Construction and expression of vectors encoding biologically active rodent gonadotropins
The gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are important hormones in vertebrate reproduction. The isolation of gonadotropins from the pituitary gland is sub-optimal, as the cross-contamination of one hormone with another is common and often results in the var...
Gespeichert in:
| Veröffentlicht in: | Journal of Reproduction and Development 2017, Vol.63(6), pp.605-609 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 609 |
|---|---|
| container_issue | 6 |
| container_start_page | 605 |
| container_title | Journal of Reproduction and Development |
| container_volume | 63 |
| creator | OHTA, Akihiko TSUNODA, Yuichiro TAMURA, Yoshihiko IINO, Kayoko NISHIMURA, Naoto NISHIHARA, Hiroto TAKANASHI, Haruka YOSHIDA, Saishu KATO, Takako KATO, Yukio |
| description | The gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are important hormones in vertebrate reproduction. The isolation of gonadotropins from the pituitary gland is sub-optimal, as the cross-contamination of one hormone with another is common and often results in the variation in the measured activity of LH and FSH. The production of recombinant hormones is, therefore, a viable approach to solve this problem. This study aimed to express recombinant rat, mouse, and mastomys FSH and LH in Chinese hamster ovary (CHO) cells. Their common α-subunits along with their hormone-specific β-subunits were encoded in a single mammalian expression vector. FSH from all three species was expressed, whereas expression was achieved only for the mouse LH. Immunohistochemistry for rat alpha subunit of glycoprotein hormone (αGSU) and LHβ and FSHβ subunits confirmed the production of the dimeric hormone in CHO cells. The recombinant rodent gonadotropins were confirmed to be biologically active; estradiol production was increased by recombinant FSH in granulosa cells, while recombinant LH increased testosterone production in Leydig cells. |
| doi_str_mv | 10.1262/jrd.2017-091 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5735272</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2014585598</sourcerecordid><originalsourceid>FETCH-LOGICAL-c602t-99c1ae2bb671d8f776d3d820700d0af2fdc03c2014a685012bc42ea6e813b1a03</originalsourceid><addsrcrecordid>eNpVkcFP7CAQxonR6D715tk08Wp1gJbSi8lz43uamHjRxBuhQCubCiuwG_3vpW9187xAmPnNNx8zCJ1guMCEkctF0BcEcFNCi3fQDNOKl1UFsItmOcJKzjE_QL9iXABQUrNqHx2QFiitoJ6h57l3MYWVSta7QjpdmPdlMDFOT98Xa6OSD7EwTnlt3VB01o9-sEqO40chc9naFMFr41IxeCe1T8EvrYtHaK-XYzTHX_chevpz8zi_Le8f_t7Nf9-XigFJZdsqLA3pOtZgzfumYZpqTqAB0CB70msFVOX_VZLxGjDpVEWMZIZj2mEJ9BBdbXSXq-7VaJWNBDmKZbCvMnwIL634mXH2RQx-LeqG1qQhWeDsSyD4t5WJSSz8KrjsWUxta17XLc_U-YZSwccYTL_tgEFMexB5D1NBI_LUM376v6st_D34DFxvgEVMcjBbQIZk1Wj-qTEq2HR8q26T6kUGYRz9BGg_ndc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2014585598</pqid></control><display><type>article</type><title>Construction and expression of vectors encoding biologically active rodent gonadotropins</title><source>J-STAGE Free</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>OHTA, Akihiko ; TSUNODA, Yuichiro ; TAMURA, Yoshihiko ; IINO, Kayoko ; NISHIMURA, Naoto ; NISHIHARA, Hiroto ; TAKANASHI, Haruka ; YOSHIDA, Saishu ; KATO, Takako ; KATO, Yukio</creator><creatorcontrib>OHTA, Akihiko ; TSUNODA, Yuichiro ; TAMURA, Yoshihiko ; IINO, Kayoko ; NISHIMURA, Naoto ; NISHIHARA, Hiroto ; TAKANASHI, Haruka ; YOSHIDA, Saishu ; KATO, Takako ; KATO, Yukio</creatorcontrib><description>The gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are important hormones in vertebrate reproduction. The isolation of gonadotropins from the pituitary gland is sub-optimal, as the cross-contamination of one hormone with another is common and often results in the variation in the measured activity of LH and FSH. The production of recombinant hormones is, therefore, a viable approach to solve this problem. This study aimed to express recombinant rat, mouse, and mastomys FSH and LH in Chinese hamster ovary (CHO) cells. Their common α-subunits along with their hormone-specific β-subunits were encoded in a single mammalian expression vector. FSH from all three species was expressed, whereas expression was achieved only for the mouse LH. Immunohistochemistry for rat alpha subunit of glycoprotein hormone (αGSU) and LHβ and FSHβ subunits confirmed the production of the dimeric hormone in CHO cells. The recombinant rodent gonadotropins were confirmed to be biologically active; estradiol production was increased by recombinant FSH in granulosa cells, while recombinant LH increased testosterone production in Leydig cells.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.2017-091</identifier><identifier>PMID: 29033405</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>17β-Estradiol ; Androgens ; Biological activity ; Contamination ; Expression vectors ; Follicle-stimulating hormone ; Gonadotropins ; Granulosa cells ; Hormones ; Immunohistochemistry ; Leydig cells ; Luteinizing hormone ; Mastomys ; Pituitary ; Pituitary (anterior) ; Recombinant ; Technology Report ; Testosterone</subject><ispartof>Journal of Reproduction and Development, 2017, Vol.63(6), pp.605-609</ispartof><rights>2017 Society for Reproduction and Development</rights><rights>Copyright Japan Science and Technology Agency 2017</rights><rights>2017 Society for Reproduction and Development 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c602t-99c1ae2bb671d8f776d3d820700d0af2fdc03c2014a685012bc42ea6e813b1a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735272/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735272/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29033405$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OHTA, Akihiko</creatorcontrib><creatorcontrib>TSUNODA, Yuichiro</creatorcontrib><creatorcontrib>TAMURA, Yoshihiko</creatorcontrib><creatorcontrib>IINO, Kayoko</creatorcontrib><creatorcontrib>NISHIMURA, Naoto</creatorcontrib><creatorcontrib>NISHIHARA, Hiroto</creatorcontrib><creatorcontrib>TAKANASHI, Haruka</creatorcontrib><creatorcontrib>YOSHIDA, Saishu</creatorcontrib><creatorcontrib>KATO, Takako</creatorcontrib><creatorcontrib>KATO, Yukio</creatorcontrib><title>Construction and expression of vectors encoding biologically active rodent gonadotropins</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>The gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are important hormones in vertebrate reproduction. The isolation of gonadotropins from the pituitary gland is sub-optimal, as the cross-contamination of one hormone with another is common and often results in the variation in the measured activity of LH and FSH. The production of recombinant hormones is, therefore, a viable approach to solve this problem. This study aimed to express recombinant rat, mouse, and mastomys FSH and LH in Chinese hamster ovary (CHO) cells. Their common α-subunits along with their hormone-specific β-subunits were encoded in a single mammalian expression vector. FSH from all three species was expressed, whereas expression was achieved only for the mouse LH. Immunohistochemistry for rat alpha subunit of glycoprotein hormone (αGSU) and LHβ and FSHβ subunits confirmed the production of the dimeric hormone in CHO cells. The recombinant rodent gonadotropins were confirmed to be biologically active; estradiol production was increased by recombinant FSH in granulosa cells, while recombinant LH increased testosterone production in Leydig cells.</description><subject>17β-Estradiol</subject><subject>Androgens</subject><subject>Biological activity</subject><subject>Contamination</subject><subject>Expression vectors</subject><subject>Follicle-stimulating hormone</subject><subject>Gonadotropins</subject><subject>Granulosa cells</subject><subject>Hormones</subject><subject>Immunohistochemistry</subject><subject>Leydig cells</subject><subject>Luteinizing hormone</subject><subject>Mastomys</subject><subject>Pituitary</subject><subject>Pituitary (anterior)</subject><subject>Recombinant</subject><subject>Technology Report</subject><subject>Testosterone</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpVkcFP7CAQxonR6D715tk08Wp1gJbSi8lz43uamHjRxBuhQCubCiuwG_3vpW9187xAmPnNNx8zCJ1guMCEkctF0BcEcFNCi3fQDNOKl1UFsItmOcJKzjE_QL9iXABQUrNqHx2QFiitoJ6h57l3MYWVSta7QjpdmPdlMDFOT98Xa6OSD7EwTnlt3VB01o9-sEqO40chc9naFMFr41IxeCe1T8EvrYtHaK-XYzTHX_chevpz8zi_Le8f_t7Nf9-XigFJZdsqLA3pOtZgzfumYZpqTqAB0CB70msFVOX_VZLxGjDpVEWMZIZj2mEJ9BBdbXSXq-7VaJWNBDmKZbCvMnwIL634mXH2RQx-LeqG1qQhWeDsSyD4t5WJSSz8KrjsWUxta17XLc_U-YZSwccYTL_tgEFMexB5D1NBI_LUM376v6st_D34DFxvgEVMcjBbQIZk1Wj-qTEq2HR8q26T6kUGYRz9BGg_ndc</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>OHTA, Akihiko</creator><creator>TSUNODA, Yuichiro</creator><creator>TAMURA, Yoshihiko</creator><creator>IINO, Kayoko</creator><creator>NISHIMURA, Naoto</creator><creator>NISHIHARA, Hiroto</creator><creator>TAKANASHI, Haruka</creator><creator>YOSHIDA, Saishu</creator><creator>KATO, Takako</creator><creator>KATO, Yukio</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><general>Japan Science and Technology Agency</general><general>The Society for Reproduction and Development</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>2017</creationdate><title>Construction and expression of vectors encoding biologically active rodent gonadotropins</title><author>OHTA, Akihiko ; TSUNODA, Yuichiro ; TAMURA, Yoshihiko ; IINO, Kayoko ; NISHIMURA, Naoto ; NISHIHARA, Hiroto ; TAKANASHI, Haruka ; YOSHIDA, Saishu ; KATO, Takako ; KATO, Yukio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c602t-99c1ae2bb671d8f776d3d820700d0af2fdc03c2014a685012bc42ea6e813b1a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>17β-Estradiol</topic><topic>Androgens</topic><topic>Biological activity</topic><topic>Contamination</topic><topic>Expression vectors</topic><topic>Follicle-stimulating hormone</topic><topic>Gonadotropins</topic><topic>Granulosa cells</topic><topic>Hormones</topic><topic>Immunohistochemistry</topic><topic>Leydig cells</topic><topic>Luteinizing hormone</topic><topic>Mastomys</topic><topic>Pituitary</topic><topic>Pituitary (anterior)</topic><topic>Recombinant</topic><topic>Technology Report</topic><topic>Testosterone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OHTA, Akihiko</creatorcontrib><creatorcontrib>TSUNODA, Yuichiro</creatorcontrib><creatorcontrib>TAMURA, Yoshihiko</creatorcontrib><creatorcontrib>IINO, Kayoko</creatorcontrib><creatorcontrib>NISHIMURA, Naoto</creatorcontrib><creatorcontrib>NISHIHARA, Hiroto</creatorcontrib><creatorcontrib>TAKANASHI, Haruka</creatorcontrib><creatorcontrib>YOSHIDA, Saishu</creatorcontrib><creatorcontrib>KATO, Takako</creatorcontrib><creatorcontrib>KATO, Yukio</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OHTA, Akihiko</au><au>TSUNODA, Yuichiro</au><au>TAMURA, Yoshihiko</au><au>IINO, Kayoko</au><au>NISHIMURA, Naoto</au><au>NISHIHARA, Hiroto</au><au>TAKANASHI, Haruka</au><au>YOSHIDA, Saishu</au><au>KATO, Takako</au><au>KATO, Yukio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction and expression of vectors encoding biologically active rodent gonadotropins</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2017</date><risdate>2017</risdate><volume>63</volume><issue>6</issue><spage>605</spage><epage>609</epage><pages>605-609</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>The gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), are important hormones in vertebrate reproduction. The isolation of gonadotropins from the pituitary gland is sub-optimal, as the cross-contamination of one hormone with another is common and often results in the variation in the measured activity of LH and FSH. The production of recombinant hormones is, therefore, a viable approach to solve this problem. This study aimed to express recombinant rat, mouse, and mastomys FSH and LH in Chinese hamster ovary (CHO) cells. Their common α-subunits along with their hormone-specific β-subunits were encoded in a single mammalian expression vector. FSH from all three species was expressed, whereas expression was achieved only for the mouse LH. Immunohistochemistry for rat alpha subunit of glycoprotein hormone (αGSU) and LHβ and FSHβ subunits confirmed the production of the dimeric hormone in CHO cells. The recombinant rodent gonadotropins were confirmed to be biologically active; estradiol production was increased by recombinant FSH in granulosa cells, while recombinant LH increased testosterone production in Leydig cells.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>29033405</pmid><doi>10.1262/jrd.2017-091</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8818 |
| ispartof | Journal of Reproduction and Development, 2017, Vol.63(6), pp.605-609 |
| issn | 0916-8818 1348-4400 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5735272 |
| source | J-STAGE Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | 17β-Estradiol Androgens Biological activity Contamination Expression vectors Follicle-stimulating hormone Gonadotropins Granulosa cells Hormones Immunohistochemistry Leydig cells Luteinizing hormone Mastomys Pituitary Pituitary (anterior) Recombinant Technology Report Testosterone |
| title | Construction and expression of vectors encoding biologically active rodent gonadotropins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T12%3A31%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Construction%20and%20expression%20of%20vectors%20encoding%20biologically%20active%20rodent%20gonadotropins&rft.jtitle=Journal%20of%20Reproduction%20and%20Development&rft.au=OHTA,%20Akihiko&rft.date=2017&rft.volume=63&rft.issue=6&rft.spage=605&rft.epage=609&rft.pages=605-609&rft.issn=0916-8818&rft.eissn=1348-4400&rft_id=info:doi/10.1262/jrd.2017-091&rft_dat=%3Cproquest_pubme%3E2014585598%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2014585598&rft_id=info:pmid/29033405&rfr_iscdi=true |