RNA-seq analysis provide new insights into mapk signaling of apolipoproteinciii-induced inflammation in porcine vascular endothelial cells
Apolipoprotein CIII (ApoCIII) has been shown to be associated with the inflammatory response, but the mechanism of its inflammatory effects remains unclear. Because vascular endothelial cells (VECs) play a key role in the development of inflammation, the present study was performed to investigate in...
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| Veröffentlicht in: | Cell cycle (Georgetown, Tex.) Tex.), 2017-01, Vol.16 (22), p.2230-2238 |
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| creator | Yue, Yuan Jiang, Hao Yan, Shouqing Fu, Yao Liu, Chang Sun, Xulei Chai, Menglong Gao, Yan Yuan, Bao Chen, Chengzhen Dai, Lisheng Zhang, Jiabao Ding, Yu |
| description | Apolipoprotein CIII (ApoCIII) has been shown to be associated with the inflammatory response, but the mechanism of its inflammatory effects remains unclear. Because vascular endothelial cells (VECs) play a key role in the development of inflammation, the present study was performed to investigate inflammatory mechanisms induced by ApoCIII in VECs. In this study, we screened differentially expressed genes (DEGs) using RNA-sequencing. The results identified 390 up-regulated genes and 257 down-regulated genes. We performed GO functional classification and KEGG pathway analysis for DEGs. Analysis of sequencing data showed that 21 genes were related to the MAPK pathway. Finally, we investigated whether ApoCIII regulates the expression of pro-inflammatory cytokines via MAPK signaling pathway. The results showed that ApoCIII increased the expression levels of IL-6, TNF-α, VCAM-1 and ICAM-1 in VECs. ApoCIII activated the phosphorylation of ERK1/2 and p38 MAPK. An inhibitor of ERK1/2 and p38 MAPK decreased the protein levels of IL-6 and TNF-α. Our findings demonstrate that ApoCIII induces pro-inflammatory cytokine production in VECs via activation of ERK1/2 and p38 MAPK phosphorylation. |
| doi_str_mv | 10.1080/15384101.2017.1373222 |
| format | Article |
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Because vascular endothelial cells (VECs) play a key role in the development of inflammation, the present study was performed to investigate inflammatory mechanisms induced by ApoCIII in VECs. In this study, we screened differentially expressed genes (DEGs) using RNA-sequencing. The results identified 390 up-regulated genes and 257 down-regulated genes. We performed GO functional classification and KEGG pathway analysis for DEGs. Analysis of sequencing data showed that 21 genes were related to the MAPK pathway. Finally, we investigated whether ApoCIII regulates the expression of pro-inflammatory cytokines via MAPK signaling pathway. The results showed that ApoCIII increased the expression levels of IL-6, TNF-α, VCAM-1 and ICAM-1 in VECs. ApoCIII activated the phosphorylation of ERK1/2 and p38 MAPK. An inhibitor of ERK1/2 and p38 MAPK decreased the protein levels of IL-6 and TNF-α. Our findings demonstrate that ApoCIII induces pro-inflammatory cytokine production in VECs via activation of ERK1/2 and p38 MAPK phosphorylation.</description><identifier>ISSN: 1538-4101</identifier><identifier>EISSN: 1551-4005</identifier><identifier>DOI: 10.1080/15384101.2017.1373222</identifier><identifier>PMID: 28933987</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Animals ; Apolipoprotein C-III - pharmacology ; Endothelial Cells - metabolism ; Inflammation ; Interleukin-6 - metabolism ; Mitogen-Activated Protein Kinase 1 - genetics ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 - genetics ; Mitogen-Activated Protein Kinase 3 - metabolism ; Phosphorylation ; Signal Transduction - physiology ; Swine ; Tumor Necrosis Factor-alpha - metabolism ; Vascular Cell Adhesion Molecule-1 - genetics ; Vascular Cell Adhesion Molecule-1 - metabolism</subject><ispartof>Cell cycle (Georgetown, Tex.), 2017-01, Vol.16 (22), p.2230-2238</ispartof><rights>2017 Taylor & Francis Group, LLC 2017 Taylor & Francis Group, LLC</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-951802bcff2260c0bac7f102e39be4b3e8d8cef9495c4f1393f2cfcfdc35a5ce3</citedby><cites>FETCH-LOGICAL-c411t-951802bcff2260c0bac7f102e39be4b3e8d8cef9495c4f1393f2cfcfdc35a5ce3</cites><orcidid>0000-0003-3490-0755</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736343/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736343/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28933987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yue, Yuan</creatorcontrib><creatorcontrib>Jiang, Hao</creatorcontrib><creatorcontrib>Yan, Shouqing</creatorcontrib><creatorcontrib>Fu, Yao</creatorcontrib><creatorcontrib>Liu, Chang</creatorcontrib><creatorcontrib>Sun, Xulei</creatorcontrib><creatorcontrib>Chai, Menglong</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Yuan, Bao</creatorcontrib><creatorcontrib>Chen, Chengzhen</creatorcontrib><creatorcontrib>Dai, Lisheng</creatorcontrib><creatorcontrib>Zhang, Jiabao</creatorcontrib><creatorcontrib>Ding, Yu</creatorcontrib><title>RNA-seq analysis provide new insights into mapk signaling of apolipoproteinciii-induced inflammation in porcine vascular endothelial cells</title><title>Cell cycle (Georgetown, Tex.)</title><addtitle>Cell Cycle</addtitle><description>Apolipoprotein CIII (ApoCIII) has been shown to be associated with the inflammatory response, but the mechanism of its inflammatory effects remains unclear. Because vascular endothelial cells (VECs) play a key role in the development of inflammation, the present study was performed to investigate inflammatory mechanisms induced by ApoCIII in VECs. In this study, we screened differentially expressed genes (DEGs) using RNA-sequencing. The results identified 390 up-regulated genes and 257 down-regulated genes. We performed GO functional classification and KEGG pathway analysis for DEGs. Analysis of sequencing data showed that 21 genes were related to the MAPK pathway. Finally, we investigated whether ApoCIII regulates the expression of pro-inflammatory cytokines via MAPK signaling pathway. The results showed that ApoCIII increased the expression levels of IL-6, TNF-α, VCAM-1 and ICAM-1 in VECs. ApoCIII activated the phosphorylation of ERK1/2 and p38 MAPK. An inhibitor of ERK1/2 and p38 MAPK decreased the protein levels of IL-6 and TNF-α. Our findings demonstrate that ApoCIII induces pro-inflammatory cytokine production in VECs via activation of ERK1/2 and p38 MAPK phosphorylation.</description><subject>Animals</subject><subject>Apolipoprotein C-III - pharmacology</subject><subject>Endothelial Cells - metabolism</subject><subject>Inflammation</subject><subject>Interleukin-6 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 1 - genetics</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 3 - genetics</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>Phosphorylation</subject><subject>Signal Transduction - physiology</subject><subject>Swine</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Vascular Cell Adhesion Molecule-1 - genetics</subject><subject>Vascular Cell Adhesion Molecule-1 - metabolism</subject><issn>1538-4101</issn><issn>1551-4005</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkd1u1DAQhS0EoqXwCCBfcpPFP_HGvkGqqvIjVa2E4NpynPGuwbFTO1nUV-hT11G3FVzNjH3OGVsfQu8p2VAiyScquGwpoRtGaLehvOOMsRfolApBm5YQ8XLtuWxW0Ql6U8pvQpjsFH2NTphUnCvZnaL7H9fnTYFbbKIJd8UXPOV08APgCH-xj8Xv9nOpzZzwaKY_uB5UpY87nBw2Uwp-StUyg4_We9_4OCwWhupwwYyjmX2KdcBTytZHwAdT7BJMxhCHNO8heBOwhRDKW_TKmVDg3bGeoV9fLn9efGuubr5-vzi_amxL6dwoQSVhvXWOsS2xpDe2c5Qw4KqHtucgB2nBqVYJ2zrKFXfMOusGy4URFvgZ-vyYOy39CIOFOGcT9JT9aPKdTsbr_2-i3-tdOmjR8S1veQ34eAzI6XaBMuvRl_ULJkJaiqaqZVvJhVBVKh6lNqdSMrjnNZTolaN-4qhXjvrIsfo-_PvGZ9cTOP4AhHuefg</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Yue, Yuan</creator><creator>Jiang, Hao</creator><creator>Yan, Shouqing</creator><creator>Fu, Yao</creator><creator>Liu, Chang</creator><creator>Sun, Xulei</creator><creator>Chai, Menglong</creator><creator>Gao, Yan</creator><creator>Yuan, Bao</creator><creator>Chen, Chengzhen</creator><creator>Dai, Lisheng</creator><creator>Zhang, Jiabao</creator><creator>Ding, Yu</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3490-0755</orcidid></search><sort><creationdate>20170101</creationdate><title>RNA-seq analysis provide new insights into mapk signaling of apolipoproteinciii-induced inflammation in porcine vascular endothelial cells</title><author>Yue, Yuan ; Jiang, Hao ; Yan, Shouqing ; Fu, Yao ; Liu, Chang ; Sun, Xulei ; Chai, Menglong ; Gao, Yan ; Yuan, Bao ; Chen, Chengzhen ; Dai, Lisheng ; Zhang, Jiabao ; Ding, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-951802bcff2260c0bac7f102e39be4b3e8d8cef9495c4f1393f2cfcfdc35a5ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Apolipoprotein C-III - pharmacology</topic><topic>Endothelial Cells - metabolism</topic><topic>Inflammation</topic><topic>Interleukin-6 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 1 - genetics</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3 - genetics</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>Phosphorylation</topic><topic>Signal Transduction - physiology</topic><topic>Swine</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Vascular Cell Adhesion Molecule-1 - genetics</topic><topic>Vascular Cell Adhesion Molecule-1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yue, Yuan</creatorcontrib><creatorcontrib>Jiang, Hao</creatorcontrib><creatorcontrib>Yan, Shouqing</creatorcontrib><creatorcontrib>Fu, Yao</creatorcontrib><creatorcontrib>Liu, Chang</creatorcontrib><creatorcontrib>Sun, Xulei</creatorcontrib><creatorcontrib>Chai, Menglong</creatorcontrib><creatorcontrib>Gao, Yan</creatorcontrib><creatorcontrib>Yuan, Bao</creatorcontrib><creatorcontrib>Chen, Chengzhen</creatorcontrib><creatorcontrib>Dai, Lisheng</creatorcontrib><creatorcontrib>Zhang, Jiabao</creatorcontrib><creatorcontrib>Ding, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell cycle (Georgetown, Tex.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yue, Yuan</au><au>Jiang, Hao</au><au>Yan, Shouqing</au><au>Fu, Yao</au><au>Liu, Chang</au><au>Sun, Xulei</au><au>Chai, Menglong</au><au>Gao, Yan</au><au>Yuan, Bao</au><au>Chen, Chengzhen</au><au>Dai, Lisheng</au><au>Zhang, Jiabao</au><au>Ding, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA-seq analysis provide new insights into mapk signaling of apolipoproteinciii-induced inflammation in porcine vascular endothelial cells</atitle><jtitle>Cell cycle (Georgetown, Tex.)</jtitle><addtitle>Cell Cycle</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>16</volume><issue>22</issue><spage>2230</spage><epage>2238</epage><pages>2230-2238</pages><issn>1538-4101</issn><eissn>1551-4005</eissn><abstract>Apolipoprotein CIII (ApoCIII) has been shown to be associated with the inflammatory response, but the mechanism of its inflammatory effects remains unclear. Because vascular endothelial cells (VECs) play a key role in the development of inflammation, the present study was performed to investigate inflammatory mechanisms induced by ApoCIII in VECs. In this study, we screened differentially expressed genes (DEGs) using RNA-sequencing. The results identified 390 up-regulated genes and 257 down-regulated genes. We performed GO functional classification and KEGG pathway analysis for DEGs. Analysis of sequencing data showed that 21 genes were related to the MAPK pathway. Finally, we investigated whether ApoCIII regulates the expression of pro-inflammatory cytokines via MAPK signaling pathway. The results showed that ApoCIII increased the expression levels of IL-6, TNF-α, VCAM-1 and ICAM-1 in VECs. ApoCIII activated the phosphorylation of ERK1/2 and p38 MAPK. An inhibitor of ERK1/2 and p38 MAPK decreased the protein levels of IL-6 and TNF-α. Our findings demonstrate that ApoCIII induces pro-inflammatory cytokine production in VECs via activation of ERK1/2 and p38 MAPK phosphorylation.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>28933987</pmid><doi>10.1080/15384101.2017.1373222</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3490-0755</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apolipoprotein C-III - pharmacology Endothelial Cells - metabolism Inflammation Interleukin-6 - metabolism Mitogen-Activated Protein Kinase 1 - genetics Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - genetics Mitogen-Activated Protein Kinase 3 - metabolism Phosphorylation Signal Transduction - physiology Swine Tumor Necrosis Factor-alpha - metabolism Vascular Cell Adhesion Molecule-1 - genetics Vascular Cell Adhesion Molecule-1 - metabolism |
| title | RNA-seq analysis provide new insights into mapk signaling of apolipoproteinciii-induced inflammation in porcine vascular endothelial cells |
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