Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets
The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first mor...
Gespeichert in:
| Veröffentlicht in: | Journal of Veterinary Medical Science 2017, Vol.79(12), pp.1960-1967 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1967 |
|---|---|
| container_issue | 12 |
| container_start_page | 1960 |
| container_title | Journal of Veterinary Medical Science |
| container_volume | 79 |
| creator | TAKAHASHI, Naoki TANGKAWATTANA, Prasarn OOTOMO, Yoshiki HIROSE, Takuya MINAGUCHI, Jun UEDA, Hiromi YAMADA, Michi TAKEHANA, Kazushige |
| description | The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first morphometric analysis to describe the characteristics of tenocytes in the metacarpal superficial digital flexor tendon of 0-, 10- and 20-day-old piglets. In the present study, hydrochloric acid/collagenase digestion was applied to remove the interstitial connective tissue to obtain clear visualization of intact tenocytes and their cytoplasmic processes (Cp). Then, the morphometry of the tenocytes was investigated by optical and electron microscopy. The mean ± SE values of the fascicle area, number of tenocytes/fascicle, cell density, number of Cp/tenocyte, length of Cp, and thickness of Cp were compared among the three age groups. Significant differences (judged at P |
| doi_str_mv | 10.1292/jvms.17-0436 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5745172</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1956089741</sourcerecordid><originalsourceid>FETCH-LOGICAL-c707t-36ea9c0006ff4c7b25dfa8831f2a6548551fbdf2b231a5ad0e621a3c6c2a59943</originalsourceid><addsrcrecordid>eNpdkc-P1CAYhonRuLOrN8-miRcPduVHgeFiYia6mqzxolcJQz86TNpSga7Ofy-160S98B2-hycvvAg9I_iaUEVfH--GdE1kjRsmHqANYY2sZcPUQ7TBiohaUo4v0GVKR4wpaYR6jC6owhJLwTfo26cQp0MYIEdvKzOa_pR8qoKruhh--LGrMozBnjKkyo9VPkCV5gmi89abvmp953OZroefIS5sG8bl9uS7HnJ6gh450yd4ej-v0Nf3777sPtS3n28-7t7e1rbkyDUTYJTFGAvnGiv3lLfObLeMOGoEb7acE7dvHd1TRgw3LQZBiWFWWGq4Ug27Qm9W7zTvB2gtjDmaXk_RDyaedDBe_7sZ_UF34U5z2XAiaRG8vBfE8H2GlPXgk4W-NyOEOWmiuMBbJRtS0Bf_occwx_JzSVPKlCAl8SJ8tVI2hpQiuHMYgvVSnF6K00TqpbiCP__7AWf4T1MF2K3AMWXTwRkwMXvbw2qTqqh_n6v2vLUHEzWM7BfvB67Z</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2239616542</pqid></control><display><type>article</type><title>Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets</title><source>J-STAGE Free</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>TAKAHASHI, Naoki ; TANGKAWATTANA, Prasarn ; OOTOMO, Yoshiki ; HIROSE, Takuya ; MINAGUCHI, Jun ; UEDA, Hiromi ; YAMADA, Michi ; TAKEHANA, Kazushige</creator><creatorcontrib>TAKAHASHI, Naoki ; TANGKAWATTANA, Prasarn ; OOTOMO, Yoshiki ; HIROSE, Takuya ; MINAGUCHI, Jun ; UEDA, Hiromi ; YAMADA, Michi ; TAKEHANA, Kazushige</creatorcontrib><description>The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first morphometric analysis to describe the characteristics of tenocytes in the metacarpal superficial digital flexor tendon of 0-, 10- and 20-day-old piglets. In the present study, hydrochloric acid/collagenase digestion was applied to remove the interstitial connective tissue to obtain clear visualization of intact tenocytes and their cytoplasmic processes (Cp). Then, the morphometry of the tenocytes was investigated by optical and electron microscopy. The mean ± SE values of the fascicle area, number of tenocytes/fascicle, cell density, number of Cp/tenocyte, length of Cp, and thickness of Cp were compared among the three age groups. Significant differences (judged at P<0.05) were found in almost all morphometric aspects among the age groups, except for the number of Cp/cell (P=0.545) and thickness of the Cp (P=0.105). A decrease of cell density corresponded with an increase in the length of the Cp, which were extended to connect either with the Cp of the other tenocytes or the surrounding endotendineum. Moreover, an increase of the fascicle area reflected the increase in tendon diameter. The revealed morphometric characteristics are thus the outcome of tendon growth.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.17-0436</identifier><identifier>PMID: 29070765</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Anatomy ; Cell density ; Collagen ; Collagenase ; Connective tissues ; Electron microscopy ; Growth patterns ; Hydrochloric acid ; Metacarpal ; Morphometry ; piglets ; superficial digital flexor tendon ; Tendons ; tenocyte</subject><ispartof>Journal of Veterinary Medical Science, 2017, Vol.79(12), pp.1960-1967</ispartof><rights>2017 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency Dec 2017</rights><rights>2017 The Japanese Society of Veterinary Science 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c707t-36ea9c0006ff4c7b25dfa8831f2a6548551fbdf2b231a5ad0e621a3c6c2a59943</citedby><cites>FETCH-LOGICAL-c707t-36ea9c0006ff4c7b25dfa8831f2a6548551fbdf2b231a5ad0e621a3c6c2a59943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745172/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5745172/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29070765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TAKAHASHI, Naoki</creatorcontrib><creatorcontrib>TANGKAWATTANA, Prasarn</creatorcontrib><creatorcontrib>OOTOMO, Yoshiki</creatorcontrib><creatorcontrib>HIROSE, Takuya</creatorcontrib><creatorcontrib>MINAGUCHI, Jun</creatorcontrib><creatorcontrib>UEDA, Hiromi</creatorcontrib><creatorcontrib>YAMADA, Michi</creatorcontrib><creatorcontrib>TAKEHANA, Kazushige</creatorcontrib><title>Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first morphometric analysis to describe the characteristics of tenocytes in the metacarpal superficial digital flexor tendon of 0-, 10- and 20-day-old piglets. In the present study, hydrochloric acid/collagenase digestion was applied to remove the interstitial connective tissue to obtain clear visualization of intact tenocytes and their cytoplasmic processes (Cp). Then, the morphometry of the tenocytes was investigated by optical and electron microscopy. The mean ± SE values of the fascicle area, number of tenocytes/fascicle, cell density, number of Cp/tenocyte, length of Cp, and thickness of Cp were compared among the three age groups. Significant differences (judged at P<0.05) were found in almost all morphometric aspects among the age groups, except for the number of Cp/cell (P=0.545) and thickness of the Cp (P=0.105). A decrease of cell density corresponded with an increase in the length of the Cp, which were extended to connect either with the Cp of the other tenocytes or the surrounding endotendineum. Moreover, an increase of the fascicle area reflected the increase in tendon diameter. The revealed morphometric characteristics are thus the outcome of tendon growth.</description><subject>Anatomy</subject><subject>Cell density</subject><subject>Collagen</subject><subject>Collagenase</subject><subject>Connective tissues</subject><subject>Electron microscopy</subject><subject>Growth patterns</subject><subject>Hydrochloric acid</subject><subject>Metacarpal</subject><subject>Morphometry</subject><subject>piglets</subject><subject>superficial digital flexor tendon</subject><subject>Tendons</subject><subject>tenocyte</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpdkc-P1CAYhonRuLOrN8-miRcPduVHgeFiYia6mqzxolcJQz86TNpSga7Ofy-160S98B2-hycvvAg9I_iaUEVfH--GdE1kjRsmHqANYY2sZcPUQ7TBiohaUo4v0GVKR4wpaYR6jC6owhJLwTfo26cQp0MYIEdvKzOa_pR8qoKruhh--LGrMozBnjKkyo9VPkCV5gmi89abvmp953OZroefIS5sG8bl9uS7HnJ6gh450yd4ej-v0Nf3777sPtS3n28-7t7e1rbkyDUTYJTFGAvnGiv3lLfObLeMOGoEb7acE7dvHd1TRgw3LQZBiWFWWGq4Ug27Qm9W7zTvB2gtjDmaXk_RDyaedDBe_7sZ_UF34U5z2XAiaRG8vBfE8H2GlPXgk4W-NyOEOWmiuMBbJRtS0Bf_occwx_JzSVPKlCAl8SJ8tVI2hpQiuHMYgvVSnF6K00TqpbiCP__7AWf4T1MF2K3AMWXTwRkwMXvbw2qTqqh_n6v2vLUHEzWM7BfvB67Z</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>TAKAHASHI, Naoki</creator><creator>TANGKAWATTANA, Prasarn</creator><creator>OOTOMO, Yoshiki</creator><creator>HIROSE, Takuya</creator><creator>MINAGUCHI, Jun</creator><creator>UEDA, Hiromi</creator><creator>YAMADA, Michi</creator><creator>TAKEHANA, Kazushige</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2017</creationdate><title>Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets</title><author>TAKAHASHI, Naoki ; TANGKAWATTANA, Prasarn ; OOTOMO, Yoshiki ; HIROSE, Takuya ; MINAGUCHI, Jun ; UEDA, Hiromi ; YAMADA, Michi ; TAKEHANA, Kazushige</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c707t-36ea9c0006ff4c7b25dfa8831f2a6548551fbdf2b231a5ad0e621a3c6c2a59943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Anatomy</topic><topic>Cell density</topic><topic>Collagen</topic><topic>Collagenase</topic><topic>Connective tissues</topic><topic>Electron microscopy</topic><topic>Growth patterns</topic><topic>Hydrochloric acid</topic><topic>Metacarpal</topic><topic>Morphometry</topic><topic>piglets</topic><topic>superficial digital flexor tendon</topic><topic>Tendons</topic><topic>tenocyte</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAKAHASHI, Naoki</creatorcontrib><creatorcontrib>TANGKAWATTANA, Prasarn</creatorcontrib><creatorcontrib>OOTOMO, Yoshiki</creatorcontrib><creatorcontrib>HIROSE, Takuya</creatorcontrib><creatorcontrib>MINAGUCHI, Jun</creatorcontrib><creatorcontrib>UEDA, Hiromi</creatorcontrib><creatorcontrib>YAMADA, Michi</creatorcontrib><creatorcontrib>TAKEHANA, Kazushige</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAKAHASHI, Naoki</au><au>TANGKAWATTANA, Prasarn</au><au>OOTOMO, Yoshiki</au><au>HIROSE, Takuya</au><au>MINAGUCHI, Jun</au><au>UEDA, Hiromi</au><au>YAMADA, Michi</au><au>TAKEHANA, Kazushige</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2017</date><risdate>2017</risdate><volume>79</volume><issue>12</issue><spage>1960</spage><epage>1967</epage><pages>1960-1967</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>The fine structures of different tendons in various animals at different ages have been studied extensively to reveal their arrangement and growth patterns. However, knowledge of the microstructures of the growing tenocytes in the tendons of piglets is still lacking. Thus, we performed the first morphometric analysis to describe the characteristics of tenocytes in the metacarpal superficial digital flexor tendon of 0-, 10- and 20-day-old piglets. In the present study, hydrochloric acid/collagenase digestion was applied to remove the interstitial connective tissue to obtain clear visualization of intact tenocytes and their cytoplasmic processes (Cp). Then, the morphometry of the tenocytes was investigated by optical and electron microscopy. The mean ± SE values of the fascicle area, number of tenocytes/fascicle, cell density, number of Cp/tenocyte, length of Cp, and thickness of Cp were compared among the three age groups. Significant differences (judged at P<0.05) were found in almost all morphometric aspects among the age groups, except for the number of Cp/cell (P=0.545) and thickness of the Cp (P=0.105). A decrease of cell density corresponded with an increase in the length of the Cp, which were extended to connect either with the Cp of the other tenocytes or the surrounding endotendineum. Moreover, an increase of the fascicle area reflected the increase in tendon diameter. The revealed morphometric characteristics are thus the outcome of tendon growth.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>29070765</pmid><doi>10.1292/jvms.17-0436</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 2017, Vol.79(12), pp.1960-1967 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5745172 |
| source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Anatomy Cell density Collagen Collagenase Connective tissues Electron microscopy Growth patterns Hydrochloric acid Metacarpal Morphometry piglets superficial digital flexor tendon Tendons tenocyte |
| title | Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T16%3A43%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Morphometric%20analysis%20of%20growing%20tenocytes%20in%20the%20superficial%20digital%20flexor%20tendon%20of%20piglets&rft.jtitle=Journal%20of%20Veterinary%20Medical%20Science&rft.au=TAKAHASHI,%20Naoki&rft.date=2017&rft.volume=79&rft.issue=12&rft.spage=1960&rft.epage=1967&rft.pages=1960-1967&rft.issn=0916-7250&rft.eissn=1347-7439&rft_id=info:doi/10.1292/jvms.17-0436&rft_dat=%3Cproquest_pubme%3E1956089741%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2239616542&rft_id=info:pmid/29070765&rfr_iscdi=true |