Gene Silencing of Argonaute5 Negatively Affects the Establishment of the Legume-Rhizobia Symbiosis
The establishment of the symbiosis between legumes and nitrogen-fixing rhizobia is finely regulated at the transcriptional, posttranscriptional and posttranslational levels. Argonaute5 (AGO5), a protein involved in RNA silencing, can bind both viral RNAs and microRNAs to control plant-microbe intera...
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Veröffentlicht in: | Genes 2017-11, Vol.8 (12), p.352 |
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creator | Reyero-Saavedra, María Del Rocio Qiao, Zhenzhen Sánchez-Correa, María Del Socorro Díaz-Pineda, M Enrique Reyes, Jose L Covarrubias, Alejandra A Libault, Marc Valdés-López, Oswaldo |
description | The establishment of the symbiosis between legumes and nitrogen-fixing rhizobia is finely regulated at the transcriptional, posttranscriptional and posttranslational levels. Argonaute5 (AGO5), a protein involved in RNA silencing, can bind both viral RNAs and microRNAs to control plant-microbe interactions and plant physiology. For instance, AGO5 regulates the systemic resistance of Arabidopsis against Potato Virus X as well as the pigmentation of soybean (
) seeds. Here, we show that AGO5 is also playing a central role in legume nodulation based on its preferential expression in common bean (
) and soybean roots and nodules. We also report that the expression of AGO5 is induced after 1 h of inoculation with rhizobia. Down-regulation of
gene in
and
causes diminished root hair curling, reduces nodule formation and interferes with the induction of three critical symbiotic genes: Nuclear Factor Y-B (
), Nodule Inception (
) and Flotillin2 (
). Our findings provide evidence that the common bean and soybean
genes play an essential role in the establishment of the symbiosis with rhizobia. |
doi_str_mv | 10.3390/genes8120352 |
format | Article |
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) seeds. Here, we show that AGO5 is also playing a central role in legume nodulation based on its preferential expression in common bean (
) and soybean roots and nodules. We also report that the expression of AGO5 is induced after 1 h of inoculation with rhizobia. Down-regulation of
gene in
and
causes diminished root hair curling, reduces nodule formation and interferes with the induction of three critical symbiotic genes: Nuclear Factor Y-B (
), Nodule Inception (
) and Flotillin2 (
). Our findings provide evidence that the common bean and soybean
genes play an essential role in the establishment of the symbiosis with rhizobia.</description><identifier>ISSN: 2073-4425</identifier><identifier>EISSN: 2073-4425</identifier><identifier>DOI: 10.3390/genes8120352</identifier><identifier>PMID: 29182547</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Disease resistance ; Gene silencing ; Genes ; Glycine max ; Inoculation ; Legumes ; miRNA ; Nodulation ; Phaseolus vulgaris ; Pigmentation ; Post-transcription ; RNA-mediated interference ; Root nodules ; Seeds ; Soybeans ; Symbiosis</subject><ispartof>Genes, 2017-11, Vol.8 (12), p.352</ispartof><rights>Copyright MDPI AG 2017</rights><rights>2017 by the authors. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-a3b7094bca738f4e18499b64d70ad6220b51c6152e68c583b51dbe6b71fa90d33</citedby><cites>FETCH-LOGICAL-c412t-a3b7094bca738f4e18499b64d70ad6220b51c6152e68c583b51dbe6b71fa90d33</cites><orcidid>0000-0001-5129-9741</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748670/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748670/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29182547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reyero-Saavedra, María Del Rocio</creatorcontrib><creatorcontrib>Qiao, Zhenzhen</creatorcontrib><creatorcontrib>Sánchez-Correa, María Del Socorro</creatorcontrib><creatorcontrib>Díaz-Pineda, M Enrique</creatorcontrib><creatorcontrib>Reyes, Jose L</creatorcontrib><creatorcontrib>Covarrubias, Alejandra A</creatorcontrib><creatorcontrib>Libault, Marc</creatorcontrib><creatorcontrib>Valdés-López, Oswaldo</creatorcontrib><title>Gene Silencing of Argonaute5 Negatively Affects the Establishment of the Legume-Rhizobia Symbiosis</title><title>Genes</title><addtitle>Genes (Basel)</addtitle><description>The establishment of the symbiosis between legumes and nitrogen-fixing rhizobia is finely regulated at the transcriptional, posttranscriptional and posttranslational levels. Argonaute5 (AGO5), a protein involved in RNA silencing, can bind both viral RNAs and microRNAs to control plant-microbe interactions and plant physiology. For instance, AGO5 regulates the systemic resistance of Arabidopsis against Potato Virus X as well as the pigmentation of soybean (
) seeds. Here, we show that AGO5 is also playing a central role in legume nodulation based on its preferential expression in common bean (
) and soybean roots and nodules. We also report that the expression of AGO5 is induced after 1 h of inoculation with rhizobia. Down-regulation of
gene in
and
causes diminished root hair curling, reduces nodule formation and interferes with the induction of three critical symbiotic genes: Nuclear Factor Y-B (
), Nodule Inception (
) and Flotillin2 (
). Our findings provide evidence that the common bean and soybean
genes play an essential role in the establishment of the symbiosis with rhizobia.</description><subject>Disease resistance</subject><subject>Gene silencing</subject><subject>Genes</subject><subject>Glycine max</subject><subject>Inoculation</subject><subject>Legumes</subject><subject>miRNA</subject><subject>Nodulation</subject><subject>Phaseolus vulgaris</subject><subject>Pigmentation</subject><subject>Post-transcription</subject><subject>RNA-mediated interference</subject><subject>Root nodules</subject><subject>Seeds</subject><subject>Soybeans</subject><subject>Symbiosis</subject><issn>2073-4425</issn><issn>2073-4425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkd1L5DAUxYO4rKK-7bMUfPHBaj6b9EUYBr9g2AXHfQ5JetuJtI02rTD-9WZwVmbNy01yf_dwLgehXwRfMlbiqwZ6iIpQzATdQ4cUS5ZzTsX-zv0AncT4jNPhmGIsfqIDWhJFBZeHyN4lhWzpW-id75ss1NlsaEJvphFE9hsaM_o3aNfZrK7BjTEbV5DdxNHY1sdVB_24Gdl8LqCZOsgfV_49WG-y5bqzPkQfj9GP2rQRTrb1CP29vXma3-eLP3cP89kid5zQMTfMSlxy64xkquZAFC9LW_BKYlMVlGIriCuIoFAoJxRLz8pCYSWpTYkrxo7Q9afuy2Q7qFzyNphWvwy-M8NaB-P1_53er3QT3rSQXBUSJ4HzrcAQXieIo-58dNC2pocwRU1KiamUyWVCz76hz2Ea-rReopQSZUFYkaiLT8oNIcYB6i8zBOtNfno3v4Sf7i7wBf9Li30A96aXFA</recordid><startdate>20171128</startdate><enddate>20171128</enddate><creator>Reyero-Saavedra, María Del Rocio</creator><creator>Qiao, Zhenzhen</creator><creator>Sánchez-Correa, María Del Socorro</creator><creator>Díaz-Pineda, M Enrique</creator><creator>Reyes, Jose L</creator><creator>Covarrubias, Alejandra A</creator><creator>Libault, Marc</creator><creator>Valdés-López, Oswaldo</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5129-9741</orcidid></search><sort><creationdate>20171128</creationdate><title>Gene Silencing of Argonaute5 Negatively Affects the Establishment of the Legume-Rhizobia Symbiosis</title><author>Reyero-Saavedra, María Del Rocio ; Qiao, Zhenzhen ; Sánchez-Correa, María Del Socorro ; Díaz-Pineda, M Enrique ; Reyes, Jose L ; Covarrubias, Alejandra A ; Libault, Marc ; Valdés-López, Oswaldo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-a3b7094bca738f4e18499b64d70ad6220b51c6152e68c583b51dbe6b71fa90d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Disease resistance</topic><topic>Gene silencing</topic><topic>Genes</topic><topic>Glycine max</topic><topic>Inoculation</topic><topic>Legumes</topic><topic>miRNA</topic><topic>Nodulation</topic><topic>Phaseolus vulgaris</topic><topic>Pigmentation</topic><topic>Post-transcription</topic><topic>RNA-mediated interference</topic><topic>Root nodules</topic><topic>Seeds</topic><topic>Soybeans</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reyero-Saavedra, María Del Rocio</creatorcontrib><creatorcontrib>Qiao, Zhenzhen</creatorcontrib><creatorcontrib>Sánchez-Correa, María Del Socorro</creatorcontrib><creatorcontrib>Díaz-Pineda, M Enrique</creatorcontrib><creatorcontrib>Reyes, Jose L</creatorcontrib><creatorcontrib>Covarrubias, Alejandra A</creatorcontrib><creatorcontrib>Libault, Marc</creatorcontrib><creatorcontrib>Valdés-López, Oswaldo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reyero-Saavedra, María Del Rocio</au><au>Qiao, Zhenzhen</au><au>Sánchez-Correa, María Del Socorro</au><au>Díaz-Pineda, M Enrique</au><au>Reyes, Jose L</au><au>Covarrubias, Alejandra A</au><au>Libault, Marc</au><au>Valdés-López, Oswaldo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene Silencing of Argonaute5 Negatively Affects the Establishment of the Legume-Rhizobia Symbiosis</atitle><jtitle>Genes</jtitle><addtitle>Genes (Basel)</addtitle><date>2017-11-28</date><risdate>2017</risdate><volume>8</volume><issue>12</issue><spage>352</spage><pages>352-</pages><issn>2073-4425</issn><eissn>2073-4425</eissn><abstract>The establishment of the symbiosis between legumes and nitrogen-fixing rhizobia is finely regulated at the transcriptional, posttranscriptional and posttranslational levels. Argonaute5 (AGO5), a protein involved in RNA silencing, can bind both viral RNAs and microRNAs to control plant-microbe interactions and plant physiology. For instance, AGO5 regulates the systemic resistance of Arabidopsis against Potato Virus X as well as the pigmentation of soybean (
) seeds. Here, we show that AGO5 is also playing a central role in legume nodulation based on its preferential expression in common bean (
) and soybean roots and nodules. We also report that the expression of AGO5 is induced after 1 h of inoculation with rhizobia. Down-regulation of
gene in
and
causes diminished root hair curling, reduces nodule formation and interferes with the induction of three critical symbiotic genes: Nuclear Factor Y-B (
), Nodule Inception (
) and Flotillin2 (
). Our findings provide evidence that the common bean and soybean
genes play an essential role in the establishment of the symbiosis with rhizobia.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>29182547</pmid><doi>10.3390/genes8120352</doi><orcidid>https://orcid.org/0000-0001-5129-9741</orcidid><oa>free_for_read</oa></addata></record> |
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source | MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
subjects | Disease resistance Gene silencing Genes Glycine max Inoculation Legumes miRNA Nodulation Phaseolus vulgaris Pigmentation Post-transcription RNA-mediated interference Root nodules Seeds Soybeans Symbiosis |
title | Gene Silencing of Argonaute5 Negatively Affects the Establishment of the Legume-Rhizobia Symbiosis |
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