Isolation and Characterization of Ftsz Genes in Cassava
The filamenting temperature-sensitive Z proteins (FtsZs) play an important role in plastid division. In this study, three FtsZ genes were isolated from the cassava genome, and named MeFtsZ1, MeFtsZ2-1, and MeFtsZ2-2, respectively. Based on phylogeny, the MeFtsZs were classified into two groups (FtsZ...
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| Veröffentlicht in: | Genes 2017-12, Vol.8 (12), p.391 |
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| creator | Geng, Meng-Ting Min, Yi Yao, Yuan Chen, Xia Fan, Jie Yuan, Shuai Wang, Lei Sun, Chong Zhang, Fan Shang, Lu Wang, Yun-Lin Li, Rui-Mei Fu, Shao-Ping Duan, Rui-Jun Liu, Jiao Hu, Xin-Wen Guo, Jian-Chun |
| description | The filamenting temperature-sensitive Z proteins (FtsZs) play an important role in plastid division. In this study, three FtsZ genes were isolated from the cassava genome, and named MeFtsZ1, MeFtsZ2-1, and MeFtsZ2-2, respectively. Based on phylogeny, the MeFtsZs were classified into two groups (FtsZ1 and FtsZ2). MeFtsZ1 with a putative signal peptide at N-terminal, has six exons, and is classed to FtsZ1 clade. MeFtsZ2-1 and MeFtsZ2-2 without a putative signal peptide, have seven exons, and are classed to FtsZ2 clade. Subcellular localization found that all the three MeFtsZs could locate in chloroplasts and form a ring in chloroplastids. Structure analysis found that all MeFtsZ proteins contain a conserved guanosine triphosphatase (GTPase) domain in favor of generate contractile force for cassava plastid division. The expression profiles of MeFtsZ genes by quantitative reverse transcription-PCR (qRT-PCR) analysis in photosynthetic and non-photosynthetic tissues found that all of the MeFtsZ genes had higher expression levels in photosynthetic tissues, especially in younger leaves, and lower expression levels in the non-photosynthetic tissues. During cassava storage root development, the expressions of MeFtsZ2-1 and MeFtsZ2-2 were comparatively higher than MeFtsZ1. The transformed Arabidopsis of MeFtsZ2-1 and MeFtsZ2-2 contained abnormally shape, fewer number, and larger volume chloroplasts. Phytohormones were involved in regulating the expressions of MeFtsZ genes. Therefore, we deduced that all of the
play an important role in chloroplast division, and that
2 (2-1, 2-2) might be involved in amyloplast division and regulated by phytohormones during cassava storage root development. |
| doi_str_mv | 10.3390/genes8120391 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5748709</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1977780776</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-8ce75f7024b7e7dbf0bb0a032bd413b7e5d4e9f834ac3531845d84d09ff0157a3</originalsourceid><addsrcrecordid>eNpdkU1LAzEQhoMottTePMuCFw-uTr5MchFksbVQ8KLnkN3Ntlu2m5psC_bXm9JaqnOZYebhnRlehK4xPFCq4HFmWxskJkAVPkN9AoKmjBF-flL30DCEBcRgQAD4JeoRRRgTFPpITIJrTFe7NjFtmWRz403RWV9v901XJaMubJPxblFSt0lmQjAbc4UuKtMEOzzkAfocvX5kb-n0fTzJXqZpwTDpUllYwSsBhOXCijKvIM_BACV5yTCNPV4yqypJmSkop1gyXkpWgqoqwFwYOkDPe93VOl_asrBt502jV75eGv-tnan130lbz_XMbTQXTApQUeDuIODd19qGTi_rUNimMa1166CxEkJIEOIporf_0IVb-za-FykpuWIKs0jd76nCuxC8rY7HYNA7U_SpKRG_OX3gCP9aQH8AsgmHdw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1988594914</pqid></control><display><type>article</type><title>Isolation and Characterization of Ftsz Genes in Cassava</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><creator>Geng, Meng-Ting ; Min, Yi ; Yao, Yuan ; Chen, Xia ; Fan, Jie ; Yuan, Shuai ; Wang, Lei ; Sun, Chong ; Zhang, Fan ; Shang, Lu ; Wang, Yun-Lin ; Li, Rui-Mei ; Fu, Shao-Ping ; Duan, Rui-Jun ; Liu, Jiao ; Hu, Xin-Wen ; Guo, Jian-Chun</creator><creatorcontrib>Geng, Meng-Ting ; Min, Yi ; Yao, Yuan ; Chen, Xia ; Fan, Jie ; Yuan, Shuai ; Wang, Lei ; Sun, Chong ; Zhang, Fan ; Shang, Lu ; Wang, Yun-Lin ; Li, Rui-Mei ; Fu, Shao-Ping ; Duan, Rui-Jun ; Liu, Jiao ; Hu, Xin-Wen ; Guo, Jian-Chun</creatorcontrib><description>The filamenting temperature-sensitive Z proteins (FtsZs) play an important role in plastid division. In this study, three FtsZ genes were isolated from the cassava genome, and named MeFtsZ1, MeFtsZ2-1, and MeFtsZ2-2, respectively. Based on phylogeny, the MeFtsZs were classified into two groups (FtsZ1 and FtsZ2). MeFtsZ1 with a putative signal peptide at N-terminal, has six exons, and is classed to FtsZ1 clade. MeFtsZ2-1 and MeFtsZ2-2 without a putative signal peptide, have seven exons, and are classed to FtsZ2 clade. Subcellular localization found that all the three MeFtsZs could locate in chloroplasts and form a ring in chloroplastids. Structure analysis found that all MeFtsZ proteins contain a conserved guanosine triphosphatase (GTPase) domain in favor of generate contractile force for cassava plastid division. The expression profiles of MeFtsZ genes by quantitative reverse transcription-PCR (qRT-PCR) analysis in photosynthetic and non-photosynthetic tissues found that all of the MeFtsZ genes had higher expression levels in photosynthetic tissues, especially in younger leaves, and lower expression levels in the non-photosynthetic tissues. During cassava storage root development, the expressions of MeFtsZ2-1 and MeFtsZ2-2 were comparatively higher than MeFtsZ1. The transformed Arabidopsis of MeFtsZ2-1 and MeFtsZ2-2 contained abnormally shape, fewer number, and larger volume chloroplasts. Phytohormones were involved in regulating the expressions of MeFtsZ genes. Therefore, we deduced that all of the
play an important role in chloroplast division, and that
2 (2-1, 2-2) might be involved in amyloplast division and regulated by phytohormones during cassava storage root development.</description><identifier>ISSN: 2073-4425</identifier><identifier>EISSN: 2073-4425</identifier><identifier>DOI: 10.3390/genes8120391</identifier><identifier>PMID: 29244730</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Cassava ; Chloroplasts ; Contractility ; Exons ; Genomes ; Guanosine ; Guanosine triphosphatases ; Localization ; Phylogeny ; Reverse transcription ; Triphosphatase</subject><ispartof>Genes, 2017-12, Vol.8 (12), p.391</ispartof><rights>Copyright MDPI AG 2017</rights><rights>2017 by the authors. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8ce75f7024b7e7dbf0bb0a032bd413b7e5d4e9f834ac3531845d84d09ff0157a3</citedby><cites>FETCH-LOGICAL-c412t-8ce75f7024b7e7dbf0bb0a032bd413b7e5d4e9f834ac3531845d84d09ff0157a3</cites><orcidid>0000-0003-1791-0433</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748709/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748709/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29244730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geng, Meng-Ting</creatorcontrib><creatorcontrib>Min, Yi</creatorcontrib><creatorcontrib>Yao, Yuan</creatorcontrib><creatorcontrib>Chen, Xia</creatorcontrib><creatorcontrib>Fan, Jie</creatorcontrib><creatorcontrib>Yuan, Shuai</creatorcontrib><creatorcontrib>Wang, Lei</creatorcontrib><creatorcontrib>Sun, Chong</creatorcontrib><creatorcontrib>Zhang, Fan</creatorcontrib><creatorcontrib>Shang, Lu</creatorcontrib><creatorcontrib>Wang, Yun-Lin</creatorcontrib><creatorcontrib>Li, Rui-Mei</creatorcontrib><creatorcontrib>Fu, Shao-Ping</creatorcontrib><creatorcontrib>Duan, Rui-Jun</creatorcontrib><creatorcontrib>Liu, Jiao</creatorcontrib><creatorcontrib>Hu, Xin-Wen</creatorcontrib><creatorcontrib>Guo, Jian-Chun</creatorcontrib><title>Isolation and Characterization of Ftsz Genes in Cassava</title><title>Genes</title><addtitle>Genes (Basel)</addtitle><description>The filamenting temperature-sensitive Z proteins (FtsZs) play an important role in plastid division. In this study, three FtsZ genes were isolated from the cassava genome, and named MeFtsZ1, MeFtsZ2-1, and MeFtsZ2-2, respectively. Based on phylogeny, the MeFtsZs were classified into two groups (FtsZ1 and FtsZ2). MeFtsZ1 with a putative signal peptide at N-terminal, has six exons, and is classed to FtsZ1 clade. MeFtsZ2-1 and MeFtsZ2-2 without a putative signal peptide, have seven exons, and are classed to FtsZ2 clade. Subcellular localization found that all the three MeFtsZs could locate in chloroplasts and form a ring in chloroplastids. Structure analysis found that all MeFtsZ proteins contain a conserved guanosine triphosphatase (GTPase) domain in favor of generate contractile force for cassava plastid division. The expression profiles of MeFtsZ genes by quantitative reverse transcription-PCR (qRT-PCR) analysis in photosynthetic and non-photosynthetic tissues found that all of the MeFtsZ genes had higher expression levels in photosynthetic tissues, especially in younger leaves, and lower expression levels in the non-photosynthetic tissues. During cassava storage root development, the expressions of MeFtsZ2-1 and MeFtsZ2-2 were comparatively higher than MeFtsZ1. The transformed Arabidopsis of MeFtsZ2-1 and MeFtsZ2-2 contained abnormally shape, fewer number, and larger volume chloroplasts. Phytohormones were involved in regulating the expressions of MeFtsZ genes. Therefore, we deduced that all of the
play an important role in chloroplast division, and that
2 (2-1, 2-2) might be involved in amyloplast division and regulated by phytohormones during cassava storage root development.</description><subject>Cassava</subject><subject>Chloroplasts</subject><subject>Contractility</subject><subject>Exons</subject><subject>Genomes</subject><subject>Guanosine</subject><subject>Guanosine triphosphatases</subject><subject>Localization</subject><subject>Phylogeny</subject><subject>Reverse transcription</subject><subject>Triphosphatase</subject><issn>2073-4425</issn><issn>2073-4425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1LAzEQhoMottTePMuCFw-uTr5MchFksbVQ8KLnkN3Ntlu2m5psC_bXm9JaqnOZYebhnRlehK4xPFCq4HFmWxskJkAVPkN9AoKmjBF-flL30DCEBcRgQAD4JeoRRRgTFPpITIJrTFe7NjFtmWRz403RWV9v901XJaMubJPxblFSt0lmQjAbc4UuKtMEOzzkAfocvX5kb-n0fTzJXqZpwTDpUllYwSsBhOXCijKvIM_BACV5yTCNPV4yqypJmSkop1gyXkpWgqoqwFwYOkDPe93VOl_asrBt502jV75eGv-tnan130lbz_XMbTQXTApQUeDuIODd19qGTi_rUNimMa1166CxEkJIEOIporf_0IVb-za-FykpuWIKs0jd76nCuxC8rY7HYNA7U_SpKRG_OX3gCP9aQH8AsgmHdw</recordid><startdate>20171215</startdate><enddate>20171215</enddate><creator>Geng, Meng-Ting</creator><creator>Min, Yi</creator><creator>Yao, Yuan</creator><creator>Chen, Xia</creator><creator>Fan, Jie</creator><creator>Yuan, Shuai</creator><creator>Wang, Lei</creator><creator>Sun, Chong</creator><creator>Zhang, Fan</creator><creator>Shang, Lu</creator><creator>Wang, Yun-Lin</creator><creator>Li, Rui-Mei</creator><creator>Fu, Shao-Ping</creator><creator>Duan, Rui-Jun</creator><creator>Liu, Jiao</creator><creator>Hu, Xin-Wen</creator><creator>Guo, Jian-Chun</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1791-0433</orcidid></search><sort><creationdate>20171215</creationdate><title>Isolation and Characterization of Ftsz Genes in Cassava</title><author>Geng, Meng-Ting ; 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In this study, three FtsZ genes were isolated from the cassava genome, and named MeFtsZ1, MeFtsZ2-1, and MeFtsZ2-2, respectively. Based on phylogeny, the MeFtsZs were classified into two groups (FtsZ1 and FtsZ2). MeFtsZ1 with a putative signal peptide at N-terminal, has six exons, and is classed to FtsZ1 clade. MeFtsZ2-1 and MeFtsZ2-2 without a putative signal peptide, have seven exons, and are classed to FtsZ2 clade. Subcellular localization found that all the three MeFtsZs could locate in chloroplasts and form a ring in chloroplastids. Structure analysis found that all MeFtsZ proteins contain a conserved guanosine triphosphatase (GTPase) domain in favor of generate contractile force for cassava plastid division. The expression profiles of MeFtsZ genes by quantitative reverse transcription-PCR (qRT-PCR) analysis in photosynthetic and non-photosynthetic tissues found that all of the MeFtsZ genes had higher expression levels in photosynthetic tissues, especially in younger leaves, and lower expression levels in the non-photosynthetic tissues. During cassava storage root development, the expressions of MeFtsZ2-1 and MeFtsZ2-2 were comparatively higher than MeFtsZ1. The transformed Arabidopsis of MeFtsZ2-1 and MeFtsZ2-2 contained abnormally shape, fewer number, and larger volume chloroplasts. Phytohormones were involved in regulating the expressions of MeFtsZ genes. Therefore, we deduced that all of the
play an important role in chloroplast division, and that
2 (2-1, 2-2) might be involved in amyloplast division and regulated by phytohormones during cassava storage root development.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>29244730</pmid><doi>10.3390/genes8120391</doi><orcidid>https://orcid.org/0000-0003-1791-0433</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Cassava Chloroplasts Contractility Exons Genomes Guanosine Guanosine triphosphatases Localization Phylogeny Reverse transcription Triphosphatase |
| title | Isolation and Characterization of Ftsz Genes in Cassava |
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