Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats
The performance of a rapid immunochromatographic test for the detection of Dirofilaria immitis antigens (Speed Diro™; BVT-Virbac, France) was assessed in 49 experimentally infected dogs and in 244 naturally infected animals; 142 dogs and 102 cats. In experimentally infected dogs, Speed Diro™ showed...
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| Veröffentlicht in: | Parasitology research (1987) 2018-01, Vol.117 (1), p.31-34 |
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| container_title | Parasitology research (1987) |
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| creator | Genchi, Marco Mangia, Carlo Ferrari, Nicola Loukeri, Sofia |
| description | The performance of a rapid immunochromatographic test for the detection of
Dirofilaria immitis
antigens (Speed Diro™; BVT-Virbac, France) was assessed in 49 experimentally infected dogs and in 244 naturally infected animals; 142 dogs and 102 cats. In experimentally infected dogs, Speed Diro™ showed a sensitivity of 90.9% in dogs infected with one adult female worm and 100% in dogs infected with more than one female worm. Specificity was 100%. For naturally infected dogs, the Knott test and PetChek
®
HTWM PF served as reference methods for microfilaremia and antigenemia, respectively. All microfilaemic dogs (55/142) were positive with Speed Diro™. Importantly, none of the 21 dogs infected with
D. repens
were positive. The results of Speed Diro™ for the detection of antigenemia were compared with two in-house tests, SNAP
®
HTWM and Witness
®
Dirofilaria, and all three tests were 100% specific and sensitive in comparison to PetChek
®
HTWM PF. For the evaluation of feline samples, 102 cats were examined by echocardiography. Sera from 87 heartworm-infected cats were tested by Speed Diro™ and SNAP
®
HTWM. The results of Speed Diro™ were equivalent to SNAP
®
HTWM, with a sensitivity of 98.9% and a specificity of 100%. |
| doi_str_mv | 10.1007/s00436-017-5709-2 |
| format | Article |
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Dirofilaria immitis
antigens (Speed Diro™; BVT-Virbac, France) was assessed in 49 experimentally infected dogs and in 244 naturally infected animals; 142 dogs and 102 cats. In experimentally infected dogs, Speed Diro™ showed a sensitivity of 90.9% in dogs infected with one adult female worm and 100% in dogs infected with more than one female worm. Specificity was 100%. For naturally infected dogs, the Knott test and PetChek
®
HTWM PF served as reference methods for microfilaremia and antigenemia, respectively. All microfilaemic dogs (55/142) were positive with Speed Diro™. Importantly, none of the 21 dogs infected with
D. repens
were positive. The results of Speed Diro™ for the detection of antigenemia were compared with two in-house tests, SNAP
®
HTWM and Witness
®
Dirofilaria, and all three tests were 100% specific and sensitive in comparison to PetChek
®
HTWM PF. For the evaluation of feline samples, 102 cats were examined by echocardiography. Sera from 87 heartworm-infected cats were tested by Speed Diro™ and SNAP
®
HTWM. The results of Speed Diro™ were equivalent to SNAP
®
HTWM, with a sensitivity of 98.9% and a specificity of 100%.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-017-5709-2</identifier><identifier>PMID: 29224180</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Antigenemia ; Antigens ; Antigens, Helminth - blood ; Biomedical and Life Sciences ; Biomedicine ; Cat diseases ; Cats ; Chromatography ; Diagnosis ; Dirofilaria immitis ; Dirofilaria immitis - immunology ; Dirofilariasis - diagnosis ; Dog diseases ; Dog Diseases - diagnosis ; Dog Diseases - parasitology ; Dogs ; Echocardiography ; Female ; France ; Health aspects ; Immunochromatography - methods ; Immunochromatography - veterinary ; Immunology ; Male ; Medical Microbiology ; Methods ; Microbiology ; Review ; Roundworms ; Sensitivity and Specificity</subject><ispartof>Parasitology research (1987), 2018-01, Vol.117 (1), p.31-34</ispartof><rights>The Author(s) 2017</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-d6e52aa72c30bea3764dff36b563bb5eed158fd3e282f6b92e13f28955b546d3</citedby><cites>FETCH-LOGICAL-c509t-d6e52aa72c30bea3764dff36b563bb5eed158fd3e282f6b92e13f28955b546d3</cites><orcidid>0000-0001-5339-905X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-017-5709-2$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-017-5709-2$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29224180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Genchi, Marco</creatorcontrib><creatorcontrib>Mangia, Carlo</creatorcontrib><creatorcontrib>Ferrari, Nicola</creatorcontrib><creatorcontrib>Loukeri, Sofia</creatorcontrib><title>Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>The performance of a rapid immunochromatographic test for the detection of
Dirofilaria immitis
antigens (Speed Diro™; BVT-Virbac, France) was assessed in 49 experimentally infected dogs and in 244 naturally infected animals; 142 dogs and 102 cats. In experimentally infected dogs, Speed Diro™ showed a sensitivity of 90.9% in dogs infected with one adult female worm and 100% in dogs infected with more than one female worm. Specificity was 100%. For naturally infected dogs, the Knott test and PetChek
®
HTWM PF served as reference methods for microfilaremia and antigenemia, respectively. All microfilaemic dogs (55/142) were positive with Speed Diro™. Importantly, none of the 21 dogs infected with
D. repens
were positive. The results of Speed Diro™ for the detection of antigenemia were compared with two in-house tests, SNAP
®
HTWM and Witness
®
Dirofilaria, and all three tests were 100% specific and sensitive in comparison to PetChek
®
HTWM PF. For the evaluation of feline samples, 102 cats were examined by echocardiography. Sera from 87 heartworm-infected cats were tested by Speed Diro™ and SNAP
®
HTWM. The results of Speed Diro™ were equivalent to SNAP
®
HTWM, with a sensitivity of 98.9% and a specificity of 100%.</description><subject>Animals</subject><subject>Antigenemia</subject><subject>Antigens</subject><subject>Antigens, Helminth - blood</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cat diseases</subject><subject>Cats</subject><subject>Chromatography</subject><subject>Diagnosis</subject><subject>Dirofilaria immitis</subject><subject>Dirofilaria immitis - immunology</subject><subject>Dirofilariasis - diagnosis</subject><subject>Dog diseases</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - parasitology</subject><subject>Dogs</subject><subject>Echocardiography</subject><subject>Female</subject><subject>France</subject><subject>Health aspects</subject><subject>Immunochromatography - methods</subject><subject>Immunochromatography - veterinary</subject><subject>Immunology</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Review</subject><subject>Roundworms</subject><subject>Sensitivity and Specificity</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp1Uk1rHSEUldLSvL72B3RThG7SxaR-jPOxKYQk_YBAN9mLo9d5hhl9VSchy_7z-jJJSAvFheI953jv8SD0npITSkj7ORFS86YitK1ES_qKvUAbWnNW0V6Il2hD-nImlPIj9Cala1KATV2_RkesZ6ymHdmg3xc3alpUdsHjYLHCUe2dwW6eFx_0LoZZ5TCWy53TOEPK2IaI8w6wgQz6kTeFWzws0YDH5y4G6yYVnTrIuOwSPt6Bivk2xPkTdh6bMCasvMFa5fQWvbJqSvDuYd-iq68XV2ffq8uf336cnV5WWpA-V6YBwZRqmeZkAMXLJMZa3gyi4cMgAAwVnTUcWMdsM_QMKLesK0YMom4M36Ivq-x-GWYwGnyOapL76GYV72RQTv5d8W4nx3AjRVt3xeUicPwgEMOvpTghZ5c0TJPyEJYkad8K0XPS9QX68R_odViiL9MVVMdryht6QJ2sqFFNIJ23obyryzIwOx08FBdBngpGSXffwxbRlaBjSCmCfeqeEnkIhFwDIcs_y0MgJCucD8_HfmI8JqAA2ApIpeRHiM96_a_qHyv3wyo</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Genchi, Marco</creator><creator>Mangia, Carlo</creator><creator>Ferrari, Nicola</creator><creator>Loukeri, Sofia</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5339-905X</orcidid></search><sort><creationdate>20180101</creationdate><title>Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats</title><author>Genchi, Marco ; Mangia, Carlo ; Ferrari, Nicola ; Loukeri, Sofia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-d6e52aa72c30bea3764dff36b563bb5eed158fd3e282f6b92e13f28955b546d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antigenemia</topic><topic>Antigens</topic><topic>Antigens, Helminth - blood</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cat diseases</topic><topic>Cats</topic><topic>Chromatography</topic><topic>Diagnosis</topic><topic>Dirofilaria immitis</topic><topic>Dirofilaria immitis - immunology</topic><topic>Dirofilariasis - diagnosis</topic><topic>Dog diseases</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - parasitology</topic><topic>Dogs</topic><topic>Echocardiography</topic><topic>Female</topic><topic>France</topic><topic>Health aspects</topic><topic>Immunochromatography - methods</topic><topic>Immunochromatography - veterinary</topic><topic>Immunology</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Review</topic><topic>Roundworms</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Genchi, Marco</creatorcontrib><creatorcontrib>Mangia, Carlo</creatorcontrib><creatorcontrib>Ferrari, Nicola</creatorcontrib><creatorcontrib>Loukeri, Sofia</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Genchi, Marco</au><au>Mangia, Carlo</au><au>Ferrari, Nicola</au><au>Loukeri, Sofia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2018-01-01</date><risdate>2018</risdate><volume>117</volume><issue>1</issue><spage>31</spage><epage>34</epage><pages>31-34</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>The performance of a rapid immunochromatographic test for the detection of
Dirofilaria immitis
antigens (Speed Diro™; BVT-Virbac, France) was assessed in 49 experimentally infected dogs and in 244 naturally infected animals; 142 dogs and 102 cats. In experimentally infected dogs, Speed Diro™ showed a sensitivity of 90.9% in dogs infected with one adult female worm and 100% in dogs infected with more than one female worm. Specificity was 100%. For naturally infected dogs, the Knott test and PetChek
®
HTWM PF served as reference methods for microfilaremia and antigenemia, respectively. All microfilaemic dogs (55/142) were positive with Speed Diro™. Importantly, none of the 21 dogs infected with
D. repens
were positive. The results of Speed Diro™ for the detection of antigenemia were compared with two in-house tests, SNAP
®
HTWM and Witness
®
Dirofilaria, and all three tests were 100% specific and sensitive in comparison to PetChek
®
HTWM PF. For the evaluation of feline samples, 102 cats were examined by echocardiography. Sera from 87 heartworm-infected cats were tested by Speed Diro™ and SNAP
®
HTWM. The results of Speed Diro™ were equivalent to SNAP
®
HTWM, with a sensitivity of 98.9% and a specificity of 100%.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29224180</pmid><doi>10.1007/s00436-017-5709-2</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0001-5339-905X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Parasitology research (1987), 2018-01, Vol.117 (1), p.31-34 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5748436 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Antigenemia Antigens Antigens, Helminth - blood Biomedical and Life Sciences Biomedicine Cat diseases Cats Chromatography Diagnosis Dirofilaria immitis Dirofilaria immitis - immunology Dirofilariasis - diagnosis Dog diseases Dog Diseases - diagnosis Dog Diseases - parasitology Dogs Echocardiography Female France Health aspects Immunochromatography - methods Immunochromatography - veterinary Immunology Male Medical Microbiology Methods Microbiology Review Roundworms Sensitivity and Specificity |
| title | Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats |
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