Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)

Transcriptomic profiling of ex vivo cultured human limbal epithelial stem cells (hLESCs) will foster better understanding of corneal physiology and novel treatment paradigms to limbal stem cell deficiency (LSCD). However, currently such profiling studies are hampered due to difficulties with produci...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biological procedures online 2017-12, Vol.19 (1), p.15-15, Article 15
Hauptverfasser:	Liu, Lei, Nielsen, Frederik Mølgaard, Riis, Simone Elkjær, Emmersen, Jeppe, Fink, Trine, Hjortdal, Jesper Østergaard, Bath, Chris, Zachar, Vladimir
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Fluorescence Genomics Messenger RNA Methodology RNA sequencing Stem cells
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!