><creatorcontrib>Harrison, Andrew</creatorcontrib><creatorcontrib>Coelho, Paula Almeida</creatorcontrib><creatorcontrib>Yata, Keiko</creatorcontrib><creatorcontrib>Zernicka-Goetz, Magdalena</creatorcontrib><creatorcontrib>Pines, Jonathon</creatorcontrib><title>Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis</title><title>The Journal of cell biology</title><addtitle>J Cell Biol</addtitle><description>There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. Cyclin B1 is essential for development, but whether it is required for cell division is contentious. Here, we used a novel imaging approach to analyze Cyclin B1-null embryos from fertilization onward. We show that Cyclin B1
embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis.</description><subject>Active Transport, Cell Nucleus - physiology</subject><subject>Animals</subject><subject>CDC2 Protein Kinase - genetics</subject><subject>cdc25 Phosphatases - metabolism</subject><subject>Cell cycle</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell division</subject><subject>Cyclin B1</subject><subject>Cyclin B1 - genetics</subject><subject>Cyclins</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Embryonic Development - genetics</subject><subject>Embryos</subject><subject>Fertilization</subject><subject>G2 phase</subject><subject>Implantation</subject><subject>Machinery and equipment</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Mitosis</subject><subject>Mitosis - genetics</subject><subject>Mutation</subject><subject>Nuclear Proteins - metabolism</subject><subject>Nuclear transport</subject><subject>Nuclei (cytology)</subject><subject>Phosphorylation</subject><subject>Protein-Tyrosine Kinases - metabolism</subject><subject>Proteins</subject><subject>Redundancy</subject><subject>Regulators</subject><subject>Transcription Factors - metabolism</subject><subject>Transplants & implants</subject><issn>0021-9525</issn><issn>1540-8140</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc2LFDEQxYMo7uzq0asEvHiw16p8dDoXQQe_YMGLnkM6U-1m6O6MSffi_PdGdx1WTwWvfjzq1WPsGcIlQidf70N_KQBbFKjMA7ZBraDpUMFDtgEQ2Fgt9Bk7L2UPAMoo-ZidCQtGGTAbRttjGOPM3yGPhVMpNC_Rj3xImU9xSaWqdT2ltRCnqc_HVF5xP-94XAqf1zCSz5x-HlJeeKGqLdfElzjRfYsn7NHgx0JP7-YF-_bh_dftp-bqy8fP27dXTVAdLo1Voe_qWRgGRUZYIYP0YRgCoNbU2mGnJdpeCBxaGUILRpqdJu07aTEYkBfsza3vYe0n2oUaJvvRHXKcfD665KP7dzPHa_c93ThtVGf_GLy8M8jpx0plcVMsgcbRz1Rf4NBW0kgUqqIv_kP3ac1zjVcpCx1qY9tKNbdUyKmUTMPpGAT3u0BXC3SnAiv__H6CE_23MfkLb0WW2w</recordid><startdate>20180102</startdate><enddate>20180102</enddate><creator>Strauss, Bernhard</creator><creator>Harrison, Andrew</creator><creator>Coelho, Paula Almeida</creator><creator>Yata, Keiko</creator><creator>Zernicka-Goetz, Magdalena</creator><creator>Pines, Jonathon</creator><general>Rockefeller University Press</general><general>The Rockefeller University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5227-6004</orcidid><orcidid>https://orcid.org/0000-0001-7648-4898</orcidid><orcidid>https://orcid.org/0000-0003-0614-7575</orcidid><orcidid>https://orcid.org/0000-0002-3778-8415</orcidid></search><sort><creationdate>20180102</creationdate><title>Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis</title><author>Strauss, Bernhard ; 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embryos arrest in G2 phase after just two divisions. This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis.</abstract><cop>United States</cop><pub>Rockefeller University Press</pub><pmid>29074707</pmid><doi>10.1083/jcb.201612147</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-5227-6004</orcidid><orcidid>https://orcid.org/0000-0001-7648-4898</orcidid><orcidid>https://orcid.org/0000-0003-0614-7575</orcidid><orcidid>https://orcid.org/0000-0002-3778-8415</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Active Transport, Cell Nucleus - physiology Animals CDC2 Protein Kinase - genetics cdc25 Phosphatases - metabolism Cell cycle Cell Cycle Proteins - metabolism Cell division Cyclin B1 Cyclin B1 - genetics Cyclins DNA-Binding Proteins - metabolism Embryonic Development - genetics Embryos Fertilization G2 phase Implantation Machinery and equipment Mice Mice, Knockout Mitosis Mitosis - genetics Mutation Nuclear Proteins - metabolism Nuclear transport Nuclei (cytology) Phosphorylation Protein-Tyrosine Kinases - metabolism Proteins Redundancy Regulators Transcription Factors - metabolism Transplants & implants |
| title | Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis |
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